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The map label for this gene is leuA

Identifier: 71891920

GI number: 71891920

Start: 167418

End: 168971

Strand: Reverse

Name: leuA

Synonym: BPEN_137

Alternate gene names: 71891920

Gene position: 168971-167418 (Counterclockwise)

Preceding gene: 71891936

Following gene: 71891919

Centisome position: 21.34

GC content: 33.46

Gene sequence:

>1554_bases
ATGAGTCAACAAGTCATTATTTTCGATACAACTTTACGGGACGGTGAACAGGCTTTACAAGCGAGTTTAAACGTAAAGGA
AAAATTGCAGATTGCTTTTGCTTTAGAACGATTAGGGGTAGATATTATGGAAGTTGGATTTCCAATATCCTCTCCAGGAG
ATTTTGAATCGGTTCATACAATAGCTCAAAAAATAAAAAATAGTTTAGTATGCGCCTTGGCTAGATGTATTGATAACGAT
ATTGATGTTGCAGCTGAAGCGTTAAAAGTTGCAAAAAATTTTCGTATTCATATATTCTTACCCACTTCAAATGTACATAT
TCAATCTAAATTGAAAAAAAACTTTGATCAGATTATAGACATGACCGTTCATGCGATACGTTATGCAAGAAAATACACTG
ATGACATAGAGTTTTCTTGCGAAGATGCTGGACGCACCAATATCGATAATTTATGTCGTATTGTTGAAATTGCTATTCAA
TCCGGAGCAAGCACAATTAATATTCCTGACACAGTGGGCTACACCACTCCATATCAGTTTGGGCAAATTATTACTTCTTT
ATATAATCGTGTCCCTATTATTGATAAAGCTATCATTTCTGTTCATTGTCATGATGATTTAGGAATGGCAGTTGGTAATT
CTATTTCAGCTATACAGGCAGGAGCTCGTCAAATTGAAGGTACTATAAATGGTATTGGTGAACGAGCAGGAAATACTGCT
CTAGAAGAAATAATTATGGCCATCAAAGTACGAGAAGATTTACTGAACGTACATACTAATGTTCGTTGTCAAGAGATTTA
TAGAGCCAGTCAAGTAGTTAGTCAATTATGTAATATCCCTATCCCGGCTAATAAAGCAATAGTTGGCTCCAATGCTTTCT
CTCATTCTGCAGGTATTCATCAAGATGGTATATTGAAAAATAGAAAAAATTATGAAATTATGACTCCTGAAACTATTGGT
TTAAAAGATGTTAAATTAAATCTCACATCTCGATCTGGAAGAGCGGCAGTAAAACATCATATGAAAACAATGGGTTATCA
AGAAAGTGATTATGACATGGATAAATTATATGATGTTTTTTTAGAATTAGCAGATAAAAAAGGACAGGTATTTGATTATG
ATCTAGAAGCATTAGCATTCATTAACAATCAACAAGAACAATCAGAATTCTTCAGATTAAAATGTTTTCATGTAGATTCT
AGCTCTTCTGAAGTTGCTCACGCCTCAGTAAAATTATATTGTGGAAACAACACATACACTCATTCGTCGAGTGGTAATGG
GCCAATAGATGCAATTTATGAAGCGTTAACTCACATTTCTAAGTTATCTATAAATTTAGAAAGATATCAACTGAATGCAA
AAGGACATGGTCGTAACGCGTTAGGACAAGTGGATATTGTTGTATCGTATGAAGGACGCAATTTTCATGGAGTTGGATTA
GATACAGATGTAATAAAATCTTCAGTAAAAGCCATGATTCATGTTTTAAATAATATTTGGCGCGCGAAACAAGTGATAAT
TCAACGCAAATATATAAAAAAAAATAATAACTAG

Upstream 100 bases:

>100_bases
TTATTATTTAAAATCTGATATTATTGGTTCACAATTAGTATGTTATTAATTAAAATATTATATACATATCCATCCTAACA
TTCATGAGATGTAGATAATC

Downstream 100 bases:

>100_bases
TAATTTGGAAAATCTAAATACTTATGAATAATAATTATCATATTGCAGTATTACCAGGGGATGGTATCGGACCGGAAACT
ACAAGACAAGTTTATAAAAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSQQVIIFDTTLRDGEQALQASLNVKEKLQIAFALERLGVDIMEVGFPISSPGDFESVHTIAQKIKNSLVCALARCIDND
IDVAAEALKVAKNFRIHIFLPTSNVHIQSKLKKNFDQIIDMTVHAIRYARKYTDDIEFSCEDAGRTNIDNLCRIVEIAIQ
SGASTINIPDTVGYTTPYQFGQIITSLYNRVPIIDKAIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTA
LEEIIMAIKVREDLLNVHTNVRCQEIYRASQVVSQLCNIPIPANKAIVGSNAFSHSAGIHQDGILKNRKNYEIMTPETIG
LKDVKLNLTSRSGRAAVKHHMKTMGYQESDYDMDKLYDVFLELADKKGQVFDYDLEALAFINNQQEQSEFFRLKCFHVDS
SSSEVAHASVKLYCGNNTYTHSSSGNGPIDAIYEALTHISKLSINLERYQLNAKGHGRNALGQVDIVVSYEGRNFHGVGL
DTDVIKSSVKAMIHVLNNIWRAKQVIIQRKYIKKNNN

Sequences:

>Translated_517_residues
MSQQVIIFDTTLRDGEQALQASLNVKEKLQIAFALERLGVDIMEVGFPISSPGDFESVHTIAQKIKNSLVCALARCIDND
IDVAAEALKVAKNFRIHIFLPTSNVHIQSKLKKNFDQIIDMTVHAIRYARKYTDDIEFSCEDAGRTNIDNLCRIVEIAIQ
SGASTINIPDTVGYTTPYQFGQIITSLYNRVPIIDKAIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTA
LEEIIMAIKVREDLLNVHTNVRCQEIYRASQVVSQLCNIPIPANKAIVGSNAFSHSAGIHQDGILKNRKNYEIMTPETIG
LKDVKLNLTSRSGRAAVKHHMKTMGYQESDYDMDKLYDVFLELADKKGQVFDYDLEALAFINNQQEQSEFFRLKCFHVDS
SSSEVAHASVKLYCGNNTYTHSSSGNGPIDAIYEALTHISKLSINLERYQLNAKGHGRNALGQVDIVVSYEGRNFHGVGL
DTDVIKSSVKAMIHVLNNIWRAKQVIIQRKYIKKNNN
>Mature_516_residues
SQQVIIFDTTLRDGEQALQASLNVKEKLQIAFALERLGVDIMEVGFPISSPGDFESVHTIAQKIKNSLVCALARCIDNDI
DVAAEALKVAKNFRIHIFLPTSNVHIQSKLKKNFDQIIDMTVHAIRYARKYTDDIEFSCEDAGRTNIDNLCRIVEIAIQS
GASTINIPDTVGYTTPYQFGQIITSLYNRVPIIDKAIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTAL
EEIIMAIKVREDLLNVHTNVRCQEIYRASQVVSQLCNIPIPANKAIVGSNAFSHSAGIHQDGILKNRKNYEIMTPETIGL
KDVKLNLTSRSGRAAVKHHMKTMGYQESDYDMDKLYDVFLELADKKGQVFDYDLEALAFINNQQEQSEFFRLKCFHVDSS
SSEVAHASVKLYCGNNTYTHSSSGNGPIDAIYEALTHISKLSINLERYQLNAKGHGRNALGQVDIVVSYEGRNFHGVGLD
TDVIKSSVKAMIHVLNNIWRAKQVIIQRKYIKKNNN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=181, Percent_Identity=24.8618784530387, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786261, Length=519, Percent_Identity=68.2080924855491, Blast_Score=768, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320019, Length=402, Percent_Identity=27.8606965174129, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320071, Length=344, Percent_Identity=29.6511627906977, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=537, Percent_Identity=25.512104283054, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=544, Percent_Identity=24.4485294117647, Blast_Score=129, Evalue=8e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BLOPB (Q493R0)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277650.1
- ProteinModelPortal:   Q493R0
- SMR:   Q493R0
- STRING:   Q493R0
- GeneID:   3563105
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_137
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   CBLO291272:BPEN_137-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57500; Mature: 57369

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQVIIFDTTLRDGEQALQASLNVKEKLQIAFALERLGVDIMEVGFPISSPGDFESVHT
CCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAQKIKNSLVCALARCIDNDIDVAAEALKVAKNFRIHIFLPTSNVHIQSKLKKNFDQIID
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
MTVHAIRYARKYTDDIEFSCEDAGRTNIDNLCRIVEIAIQSGASTINIPDTVGYTTPYQF
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHH
GQIITSLYNRVPIIDKAIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTA
HHHHHHHHHCCCCEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
LEEIIMAIKVREDLLNVHTNVRCQEIYRASQVVSQLCNIPIPANKAIVGSNAFSHSAGIH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGILKNRKNYEIMTPETIGLKDVKLNLTSRSGRAAVKHHMKTMGYQESDYDMDKLYDVF
CCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LELADKKGQVFDYDLEALAFINNQQEQSEFFRLKCFHVDSSSSEVAHASVKLYCGNNTYT
HHHHCCCCCEEECCHHHHHHHCCCHHHHHEEEEEEEEECCCCCCEEEEEEEEEECCCEEE
HSSSGNGPIDAIYEALTHISKLSINLERYQLNAKGHGRNALGQVDIVVSYEGRNFHGVGL
CCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEECCCC
DTDVIKSSVKAMIHVLNNIWRAKQVIIQRKYIKKNNN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQQVIIFDTTLRDGEQALQASLNVKEKLQIAFALERLGVDIMEVGFPISSPGDFESVHT
CCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAQKIKNSLVCALARCIDNDIDVAAEALKVAKNFRIHIFLPTSNVHIQSKLKKNFDQIID
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
MTVHAIRYARKYTDDIEFSCEDAGRTNIDNLCRIVEIAIQSGASTINIPDTVGYTTPYQF
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHH
GQIITSLYNRVPIIDKAIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTA
HHHHHHHHHCCCCEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
LEEIIMAIKVREDLLNVHTNVRCQEIYRASQVVSQLCNIPIPANKAIVGSNAFSHSAGIH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGILKNRKNYEIMTPETIGLKDVKLNLTSRSGRAAVKHHMKTMGYQESDYDMDKLYDVF
CCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LELADKKGQVFDYDLEALAFINNQQEQSEFFRLKCFHVDSSSSEVAHASVKLYCGNNTYT
HHHHCCCCCEEECCHHHHHHHCCCHHHHHEEEEEEEEECCCCCCEEEEEEEEEECCCEEE
HSSSGNGPIDAIYEALTHISKLSINLERYQLNAKGHGRNALGQVDIVVSYEGRNFHGVGL
CCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEEEEECCCEEECCCC
DTDVIKSSVKAMIHVLNNIWRAKQVIIQRKYIKKNNN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA