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The map label for this gene is gshB [H]

Identifier: 71905652

GI number: 71905652

Start: 13975

End: 14994

Strand: Reverse

Name: gshB [H]

Synonym: Daro_0010

Alternate gene names: 71905652

Gene position: 14994-13975 (Counterclockwise)

Preceding gene: 71905653

Following gene: 71905651

Centisome position: 0.33

GC content: 61.47

Gene sequence:

>1020_bases
ATGGCTCAGCAACTGCACTTTGAAAAACACCTGCTCGGCGGCAGCAGCCAGTCCCTGAAACTGGCTTTTGTGCTCGATCC
GCTGGACCATCTGAAGGCTTGGAAAGATTCTTCGGTGGCGATGATGCGGGCTGCCGAGAAGCATGGTCACGAAGTATTCG
CCATCGACGCCGCAACACTCGGCTGGCGCAAACCGGATGATGCCCACGCCGGTGGCATCTCCGGCGAGGCGCTGCACCTG
CATCTGCGCCCGGACGACCACGACTGGTATCGCGAAACCGGCCGCGAATGGATGCCATTGACGGCCTTCGACGCCGTGAT
CATGCGCAAGGATCCGCCCTTCGATTTCGAATACCTGACCGCCACCTGGCTGCTTGAACGAGCCGAGGCCGACGGCGTAC
GGGTTTTCAACCGGCCACGGGCCCTGCGCGATCATTCGGAAAAGCTGGCGATCATGGAGTTCGCCCACTTTGCGCCGGCG
ACTGCGATCAGTCGTGACATGGCGCAAATCCACCACTTCATCGACGAGCAGCGAGACGTTATCCTCAAGCCACTTGACGG
CATGGGTGGCAGCCAGATTTTCCGTGTGCATCGCAATGACCCGAACCGCAACGTGATCGTCGAAACACTGACCCACGAAG
GGGCCAGGACCATCATGGCGCAGCGCTACGTGCCGGAAATCAGCCAGGGCGACAAACGTATTCTGCTCATCGCCGGCAAG
CCAGTGCCGTGGTGCCTGGCGCGTATCCCCAAGGCCGGTGAGACGCGCGGCAACCTGGCCGTCGGTGGCACCGGCGTCGC
TCAGGAACTGTCGGCCCGTGATCTGGAAATTGCCGAAACGCTGGGCCCCATTCTGTTCAAGCGCGGGCTGATGCTGGTCG
GCATCGACGTCATTGGCAACTTCCTGACCGAAATCAACGTCACCAGTCCAACCTGCATGGTCGAAATCCGCCAGCAGTCG
GGCTTTGATGCGGCCGGCGCCTTCATCACGGCCATCGAGCACGCATGCGGGCTGTCCTGA

Upstream 100 bases:

>100_bases
ACCCAACCGCTTCTACGCGTATGGCGTCGTCGGCCGCCTGGCCTGCCTGGCCGCTGCCATCGAGCTGGAACGCACTGCGC
CGGAAGACGTCTGAGTCGGC

Downstream 100 bases:

>100_bases
ATCTTTTCCTGTCTGGCGTTTGCGTACTGCTGCTTGCCAGCTGTGGTCGGACGCAGCTGCAAGAACAGCAGGCCTACGTC
TTTGGTACCCGTGTTGAGGT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAQQLHFEKHLLGGSSQSLKLAFVLDPLDHLKAWKDSSVAMMRAAEKHGHEVFAIDAATLGWRKPDDAHAGGISGEALHL
HLRPDDHDWYRETGREWMPLTAFDAVIMRKDPPFDFEYLTATWLLERAEADGVRVFNRPRALRDHSEKLAIMEFAHFAPA
TAISRDMAQIHHFIDEQRDVILKPLDGMGGSQIFRVHRNDPNRNVIVETLTHEGARTIMAQRYVPEISQGDKRILLIAGK
PVPWCLARIPKAGETRGNLAVGGTGVAQELSARDLEIAETLGPILFKRGLMLVGIDVIGNFLTEINVTSPTCMVEIRQQS
GFDAAGAFITAIEHACGLS

Sequences:

>Translated_339_residues
MAQQLHFEKHLLGGSSQSLKLAFVLDPLDHLKAWKDSSVAMMRAAEKHGHEVFAIDAATLGWRKPDDAHAGGISGEALHL
HLRPDDHDWYRETGREWMPLTAFDAVIMRKDPPFDFEYLTATWLLERAEADGVRVFNRPRALRDHSEKLAIMEFAHFAPA
TAISRDMAQIHHFIDEQRDVILKPLDGMGGSQIFRVHRNDPNRNVIVETLTHEGARTIMAQRYVPEISQGDKRILLIAGK
PVPWCLARIPKAGETRGNLAVGGTGVAQELSARDLEIAETLGPILFKRGLMLVGIDVIGNFLTEINVTSPTCMVEIRQQS
GFDAAGAFITAIEHACGLS
>Mature_338_residues
AQQLHFEKHLLGGSSQSLKLAFVLDPLDHLKAWKDSSVAMMRAAEKHGHEVFAIDAATLGWRKPDDAHAGGISGEALHLH
LRPDDHDWYRETGREWMPLTAFDAVIMRKDPPFDFEYLTATWLLERAEADGVRVFNRPRALRDHSEKLAIMEFAHFAPAT
AISRDMAQIHHFIDEQRDVILKPLDGMGGSQIFRVHRNDPNRNVIVETLTHEGARTIMAQRYVPEISQGDKRILLIAGKP
VPWCLARIPKAGETRGNLAVGGTGVAQELSARDLEIAETLGPILFKRGLMLVGIDVIGNFLTEINVTSPTCMVEIRQQSG
FDAAGAFITAIEHACGLS

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=319, Percent_Identity=45.7680250783699, Blast_Score=295, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 37644; Mature: 37512

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQLHFEKHLLGGSSQSLKLAFVLDPLDHLKAWKDSSVAMMRAAEKHGHEVFAIDAATL
CCCCHHHHHHHCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEHHC
GWRKPDDAHAGGISGEALHLHLRPDDHDWYRETGREWMPLTAFDAVIMRKDPPFDFEYLT
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHHEEECCCCCCCHHHHH
ATWLLERAEADGVRVFNRPRALRDHSEKLAIMEFAHFAPATAISRDMAQIHHFIDEQRDV
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCE
ILKPLDGMGGSQIFRVHRNDPNRNVIVETLTHEGARTIMAQRYVPEISQGDKRILLIAGK
EEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECC
PVPWCLARIPKAGETRGNLAVGGTGVAQELSARDLEIAETLGPILFKRGLMLVGIDVIGN
CCHHHHHHCCCCCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHHHCCHHEEEHHHHHH
FLTEINVTSPTCMVEIRQQSGFDAAGAFITAIEHACGLS
HHHHCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQQLHFEKHLLGGSSQSLKLAFVLDPLDHLKAWKDSSVAMMRAAEKHGHEVFAIDAATL
CCCHHHHHHHCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEHHC
GWRKPDDAHAGGISGEALHLHLRPDDHDWYRETGREWMPLTAFDAVIMRKDPPFDFEYLT
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHHEEECCCCCCCHHHHH
ATWLLERAEADGVRVFNRPRALRDHSEKLAIMEFAHFAPATAISRDMAQIHHFIDEQRDV
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCE
ILKPLDGMGGSQIFRVHRNDPNRNVIVETLTHEGARTIMAQRYVPEISQGDKRILLIAGK
EEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECC
PVPWCLARIPKAGETRGNLAVGGTGVAQELSARDLEIAETLGPILFKRGLMLVGIDVIGN
CCHHHHHHCCCCCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHHHCCHHEEEHHHHHH
FLTEINVTSPTCMVEIRQQSGFDAAGAFITAIEHACGLS
HHHHCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]