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The map label for this gene is rlmB [H]

Identifier: 71891873

GI number: 71891873

Start: 103280

End: 104023

Strand: Direct

Name: rlmB [H]

Synonym: BPEN_087

Alternate gene names: 71891873

Gene position: 103280-104023 (Clockwise)

Preceding gene: 71891872

Following gene: 71891874

Centisome position: 13.05

GC content: 34.27

Gene sequence:

>744_bases
ATGAGTGAATTAGTATACGGCATACATGCAGTAAAATCGGTTTTAGATAATTATTCGCATCGAATTGTGTCTGTATGTAT
CATTCAGAAATCTAAAGATTTGCGTTTGAAATTGTTAATTGATCAAATAAAACAACGTCGAATTAGCATTCGAGAATGTA
GTCGTCGACAGTTAGATATTCAAACGCAAGGTGCGTTGCATCAAGGTATTATTGTTAAAATAGTTCCAAATGATTGTATG
CAAGAAAATGATTTACCGAATTTTTTAATGCAATGTAAAACTACACCATTATTAGTGATATTAGATGGTGTGACTGATCC
ACATAATTTGGGTGCATGTTTGAGATCTGCAGATGCAGCTGGGGCACACATAGTCATTGCACCACGTAATCGTTCAGCAT
GTTTAAATGGGACGGTTAGAAAAGTGGCTAGTGGATCTGTAGATAATATACCTTTTATGCAAGTAACTAATTTAATTAGA
ACGTTACAATTACTTCAAAAATATAATATCTGGATTATTGGCACTGTAATGCAATCTCAACGTCTAATTTTTAACAGCAA
ATTAACTAGGCCATTAGCTTTTGTAATGGGTTCTGAGGGAAATGGTATTCGTTATCTGACTCAGAAAAAATGCGATGAAC
TAGTTACGATCCCAATGTTAGGAGTTGCGACATCGCTTAATGTTTCAGTAGCTACGGGAATATGTTTGTTTGAAGCATTA
AGACAGCGTTGGAAATATAAATAA

Upstream 100 bases:

>100_bases
TATATTTCAACCGATGCCTTTCATAATAATGGTATATAAAAAATACTTTAAAAATTTATTGCAATACTGTGTGTTGAAGT
AATTTATTTTAATAAATGAA

Downstream 100 bases:

>100_bases
TGATGATTGAAATGATATATGATATGGTCATCTTCGTAGAAGATGAAGTTTACGAATTTATATAAAAAATCATTTAAGGA
TAAACACGTAAAAATATAAA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSELVYGIHAVKSVLDNYSHRIVSVCIIQKSKDLRLKLLIDQIKQRRISIRECSRRQLDIQTQGALHQGIIVKIVPNDCM
QENDLPNFLMQCKTTPLLVILDGVTDPHNLGACLRSADAAGAHIVIAPRNRSACLNGTVRKVASGSVDNIPFMQVTNLIR
TLQLLQKYNIWIIGTVMQSQRLIFNSKLTRPLAFVMGSEGNGIRYLTQKKCDELVTIPMLGVATSLNVSVATGICLFEAL
RQRWKYK

Sequences:

>Translated_247_residues
MSELVYGIHAVKSVLDNYSHRIVSVCIIQKSKDLRLKLLIDQIKQRRISIRECSRRQLDIQTQGALHQGIIVKIVPNDCM
QENDLPNFLMQCKTTPLLVILDGVTDPHNLGACLRSADAAGAHIVIAPRNRSACLNGTVRKVASGSVDNIPFMQVTNLIR
TLQLLQKYNIWIIGTVMQSQRLIFNSKLTRPLAFVMGSEGNGIRYLTQKKCDELVTIPMLGVATSLNVSVATGICLFEAL
RQRWKYK
>Mature_246_residues
SELVYGIHAVKSVLDNYSHRIVSVCIIQKSKDLRLKLLIDQIKQRRISIRECSRRQLDIQTQGALHQGIIVKIVPNDCMQ
ENDLPNFLMQCKTTPLLVILDGVTDPHNLGACLRSADAAGAHIVIAPRNRSACLNGTVRKVASGSVDNIPFMQVTNLIRT
LQLLQKYNIWIIGTVMQSQRLIFNSKLTRPLAFVMGSEGNGIRYLTQKKCDELVTIPMLGVATSLNVSVATGICLFEALR
QRWKYK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=265, Percent_Identity=28.6792452830189, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=53.4979423868313, Blast_Score=274, Evalue=4e-75,
Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=33.9869281045752, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788935, Length=241, Percent_Identity=23.2365145228216, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=285, Percent_Identity=22.8070175438596, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27654; Mature: 27523

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELVYGIHAVKSVLDNYSHRIVSVCIIQKSKDLRLKLLIDQIKQRRISIRECSRRQLDI
CCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QTQGALHQGIIVKIVPNDCMQENDLPNFLMQCKTTPLLVILDGVTDPHNLGACLRSADAA
CCCCCCCCCEEEEECCCHHCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCC
GAHIVIAPRNRSACLNGTVRKVASGSVDNIPFMQVTNLIRTLQLLQKYNIWIIGTVMQSQ
CCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEHHCC
RLIFNSKLTRPLAFVMGSEGNGIRYLTQKKCDELVTIPMLGVATSLNVSVATGICLFEAL
HHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHHHH
RQRWKYK
HHHHCCC
>Mature Secondary Structure 
SELVYGIHAVKSVLDNYSHRIVSVCIIQKSKDLRLKLLIDQIKQRRISIRECSRRQLDI
CHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QTQGALHQGIIVKIVPNDCMQENDLPNFLMQCKTTPLLVILDGVTDPHNLGACLRSADAA
CCCCCCCCCEEEEECCCHHCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCC
GAHIVIAPRNRSACLNGTVRKVASGSVDNIPFMQVTNLIRTLQLLQKYNIWIIGTVMQSQ
CCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEHHCC
RLIFNSKLTRPLAFVMGSEGNGIRYLTQKKCDELVTIPMLGVATSLNVSVATGICLFEAL
HHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHHHH
RQRWKYK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]