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The map label for this gene is murE [H]

Identifier: 71891924

GI number: 71891924

Start: 172499

End: 174010

Strand: Direct

Name: murE [H]

Synonym: BPEN_141

Alternate gene names: 71891924

Gene position: 172499-174010 (Clockwise)

Preceding gene: 71891923

Following gene: 71891925

Centisome position: 21.79

GC content: 32.61

Gene sequence:

>1512_bases
ATGTATGATTCACGTAATTTATATAAATTATTTATTCCATATGTTTTTAATAATCCTACTAGTCCTGCATTGACGGGGAT
GGAATTGGATAGTCGTAACATTGTATTGGGAAATTTATTTGTAGCAGTTAAAGGTTCTAAGACTGATGGAAGATTATATA
TTGACTATGCGATCAAAAAGGGAGCTTCGGCAGTTTTATTAGAATCTTGGAGCGATATAACAATATCTAAAAAATATAGT
TATCATTTAAATAAAATACCTGTGGTCCATGTTAACTATTTACCACGGTATTTGTCCGATATAGCAGGTAGTTTTTATAA
TCATCCGTCACGTTTTTTGGATTTAATTGGTGTTACTGGCACCAATGGGAAAACTACTATAACGCATTTGTTAGCTCGTT
GGGTCCAATTATTAGGAAAAAAAAGCGCTGTGATGGGTACTTTAGGTAACGGAATGTTAAATAATATGTGTTTGTCTAAT
AATACAACATGCTCTTCCATTGATGCTCAAAAAATATTATCTCAGTTTGTTAAAGATAAGATTACGTTTGTTGCTATGGA
GGTGTCATCACATGGATTAAATCAATATCGCGTGGATGCTTTATATTTTAAAGCAGCTATATTTACTAATTTAAGCCATG
ATCACTTAGATTATCATGGTAACGTTATGCAATACTCTATGTCTAAGTGGCGGTTATTTGATGAATTATGTGTAGAAAAA
TATATAATTAATGCTGATGATCCCGTTGGTTGTAGATGGTTATATTATTTGCCTCAAGCGATCGCAGTGACAATAACAAG
GAATTTACCATTTTTTTGGAAAGGTAAATGGATATGTGCGCTTAAGGTCAATTATCATATTCATGGCACGGATATAACTT
TTGATTCAAGTTGGGGGCATGGAGTAATTCATAGTCAATTATTAGGAGAATTCAACGTTAGTAATTTGTTATTAGCTTTA
GGTACGTTATTAATATTAGGATATCCTCTATCATTATTGGTACACACATCATCTCAATTACAACCGGTAGCTGGTAGAAT
GGAAGTATTTCGTTCTCATGGATATCCGACGGTTATAGTAGATTATGCACATACCCCGGATGCGTTAGAAAAAGTTCTAA
TTTCAATTAGACAATTTTATTATTGGGGTCAACTATGGTGTGTTTTTGGTTGCGGAGGAGATAGAGATCCAAGTAAACGT
GCTTTAATGGGTTGTATTGTAAGCCGGTATGCTGATTATATAATTATTACCAATGATAATCCACGCACGGAAGAACCACA
ATCGATTATAAATGATATTACTCGTGGTATAACACACCGAAAAAAAATCATCAAAATTATTCAAAATCGTTGCTGTGCGA
TACAGACTGCTATTTTAAAGGCATCTTTAGAAGATGTGATATTAATTTCTGGAAAAGGTCATGAAAAATACCAAATTATC
GGAAATAATCGCATATATCATTCTGATAAAGACATAGTAGAAAATTTTTTAAAGAACGATGTTATTTTATGA

Upstream 100 bases:

>100_bases
TTTTTAGCGCAGTGATGGGCAATGCATTAAAAATCATGAATGTAGTGCCAGATTTTTTGCAGTAATTTTATAAGCAATCA
GGTCTCTTTGAGAAAATTAT

Downstream 100 bases:

>100_bases
TTCCATTTAGTTTGCATGAAATAGCGCCAGTTTTAAACGCTAAATACATTGGTATGGATTTGATTATTCATGAGATTACT
ATTAATTCAAATATTATTCA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MYDSRNLYKLFIPYVFNNPTSPALTGMELDSRNIVLGNLFVAVKGSKTDGRLYIDYAIKKGASAVLLESWSDITISKKYS
YHLNKIPVVHVNYLPRYLSDIAGSFYNHPSRFLDLIGVTGTNGKTTITHLLARWVQLLGKKSAVMGTLGNGMLNNMCLSN
NTTCSSIDAQKILSQFVKDKITFVAMEVSSHGLNQYRVDALYFKAAIFTNLSHDHLDYHGNVMQYSMSKWRLFDELCVEK
YIINADDPVGCRWLYYLPQAIAVTITRNLPFFWKGKWICALKVNYHIHGTDITFDSSWGHGVIHSQLLGEFNVSNLLLAL
GTLLILGYPLSLLVHTSSQLQPVAGRMEVFRSHGYPTVIVDYAHTPDALEKVLISIRQFYYWGQLWCVFGCGGDRDPSKR
ALMGCIVSRYADYIIITNDNPRTEEPQSIINDITRGITHRKKIIKIIQNRCCAIQTAILKASLEDVILISGKGHEKYQII
GNNRIYHSDKDIVENFLKNDVIL

Sequences:

>Translated_503_residues
MYDSRNLYKLFIPYVFNNPTSPALTGMELDSRNIVLGNLFVAVKGSKTDGRLYIDYAIKKGASAVLLESWSDITISKKYS
YHLNKIPVVHVNYLPRYLSDIAGSFYNHPSRFLDLIGVTGTNGKTTITHLLARWVQLLGKKSAVMGTLGNGMLNNMCLSN
NTTCSSIDAQKILSQFVKDKITFVAMEVSSHGLNQYRVDALYFKAAIFTNLSHDHLDYHGNVMQYSMSKWRLFDELCVEK
YIINADDPVGCRWLYYLPQAIAVTITRNLPFFWKGKWICALKVNYHIHGTDITFDSSWGHGVIHSQLLGEFNVSNLLLAL
GTLLILGYPLSLLVHTSSQLQPVAGRMEVFRSHGYPTVIVDYAHTPDALEKVLISIRQFYYWGQLWCVFGCGGDRDPSKR
ALMGCIVSRYADYIIITNDNPRTEEPQSIINDITRGITHRKKIIKIIQNRCCAIQTAILKASLEDVILISGKGHEKYQII
GNNRIYHSDKDIVENFLKNDVIL
>Mature_503_residues
MYDSRNLYKLFIPYVFNNPTSPALTGMELDSRNIVLGNLFVAVKGSKTDGRLYIDYAIKKGASAVLLESWSDITISKKYS
YHLNKIPVVHVNYLPRYLSDIAGSFYNHPSRFLDLIGVTGTNGKTTITHLLARWVQLLGKKSAVMGTLGNGMLNNMCLSN
NTTCSSIDAQKILSQFVKDKITFVAMEVSSHGLNQYRVDALYFKAAIFTNLSHDHLDYHGNVMQYSMSKWRLFDELCVEK
YIINADDPVGCRWLYYLPQAIAVTITRNLPFFWKGKWICALKVNYHIHGTDITFDSSWGHGVIHSQLLGEFNVSNLLLAL
GTLLILGYPLSLLVHTSSQLQPVAGRMEVFRSHGYPTVIVDYAHTPDALEKVLISIRQFYYWGQLWCVFGCGGDRDPSKR
ALMGCIVSRYADYIIITNDNPRTEEPQSIINDITRGITHRKKIIKIIQNRCCAIQTAILKASLEDVILISGKGHEKYQII
GNNRIYHSDKDIVENFLKNDVIL

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=493, Percent_Identity=52.9411764705882, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 56927; Mature: 56927

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDSRNLYKLFIPYVFNNPTSPALTGMELDSRNIVLGNLFVAVKGSKTDGRLYIDYAIKK
CCCCCCEEEEEEEEEECCCCCCCEECEEECCCCEEEEEEEEEEECCCCCCEEEEEEEECC
GASAVLLESWSDITISKKYSYHLNKIPVVHVNYLPRYLSDIAGSFYNHPSRFLDLIGVTG
CCCEEEEECCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHEECC
TNGKTTITHLLARWVQLLGKKSAVMGTLGNGMLNNMCLSNNTTCSSIDAQKILSQFVKDK
CCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCEEECCCCCCCCCCHHHHHHHHHHCC
ITFVAMEVSSHGLNQYRVDALYFKAAIFTNLSHDHLDYHGNVMQYSMSKWRLFDELCVEK
EEEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
YIINADDPVGCRWLYYLPQAIAVTITRNLPFFWKGKWICALKVNYHIHGTDITFDSSWGH
EEECCCCCCCEEHHHCCCCEEEEEEECCCCEEECCCEEEEEEEEEEEECCEEEEECCCCC
GVIHSQLLGEFNVSNLLLALGTLLILGYPLSLLVHTSSQLQPVAGRMEVFRSHGYPTVIV
CHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHCCCCCEEEE
DYAHTPDALEKVLISIRQFYYWGQLWCVFGCGGDRDPSKRALMGCIVSRYADYIIITNDN
ECCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCC
PRTEEPQSIINDITRGITHRKKIIKIIQNRCCAIQTAILKASLEDVILISGKGHEKYQII
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEE
GNNRIYHSDKDIVENFLKNDVIL
ECCEEEECCHHHHHHHHHCCCCC
>Mature Secondary Structure
MYDSRNLYKLFIPYVFNNPTSPALTGMELDSRNIVLGNLFVAVKGSKTDGRLYIDYAIKK
CCCCCCEEEEEEEEEECCCCCCCEECEEECCCCEEEEEEEEEEECCCCCCEEEEEEEECC
GASAVLLESWSDITISKKYSYHLNKIPVVHVNYLPRYLSDIAGSFYNHPSRFLDLIGVTG
CCCEEEEECCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHEECC
TNGKTTITHLLARWVQLLGKKSAVMGTLGNGMLNNMCLSNNTTCSSIDAQKILSQFVKDK
CCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCEEECCCCCCCCCCHHHHHHHHHHCC
ITFVAMEVSSHGLNQYRVDALYFKAAIFTNLSHDHLDYHGNVMQYSMSKWRLFDELCVEK
EEEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
YIINADDPVGCRWLYYLPQAIAVTITRNLPFFWKGKWICALKVNYHIHGTDITFDSSWGH
EEECCCCCCCEEHHHCCCCEEEEEEECCCCEEECCCEEEEEEEEEEEECCEEEEECCCCC
GVIHSQLLGEFNVSNLLLALGTLLILGYPLSLLVHTSSQLQPVAGRMEVFRSHGYPTVIV
CHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHCCCCCEEEE
DYAHTPDALEKVLISIRQFYYWGQLWCVFGCGGDRDPSKRALMGCIVSRYADYIIITNDN
ECCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCC
PRTEEPQSIINDITRGITHRKKIIKIIQNRCCAIQTAILKASLEDVILISGKGHEKYQII
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEE
GNNRIYHSDKDIVENFLKNDVIL
ECCEEEECCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12886019 [H]