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The map label for this gene is leuC

Identifier: 71891918

GI number: 71891918

Start: 164892

End: 166292

Strand: Reverse

Name: leuC

Synonym: BPEN_135

Alternate gene names: 71891918

Gene position: 166292-164892 (Counterclockwise)

Preceding gene: 71891919

Following gene: 71891917

Centisome position: 21.01

GC content: 36.12

Gene sequence:

>1401_bases
ATGGGAAAATCCTTATACCAGAAGTTATACGATGCACATATAGTTCATAAGAATAAAGATGATGTTCCAATATTATATAT
TGATAGACATTTGTTGCATGAGGTTACATCACCCCAAGCTTTTGAAGCACTACGTCTAAGAAAACGTATAGTTAGACAAC
CAAATAAAACATTTGCCACTATGGATCATAACGTCCCTACTACAACAAAAAATATTAATCATTCTGGAAGTATGGCTAAA
GAACAATTAGATAGATTAACAAAAAATTGTAAAGATTTTGGAATTACGTTATTTGGTTTAAATCATCCTTATCAAGGAAT
AGTTCATGTTTTAGCGCCAGAACAAGGTATCACATTACCCGGTATGACTATCGTGTGCGGAGATTCTCATACTGCTACTC
ATGGAGCTTTTGGTGCTTTAGCTTTTGGAATAGGAACATCAGAGATAGAACATGTATTAGCAACACAAACTTTACAACAA
ATTCGATATAAATCAATGCAAATTAACCTTTCCGGTATTATCGCTCCTCAAATTACAGCCAAAGATATAATTTTAGCAAT
TATTGGAAAAATTGGACATGGAGGAGGATCCGGTCATGTAGTAGAGTTTTGTGGACCTATTATCACTCTCTTAAGCATGG
AAGAACGCATGACCCTGTGTAATATGGCTATTGAAATGGGAGCAACTTCTGCTTTAATAGCGCCAGATAGAACTACTTAC
GATTATTTAAAAAACCGTAAATTTTCTCCAACCGGAAAGAATTGGGAACAAGCATTATTATATTGGAAAACTTTATATTC
CGACCATGATGCTCACTTCGATAAAAAATTTGATTTTGACGTGTCTAATGTTAATCCACAAGTAACCTGGGGAACTAATC
CTAGTCAAGTTATCTCTATTGACCAACCAATCCCATCTCTAGAATCTTTTATAGATCCTATAGAACGCAATTCTGCTGAA
CGTGCTTTAATTTACATGAAACTTCAATCCAATACTTATTTAACAGATGTTTCTATCGATAAAGTATTCATAGGGTCATG
CACTAACTCACGTATTGAAGATTTACGTGTAGCGGCACAAACAATTAAAGGACATCATATTTCCAAAAATACACAAGCAA
TTGTAGTACCCGGATCTAAGTTAGTAAAAAATCAAGCAGAAAAAGAAGGATTAAATAAGATCTTCATACAAGCAGGATTC
GAGTGGCGTTTACCTGGTTGTTCTATGTGTCTAGCTATGAATAATGACAGACTCGAACCAGGTGAGCGATGTGCTTCTAC
TAGTAACCGAAACTTTGAAGGTCGCCAAGGGCGTGGGAGTCGTACTCATTTAGTTAGTCCAGCAATGGCGGCCGCCGCCG
CCATTGCTGGACGCTTTGTAGATATTCGAAAATTTAAATGA

Upstream 100 bases:

>100_bases
CGAACTATTGATATAGCAAAAAAACACGAAAAAAGCATAAGCACTAATGAAATGGGTGATATTATAGCTGCGTTAATTAA
AAACGAAAGAGAAAAATAAT

Downstream 100 bases:

>100_bases
TATATATAGGACATATGCGTACATATGATTCAATTTATTCAACATACTGGTATAGTTTTACCACTTGATATTTCTGATAT
TGATACTGATACTATAATCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGKSLYQKLYDAHIVHKNKDDVPILYIDRHLLHEVTSPQAFEALRLRKRIVRQPNKTFATMDHNVPTTTKNINHSGSMAK
EQLDRLTKNCKDFGITLFGLNHPYQGIVHVLAPEQGITLPGMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLQQ
IRYKSMQINLSGIIAPQITAKDIILAIIGKIGHGGGSGHVVEFCGPIITLLSMEERMTLCNMAIEMGATSALIAPDRTTY
DYLKNRKFSPTGKNWEQALLYWKTLYSDHDAHFDKKFDFDVSNVNPQVTWGTNPSQVISIDQPIPSLESFIDPIERNSAE
RALIYMKLQSNTYLTDVSIDKVFIGSCTNSRIEDLRVAAQTIKGHHISKNTQAIVVPGSKLVKNQAEKEGLNKIFIQAGF
EWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAIAGRFVDIRKFK

Sequences:

>Translated_466_residues
MGKSLYQKLYDAHIVHKNKDDVPILYIDRHLLHEVTSPQAFEALRLRKRIVRQPNKTFATMDHNVPTTTKNINHSGSMAK
EQLDRLTKNCKDFGITLFGLNHPYQGIVHVLAPEQGITLPGMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLQQ
IRYKSMQINLSGIIAPQITAKDIILAIIGKIGHGGGSGHVVEFCGPIITLLSMEERMTLCNMAIEMGATSALIAPDRTTY
DYLKNRKFSPTGKNWEQALLYWKTLYSDHDAHFDKKFDFDVSNVNPQVTWGTNPSQVISIDQPIPSLESFIDPIERNSAE
RALIYMKLQSNTYLTDVSIDKVFIGSCTNSRIEDLRVAAQTIKGHHISKNTQAIVVPGSKLVKNQAEKEGLNKIFIQAGF
EWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAIAGRFVDIRKFK
>Mature_465_residues
GKSLYQKLYDAHIVHKNKDDVPILYIDRHLLHEVTSPQAFEALRLRKRIVRQPNKTFATMDHNVPTTTKNINHSGSMAKE
QLDRLTKNCKDFGITLFGLNHPYQGIVHVLAPEQGITLPGMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLQQI
RYKSMQINLSGIIAPQITAKDIILAIIGKIGHGGGSGHVVEFCGPIITLLSMEERMTLCNMAIEMGATSALIAPDRTTYD
YLKNRKFSPTGKNWEQALLYWKTLYSDHDAHFDKKFDFDVSNVNPQVTWGTNPSQVISIDQPIPSLESFIDPIERNSAER
ALIYMKLQSNTYLTDVSIDKVFIGSCTNSRIEDLRVAAQTIKGHHISKNTQAIVVPGSKLVKNQAEKEGLNKIFIQAGFE
WRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAIAGRFVDIRKFK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI8659555, Length=412, Percent_Identity=27.1844660194175, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4501867, Length=398, Percent_Identity=25.1256281407035, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=23.3595800524934, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=68.8841201716738, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI87081781, Length=359, Percent_Identity=25.6267409470752, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=25.3481894150418, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367097, Length=412, Percent_Identity=24.2718446601942, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=402, Percent_Identity=28.8557213930348, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=402, Percent_Identity=28.8557213930348, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=308, Percent_Identity=30.1948051948052, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=457, Percent_Identity=25.164113785558, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=56.2632696390658, Blast_Score=534, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6320440, Length=426, Percent_Identity=27.4647887323944, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=397, Percent_Identity=28.463476070529, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=400, Percent_Identity=26.75, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI281365315, Length=398, Percent_Identity=27.3869346733668, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17864292, Length=398, Percent_Identity=27.3869346733668, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161076999, Length=399, Percent_Identity=27.3182957393484, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28571643, Length=399, Percent_Identity=27.0676691729323, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24645686, Length=374, Percent_Identity=29.144385026738, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137564, Length=374, Percent_Identity=27.807486631016, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_BLOPB (Q493R2)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277648.1
- STRING:   Q493R2
- GeneID:   3563103
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_135
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   CBLO291272:BPEN_135-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51699; Mature: 51568

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLYQKLYDAHIVHKNKDDVPILYIDRHLLHEVTSPQAFEALRLRKRIVRQPNKTFAT
CCHHHHHHHHHHHEEECCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
MDHNVPTTTKNINHSGSMAKEQLDRLTKNCKDFGITLFGLNHPYQGIVHVLAPEQGITLP
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLQQIRYKSMQINLSGIIAPQITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCH
KDIILAIIGKIGHGGGSGHVVEFCGPIITLLSMEERMTLCNMAIEMGATSALIAPDRTTY
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
DYLKNRKFSPTGKNWEQALLYWKTLYSDHDAHFDKKFDFDVSNVNPQVTWGTNPSQVISI
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEECCCCCEEEEE
DQPIPSLESFIDPIERNSAERALIYMKLQSNTYLTDVSIDKVFIGSCTNSRIEDLRVAAQ
CCCCCHHHHHHCHHHCCCCCEEEEEEEECCCEEEEEEEECEEEEECCCCCHHHHHHHHHH
TIKGHHISKNTQAIVVPGSKLVKNQAEKEGLNKIFIQAGFEWRLPGCSMCLAMNNDRLEP
HHCCCEECCCCCEEEECCHHHHHHHHHHHCCCEEEEEECCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAIAGRFVDIRKFK
HHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEECCC
>Mature Secondary Structure 
GKSLYQKLYDAHIVHKNKDDVPILYIDRHLLHEVTSPQAFEALRLRKRIVRQPNKTFAT
CHHHHHHHHHHHEEECCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
MDHNVPTTTKNINHSGSMAKEQLDRLTKNCKDFGITLFGLNHPYQGIVHVLAPEQGITLP
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEIEHVLATQTLQQIRYKSMQINLSGIIAPQITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCH
KDIILAIIGKIGHGGGSGHVVEFCGPIITLLSMEERMTLCNMAIEMGATSALIAPDRTTY
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
DYLKNRKFSPTGKNWEQALLYWKTLYSDHDAHFDKKFDFDVSNVNPQVTWGTNPSQVISI
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEECCCCCEEEEE
DQPIPSLESFIDPIERNSAERALIYMKLQSNTYLTDVSIDKVFIGSCTNSRIEDLRVAAQ
CCCCCHHHHHHCHHHCCCCCEEEEEEEECCCEEEEEEEECEEEEECCCCCHHHHHHHHHH
TIKGHHISKNTQAIVVPGSKLVKNQAEKEGLNKIFIQAGFEWRLPGCSMCLAMNNDRLEP
HHCCCEECCCCCEEEECCHHHHHHHHHHHCCCEEEEEECCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAIAGRFVDIRKFK
HHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA