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The map label for this gene is leuB

Identifier: 71891919

GI number: 71891919

Start: 166294

End: 167394

Strand: Reverse

Name: leuB

Synonym: BPEN_136

Alternate gene names: 71891919

Gene position: 167394-166294 (Counterclockwise)

Preceding gene: 71891920

Following gene: 71891918

Centisome position: 21.14

GC content: 31.97

Gene sequence:

>1101_bases
ATGAATAATAATTATCATATTGCAGTATTACCAGGGGATGGTATCGGACCGGAAACTACAAGACAAGTTTATAAAATATT
AAATGTAATAAAAAAACAATTTCAAATCAATATTGTCACCACAGAACATAAAATAGGAGGAGATGCAATTAATAGTGAAG
GCACTCCTTTTCCGAAAAGTACTTTAAAATATTGCGAACAATCAAATGCTATATTATTTGGAGCAGTAGGAGGACCACAG
TGGACACATTTAAAAGGATCAGAAAGCCCGGAACAAGGAGCTTTATTAGCTTTACGCAAACACTTTAATTTATTCGCTAA
TTTAAGACCTATATACCTTGCTGATGAATTAAAAGAATTATCTCCTTTAAATATAAATATGATTCCAAATGGAATTGACA
TTATATTCGTACGTGAATTAACCGGAGGTATTTATTTTGGCCAACCAAAAGGACGTTCAGGGACAGGTTTAAACGAATAT
GCTTTTGATACTGCAGTTTATCATCGCTTTGAAATAGAACGTATTGCCAACATTGCTTTTAAATTAGCTCAAAAAAGACG
TAAACGTGTGTCTTCTATAGACAAAGCTAACGTGCTACATACTTCTATGTTATGGAGAGAAGTGGTATCACATCTTGCAA
AAAATTATCCAGATGTTGAATTAGAACACTTATACATTGACAATGCTAGCATGCAATTAATAAACAATCCTTCTAAATTC
GATATTATTCTATGTCCAAATTTATTTGGGGACATTCTATCTGATGAATGTGCTATGATAAGTGGATCCATTGGAATGTT
ACCTTCTGCTAGTATTAATGAACACAATTTTGGTTTGTATGAACCTGCCGGAGGATCAGCTCCGGATATTGCAGAAAAAA
ATATCGCTAATCCTATCGCACATATTCTCTCTATTGCGTTATTATTTAGATATAGTTTAAAATTAGATCATATTGCAATT
AAAATTGAAAAAGCAGTTTCTCAAGCATTAATGTTAGGATATCGAACTATTGATATAGCAAAAAAACACGAAAAAAGCAT
AAGCACTAATGAAATGGGTGATATTATAGCTGCGTTAATTAAAAACGAAAGAGAAAAATAA

Upstream 100 bases:

>100_bases
TTCATGTTTTAAATAATATTTGGCGCGCGAAACAAGTGATAATTCAACGCAAATATATAAAAAAAAATAATAACTAGTAA
TTTGGAAAATCTAAATACTT

Downstream 100 bases:

>100_bases
TATGGGAAAATCCTTATACCAGAAGTTATACGATGCACATATAGTTCATAAGAATAAAGATGATGTTCCAATATTATATA
TTGATAGACATTTGTTGCAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNNNYHIAVLPGDGIGPETTRQVYKILNVIKKQFQINIVTTEHKIGGDAINSEGTPFPKSTLKYCEQSNAILFGAVGGPQ
WTHLKGSESPEQGALLALRKHFNLFANLRPIYLADELKELSPLNINMIPNGIDIIFVRELTGGIYFGQPKGRSGTGLNEY
AFDTAVYHRFEIERIANIAFKLAQKRRKRVSSIDKANVLHTSMLWREVVSHLAKNYPDVELEHLYIDNASMQLINNPSKF
DIILCPNLFGDILSDECAMISGSIGMLPSASINEHNFGLYEPAGGSAPDIAEKNIANPIAHILSIALLFRYSLKLDHIAI
KIEKAVSQALMLGYRTIDIAKKHEKSISTNEMGDIIAALIKNEREK

Sequences:

>Translated_366_residues
MNNNYHIAVLPGDGIGPETTRQVYKILNVIKKQFQINIVTTEHKIGGDAINSEGTPFPKSTLKYCEQSNAILFGAVGGPQ
WTHLKGSESPEQGALLALRKHFNLFANLRPIYLADELKELSPLNINMIPNGIDIIFVRELTGGIYFGQPKGRSGTGLNEY
AFDTAVYHRFEIERIANIAFKLAQKRRKRVSSIDKANVLHTSMLWREVVSHLAKNYPDVELEHLYIDNASMQLINNPSKF
DIILCPNLFGDILSDECAMISGSIGMLPSASINEHNFGLYEPAGGSAPDIAEKNIANPIAHILSIALLFRYSLKLDHIAI
KIEKAVSQALMLGYRTIDIAKKHEKSISTNEMGDIIAALIKNEREK
>Mature_366_residues
MNNNYHIAVLPGDGIGPETTRQVYKILNVIKKQFQINIVTTEHKIGGDAINSEGTPFPKSTLKYCEQSNAILFGAVGGPQ
WTHLKGSESPEQGALLALRKHFNLFANLRPIYLADELKELSPLNINMIPNGIDIIFVRELTGGIYFGQPKGRSGTGLNEY
AFDTAVYHRFEIERIANIAFKLAQKRRKRVSSIDKANVLHTSMLWREVVSHLAKNYPDVELEHLYIDNASMQLINNPSKF
DIILCPNLFGDILSDECAMISGSIGMLPSASINEHNFGLYEPAGGSAPDIAEKNIANPIAHILSIALLFRYSLKLDHIAI
KIEKAVSQALMLGYRTIDIAKKHEKSISTNEMGDIIAALIKNEREK

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=354, Percent_Identity=29.9435028248588, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI4758582, Length=198, Percent_Identity=35.8585858585859, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178838, Length=198, Percent_Identity=35.8585858585859, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178816, Length=321, Percent_Identity=26.4797507788162, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178821, Length=321, Percent_Identity=26.4797507788162, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=147, Percent_Identity=34.0136054421769, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081683, Length=360, Percent_Identity=63.0555555555556, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1788101, Length=370, Percent_Identity=32.972972972973, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1787381, Length=401, Percent_Identity=23.1920199501247, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71986051, Length=336, Percent_Identity=30.952380952381, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=29.0598290598291, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=314, Percent_Identity=28.9808917197452, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=366, Percent_Identity=27.0491803278689, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=372, Percent_Identity=42.4731182795699, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=34, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=30.0561797752809, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=350, Percent_Identity=31.1428571428571, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24643268, Length=356, Percent_Identity=32.3033707865169, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24643270, Length=356, Percent_Identity=32.3033707865169, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24661184, Length=366, Percent_Identity=30.327868852459, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161078633, Length=359, Percent_Identity=26.7409470752089, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650122, Length=359, Percent_Identity=26.7409470752089, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078637, Length=359, Percent_Identity=26.7409470752089, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078635, Length=359, Percent_Identity=26.7409470752089, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078639, Length=357, Percent_Identity=26.890756302521, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=25.4335260115607, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=25.4335260115607, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130355, Length=343, Percent_Identity=21.5743440233236, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_BLOPB (Q493R1)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277649.1
- ProteinModelPortal:   Q493R1
- SMR:   Q493R1
- STRING:   Q493R1
- GeneID:   3563104
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_136
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   CBLO291272:BPEN_136-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 40664; Mature: 40664

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 99-99 BINDING 109-109 BINDING 138-138 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNYHIAVLPGDGIGPETTRQVYKILNVIKKQFQINIVTTEHKIGGDAINSEGTPFPKS
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCHH
TLKYCEQSNAILFGAVGGPQWTHLKGSESPEQGALLALRKHFNLFANLRPIYLADELKEL
HHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHC
SPLNINMIPNGIDIIFVRELTGGIYFGQPKGRSGTGLNEYAFDTAVYHRFEIERIANIAF
CCCEEEECCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KLAQKRRKRVSSIDKANVLHTSMLWREVVSHLAKNYPDVELEHLYIDNASMQLINNPSKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCE
DIILCPNLFGDILSDECAMISGSIGMLPSASINEHNFGLYEPAGGSAPDIAEKNIANPIA
EEEECCHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHH
HILSIALLFRYSLKLDHIAIKIEKAVSQALMLGYRTIDIAKKHEKSISTNEMGDIIAALI
HHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KNEREK
HHCCCC
>Mature Secondary Structure
MNNNYHIAVLPGDGIGPETTRQVYKILNVIKKQFQINIVTTEHKIGGDAINSEGTPFPKS
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCHH
TLKYCEQSNAILFGAVGGPQWTHLKGSESPEQGALLALRKHFNLFANLRPIYLADELKEL
HHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHC
SPLNINMIPNGIDIIFVRELTGGIYFGQPKGRSGTGLNEYAFDTAVYHRFEIERIANIAF
CCCEEEECCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KLAQKRRKRVSSIDKANVLHTSMLWREVVSHLAKNYPDVELEHLYIDNASMQLINNPSKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCE
DIILCPNLFGDILSDECAMISGSIGMLPSASINEHNFGLYEPAGGSAPDIAEKNIANPIA
EEEECCHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHH
HILSIALLFRYSLKLDHIAIKIEKAVSQALMLGYRTIDIAKKHEKSISTNEMGDIIAALI
HHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KNEREK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA