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The map label for this gene is murF [H]

Identifier: 71891925

GI number: 71891925

Start: 174007

End: 175380

Strand: Direct

Name: murF [H]

Synonym: BPEN_142

Alternate gene names: 71891925

Gene position: 174007-175380 (Clockwise)

Preceding gene: 71891924

Following gene: 71891926

Centisome position: 21.98

GC content: 30.28

Gene sequence:

>1374_bases
ATGATTCCATTTAGTTTGCATGAAATAGCGCCAGTTTTAAACGCTAAATACATTGGTATGGATTTGATTATTCATGAGAT
TACTATTAATTCAAATATTATTCATAAACAATGTATGTTTATAGCATTAATAGGTAAACGTTTTGATGGTCATGATTTTA
CAAGTCAAGCAATTGCTGCTGGAGCTCAGGCATTGCTAGTAAATCATCATATATTATTAGATGTTCCTCAATTAATAGTT
CCTGATACTCGTTGTGCTTTAATAATGTTGGCACGTTGGGTGCGCCAAAAAGTCTCATCAAAAGTTATTGCTATAACTGG
ATCAAGCGGTAAAACATCAGTAAAAGAAATGACCACGTCTATATTAAAAGAGTGTGGACATACAATAGCCACACAAGGTA
ACTTTAATAATATGATTGGAGTGCCGATTACTTTATTACGTTTAACGAAAAAAAACAATTTTGCAATTATCGAATTAGGA
ACAAGTTCTCCTGGAGAACTTGTTCAATTAAGTAAGATAGTTGCTGCTAATGTAGCTTTAGTAAATAATGTTTACCCTGC
ACATCTATCAGGTTTTAAATCGTTATCTAAAATAGGAAAAGAAAAAGGGAAAATTTTTTCTGAATTAGATTTAAATGGAA
CAGGTATTATAAATGCTGATAGTCATGCTTTGTCTTTATGGCGTAAACCATTGAAGGGAAAATCTATGTGGTTTTTTTCT
TTACGTACTAACGTAGGCATAGATTTTTTTGCAAGTAATATTATTTCTGAAAAAAATAGAATACGGTTTATTTTACATAC
TCCATATGGAACTTCTCCAGTGGTTTTATCAATGTTAGGCACGCATAATGTGGCTAATGCTTTAGCTGCTAGCGCGCTTG
CATTTTCTGTAGGAGCAAATTTATCAGAAGTAGTTTATGGATTAGAAAATATAAAAACGTTACCTGGTAGATTATTTCCA
ATTATTTTAGGTGAAGGTAAATTATTATTAGATGATACTTATAATTCAAACGTTGGTTCTATGATATCTGCTATTCATGT
ACTAACTACAATGCCAGGTTATCGTATATTGGTGGTCAGTGATATGTTAGAATTAGGAAAATATAAATCAATCAAATATC
ATTGTTATATTGGGAAATTAATCGCAACGACAAATATCAATAAAATTCTTACCATAGGTAGTGTTAGTTGTTGCATTTCT
AAAATATGTGGAAAAGGAAAACATTTTCGAAATAAAAATAAATTAATTATTTATATCAAGCAGATGTTATCTATCCATAA
TTCAATTAGTGTATTAGTAAAAGGATCACGAGGCTTTAAAATGGAGCAAATAATAAATGCTATTCGAGATGAATCAAAAT
GTTATTTTGGCTAA

Upstream 100 bases:

>100_bases
CTGGAAAAGGTCATGAAAAATACCAAATTATCGGAAATAATCGCATATATCATTCTGATAAAGACATAGTAGAAAATTTT
TTAAAGAACGATGTTATTTT

Downstream 100 bases:

>100_bases
CAGAGAATGTATTAGCACTATATTCATCCAGATTTAATATTGTTTGTCATTTAACTTTCAGAGCTATTATTAGTTTTTTA
TCAGCATTGTTTATTTCTTT

Product: D-alanine:D-alanine-adding enzyme

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MIPFSLHEIAPVLNAKYIGMDLIIHEITINSNIIHKQCMFIALIGKRFDGHDFTSQAIAAGAQALLVNHHILLDVPQLIV
PDTRCALIMLARWVRQKVSSKVIAITGSSGKTSVKEMTTSILKECGHTIATQGNFNNMIGVPITLLRLTKKNNFAIIELG
TSSPGELVQLSKIVAANVALVNNVYPAHLSGFKSLSKIGKEKGKIFSELDLNGTGIINADSHALSLWRKPLKGKSMWFFS
LRTNVGIDFFASNIISEKNRIRFILHTPYGTSPVVLSMLGTHNVANALAASALAFSVGANLSEVVYGLENIKTLPGRLFP
IILGEGKLLLDDTYNSNVGSMISAIHVLTTMPGYRILVVSDMLELGKYKSIKYHCYIGKLIATTNINKILTIGSVSCCIS
KICGKGKHFRNKNKLIIYIKQMLSIHNSISVLVKGSRGFKMEQIINAIRDESKCYFG

Sequences:

>Translated_457_residues
MIPFSLHEIAPVLNAKYIGMDLIIHEITINSNIIHKQCMFIALIGKRFDGHDFTSQAIAAGAQALLVNHHILLDVPQLIV
PDTRCALIMLARWVRQKVSSKVIAITGSSGKTSVKEMTTSILKECGHTIATQGNFNNMIGVPITLLRLTKKNNFAIIELG
TSSPGELVQLSKIVAANVALVNNVYPAHLSGFKSLSKIGKEKGKIFSELDLNGTGIINADSHALSLWRKPLKGKSMWFFS
LRTNVGIDFFASNIISEKNRIRFILHTPYGTSPVVLSMLGTHNVANALAASALAFSVGANLSEVVYGLENIKTLPGRLFP
IILGEGKLLLDDTYNSNVGSMISAIHVLTTMPGYRILVVSDMLELGKYKSIKYHCYIGKLIATTNINKILTIGSVSCCIS
KICGKGKHFRNKNKLIIYIKQMLSIHNSISVLVKGSRGFKMEQIINAIRDESKCYFG
>Mature_457_residues
MIPFSLHEIAPVLNAKYIGMDLIIHEITINSNIIHKQCMFIALIGKRFDGHDFTSQAIAAGAQALLVNHHILLDVPQLIV
PDTRCALIMLARWVRQKVSSKVIAITGSSGKTSVKEMTTSILKECGHTIATQGNFNNMIGVPITLLRLTKKNNFAIIELG
TSSPGELVQLSKIVAANVALVNNVYPAHLSGFKSLSKIGKEKGKIFSELDLNGTGIINADSHALSLWRKPLKGKSMWFFS
LRTNVGIDFFASNIISEKNRIRFILHTPYGTSPVVLSMLGTHNVANALAASALAFSVGANLSEVVYGLENIKTLPGRLFP
IILGEGKLLLDDTYNSNVGSMISAIHVLTTMPGYRILVVSDMLELGKYKSIKYHCYIGKLIATTNINKILTIGSVSCCIS
KICGKGKHFRNKNKLIIYIKQMLSIHNSISVLVKGSRGFKMEQIINAIRDESKCYFG

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=454, Percent_Identity=46.9162995594714, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50036; Mature: 50036

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFSLHEIAPVLNAKYIGMDLIIHEITINSNIIHKQCMFIALIGKRFDGHDFTSQAIAA
CCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
GAQALLVNHHILLDVPQLIVPDTRCALIMLARWVRQKVSSKVIAITGSSGKTSVKEMTTS
CCHHHEEECEEEEECHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
ILKECGHTIATQGNFNNMIGVPITLLRLTKKNNFAIIELGTSSPGELVQLSKIVAANVAL
HHHHCCCEEECCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
VNNVYPAHLSGFKSLSKIGKEKGKIFSELDLNGTGIINADSHALSLWRKPLKGKSMWFFS
HCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCCCCCCCEEEEE
LRTNVGIDFFASNIISEKNRIRFILHTPYGTSPVVLSMLGTHNVANALAASALAFSVGAN
EECCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCC
LSEVVYGLENIKTLPGRLFPIILGEGKLLLDDTYNSNVGSMISAIHVLTTMPGYRILVVS
HHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEH
DMLELGKYKSIKYHCYIGKLIATTNINKILTIGSVSCCISKICGKGKHFRNKNKLIIYIK
HHHHHCCCCCEEEEEEEEEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCCEEEEHH
QMLSIHNSISVLVKGSRGFKMEQIINAIRDESKCYFG
HHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIPFSLHEIAPVLNAKYIGMDLIIHEITINSNIIHKQCMFIALIGKRFDGHDFTSQAIAA
CCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
GAQALLVNHHILLDVPQLIVPDTRCALIMLARWVRQKVSSKVIAITGSSGKTSVKEMTTS
CCHHHEEECEEEEECHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
ILKECGHTIATQGNFNNMIGVPITLLRLTKKNNFAIIELGTSSPGELVQLSKIVAANVAL
HHHHCCCEEECCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
VNNVYPAHLSGFKSLSKIGKEKGKIFSELDLNGTGIINADSHALSLWRKPLKGKSMWFFS
HCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCCCCCCCEEEEE
LRTNVGIDFFASNIISEKNRIRFILHTPYGTSPVVLSMLGTHNVANALAASALAFSVGAN
EECCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCC
LSEVVYGLENIKTLPGRLFPIILGEGKLLLDDTYNSNVGSMISAIHVLTTMPGYRILVVS
HHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEH
DMLELGKYKSIKYHCYIGKLIATTNINKILTIGSVSCCISKICGKGKHFRNKNKLIIYIK
HHHHHCCCCCEEEEEEEEEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCCEEEEHH
QMLSIHNSISVLVKGSRGFKMEQIINAIRDESKCYFG
HHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]