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The map label for this gene is leuS [H]

Identifier: 161936127

GI number: 161936127

Start: 157736

End: 160237

Strand: Direct

Name: leuS [H]

Synonym: M5005_Spy_0147

Alternate gene names: 161936127

Gene position: 157736-160237 (Clockwise)

Preceding gene: 71909960

Following gene: 71909962

Centisome position: 8.58

GC content: 46.56

Gene sequence:

>2502_bases
ATGACATTTTACGATCACACCGCTATTGAACCCAAATGGCAAGCCTTTTGGGCAGATAATCACACCTTTAAAACAGGAAC
AGACGCATCAAAACCTAAGTTTTATGCCCTAGATATGTTCCCATATCCATCAGGAGCTGGTCTCCACGTTGGTCACCCAG
AAGGCTATACAGCAACTGATATTCTTAGCCGTTTCAAACGTGCCCAAGGTCACAATGTCCTCCACCCAATGGGTTGGGAT
GCCTTTGGACTTCCTGCTGAGCAGTACGCTATGGATACTGGTAATGATCCAGCCGAGTTTACAGCTGAAAACATCGCTAA
CTTCAAACGCCAGATTAATGCTCTTGGCTTCTCATACGACTGGGACCGTGAAGTTAACACAACTGACCCTAACTACTACA
AGTGGACGCAGTGGATCTTTACCAAGCTTTATGAAAAAGGTCTAGCCTACGAAGCTGAAGTTCCCGTTAACTGGGTTGAA
GAATTGGGAACAGCTATCGCCAACGAAGAGGTTCTTCCAGATGGGACATCAGAACGTGGCGGCTATCCAGTTGTCAGAAA
ACCCATGCGCCAATGGATGCTCAAAATCACTGCCTATGCAGAGCGCTTGCTAGAAGACTTGGAAGAAGTTGACTGGCCAG
AATCAATCAAGGACATGCAACGCAACTGGATTGGTAAATCAACTGGCGCTAATGTAACCTTCAAGGTTAAGGATACAGAT
AAGGACTTCACTGTCTTTACAACACGTCCTGACACGCTTTTTGGTGCAACTTACGCCGTTCTTGCTCCTGAGCATGCCTT
GGTTGATGCTATTACGACATCTGACCAGGCAGAAGCCGTGGCTGACTACAAACGTCAAGCAAGTCTCAAATCAGACCTTG
CTCGTACAGACCTTGCCAAAGAAAAAACTGGTGTCTGGACGGGTAGCTATGCGATTAACCCGGTCAACGGCAAAGAAATG
CCAGTTTGGATTGCGGACTATGTCCTTGCTAGCTACGGTACTGGTGCTATCATGGCTGTTCCAGCCCACGATGAACGTGA
CTGGGAATTCGCAAAACAATTTAACCTAGACATCATCCCTGTTCTTGAAGGTGGTAATGTTGAAGAGGCTGCCTTTACAG
AAGACGGTCTTCACATCAACTCCGACTTCCTAGATGGTCTTGACAAAGCGTCAGCTATTGCCAAAATGGTCGAATGGCTT
GAAGCAGAAGGCGTCGGTAATGAAAATGTCACTTACCGTCTCCGTGACTGGCTCTTTAGCCGTCAACGTTACTGGGGTGA
GCCAATCCCAATCATCCACTGGGAAGATGGGACATCAACAGCGGTTCCTGAGAGTGAATTGCCATTGGTTCTTCCAGTAA
CTAAGGATATTCGTCCTTCAGGTACTGGTGAGAGCCCGCTTGCTAACGTGACAGACTGGTTGGAAGTTACCCGTGAAGAT
GGTGTTAAAGGTCGTCGTGAGACTAACACCATGCCACAATGGGCAGGTTCAAGCTGGTATTATCTTCGTTACATCGATCC
ACACAACACCGAAAAACTGGCTGATGAGGAACTCCTCAAACAATGGTTGCCAGTTGATATCTATGTCGGTGGTGCAGAGC
ATGCCGTGCTTCACCTTCTCTACGCTCGCTTCTGGCACAAGGTTCTTTACGATCTCGGTGTTGTACCTACCAAAGAGCCA
TTCCAAAAACTCTTTAACCAAGGGATGATTTTGGGGACAAGCTACCGCGATAGCCGTGGAGCGCTTGTAGCGACTGACAA
GGTTGAAAAACGTGATGGCAGCTTCTTCCATGTGGAAACTGGGGAAGAGCTTGAACAAGCACCAGCTAAGATGTCTAAAT
CACTTAAGAACGTCGTCAACCCAGATGATGTTGTTGAGCAGTACGGTGCTGACACCCTTCGTGTTTACGAGATGTTCATG
GGACCACTTGACGCTTCCATCGCTTGGTCTGAAGAAGGTCTTGAAGGCTCACGTAAGTTCTTGGACCGTGTTTACCGTTT
GATCACGACTAAAGAGATCACTGAGGAAAATAGCGGAGCCCTTGACAAGGTTTACAACGAAACCGTTAAGGCCGTTACTG
AGCAAGTTGACCAAATGAAATTCAACACAGCTATCGCTCAACTCATGGTATTTGTCAATGCAGCCAACAAGGAAGACAAA
CTCTTTAGCGACTATGCCAAAGGCTTTGTTCAATTGATTGCACCATTTGCACCGCACTTAGGTGAGGAACTCTGGCAAGC
CTTGACAGCCTCAGGTGAGTCAATCTCTTACGTGCCATGGCCAAGTTATGATGAAAGCAAATTGGTTGAAAACGATGTGG
AAATCGTTGTCCAAATCAAAGGTAAGGTCAAAGCTAAACTTGTAGTTGCTAAAGACCTTTCACGCGAAGAATTACAAGAA
GTTGCCCTTGCTAACGAAAAAGTCCAAGCCGAAATCGCAGGCAAAGACATCATCAAAGTCATCGCAGTACCAAACAAACT
CGTTAATATTGTTATCAAATAG

Upstream 100 bases:

>100_bases
AGTCTCAAAAGCCAAGGTGCTAGTCATGTCTTGACCATTCCTAAAAGCTTCATCTCATGCTATAATAAGAAGACTAGATT
TTTTACATGGGAGATTTCAC

Downstream 100 bases:

>100_bases
TTTGAGGTGACACAGCCCTTCAGTGGTTATTTGCGTCACGTCACAGTTAATGCCAAAGAGCTGATCTATTCAAAAAGCAA
CTCATCTAGAAAAACCACAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQAEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEM
PVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASAIAKMVEWL
EAEGVGNENVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEP
FQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREELQE
VALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK

Sequences:

>Translated_833_residues
MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQAEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEM
PVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASAIAKMVEWL
EAEGVGNENVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEP
FQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREELQE
VALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK
>Mature_832_residues
TFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDA
FGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEE
LGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTDK
DFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQAEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEMP
VWIADYVLASYGTGAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASAIAKMVEWLE
AEGVGNENVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTREDG
VKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPF
QKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMG
PLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKL
FSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREELQEV
ALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=858, Percent_Identity=34.3822843822844, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI94721239, Length=183, Percent_Identity=28.4153005464481, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI94721241, Length=183, Percent_Identity=28.4153005464481, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786861, Length=866, Percent_Identity=42.378752886836, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=387, Percent_Identity=25.8397932816537, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71997510, Length=775, Percent_Identity=29.5483870967742, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71997517, Length=775, Percent_Identity=29.5483870967742, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=29.0322580645161, Blast_Score=154, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323414, Length=852, Percent_Identity=35.3286384976526, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321531, Length=434, Percent_Identity=25.1152073732719, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319395, Length=180, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21355409, Length=757, Percent_Identity=34.4782034346103, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17864482, Length=456, Percent_Identity=23.0263157894737, Blast_Score=101, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653289, Length=456, Percent_Identity=23.0263157894737, Blast_Score=101, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281366294, Length=388, Percent_Identity=25.2577319587629, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 93865; Mature: 93734

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCCCCEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQAEAVADYKRQASLKSDLARTDLAK
CCEEEEECCCCCHHCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGSYAINPVNGKEMPVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFNLDIIP
HHCCEEECCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCEEEE
VLEGGNVEEAAFTEDGLHINSDFLDGLDKASAIAKMVEWLEAEGVGNENVTYRLRDWLFS
EECCCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
YARFWHKVLYDLGVVPTKEPFQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCEEEEEEEC
GKVKAKLVVAKDLSREELQEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK
CCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHCCCHHEEEEECCCHHEEEECC
>Mature Secondary Structure 
TFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCCCEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQAEAVADYKRQASLKSDLARTDLAK
CCEEEEECCCCCHHCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGSYAINPVNGKEMPVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFNLDIIP
HHCCEEECCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCEEEE
VLEGGNVEEAAFTEDGLHINSDFLDGLDKASAIAKMVEWLEAEGVGNENVTYRLRDWLFS
EECCCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
YARFWHKVLYDLGVVPTKEPFQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCEEEEEEEC
GKVKAKLVVAKDLSREELQEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK
CCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHCCCHHEEEEECCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA