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The map label for this gene is purD [H]

Identifier: 71909843

GI number: 71909843

Start: 46998

End: 48353

Strand: Direct

Name: purD [H]

Synonym: M5005_Spy_0029

Alternate gene names: 71909843

Gene position: 46998-48353 (Clockwise)

Preceding gene: 71909841

Following gene: 71909844

Centisome position: 2.56

GC content: 47.05

Gene sequence:

>1356_bases
ATGTTTCAATTATTGATAACTAATACATTATGTAAAAAAGCGAACGTTTATGATATAATATCTAATTATAATACGAAAGA
GGTGGATGTTTTGAAATTACTTGTTGTTGGTTCAGGTGGTCGTGAACATGCGATTGCTAAGAAGTTGTTAGCGTCTAAGG
GTGTGGATCAGGTTTTTGTGGCACCTGGTAATGATGGTATGACCTTGGATGGTCTGGACTTGGTGAATATAGTAGTTTCC
GAACATTCCAGGTTGATTGCCTTTGCTAAGGAGAATGAGATTTCTTGGGCCTTTATTGGTCCTGATGATGCGCTAGCAGC
TGGTATCGTTGATGATTTCAACAGTGCTGGACTCAGAGCTTTTGGTCCAACAAAGGCAGCAGCGGAGCTAGAGTGGTCAA
AAGACTTTGCCAAGGAAATCATGGTCAAATACAATGTCCCAACAGCAGCCTATGGCACATTTTCAGACTTTGAAAAAGCC
AAAGCCTACATCGAAGAGCAGGGCGCACCTATCGTGGTCAAGGCTGATGGCTTGGCGCTAGGTAAGGGTGTGGTCGTGGC
TGAAACCGTTGAGCAGGCGGTAGAGGCGGCGCAAGAGATGCTTCTGGACAATAAGTTTGGCGATTCAGGAGCGCGTGTGG
TTATCGAGGAATTCTTGGATGGCGAGGAGTTTTCCCTCTTTGCCTTTGCTAATGGCGATAAGTTCTACATCATGCCGACA
GCTCAGGATCACAAGCGTGCCTTTGACGGAGACAAGGGGCCAAACACTGGTGGTATGGGTGCCTATGCGCCAGTTCCTCA
TCTACCTCAGAGCGTGGTAGACACAGCGGTTGAGATGATCGTTAGGCCTGTCCTTGAGGGCATGGTTGCCGAAGGGCGTC
CTTATCTAGGTGTCCTCTATGTGGGGCTTATCCTGACAGCTGATGGACCTAAGGTTATCGAGTTCAACTCACGTTTCGGT
GACCCTGAAACTCAGATTATCCTCCCTCGCCTGACTTCTGATTTCGCTCAGAATATTGACGATATCATGATGGGCATTGA
GCCTTACATCACTTGGCAGAAGGACGGTGTGACTTTGGGTGTTGTCGTTGCCTCAGAAGGCTATCCATTCGATTATGAGA
AAGGTGTGCCACTGCCTGAAAAGACCGACGGCGATATCATCACCTACTACGCAGGAGTTAAGTTTTCGGAAAATAGCGAG
CTACTTCTCTCAAACGGAGGACGTGTCTATATGCTCGTCACCACAGAAGACAGCGTCAAAGCAGGGCAGGACAAAATCTA
TACCCAACTGGCCCAACAAGACACAACAGGTCTCTTCTACCGAAATGATATCGGAAGCAAAGCTATAAGAGAATAA

Upstream 100 bases:

>100_bases
TATTTTGAAAATTGGCTTTACTTGATATTAAATAACTGTTTGATTGGCTAATAGAGTGTTCCTTATTTCAAGAATTGTGG
TCTACTATCACATTTTTACA

Downstream 100 bases:

>100_bases
GACTAACCATTCAAATATTAGCGTCAGCAATCAGCGACCGAAGGTCGCTCCAAAAAGAAAGAAGAAAGGAACTGAACACG
GGACTAATTTCCCAGGAAAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MFQLLITNTLCKKANVYDIISNYNTKEVDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVS
EHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKA
KAYIEEQGAPIVVKADGLALGKGVVVAETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPT
AQDHKRAFDGDKGPNTGGMGAYAPVPHLPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFG
DPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSE
LLLSNGGRVYMLVTTEDSVKAGQDKIYTQLAQQDTTGLFYRNDIGSKAIRE

Sequences:

>Translated_451_residues
MFQLLITNTLCKKANVYDIISNYNTKEVDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVS
EHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKA
KAYIEEQGAPIVVKADGLALGKGVVVAETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPT
AQDHKRAFDGDKGPNTGGMGAYAPVPHLPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFG
DPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSE
LLLSNGGRVYMLVTTEDSVKAGQDKIYTQLAQQDTTGLFYRNDIGSKAIRE
>Mature_451_residues
MFQLLITNTLCKKANVYDIISNYNTKEVDVLKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDLVNIVVS
EHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKA
KAYIEEQGAPIVVKADGLALGKGVVVAETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPT
AQDHKRAFDGDKGPNTGGMGAYAPVPHLPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILTADGPKVIEFNSRFG
DPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSE
LLLSNGGRVYMLVTTEDSVKAGQDKIYTQLAQQDTTGLFYRNDIGSKAIRE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=43.0588235294118, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=43.0588235294118, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=43.0588235294118, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=43.0588235294118, Blast_Score=349, Evalue=3e-96,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=45.4332552693208, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=37.4117647058824, Blast_Score=244, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=38.9261744966443, Blast_Score=302, Evalue=6e-83,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=42.5925925925926, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=42.5925925925926, Blast_Score=304, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 48937; Mature: 48937

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLLITNTLCKKANVYDIISNYNTKEVDVLKLLVVGSGGREHAIAKKLLASKGVDQVFV
CEEEEEEHHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEE
APGNDGMTLDGLDLVNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRA
ECCCCCCEECCHHHHHHHHHCCCEEEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCEE
FGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLAL
CCCCCCHHCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEE
GKGVVVAETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPT
CCCEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCEEEEECC
AQDHKRAFDGDKGPNTGGMGAYAPVPHLPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLY
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEE
VGLILTADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLG
EEEEEECCCCEEEEECCCCCCCCCEEEEHHHHHHHHHCHHHHHHCCCCEEEEECCCEEEE
VVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSELLLSNGGRVYMLVTTEDSVK
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEECEEECCCCCEEEECCCEEEEEEECCCCCC
AGQDKIYTQLAQQDTTGLFYRNDIGSKAIRE
CCHHHHHHHHHHCCCCCEEEECCCCCHHHCC
>Mature Secondary Structure
MFQLLITNTLCKKANVYDIISNYNTKEVDVLKLLVVGSGGREHAIAKKLLASKGVDQVFV
CEEEEEEHHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEE
APGNDGMTLDGLDLVNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRA
ECCCCCCEECCHHHHHHHHHCCCEEEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCEE
FGPTKAAAELEWSKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLAL
CCCCCCHHCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEE
GKGVVVAETVEQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPT
CCCEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCEEEEECC
AQDHKRAFDGDKGPNTGGMGAYAPVPHLPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLY
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEE
VGLILTADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLG
EEEEEECCCCEEEEECCCCCCCCCEEEEHHHHHHHHHCHHHHHHCCCCEEEEECCCEEEE
VVVASEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSELLLSNGGRVYMLVTTEDSVK
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEECEEECCCCCEEEECCCEEEEEEECCCCCC
AGQDKIYTQLAQQDTTGLFYRNDIGSKAIRE
CCHHHHHHHHHHCCCCCEEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]