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The map label for this gene is purA

Identifier: 71891872

GI number: 71891872

Start: 101746

End: 103044

Strand: Direct

Name: purA

Synonym: BPEN_086

Alternate gene names: 71891872

Gene position: 101746-103044 (Clockwise)

Preceding gene: 71891871

Following gene: 71891873

Centisome position: 12.85

GC content: 35.41

Gene sequence:

>1299_bases
ATGGCTAGAAGTATTGTTGTGTTAGGAGCTCAGTGGGGTGATGAAGGTAAAGGAAAAATAGTTGATTGGTTAACTTCCCG
AGCGCAATATGTAGTCCGTTATCAAGGGGGTCACAATGCTGGTCATACTATTGTAGTAGACCAGCAGAAAATAACTCTAC
ATTTAATTCCTTCTGGAATTTTGCATAATCATGTTACTGCTGTTATTGCTAACGGTGTTGTATTATCGCCATTTTCTTTA
ATAAAAGAAATGAAAATGTTACTAAAACTAGGTGTGTCAACGTGTAAACGCATATTTATTTCTGCATCTTGTCCTTTGTT
ACTACCTTATCATGTAGCAATGGATTTGGCTAGAGAAAATCATCACATCTCTAAAGCTATTGGTACCACTGGGTGTGGTA
TTGGTCCATCATATGAGGATAAAGTTGCGCGCCGTGCTTTACGAGTCAGCGATTTGTATAATCTAAAATATTTTAAAAAT
AAGTTGAAAGATGTGGTCGATTATTATAATTTTCAATTAGTTTATTATTATAAAACGAAGCCTATCAATTATCAGATTGT
TTTAGAGGAAGTTATGTCGATATCTGACATACTGATAGATATGGTTGTCGATGTACCTGAGTTATTAGATGATGCCGCAA
AACGGGGTGATTCAGTAATATTTGAGGGAGCACAAGGTAGTTTATTAGATATTGATCATGGAACATATCCTTATGTTACT
TCCAGTCATACTATTGCTGGGAGTGTAAGTGTTGGTGCAGGAGTTGGGCTTAATTATATTGATTATGTTTTAGGTATTGT
AAAGGCGTATTCTACTCGTGTGGGATTTGGGCCCTTCCCTACAGAATTGTCAAATGATACAGGTAATTGGTTATGTATGA
ATGGAAATGAATTTGGTTCTACTACTGGTCGCCGTCGTCGTACTGGTTGGTTTGATGCGGTATCAGTACGATATTCTGTA
AAAATTAATTCTTTTTTTTCGTGTTGTTTAACAAAGATTGATGTTTTAGATGGCTTAAAAGAGCTAAAAATTTGTATTGC
ATATCGCAAGAAAAATGGAAAAGTAATACACAATTTTCCATGTTCTTTGGAAGAACTAGAAAATTGTGTACCTATATACG
AAATATTACCTGGGTGGATGATAAGTACTGTAGGTATCACGGAATTCCATCAACTTCCTAAGGAGTCACAGCTGTATGTA
AAACGTATAGAAGAACTTATTGGGGTTCCTATACATATTGTTTCTACGGGATCAGACCGCTCTGCAATAATAATATTACG
TAATCCATTTGATTCTTGA

Upstream 100 bases:

>100_bases
AGAGTATAGAGCAAATATTATATATGGTGTATTTCATAGAAACTATATATTTGATTTTATCACAAATAGTTAATTTGAAT
TAAATAGGCGACTTTAAGAA

Downstream 100 bases:

>100_bases
TTAGTAGTATGAAGAAATTAATACCTTATTTTGAATAGAAGATTATTTGTGTGGTTAATAAATATTAAACTATATTGCTA
ATTATATTAGACATACAGTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MARSIVVLGAQWGDEGKGKIVDWLTSRAQYVVRYQGGHNAGHTIVVDQQKITLHLIPSGILHNHVTAVIANGVVLSPFSL
IKEMKMLLKLGVSTCKRIFISASCPLLLPYHVAMDLARENHHISKAIGTTGCGIGPSYEDKVARRALRVSDLYNLKYFKN
KLKDVVDYYNFQLVYYYKTKPINYQIVLEEVMSISDILIDMVVDVPELLDDAAKRGDSVIFEGAQGSLLDIDHGTYPYVT
SSHTIAGSVSVGAGVGLNYIDYVLGIVKAYSTRVGFGPFPTELSNDTGNWLCMNGNEFGSTTGRRRRTGWFDAVSVRYSV
KINSFFSCCLTKIDVLDGLKELKICIAYRKKNGKVIHNFPCSLEELENCVPIYEILPGWMISTVGITEFHQLPKESQLYV
KRIEELIGVPIHIVSTGSDRSAIIILRNPFDS

Sequences:

>Translated_432_residues
MARSIVVLGAQWGDEGKGKIVDWLTSRAQYVVRYQGGHNAGHTIVVDQQKITLHLIPSGILHNHVTAVIANGVVLSPFSL
IKEMKMLLKLGVSTCKRIFISASCPLLLPYHVAMDLARENHHISKAIGTTGCGIGPSYEDKVARRALRVSDLYNLKYFKN
KLKDVVDYYNFQLVYYYKTKPINYQIVLEEVMSISDILIDMVVDVPELLDDAAKRGDSVIFEGAQGSLLDIDHGTYPYVT
SSHTIAGSVSVGAGVGLNYIDYVLGIVKAYSTRVGFGPFPTELSNDTGNWLCMNGNEFGSTTGRRRRTGWFDAVSVRYSV
KINSFFSCCLTKIDVLDGLKELKICIAYRKKNGKVIHNFPCSLEELENCVPIYEILPGWMISTVGITEFHQLPKESQLYV
KRIEELIGVPIHIVSTGSDRSAIIILRNPFDS
>Mature_431_residues
ARSIVVLGAQWGDEGKGKIVDWLTSRAQYVVRYQGGHNAGHTIVVDQQKITLHLIPSGILHNHVTAVIANGVVLSPFSLI
KEMKMLLKLGVSTCKRIFISASCPLLLPYHVAMDLARENHHISKAIGTTGCGIGPSYEDKVARRALRVSDLYNLKYFKNK
LKDVVDYYNFQLVYYYKTKPINYQIVLEEVMSISDILIDMVVDVPELLDDAAKRGDSVIFEGAQGSLLDIDHGTYPYVTS
SHTIAGSVSVGAGVGLNYIDYVLGIVKAYSTRVGFGPFPTELSNDTGNWLCMNGNEFGSTTGRRRRTGWFDAVSVRYSVK
INSFFSCCLTKIDVLDGLKELKICIAYRKKNGKVIHNFPCSLEELENCVPIYEILPGWMISTVGITEFHQLPKESQLYVK
RIEELIGVPIHIVSTGSDRSAIIILRNPFDS

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=434, Percent_Identity=40.7834101382488, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI34577063, Length=435, Percent_Identity=40.4597701149425, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI40316944, Length=401, Percent_Identity=38.9027431421446, Blast_Score=265, Evalue=6e-71,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=61.5740740740741, Blast_Score=575, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=39.6270396270396, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=39.6270396270396, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=40.6976744186046, Blast_Score=305, Evalue=9e-84,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=40.855106888361, Blast_Score=316, Evalue=2e-86,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_BLOPB (Q494F3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277601.1
- ProteinModelPortal:   Q494F3
- SMR:   Q494F3
- STRING:   Q494F3
- GeneID:   3563190
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_086
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVQPMK
- ProtClustDB:   PRK01117
- BioCyc:   CBLO291272:BPEN_086-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48034; Mature: 47903

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSIVVLGAQWGDEGKGKIVDWLTSRAQYVVRYQGGHNAGHTIVVDQQKITLHLIPSGI
CCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEECCEEEEEECCCHH
LHNHVTAVIANGVVLSPFSLIKEMKMLLKLGVSTCKRIFISASCPLLLPYHVAMDLAREN
HHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHCC
HHISKAIGTTGCGIGPSYEDKVARRALRVSDLYNLKYFKNKLKDVVDYYNFQLVYYYKTK
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECC
PINYQIVLEEVMSISDILIDMVVDVPELLDDAAKRGDSVIFEGAQGSLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSHTIAGSVSVGAGVGLNYIDYVLGIVKAYSTRVGFGPFPTELSNDTGNWLCMNGNEFGS
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCC
TTGRRRRTGWFDAVSVRYSVKINSFFSCCLTKIDVLDGLKELKICIAYRKKNGKVIHNFP
CCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECC
CSLEELENCVPIYEILPGWMISTVGITEFHQLPKESQLYVKRIEELIGVPIHIVSTGSDR
CCHHHHHCCCCHHHHCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCC
SAIIILRNPFDS
EEEEEEECCCCC
>Mature Secondary Structure 
ARSIVVLGAQWGDEGKGKIVDWLTSRAQYVVRYQGGHNAGHTIVVDQQKITLHLIPSGI
CCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEECCEEEEEECCCHH
LHNHVTAVIANGVVLSPFSLIKEMKMLLKLGVSTCKRIFISASCPLLLPYHVAMDLAREN
HHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHCC
HHISKAIGTTGCGIGPSYEDKVARRALRVSDLYNLKYFKNKLKDVVDYYNFQLVYYYKTK
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECC
PINYQIVLEEVMSISDILIDMVVDVPELLDDAAKRGDSVIFEGAQGSLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSHTIAGSVSVGAGVGLNYIDYVLGIVKAYSTRVGFGPFPTELSNDTGNWLCMNGNEFGS
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCC
TTGRRRRTGWFDAVSVRYSVKINSFFSCCLTKIDVLDGLKELKICIAYRKKNGKVIHNFP
CCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECC
CSLEELENCVPIYEILPGWMISTVGITEFHQLPKESQLYVKRIEELIGVPIHIVSTGSDR
CCHHHHHCCCCHHHHCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCC
SAIIILRNPFDS
EEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA