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The map label for this gene is leuD

Identifier: 71891917

GI number: 71891917

Start: 164259

End: 164867

Strand: Reverse

Name: leuD

Synonym: BPEN_134

Alternate gene names: 71891917

Gene position: 164867-164259 (Counterclockwise)

Preceding gene: 71891918

Following gene: 71891916

Centisome position: 20.83

GC content: 25.94

Gene sequence:

>609_bases
ATGATTCAATTTATTCAACATACTGGTATAGTTTTACCACTTGATATTTCTGATATTGATACTGATACTATAATCCCAAA
ACAATTTTTAAAAGCTACTACTCGCTCTAATTTTGGGTTATACTTGTTTTTTAATTGGCGCTTTCTTGAAAATACACCTA
AAATCTTAAATCCAAATTTCGTATTAAATGATCCGTATTATAAACATGCTAGCATTTTGCTAACCCAAAGAAATTTTGGA
TGTGGATCATCTCGCGAACATGCAGTATGGGCACTCATGGATTATGGATTCAAAGTTATCATAGCTCCAAGTTTTGCAGA
TATTTTTCATAAAAATAGTTTTAATAATCGCTTATTGCTTATAACTTTATCAGAATTAAAAATAAATGATTTATTTAAAG
AAATTGCAACACAAAAAAAAGGTATATCTTTTACTGTAAATTTACACGAACAAATTATTTATGTACATAATAAAAAAAAG
TGTTTTTTTGAAATAAATGACTTCCATAAACGTTGTATGCTTAATAATATAGACAATATTAGCTTAACTTTGCAATACGA
TGTTGCTATTAAAAAATATGAAAATAACCAACCATCATATTTAAAATAA

Upstream 100 bases:

>100_bases
TCATTTAGTTAGTCCAGCAATGGCGGCCGCCGCCGCCATTGCTGGACGCTTTGTAGATATTCGAAAATTTAAATGATATA
TATAGGACATATGCGTACAT

Downstream 100 bases:

>100_bases
ATTGCAATAATAAAAATACGAACGCATTGGAACGCATATATATGAAAAAATTTCACAATAGTATGGATTAAAATAATTAA
AAATTTGTATCAAAATGACC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MIQFIQHTGIVLPLDISDIDTDTIIPKQFLKATTRSNFGLYLFFNWRFLENTPKILNPNFVLNDPYYKHASILLTQRNFG
CGSSREHAVWALMDYGFKVIIAPSFADIFHKNSFNNRLLLITLSELKINDLFKEIATQKKGISFTVNLHEQIIYVHNKKK
CFFEINDFHKRCMLNNIDNISLTLQYDVAIKKYENNQPSYLK

Sequences:

>Translated_202_residues
MIQFIQHTGIVLPLDISDIDTDTIIPKQFLKATTRSNFGLYLFFNWRFLENTPKILNPNFVLNDPYYKHASILLTQRNFG
CGSSREHAVWALMDYGFKVIIAPSFADIFHKNSFNNRLLLITLSELKINDLFKEIATQKKGISFTVNLHEQIIYVHNKKK
CFFEINDFHKRCMLNNIDNISLTLQYDVAIKKYENNQPSYLK
>Mature_202_residues
MIQFIQHTGIVLPLDISDIDTDTIIPKQFLKATTRSNFGLYLFFNWRFLENTPKILNPNFVLNDPYYKHASILLTQRNFG
CGSSREHAVWALMDYGFKVIIAPSFADIFHKNSFNNRLLLITLSELKINDLFKEIATQKKGISFTVNLHEQIIYVHNKKK
CFFEINDFHKRCMLNNIDNISLTLQYDVAIKKYENNQPSYLK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=199, Percent_Identity=54.2713567839196, Blast_Score=229, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=45.959595959596, Blast_Score=156, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_BLOPB (Q493R3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277647.1
- STRING:   Q493R3
- GeneID:   3563102
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_134
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   CBLO291272:BPEN_134-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23674; Mature: 23674

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFIQHTGIVLPLDISDIDTDTIIPKQFLKATTRSNFGLYLFFNWRFLENTPKILNPNF
CEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCEECCCE
VLNDPYYKHASILLTQRNFGCGSSREHAVWALMDYGFKVIIAPSFADIFHKNSFNNRLLL
EECCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHCCCCCCEEEE
ITLSELKINDLFKEIATQKKGISFTVNLHEQIIYVHNKKKCFFEINDFHKRCMLNNIDNI
EEECCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHCCCCCE
SLTLQYDVAIKKYENNQPSYLK
EEEEEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MIQFIQHTGIVLPLDISDIDTDTIIPKQFLKATTRSNFGLYLFFNWRFLENTPKILNPNF
CEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCEECCCE
VLNDPYYKHASILLTQRNFGCGSSREHAVWALMDYGFKVIIAPSFADIFHKNSFNNRLLL
EECCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHCCCCCCEEEE
ITLSELKINDLFKEIATQKKGISFTVNLHEQIIYVHNKKKCFFEINDFHKRCMLNNIDNI
EEECCCCHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHCCCCCE
SLTLQYDVAIKKYENNQPSYLK
EEEEEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA