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The map label for this gene is rnfF [H]

Identifier: 71905651

GI number: 71905651

Start: 12950

End: 13990

Strand: Reverse

Name: rnfF [H]

Synonym: Daro_0009

Alternate gene names: 71905651

Gene position: 13990-12950 (Counterclockwise)

Preceding gene: 71905652

Following gene: 71905650

Centisome position: 0.31

GC content: 60.33

Gene sequence:

>1041_bases
ATGCGGGCTGTCCTGAATCTTTTCCTGTCTGGCGTTTGCGTACTGCTGCTTGCCAGCTGTGGTCGGACGCAGCTGCAAGA
ACAGCAGGCCTACGTCTTTGGTACCCGTGTTGAGGTGCTGGTCGTCAGCGAGGATCCGGAACAGGGCCGCAAAGCGATTG
CCGCCGTCTTGCGCGAATTCGACCGCCTGCACCGCGCCTACCACGCTTGGCAGGACTCGGAACTGATGGCCTTGAACACG
GCATTTGCCCAGGGAAAAACCCGACAGGTCAGCCCTGAACTCGCCGCCTTCGTTCAGGAAGCACAGGCCCTTTCCCAACA
GGGCGACACCCTGTTCGATCCCGGCATTGGTCAGTTGATCAAACTGTGGGGCTTCCAGGCCGACGAATTCAAGGCAGAAC
TGCCTGCTGCAGCCGATATCAAGGCCTGGTTGGCCAGCAAGCCATCCATTGCCGACGTCGTGATCGACGGCACCAATATC
CGCAGCCGCAATCGTAACGTTGCCCTCGATTTCGGCGGCTACCTGAAGGGTGTCGCCCTTGATCGCGCCTCGGCCATCCT
CCACGCTCAAGGTATCCACAACGCCCTGATCAACATCGGCGGCAATGTCATGGCGCTGGGCAGCAAGGAAGGCAAGAAGT
GGCGCGTCGGCATCCAGCATCCACGTCAGCCGGGTCCGATGGCCACGGTCACGCTCGATGACGGCGAAGCGATCGGCACC
TCCGGCGACTATCAACGCTTCTTCGAGGTCGACGGACGACGTTACGCCCACCTGCTCGATCCTCGCACCGGCTACCCGGT
GGAACACACGCAGGCTGTCACGGTGCTCATCCCCAAGGGGCCAAAAGCAGGCACCTTGTCCGATGCGGCCTCCAAGCCGA
TTTTCATTGCAGGACCGGATGGCTGGCGCGATATGGCGCGAAAAATGGGAACCAGTCTCGTTTTGCGCGTCGACCATAGC
AATCAGATTTTCGTCACCGAGGCACTGCGCCAGCGTCTTGAATTCATCGGCGCCCCCCCGAAACTCAACGTTGTCCAATA
A

Upstream 100 bases:

>100_bases
TGACCGAAATCAACGTCACCAGTCCAACCTGCATGGTCGAAATCCGCCAGCAGTCGGGCTTTGATGCGGCCGGCGCCTTC
ATCACGGCCATCGAGCACGC

Downstream 100 bases:

>100_bases
CTCAGGTGAGTGTTTCCTTGACACTTGTATGCCGGAAACGTATATCTCGCCACATTGATGCCAGAATAAAAATCCCAGCG
ACAACAAGGGTTATCGTCGA

Product: ApbE-like lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRAVLNLFLSGVCVLLLASCGRTQLQEQQAYVFGTRVEVLVVSEDPEQGRKAIAAVLREFDRLHRAYHAWQDSELMALNT
AFAQGKTRQVSPELAAFVQEAQALSQQGDTLFDPGIGQLIKLWGFQADEFKAELPAAADIKAWLASKPSIADVVIDGTNI
RSRNRNVALDFGGYLKGVALDRASAILHAQGIHNALINIGGNVMALGSKEGKKWRVGIQHPRQPGPMATVTLDDGEAIGT
SGDYQRFFEVDGRRYAHLLDPRTGYPVEHTQAVTVLIPKGPKAGTLSDAASKPIFIAGPDGWRDMARKMGTSLVLRVDHS
NQIFVTEALRQRLEFIGAPPKLNVVQ

Sequences:

>Translated_346_residues
MRAVLNLFLSGVCVLLLASCGRTQLQEQQAYVFGTRVEVLVVSEDPEQGRKAIAAVLREFDRLHRAYHAWQDSELMALNT
AFAQGKTRQVSPELAAFVQEAQALSQQGDTLFDPGIGQLIKLWGFQADEFKAELPAAADIKAWLASKPSIADVVIDGTNI
RSRNRNVALDFGGYLKGVALDRASAILHAQGIHNALINIGGNVMALGSKEGKKWRVGIQHPRQPGPMATVTLDDGEAIGT
SGDYQRFFEVDGRRYAHLLDPRTGYPVEHTQAVTVLIPKGPKAGTLSDAASKPIFIAGPDGWRDMARKMGTSLVLRVDHS
NQIFVTEALRQRLEFIGAPPKLNVVQ
>Mature_346_residues
MRAVLNLFLSGVCVLLLASCGRTQLQEQQAYVFGTRVEVLVVSEDPEQGRKAIAAVLREFDRLHRAYHAWQDSELMALNT
AFAQGKTRQVSPELAAFVQEAQALSQQGDTLFDPGIGQLIKLWGFQADEFKAELPAAADIKAWLASKPSIADVVIDGTNI
RSRNRNVALDFGGYLKGVALDRASAILHAQGIHNALINIGGNVMALGSKEGKKWRVGIQHPRQPGPMATVTLDDGEAIGT
SGDYQRFFEVDGRRYAHLLDPRTGYPVEHTQAVTVLIPKGPKAGTLSDAASKPIFIAGPDGWRDMARKMGTSLVLRVDHS
NQIFVTEALRQRLEFIGAPPKLNVVQ

Specific function: Required for nitrogen fixation [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rseC family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=272, Percent_Identity=27.9411764705882, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007359
- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE; PF04246 RseC_MucC [H]

EC number: NA

Molecular weight: Translated: 37743; Mature: 37743

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVLNLFLSGVCVLLLASCGRTQLQEQQAYVFGTRVEVLVVSEDPEQGRKAIAAVLREF
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECEEEEEEEECCCHHHHHHHHHHHHHH
DRLHRAYHAWQDSELMALNTAFAQGKTRQVSPELAAFVQEAQALSQQGDTLFDPGIGQLI
HHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KLWGFQADEFKAELPAAADIKAWLASKPSIADVVIDGTNIRSRNRNVALDFGGYLKGVAL
HHHCCCCHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHH
DRASAILHAQGIHNALINIGGNVMALGSKEGKKWRVGIQHPRQPGPMATVTLDDGEAIGT
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCC
SGDYQRFFEVDGRRYAHLLDPRTGYPVEHTQAVTVLIPKGPKAGTLSDAASKPIFIAGPD
CCCHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCEEEECCC
GWRDMARKMGTSLVLRVDHSNQIFVTEALRQRLEFIGAPPKLNVVQ
CHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRAVLNLFLSGVCVLLLASCGRTQLQEQQAYVFGTRVEVLVVSEDPEQGRKAIAAVLREF
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECEEEEEEEECCCHHHHHHHHHHHHHH
DRLHRAYHAWQDSELMALNTAFAQGKTRQVSPELAAFVQEAQALSQQGDTLFDPGIGQLI
HHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KLWGFQADEFKAELPAAADIKAWLASKPSIADVVIDGTNIRSRNRNVALDFGGYLKGVAL
HHHCCCCHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHH
DRASAILHAQGIHNALINIGGNVMALGSKEGKKWRVGIQHPRQPGPMATVTLDDGEAIGT
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCC
SGDYQRFFEVDGRRYAHLLDPRTGYPVEHTQAVTVLIPKGPKAGTLSDAASKPIFIAGPD
CCCHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCEEEECCC
GWRDMARKMGTSLVLRVDHSNQIFVTEALRQRLEFIGAPPKLNVVQ
CHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 2071578 [H]