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The map label for this gene is aceF [H]

Identifier: 71891940

GI number: 71891940

Start: 195617

End: 196888

Strand: Reverse

Name: aceF [H]

Synonym: BPEN_157

Alternate gene names: 71891940

Gene position: 196888-195617 (Counterclockwise)

Preceding gene: 71891941

Following gene: 71891939

Centisome position: 24.87

GC content: 32.0

Gene sequence:

>1272_bases
ATGACAATTGAAATTAATATACCAAATATTGGAGAGGATGAATTAGAAGTTACAGAAATAATGGTAAAAATAGGAGATAA
TATCAATGCTAATCAGCCACTTATTATAATTGAAGGTGATAAATCATCTATGGAAATACCATCTTCCTGTTCTGGTATTG
TTACTAAAATTTATGTCCATGTTGGAGATAAAGTACATACAGGATCTTTGATTTTACTACTTGATGTGCGAAATCATACT
AATGCTTTTACTATTAACGATAAAAAAAATGTTGTTCCTTCTCCTTATATTGTAACAAACAATGATACAAGAAAAGGAGC
GATATGTAACGATATACACCATGATGCTGCGATACATGCTACACCGTTAGTACGTCATATGGCTCGTACATTTGGAATAG
ATTTGTCAAAAATAAAAGGTAGTGGCCGTAAGGGACGTATTTTAAAAGAAGATATTCAAAATTATATAAAAAATATCTCA
ATGTATTATACAAACTGTATGTCATCCATGCAATCTGATCAATTACTACCCATATTATCTTGGCCAAAGATAGACTTCAG
TAAATTTGGCGATATTACAACCGTAATGCTAAGCAAAATACAAAAAACTTCTGGTGCAAATTTACAAAGAAATTGGATTA
TGCTGCCGCATGTAACGCAATTTGATGAAGCTGATATTACCGATTTAGAAAGTTTCAGAAAACAACAAAACATTGACATT
GAAAAGAAAAAAATAAATTGCAAAATTACACTTTTAGTTTTTGTTATGAAAGCAGTTGCAAAAGCATTAGAAGAGTTGCC
ACGATTCAATAGTTCTTTATCTCAAGACGGCGAAACACTAATTTTAAAAAAATATATTAATATTGGCATAGCAGTGGATA
CTCCTAAAGGTTTATTAGTGCCTGTTCTCCATGATGTAAACAGGAAAGGTATTATTTTATTATCACAAGAGTTAGAAGAG
CTTTCAAAGAAAGCTCGTACTGGGAATCAATTGACTCCTGCTAATATGCAAGGAGGAAGTTTTACTATATCCAATTTGGG
GGGTATAGGAGGTACAGCTTTTACTCCAATTGTTAATGTTCCAGAAGTAGCTATTTTAGGCATTTCTAAATCTTTTATAA
AACCAGTGTGGACCGGAAAAAAATTTACTCCGCGTTTAATGCTACCTTTGTCATTATCATATGATCATCGTGTTATTGAC
GGAGCTGATGGTGCTCGATTTATGACGCTCATTAATAAAATAATTGCTGACACAAGACTATTATCTATGTAA

Upstream 100 bases:

>100_bases
AAAAAGGCCATATTCACGCTGATGTTGTTCTAAACGCTATCAAAATATTTGATATTGACCCTGAAAAAATTAATCCACGC
CTAATATAAGAGGTAATACG

Downstream 100 bases:

>100_bases
ATTGCTAATCATATAAAAATAGATTTAAATATTTCACTTTTTTAAAAATTGTCTTTCCGTCCTTTATGGTTAAAGATAAT
CTCGCTTAAATTAAGTTATG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTIEINIPNIGEDELEVTEIMVKIGDNINANQPLIIIEGDKSSMEIPSSCSGIVTKIYVHVGDKVHTGSLILLLDVRNHT
NAFTINDKKNVVPSPYIVTNNDTRKGAICNDIHHDAAIHATPLVRHMARTFGIDLSKIKGSGRKGRILKEDIQNYIKNIS
MYYTNCMSSMQSDQLLPILSWPKIDFSKFGDITTVMLSKIQKTSGANLQRNWIMLPHVTQFDEADITDLESFRKQQNIDI
EKKKINCKITLLVFVMKAVAKALEELPRFNSSLSQDGETLILKKYINIGIAVDTPKGLLVPVLHDVNRKGIILLSQELEE
LSKKARTGNQLTPANMQGGSFTISNLGGIGGTAFTPIVNVPEVAILGISKSFIKPVWTGKKFTPRLMLPLSLSYDHRVID
GADGARFMTLINKIIADTRLLSM

Sequences:

>Translated_423_residues
MTIEINIPNIGEDELEVTEIMVKIGDNINANQPLIIIEGDKSSMEIPSSCSGIVTKIYVHVGDKVHTGSLILLLDVRNHT
NAFTINDKKNVVPSPYIVTNNDTRKGAICNDIHHDAAIHATPLVRHMARTFGIDLSKIKGSGRKGRILKEDIQNYIKNIS
MYYTNCMSSMQSDQLLPILSWPKIDFSKFGDITTVMLSKIQKTSGANLQRNWIMLPHVTQFDEADITDLESFRKQQNIDI
EKKKINCKITLLVFVMKAVAKALEELPRFNSSLSQDGETLILKKYINIGIAVDTPKGLLVPVLHDVNRKGIILLSQELEE
LSKKARTGNQLTPANMQGGSFTISNLGGIGGTAFTPIVNVPEVAILGISKSFIKPVWTGKKFTPRLMLPLSLSYDHRVID
GADGARFMTLINKIIADTRLLSM
>Mature_422_residues
TIEINIPNIGEDELEVTEIMVKIGDNINANQPLIIIEGDKSSMEIPSSCSGIVTKIYVHVGDKVHTGSLILLLDVRNHTN
AFTINDKKNVVPSPYIVTNNDTRKGAICNDIHHDAAIHATPLVRHMARTFGIDLSKIKGSGRKGRILKEDIQNYIKNISM
YYTNCMSSMQSDQLLPILSWPKIDFSKFGDITTVMLSKIQKTSGANLQRNWIMLPHVTQFDEADITDLESFRKQQNIDIE
KKKINCKITLLVFVMKAVAKALEELPRFNSSLSQDGETLILKKYINIGIAVDTPKGLLVPVLHDVNRKGIILLSQELEEL
SKKARTGNQLTPANMQGGSFTISNLGGIGGTAFTPIVNVPEVAILGISKSFIKPVWTGKKFTPRLMLPLSLSYDHRVIDG
ADGARFMTLINKIIADTRLLSM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=426, Percent_Identity=32.1596244131455, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI31711992, Length=301, Percent_Identity=30.8970099667774, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI19923748, Length=205, Percent_Identity=38.0487804878049, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI203098753, Length=444, Percent_Identity=27.2522522522523, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI203098816, Length=444, Percent_Identity=27.2522522522523, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI260898739, Length=151, Percent_Identity=37.7483443708609, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=56.7058823529412, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=28.1323877068558, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=408, Percent_Identity=27.2058823529412, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=218, Percent_Identity=37.1559633027523, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=418, Percent_Identity=28.2296650717703, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=322, Percent_Identity=28.8819875776398, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=420, Percent_Identity=26.9047619047619, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=425, Percent_Identity=25.1764705882353, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI18859875, Length=433, Percent_Identity=29.3302540415704, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=34.3891402714932, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129315, Length=208, Percent_Identity=33.1730769230769, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24582497, Length=208, Percent_Identity=33.1730769230769, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46888; Mature: 46757

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEINIPNIGEDELEVTEIMVKIGDNINANQPLIIIEGDKSSMEIPSSCSGIVTKIYVH
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEE
VGDKVHTGSLILLLDVRNHTNAFTINDKKNVVPSPYIVTNNDTRKGAICNDIHHDAAIHA
ECCEEECCCEEEEEEECCCCCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHCCCCHHHH
TPLVRHMARTFGIDLSKIKGSGRKGRILKEDIQNYIKNISMYYTNCMSSMQSDQLLPILS
HHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
WPKIDFSKFGDITTVMLSKIQKTSGANLQRNWIMLPHVTQFDEADITDLESFRKQQNIDI
CCCCCHHHHCCHHHHHHHHHHHCCCCCCEECEEEECCCCCCCCCCHHHHHHHHHHCCCCE
EKKKINCKITLLVFVMKAVAKALEELPRFNSSLSQDGETLILKKYINIGIAVDTPKGLLV
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEECEEEEEEECCCCCEE
PVLHDVNRKGIILLSQELEELSKKARTGNQLTPANMQGGSFTISNLGGIGGTAFTPIVNV
HHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHCCCCCC
PEVAILGISKSFIKPVWTGKKFTPRLMLPLSLSYDHRVIDGADGARFMTLINKIIADTRL
CCEEEEECCHHHHCHHCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHH
LSM
HCC
>Mature Secondary Structure 
TIEINIPNIGEDELEVTEIMVKIGDNINANQPLIIIEGDKSSMEIPSSCSGIVTKIYVH
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEE
VGDKVHTGSLILLLDVRNHTNAFTINDKKNVVPSPYIVTNNDTRKGAICNDIHHDAAIHA
ECCEEECCCEEEEEEECCCCCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHCCCCHHHH
TPLVRHMARTFGIDLSKIKGSGRKGRILKEDIQNYIKNISMYYTNCMSSMQSDQLLPILS
HHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
WPKIDFSKFGDITTVMLSKIQKTSGANLQRNWIMLPHVTQFDEADITDLESFRKQQNIDI
CCCCCHHHHCCHHHHHHHHHHHCCCCCCEECEEEECCCCCCCCCCHHHHHHHHHHCCCCE
EKKKINCKITLLVFVMKAVAKALEELPRFNSSLSQDGETLILKKYINIGIAVDTPKGLLV
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEECEEEEEEECCCCCEE
PVLHDVNRKGIILLSQELEELSKKARTGNQLTPANMQGGSFTISNLGGIGGTAFTPIVNV
HHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHCCCCCC
PEVAILGISKSFIKPVWTGKKFTPRLMLPLSLSYDHRVIDGADGARFMTLINKIIADTRL
CCEEEEECCHHHHCHHCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHH
LSM
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]