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The map label for this gene is metB [H]

Identifier: 71909960

GI number: 71909960

Start: 156335

End: 157525

Strand: Direct

Name: metB [H]

Synonym: M5005_Spy_0146

Alternate gene names: 71909960

Gene position: 156335-157525 (Clockwise)

Preceding gene: 71909956

Following gene: 161936127

Centisome position: 8.5

GC content: 42.23

Gene sequence:

>1191_bases
TTGTCTAAAAGAGAGTTCATTGGAGTTAACGCTATTGCGGAGGTGCACGAAATGAGAGAAAATACGACCTTATTACATGG
CTATACGGTTATTGACGAGTTTACAGGAGCAGCTTCAGTTCCCATCTATCAGACTTCGACGTTCCACAATTCAGAACTGT
ATTGTCCTAGTCAAAAACATTTGTACACACGCTTTTCAAACCCAACGACTGAAGCTTTAGAAGACGGGTTAGCCTGTTTA
GAAAAAGCGACTTATGCGGTGGCTTATGCATCTGGTATGGCTGCTATTTCAACTGTTTTGATGCTCTTAAAAGCTGGAGA
TCATGTGATTTTCCCATTGGAGGTCTATGGAGGGACTTGTCAATTTGCGACAGCTATTTTACCCAATTATCAGATTGAAA
CAAGCTTTGTAGATATGGCTGATTTAGCAACAGTAAAAGCAAGTATCAGGCCTAATACGCGTATGATTTATCTAGAGACA
CCGTCAAATCCGCTCTTGAAAATTTGTGATATTTCAGAGCTTGCGCAACTCGCCAAAGCTTATGGTGTGCTAACTGTGGC
TGATAATACCTTTATGACCAGTTTGTATCAAGAACCCTTAGCTATGGGTGTGGATATTGTAGTAGAAAGTGTGACCAAAT
TTATCAATGGTCATAGTGATGTGGTAGCTGGTTTAGCTGCTACCAATAATGAGGCTATCTACAACCAGCTCAAGCTTTTT
CAAAAAAACTTTGGAGCAATTGTTGGGGTCGAAGACGCTTGGCTGATTCTTCGTGGCATGAAAACTATGGGGATCCGTAT
GGAACAAGCGGTAAAAAATGCTCAGCAGCTCGCTAACTATTTAGCGAAGCACCCCAAAGTTCTTAAGGTACATTACCCTG
GCTTAGACAGTCATCCTAATCACGATACCCACCTGCAACAAGCTAAGAATGGTGGTGCTGTTTTATCCTTTGAGTTGGCT
TCTAAGGAGGAGCTGATGTCCTTTACCCATCGTATTCAGTTGCCGATTTTAGCCGTTAGTCTAGGTGGAGTAGAGTCCAT
TTTGTCACATCCAGCAACGATGTCTCATGCTTGTTTATCGTCACAAGCGCGGTTGGAACAAGGGGTTGTTGATGGTTTGC
TGCGCTTGTCTTGTGGTGTTGAAAATATCGAAGACTTATTGGCCGATTTTGAGCAAGCACTTGCTTTTTAA

Upstream 100 bases:

>100_bases
TTAAAAGGTGCCCACTACTTTTCTTTATTTTTCTTATAGGTTAGCAGATCACGCTTTTTTGAAGAGCTTTTTGGTTTGTG
CAACCATTGGGGTGGGACCA

Downstream 100 bases:

>100_bases
GTCTATTTGTCCAGATTGGCTCTGTTTACAGAGCAAGTCTGGCTTTTTTATTAGCTTTTTGTGAGGATGGTTGATGCTAA
AGCAGGGTTTAAAAATAGCT

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSKREFIGVNAIAEVHEMRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGLACL
EKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLET
PSNPLLKICDISELAQLAKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLF
QKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSFELA
SKEELMSFTHRIQLPILAVSLGGVESILSHPATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF

Sequences:

>Translated_396_residues
MSKREFIGVNAIAEVHEMRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGLACL
EKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLET
PSNPLLKICDISELAQLAKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLF
QKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSFELA
SKEELMSFTHRIQLPILAVSLGGVESILSHPATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF
>Mature_395_residues
SKREFIGVNAIAEVHEMRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGLACLE
KATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETP
SNPLLKICDISELAQLAKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLFQ
KNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSFELAS
KEELMSFTHRIQLPILAVSLGGVESILSHPATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF

Specific function: Catalyzes the transformation of cystathionine into homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=368, Percent_Identity=40.2173913043478, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI299473759, Length=368, Percent_Identity=35.3260869565217, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI25453487, Length=367, Percent_Identity=35.6948228882834, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1790375, Length=365, Percent_Identity=39.1780821917808, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1789383, Length=359, Percent_Identity=26.7409470752089, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17538055, Length=358, Percent_Identity=40.2234636871508, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17560056, Length=360, Percent_Identity=40.8333333333333, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71980708, Length=365, Percent_Identity=37.8082191780822, Blast_Score=247, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=369, Percent_Identity=42.0054200542005, Blast_Score=287, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6321254, Length=387, Percent_Identity=33.5917312661499, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=318, Percent_Identity=31.4465408805031, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=414, Percent_Identity=28.0193236714976, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=28.4615384615385, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322590, Length=255, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=268, Percent_Identity=27.6119402985075, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323557, Length=358, Percent_Identity=24.5810055865922, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=38.8888888888889, Blast_Score=277, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 43245; Mature: 43114

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKREFIGVNAIAEVHEMRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKH
CCCCCEECHHHHHHHHHHHHCCEEEEEEEHHHHCCCCCCCCEEEECCCCCCEEECCCHHH
LYTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGTC
HEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCH
QFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELAQLAKA
HHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHHHHHHH
YGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLF
HCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
QKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPN
HHHCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
HDTHLQQAKNGGAVLSFELASKEELMSFTHRIQLPILAVSLGGVESILSHPATMSHACLS
CCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHHHHHH
SQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKREFIGVNAIAEVHEMRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKH
CCCCEECHHHHHHHHHHHHCCEEEEEEEHHHHCCCCCCCCEEEECCCCCCEEECCCHHH
LYTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGTC
HEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCH
QFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELAQLAKA
HHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHHHHHHH
YGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLF
HCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
QKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPN
HHHCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
HDTHLQQAKNGGAVLSFELASKEELMSFTHRIQLPILAVSLGGVESILSHPATMSHACLS
CCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHHHHHH
SQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]