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The map label for this gene is folA [H]

Identifier: 71891911

GI number: 71891911

Start: 156320

End: 156814

Strand: Direct

Name: folA [H]

Synonym: BPEN_128

Alternate gene names: 71891911

Gene position: 156320-156814 (Clockwise)

Preceding gene: 71891910

Following gene: 71891921

Centisome position: 19.75

GC content: 27.47

Gene sequence:

>495_bases
ATGATTATTAGCTTAATTGCTGCGTTGACTACCAATCATATAATTGGAAAAAAAAATGTTATTCCTTGGTATTTACCTAT
AGATATTAAGTGGTTTAAGTATCATACTTTATATAAGCCAATTATTATGGGACGCAAAACATTCGAGTCTATAGGTAAGA
AACCATTATTAAATAGATTGAATATTGTGTTAAGTCGTAATTTATTAAATAATTATAATGGTGTTTTTATAGCGGAAAAT
ATAGATGAAGCTTTATCTTTAATACAGGACGCATATGAAGTTATGGTAATTGGAGGCAGTGAAATATACAATGTTTTTTT
ACCTCGTGCGCAACGCTTATATTTAACATATATTCATAACATGGTTGAAATTGATGGTGACATTTTGTTTCCTGATTATG
ATATAAGAGAATGGAAATCTATTTTCAATAGTTTCTATAAAGTTAGAGAGGATTGTTTTTGTTATTTGCATTTTTCTATT
TTAGAACGCTACTAA

Upstream 100 bases:

>100_bases
TAGTATATATTGCAGAATTACACTTGGTATGTTTCTGTCATTATAGATTTTATTAAAAAGTATTTTATAATTTTACATAC
ACGTTGAACGTTATGCCATA

Downstream 100 bases:

>100_bases
TAAAAATTAGTATGATTAATAATAAACATTATGTTAATGAAACCAATCTTGCATATTAATAAATTATTTTTAAAAGAGTG
ATAATTACAATATGGTACTA

Product: dihydrofolate reductase type I

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MIISLIAALTTNHIIGKKNVIPWYLPIDIKWFKYHTLYKPIIMGRKTFESIGKKPLLNRLNIVLSRNLLNNYNGVFIAEN
IDEALSLIQDAYEVMVIGGSEIYNVFLPRAQRLYLTYIHNMVEIDGDILFPDYDIREWKSIFNSFYKVREDCFCYLHFSI
LERY

Sequences:

>Translated_164_residues
MIISLIAALTTNHIIGKKNVIPWYLPIDIKWFKYHTLYKPIIMGRKTFESIGKKPLLNRLNIVLSRNLLNNYNGVFIAEN
IDEALSLIQDAYEVMVIGGSEIYNVFLPRAQRLYLTYIHNMVEIDGDILFPDYDIREWKSIFNSFYKVREDCFCYLHFSI
LERY
>Mature_164_residues
MIISLIAALTTNHIIGKKNVIPWYLPIDIKWFKYHTLYKPIIMGRKTFESIGKKPLLNRLNIVLSRNLLNNYNGVFIAEN
IDEALSLIQDAYEVMVIGGSEIYNVFLPRAQRLYLTYIHNMVEIDGDILFPDYDIREWKSIFNSFYKVREDCFCYLHFSI
LERY

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=156, Percent_Identity=31.4102564102564, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=46.9135802469136, Blast_Score=151, Evalue=2e-38,
Organism=Drosophila melanogaster, GI116008026, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24647458, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19422; Mature: 19422

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISLIAALTTNHIIGKKNVIPWYLPIDIKWFKYHTLYKPIIMGRKTFESIGKKPLLNRL
CHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHCHHHHHHCCCCHHHHHH
NIVLSRNLLNNYNGVFIAENIDEALSLIQDAYEVMVIGGSEIYNVFLPRAQRLYLTYIHN
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHEEEECCHHHHHHHHCCHHHHHHHHHHH
MVEIDGDILFPDYDIREWKSIFNSFYKVREDCFCYLHFSILERY
HHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIISLIAALTTNHIIGKKNVIPWYLPIDIKWFKYHTLYKPIIMGRKTFESIGKKPLLNRL
CHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHCHHHHHHCCCCHHHHHH
NIVLSRNLLNNYNGVFIAENIDEALSLIQDAYEVMVIGGSEIYNVFLPRAQRLYLTYIHN
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHEEEECCHHHHHHHHCCHHHHHHHHHHH
MVEIDGDILFPDYDIREWKSIFNSFYKVREDCFCYLHFSILERY
HHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]