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The map label for this gene is imp [H]

Identifier: 71891916

GI number: 71891916

Start: 161321

End: 163669

Strand: Reverse

Name: imp [H]

Synonym: BPEN_133

Alternate gene names: 71891916

Gene position: 163669-161321 (Counterclockwise)

Preceding gene: 71891917

Following gene: 71891915

Centisome position: 20.67

GC content: 26.61

Gene sequence:

>2349_bases
ATGATTATTAAGTTTTTTTTTAAAAACACTATCATTAATTCAATTTTTATAATATTTTATACTCAATTTTATTTTTTGGC
TCATGCATCTAATTGTCTGCAAATTCCTGTAGCCAATAATCTCTTTTCTATTGATCAACCCATCTATGTGTCATCTGATG
AAATAGATATTTATTATCCTAATAAAATTCAATTTTCTGGAGATGTCAATATGAAACAAGATAATAACATTTTCATGTCC
GACAAATTAATAATATCACTTAATGAAAAAAATGAATTATTATACAATACGCTGTATGCATGTGGCCATGTTAGCTATAA
CAACAACTATATTATATTAACTGGATCCCATGCTTGGATTAATTTTTATAATAAAAATATAGATATTCATGAAGGTACAT
ATCATTTCTCGGAACCTCCTATTCATGGAACAGCAAATTCTATAATGCAGAGAGGGAAAAATCGTTATACTATTGTCAAG
CAAGGAAAGTGTACTTCTTGTGTTTCAAACAAAAATTATTGGAACATAACAGGATCAGAAATGCTTTATGATCATCATAA
ACATAACATAGATGTATGGAATGCTTGTCTTAAAATAAAAAAAATACCAATATTCTATAGCCCTTATTTATCACTATCTT
TACATCAAAATAATGTCTTAAGACACTACATACCTAATATTAAATACAGTAGCAAGCATGGGTTGATTTTTAAAATACCT
TGTCCTATTAATTTCTCAAAACACTACTCAGGCAGCATCTCTCCATGTTATACATCTAACCTAGGAACAAAATTAGAAAC
AGAAATACGTTACTCAATTAAACCAGGAACTGGATTATTAATTTTAGATCTCGTAGAAAATAATAAAATGTGTAATAAAA
AATTTTCCAACAATTACCATAACAGATATTGGCAATTATACTGGAAACATAATGGTATTATGAATAAAAAATGGCATTTT
AATTCGAATTATATCTCAACTATAGGTTCCAATAATTATATTGAAAATATCAATATACCAACATATACACACTCTAATAA
TAATTGTATGAACCAAAAAATACTGTGCAATTATACTGATAAAAATTGGAACGCTAATTTATCTTATTTTGGTATCACAA
ATATTAATATTACGACAATTAAAGACTATTATAATTACAACGAATCGTTCCAACTAGAATTAAATTCATATTATGATAAC
ATCAAGCATAAACCATTAAATTTAAAGTTATTTAGTCAAATAACCCAATTTATACCTGTAAATCATCATTATCCAAAAAC
AATCAGAATACACACGGAACCAACTTTAAATTTCTCAATAAATAATTATTATGGCCGTTTTAATACTGAAGCCAAATTAA
AAATAACACATTATCGACAAAAAAATATAAATCATTATAACAGAACACAATATCCAAACTATCATTTAGAAAATATAGTT
AATCGTATCGTCCCGCAATTAAAAATCAACGGAAAAATGATTTTAAAAAAAAACGCGCATATTTCAAAAAAATATAAACG
TTTTTTAGAACCAAAATTACAATATTTATATGTACCATATCGTTTTCAAGAAAACATTGGTATCTATGATACTGATATGA
TTCATATAGATCATAAAAATTTATTTCGTGACACTACATATAGTGGATTGGATCGTATTTTACCTGTTAATCAAATTACA
GGAGATATAACCATACGATGTTTTAAGAAAAAAAAAGAATTATTTTATGCATCTATAGGTCAAATTTTGAATTTAAGAAA
ACTTAATTCCGAATATATAAAAATATCTAAATGCACACAAAACACTCCTAATATTCTGTTATTTTCAGGAATAAGTCATT
GGAATATTAACGATTATTGGCATATGAACACCGAAACGCAATATGATATGCAACGTCATACATTATCATTTGGAACTATA
ATATTAGAATACACAGGTAAAAACAATCAAGTTTTACAAACACATTATAGATATACCAACGAACGATATCTTGAAAAAAT
ACTACCAGATATTAAAGCACCAATATACCACAACACAATTGCTCAATTAGGCGCACTCGCTTGTTATCCTTTAATTAATA
ATTGGAAAATAAGTTGCGCATATTACCATAACATAAAAACTAATCAACTCATTGATCAAGCAATAGGAATAGAGTATTAT
ACGCCATGTTGGGGGATTAATGTATCTTGTGAGCGAAAAATAATTGATTGGAACAAACAATTGAACCGTAATACATATGA
TAATAAAATACGATTAGATATCAAAATATATAGCTCTACATCAGATTTTAAAGCAAACCCATATAAAATGTTGGACATAG
GCATATTTCCTTACCAACGTACATTTTAA

Upstream 100 bases:

>100_bases
TAATATTCAGTAATAAATATACTATATAATATACTTATTTATCCAAGAATAAAGAGTTCATATTCTTTTTTGAATAACAT
GTATCAAAATAGTTTAACCA

Downstream 100 bases:

>100_bases
CCTTAAATATCACATGAAGTCAAATTCGTGCTTATAATATTGTTAATAATATGAAATTTTGGAAAATATTCATTCTAATA
TTTACGTTAAAAACAAATAT

Product: organic solvent tolerance protein precursor

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MIIKFFFKNTIINSIFIIFYTQFYFLAHASNCLQIPVANNLFSIDQPIYVSSDEIDIYYPNKIQFSGDVNMKQDNNIFMS
DKLIISLNEKNELLYNTLYACGHVSYNNNYIILTGSHAWINFYNKNIDIHEGTYHFSEPPIHGTANSIMQRGKNRYTIVK
QGKCTSCVSNKNYWNITGSEMLYDHHKHNIDVWNACLKIKKIPIFYSPYLSLSLHQNNVLRHYIPNIKYSSKHGLIFKIP
CPINFSKHYSGSISPCYTSNLGTKLETEIRYSIKPGTGLLILDLVENNKMCNKKFSNNYHNRYWQLYWKHNGIMNKKWHF
NSNYISTIGSNNYIENINIPTYTHSNNNCMNQKILCNYTDKNWNANLSYFGITNINITTIKDYYNYNESFQLELNSYYDN
IKHKPLNLKLFSQITQFIPVNHHYPKTIRIHTEPTLNFSINNYYGRFNTEAKLKITHYRQKNINHYNRTQYPNYHLENIV
NRIVPQLKINGKMILKKNAHISKKYKRFLEPKLQYLYVPYRFQENIGIYDTDMIHIDHKNLFRDTTYSGLDRILPVNQIT
GDITIRCFKKKKELFYASIGQILNLRKLNSEYIKISKCTQNTPNILLFSGISHWNINDYWHMNTETQYDMQRHTLSFGTI
ILEYTGKNNQVLQTHYRYTNERYLEKILPDIKAPIYHNTIAQLGALACYPLINNWKISCAYYHNIKTNQLIDQAIGIEYY
TPCWGINVSCERKIIDWNKQLNRNTYDNKIRLDIKIYSSTSDFKANPYKMLDIGIFPYQRTF

Sequences:

>Translated_782_residues
MIIKFFFKNTIINSIFIIFYTQFYFLAHASNCLQIPVANNLFSIDQPIYVSSDEIDIYYPNKIQFSGDVNMKQDNNIFMS
DKLIISLNEKNELLYNTLYACGHVSYNNNYIILTGSHAWINFYNKNIDIHEGTYHFSEPPIHGTANSIMQRGKNRYTIVK
QGKCTSCVSNKNYWNITGSEMLYDHHKHNIDVWNACLKIKKIPIFYSPYLSLSLHQNNVLRHYIPNIKYSSKHGLIFKIP
CPINFSKHYSGSISPCYTSNLGTKLETEIRYSIKPGTGLLILDLVENNKMCNKKFSNNYHNRYWQLYWKHNGIMNKKWHF
NSNYISTIGSNNYIENINIPTYTHSNNNCMNQKILCNYTDKNWNANLSYFGITNINITTIKDYYNYNESFQLELNSYYDN
IKHKPLNLKLFSQITQFIPVNHHYPKTIRIHTEPTLNFSINNYYGRFNTEAKLKITHYRQKNINHYNRTQYPNYHLENIV
NRIVPQLKINGKMILKKNAHISKKYKRFLEPKLQYLYVPYRFQENIGIYDTDMIHIDHKNLFRDTTYSGLDRILPVNQIT
GDITIRCFKKKKELFYASIGQILNLRKLNSEYIKISKCTQNTPNILLFSGISHWNINDYWHMNTETQYDMQRHTLSFGTI
ILEYTGKNNQVLQTHYRYTNERYLEKILPDIKAPIYHNTIAQLGALACYPLINNWKISCAYYHNIKTNQLIDQAIGIEYY
TPCWGINVSCERKIIDWNKQLNRNTYDNKIRLDIKIYSSTSDFKANPYKMLDIGIFPYQRTF
>Mature_782_residues
MIIKFFFKNTIINSIFIIFYTQFYFLAHASNCLQIPVANNLFSIDQPIYVSSDEIDIYYPNKIQFSGDVNMKQDNNIFMS
DKLIISLNEKNELLYNTLYACGHVSYNNNYIILTGSHAWINFYNKNIDIHEGTYHFSEPPIHGTANSIMQRGKNRYTIVK
QGKCTSCVSNKNYWNITGSEMLYDHHKHNIDVWNACLKIKKIPIFYSPYLSLSLHQNNVLRHYIPNIKYSSKHGLIFKIP
CPINFSKHYSGSISPCYTSNLGTKLETEIRYSIKPGTGLLILDLVENNKMCNKKFSNNYHNRYWQLYWKHNGIMNKKWHF
NSNYISTIGSNNYIENINIPTYTHSNNNCMNQKILCNYTDKNWNANLSYFGITNINITTIKDYYNYNESFQLELNSYYDN
IKHKPLNLKLFSQITQFIPVNHHYPKTIRIHTEPTLNFSINNYYGRFNTEAKLKITHYRQKNINHYNRTQYPNYHLENIV
NRIVPQLKINGKMILKKNAHISKKYKRFLEPKLQYLYVPYRFQENIGIYDTDMIHIDHKNLFRDTTYSGLDRILPVNQIT
GDITIRCFKKKKELFYASIGQILNLRKLNSEYIKISKCTQNTPNILLFSGISHWNINDYWHMNTETQYDMQRHTLSFGTI
ILEYTGKNNQVLQTHYRYTNERYLEKILPDIKAPIYHNTIAQLGALACYPLINNWKISCAYYHNIKTNQLIDQAIGIEYY
TPCWGINVSCERKIIDWNKQLNRNTYDNKIRLDIKIYSSTSDFKANPYKMLDIGIFPYQRTF

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=781, Percent_Identity=31.6261203585147, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 92650; Mature: 92650

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKFFFKNTIINSIFIIFYTQFYFLAHASNCLQIPVANNLFSIDQPIYVSSDEIDIYYP
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCEEECCCEEEEEEC
NKIQFSGDVNMKQDNNIFMSDKLIISLNEKNELLYNTLYACGHVSYNNNYIILTGSHAWI
CEEEECCCCCCCCCCCEEEECEEEEEECCCCCHHHHHHHHHEEEEECCCEEEEECCCEEE
NFYNKNIDIHEGTYHFSEPPIHGTANSIMQRGKNRYTIVKQGKCTSCVSNKNYWNITGSE
EEEECEEEEECCEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHCCCCEEEECCCH
MLYDHHKHNIDVWNACLKIKKIPIFYSPYLSLSLHQNNVLRHYIPNIKYSSKHGLIFKIP
HEECCCCCCCHHHHHEEEEEECEEEECCCEEEEECCCCCHHHHCCCCEECCCCCEEEEEC
CPINFSKHYSGSISPCYTSNLGTKLETEIRYSIKPGTGLLILDLVENNKMCNKKFSNNYH
CCCCCCCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCEEEEEEECCCCHHCHHHCCCCC
NRYWQLYWKHNGIMNKKWHFNSNYISTIGSNNYIENINIPTYTHSNNNCMNQKILCNYTD
CEEEEEEEEECCCCCCEEEECCCEEEECCCCCEEEECCCCEEECCCCCCCCCEEEEEECC
KNWNANLSYFGITNINITTIKDYYNYNESFQLELNSYYDNIKHKPLNLKLFSQITQFIPV
CCCCCCEEEEEEEEEEEEEEEHHCCCCCEEEEEEEHHHCCCCCCCCCCHHHHHHHHHCCC
NHHYPKTIRIHTEPTLNFSINNYYGRFNTEAKLKITHYRQKNINHYNRTQYPNYHLENIV
CCCCCCEEEEECCCEEEEEEECEEEEECCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHH
NRIVPQLKINGKMILKKNAHISKKYKRFLEPKLQYLYVPYRFQENIGIYDTDMIHIDHKN
HHHCCEEEECCEEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEEEECCHH
LFRDTTYSGLDRILPVNQITGDITIRCFKKKKELFYASIGQILNLRKLNSEYIKISKCTQ
HHCCCCHHHHHHCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
NTPNILLFSGISHWNINDYWHMNTETQYDMQRHTLSFGTIILEYTGKNNQVLQTHYRYTN
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHEEEEEEEEEEEECCCCCEEEEEHHCCH
ERYLEKILPDIKAPIYHNTIAQLGALACYPLINNWKISCAYYHNIKTNQLIDQAIGIEYY
HHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCCEEEEEEEEECCCHHHHHHHHCCCEEE
TPCWGINVSCERKIIDWNKQLNRNTYDNKIRLDIKIYSSTSDFKANPYKMLDIGIFPYQR
CCEECCCCCCCCEEECCCHHCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEEEECCCCC
TF
CC
>Mature Secondary Structure
MIIKFFFKNTIINSIFIIFYTQFYFLAHASNCLQIPVANNLFSIDQPIYVSSDEIDIYYP
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCEEECCCEEEEEEC
NKIQFSGDVNMKQDNNIFMSDKLIISLNEKNELLYNTLYACGHVSYNNNYIILTGSHAWI
CEEEECCCCCCCCCCCEEEECEEEEEECCCCCHHHHHHHHHEEEEECCCEEEEECCCEEE
NFYNKNIDIHEGTYHFSEPPIHGTANSIMQRGKNRYTIVKQGKCTSCVSNKNYWNITGSE
EEEECEEEEECCEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHCCCCEEEECCCH
MLYDHHKHNIDVWNACLKIKKIPIFYSPYLSLSLHQNNVLRHYIPNIKYSSKHGLIFKIP
HEECCCCCCCHHHHHEEEEEECEEEECCCEEEEECCCCCHHHHCCCCEECCCCCEEEEEC
CPINFSKHYSGSISPCYTSNLGTKLETEIRYSIKPGTGLLILDLVENNKMCNKKFSNNYH
CCCCCCCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCEEEEEEECCCCHHCHHHCCCCC
NRYWQLYWKHNGIMNKKWHFNSNYISTIGSNNYIENINIPTYTHSNNNCMNQKILCNYTD
CEEEEEEEEECCCCCCEEEECCCEEEECCCCCEEEECCCCEEECCCCCCCCCEEEEEECC
KNWNANLSYFGITNINITTIKDYYNYNESFQLELNSYYDNIKHKPLNLKLFSQITQFIPV
CCCCCCEEEEEEEEEEEEEEEHHCCCCCEEEEEEEHHHCCCCCCCCCCHHHHHHHHHCCC
NHHYPKTIRIHTEPTLNFSINNYYGRFNTEAKLKITHYRQKNINHYNRTQYPNYHLENIV
CCCCCCEEEEECCCEEEEEEECEEEEECCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHH
NRIVPQLKINGKMILKKNAHISKKYKRFLEPKLQYLYVPYRFQENIGIYDTDMIHIDHKN
HHHCCEEEECCEEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEEEECCHH
LFRDTTYSGLDRILPVNQITGDITIRCFKKKKELFYASIGQILNLRKLNSEYIKISKCTQ
HHCCCCHHHHHHCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
NTPNILLFSGISHWNINDYWHMNTETQYDMQRHTLSFGTIILEYTGKNNQVLQTHYRYTN
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHEEEEEEEEEEEECCCCCEEEEEHHCCH
ERYLEKILPDIKAPIYHNTIAQLGALACYPLINNWKISCAYYHNIKTNQLIDQAIGIEYY
HHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCCEEEEEEEEECCCHHHHHHHHCCCEEE
TPCWGINVSCERKIIDWNKQLNRNTYDNKIRLDIKIYSSTSDFKANPYKMLDIGIFPYQR
CCEECCCCCCCCEEECCCHHCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEEEECCCCC
TF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA