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The map label for this gene is lpdA [H]

Identifier: 71891939

GI number: 71891939

Start: 193981

End: 195405

Strand: Reverse

Name: lpdA [H]

Synonym: BPEN_156

Alternate gene names: 71891939

Gene position: 195405-193981 (Counterclockwise)

Preceding gene: 71891940

Following gene: 71891936

Centisome position: 24.68

GC content: 32.21

Gene sequence:

>1425_bases
ATGTATATAAAAATAAAAACTCAAATTCTTGTTTTAGGCGCGGGACCAGGAGGTTATTCCGCGGCTTTTCGCTGCGCTGA
TTTAGGTATGGATACTACAATAGTAGAACGTTATCCTGATTTAGGTGGAGTGTGTCTTAATGTTGGTTGTATTCCGTCTA
AAACATTATTACATATTGCCAAATTAATTGAAACACAAAAAAAATTCAATAAATATGGTCTCCTGTCAGGAGAAACACAT
ATCGATCTTAATAAGACGCGCTCTTGGAAAGATAAAATTATTGATCAATTGTCAAATAGTTTGAGTGCTATGGCAAAAAA
ACGGAACGTTAAAGTAATTGATGGGGTTGGTAAATTTATTGATAGTCATACTATTCAAGTTGAAAACAATGAAATGACCT
TAGAAATAGCATTCAATTACGCTATTGTAGCAGCAGGATCACATACTGTATCACTACCATGTATTCCTAATGATCAGCGA
ATTTGGAATTCAACTGATGCTCTATCTTTACAATCAATACCCGAACGTTTACTAATTATAGGCTCAGGAGCTATAGGATT
AGAAATGGCCACAATATATCGTGCTTTTGGATCAGAAATAGATATAGTAGAAATGTGTAATCGAATCATGCCAATTTTAG
ATGAAGATATTACTAATATTTTTACTAAAATAATTACTAAAAATATTAATTTAATTTTAAATACTAAAGTTAACATAGTA
GAAGCTAAGAAAGATGGTATTTATGTAACTATGGAAAATAAACAAACTTTATTAAAAAATACTCAACGTTATGATGCATT
ATTAGTAGCAATTGGGCGCGCTCCAAATGGTAACATGTTGAATATCGAGCGTGCGGGAGTAAATGTAAATGAATATGGTT
TCATTCCTGTAGATAAGCAGATGCGTACTAATGTTCAACACATTTTTGCTATTGGAGATATTATTGGATATCCAATGCTA
GCTCATAAAAGTATTCATGAAGGACATGTAGCTGCAGAAGTTATTTCCGGTAAAAAACGTTGTTTTGATCCAATAATAAT
TCCATCCATTATATATACTGATCCAGAAATAGCGTGGGTTGGATATACTGAAAAAAGTGCTCAAGAAAAAAATATAGATT
ATGAAGTTGCACTTTTCCCTTGGATGGCTTCAGGTCGAGCAATTACTGAAGATTGTAAAGAAGGCATAACCAAATTAATT
TTTAATAAAAAAACGAACAGAATTATTGGGGGATCAATATTAGGCGCGAATGCTAGTGAAATATTAGGAGAAATTGCATT
AGCCATTGAAATGGGATGTGATGTAGAAGATATTACTCTAACCATTCATGCCCATCCAACCTTATATGAATCAATAGCAT
TAGCAGCATCTATTCATAATGGATCAATTACTGATTTGCCTAATATAAAAAAACAAACACAATAA

Upstream 100 bases:

>100_bases
TCCGTACCATATATAAGTTCTATACTGTAGAAATACATATTTCTATCAAACTAGATACTATAATTAATAGTTTTAATCAT
AAAATGAAGGGCACCATATT

Downstream 100 bases:

>100_bases
ATTTCACATGATTCTTTAAAATTTTAAATAAATAAATTAACTTACAAATAATACTTTATCGTTGTATAGTCTATTGCAAT
TCATAAGGTAATTTATAAGG

Product: dihydrolipoamide dehydrogenase, FAD/NAD(P)-binding, E3 component of the 2-oxoglutarate dehydrogenase and the pyruvate dehydrogenase complexes

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MYIKIKTQILVLGAGPGGYSAAFRCADLGMDTTIVERYPDLGGVCLNVGCIPSKTLLHIAKLIETQKKFNKYGLLSGETH
IDLNKTRSWKDKIIDQLSNSLSAMAKKRNVKVIDGVGKFIDSHTIQVENNEMTLEIAFNYAIVAAGSHTVSLPCIPNDQR
IWNSTDALSLQSIPERLLIIGSGAIGLEMATIYRAFGSEIDIVEMCNRIMPILDEDITNIFTKIITKNINLILNTKVNIV
EAKKDGIYVTMENKQTLLKNTQRYDALLVAIGRAPNGNMLNIERAGVNVNEYGFIPVDKQMRTNVQHIFAIGDIIGYPML
AHKSIHEGHVAAEVISGKKRCFDPIIIPSIIYTDPEIAWVGYTEKSAQEKNIDYEVALFPWMASGRAITEDCKEGITKLI
FNKKTNRIIGGSILGANASEILGEIALAIEMGCDVEDITLTIHAHPTLYESIALAASIHNGSITDLPNIKKQTQ

Sequences:

>Translated_474_residues
MYIKIKTQILVLGAGPGGYSAAFRCADLGMDTTIVERYPDLGGVCLNVGCIPSKTLLHIAKLIETQKKFNKYGLLSGETH
IDLNKTRSWKDKIIDQLSNSLSAMAKKRNVKVIDGVGKFIDSHTIQVENNEMTLEIAFNYAIVAAGSHTVSLPCIPNDQR
IWNSTDALSLQSIPERLLIIGSGAIGLEMATIYRAFGSEIDIVEMCNRIMPILDEDITNIFTKIITKNINLILNTKVNIV
EAKKDGIYVTMENKQTLLKNTQRYDALLVAIGRAPNGNMLNIERAGVNVNEYGFIPVDKQMRTNVQHIFAIGDIIGYPML
AHKSIHEGHVAAEVISGKKRCFDPIIIPSIIYTDPEIAWVGYTEKSAQEKNIDYEVALFPWMASGRAITEDCKEGITKLI
FNKKTNRIIGGSILGANASEILGEIALAIEMGCDVEDITLTIHAHPTLYESIALAASIHNGSITDLPNIKKQTQ
>Mature_474_residues
MYIKIKTQILVLGAGPGGYSAAFRCADLGMDTTIVERYPDLGGVCLNVGCIPSKTLLHIAKLIETQKKFNKYGLLSGETH
IDLNKTRSWKDKIIDQLSNSLSAMAKKRNVKVIDGVGKFIDSHTIQVENNEMTLEIAFNYAIVAAGSHTVSLPCIPNDQR
IWNSTDALSLQSIPERLLIIGSGAIGLEMATIYRAFGSEIDIVEMCNRIMPILDEDITNIFTKIITKNINLILNTKVNIV
EAKKDGIYVTMENKQTLLKNTQRYDALLVAIGRAPNGNMLNIERAGVNVNEYGFIPVDKQMRTNVQHIFAIGDIIGYPML
AHKSIHEGHVAAEVISGKKRCFDPIIIPSIIYTDPEIAWVGYTEKSAQEKNIDYEVALFPWMASGRAITEDCKEGITKLI
FNKKTNRIIGGSILGANASEILGEIALAIEMGCDVEDITLTIHAHPTLYESIALAASIHNGSITDLPNIKKQTQ

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=464, Percent_Identity=36.6379310344828, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=26.7108167770419, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=28.5057471264368, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=28.5057471264368, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=28.5057471264368, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=28.5057471264368, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=28.5057471264368, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=443, Percent_Identity=26.6365688487585, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI22035672, Length=435, Percent_Identity=25.9770114942529, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI291045268, Length=435, Percent_Identity=24.5977011494253, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=62.5263157894737, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=30.3964757709251, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=28.1385281385281, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=27.7522935779816, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=449, Percent_Identity=35.8574610244989, Blast_Score=298, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=26.0869565217391, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=25.8503401360544, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=25.8503401360544, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=439, Percent_Identity=23.9179954441913, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=462, Percent_Identity=36.7965367965368, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=30.2521008403361, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6325166, Length=449, Percent_Identity=26.0579064587973, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=35.6673960612691, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=26.9874476987448, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=26.3917525773196, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=26.3917525773196, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=26.3917525773196, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 52170; Mature: 52170

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00032 ANTENNAPEDIA ; PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIKIKTQILVLGAGPGGYSAAFRCADLGMDTTIVERYPDLGGVCLNVGCIPSKTLLHIA
CEEEEEEEEEEEECCCCCCHHHEEEHHCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHH
KLIETQKKFNKYGLLSGETHIDLNKTRSWKDKIIDQLSNSLSAMAKKRNVKVIDGVGKFI
HHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
DSHTIQVENNEMTLEIAFNYAIVAAGSHTVSLPCIPNDQRIWNSTDALSLQSIPERLLII
CCCEEEEECCEEEEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHCCCEEEEE
GSGAIGLEMATIYRAFGSEIDIVEMCNRIMPILDEDITNIFTKIITKNINLILNTKVNIV
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
EAKKDGIYVTMENKQTLLKNTQRYDALLVAIGRAPNGNMLNIERAGVNVNEYGFIPVDKQ
EECCCCEEEEECCCHHHHHCCHHHEEEEEEEECCCCCCEEEEEECCCCCCCCEEEECCHH
MRTNVQHIFAIGDIIGYPMLAHKSIHEGHVAAEVISGKKRCFDPIIIPSIIYTDPEIAWV
HHCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHCCHHHHCCCEEECEEEECCCCEEEE
GYTEKSAQEKNIDYEVALFPWMASGRAITEDCKEGITKLIFNKKTNRIIGGSILGANASE
ECCCCCCHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECCCHHH
ILGEIALAIEMGCDVEDITLTIHAHPTLYESIALAASIHNGSITDLPNIKKQTQ
HHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYIKIKTQILVLGAGPGGYSAAFRCADLGMDTTIVERYPDLGGVCLNVGCIPSKTLLHIA
CEEEEEEEEEEEECCCCCCHHHEEEHHCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHH
KLIETQKKFNKYGLLSGETHIDLNKTRSWKDKIIDQLSNSLSAMAKKRNVKVIDGVGKFI
HHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
DSHTIQVENNEMTLEIAFNYAIVAAGSHTVSLPCIPNDQRIWNSTDALSLQSIPERLLII
CCCEEEEECCEEEEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHCCCEEEEE
GSGAIGLEMATIYRAFGSEIDIVEMCNRIMPILDEDITNIFTKIITKNINLILNTKVNIV
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
EAKKDGIYVTMENKQTLLKNTQRYDALLVAIGRAPNGNMLNIERAGVNVNEYGFIPVDKQ
EECCCCEEEEECCCHHHHHCCHHHEEEEEEEECCCCCCEEEEEECCCCCCCCEEEECCHH
MRTNVQHIFAIGDIIGYPMLAHKSIHEGHVAAEVISGKKRCFDPIIIPSIIYTDPEIAWV
HHCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHCCHHHHCCCEEECEEEECCCCEEEE
GYTEKSAQEKNIDYEVALFPWMASGRAITEDCKEGITKLIFNKKTNRIIGGSILGANASE
ECCCCCCHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECCCHHH
ILGEIALAIEMGCDVEDITLTIHAHPTLYESIALAASIHNGSITDLPNIKKQTQ
HHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]