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The map label for this gene is dnaN [H]

Identifier: 71891808

GI number: 71891808

Start: 15161

End: 16264

Strand: Direct

Name: dnaN [H]

Synonym: BPEN_015

Alternate gene names: 71891808

Gene position: 15161-16264 (Clockwise)

Preceding gene: 71891803

Following gene: 71891809

Centisome position: 1.92

GC content: 30.62

Gene sequence:

>1104_bases
ATGAATTTTGTAATAGATAGGAAATTATTATATCAATCTTTGCAAAAAGTAGTGGGCATTCTTAGCGGACGCCCGAGAAT
GCCCATATTAACTCATGTGTTATTAGAAGTGAATGATAATTGCTTATGTATTACTGCTACGGACTTAGAAATAGAGATAA
CAGTTCGCGTTATATTACAAGATAAATATCCCATTGGGTCTGTGATAGTACCAGGGCGTAAGTTTTTTGAAATTTGTCGT
AGTTTATCTGAATCATCAAAAATCTCCATAGTATCAAAAGACAAAAAAATGATTATTTGTTCTGGATGTAGTAAATTTTA
TTTATCTACGTTTTCTTCGTTAGATTTTCCTAAATTAGAAAAATGGGGCAATAATCTAAAATTAGTTATATCCCAGGCTA
TTTTAAAAAAAATGATTGAATTAACTCAATTTTCTATGGGCCATCAAGATGTACGTTATTGCCTAAATGGGATATTATTT
GAAACTAAAGAACATATTTTGCGTATTGTTGCGACAGATGGACATCGATTAGCAAGTTGTGAAATAACTATGGATTTATT
ATTACCCAATCAATCATCGATTATACCTCGTAAAGGAGTTATCGAAATCTTACGTTTGTTAAACGTTAAAGAAAAATTAG
TTAATATTCAAACAAATAGTAATAATCTCCGAATGGAGACAGAGAATTATACTTTTACTTCTAAATTGATTGATGCTGTG
TTTCCAAATTGTCATGATGTTTTTATAAAGCGTTCAAAAAATGTTTTGGAAGTAGAATGCGGTATTTTGAAGCAGGCTTT
AAAACGTGCAGCTATTCTTGCAAATGAAAAACTTCGTATCGTTCGATTTACATTAATTACTAATCAATTAAAAATAACCG
CGCATAATTTTGAACATGAAGCATCGGAAGAAATTTTAGACGTTTTATATTCAGGAAAGAGTATTAAAATATCTTTTAAT
GTAGATTATTTGATCGATGTTCTTCAGGTAATGGATACTCAGTTTTTACGGTTTTATTTGACAGATGAAATATCCAGCGT
GCAAATTGAGAGTACCGAGAAATGTCATGGAGCAACATATATCGTCATGCCAATTCGGATATAA

Upstream 100 bases:

>100_bases
TTTTACATATTATACTATCGCGATATGTGTTTGAGATTTTTAAAGTGATATCACATTGTTATGTGATTATTTAAAATGTT
GTCTTTTACTGAGTTTGGTT

Downstream 100 bases:

>100_bases
TAATGATTTACAGATTGTAACCTAACATGTATTTTAATGTTATCAAATGTGATTGAATTGATAGCACGCAGTAACAATAA
TACTAGAACATATAAATTAA

Product: DNA polymerase III, beta-subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNFVIDRKLLYQSLQKVVGILSGRPRMPILTHVLLEVNDNCLCITATDLEIEITVRVILQDKYPIGSVIVPGRKFFEICR
SLSESSKISIVSKDKKMIICSGCSKFYLSTFSSLDFPKLEKWGNNLKLVISQAILKKMIELTQFSMGHQDVRYCLNGILF
ETKEHILRIVATDGHRLASCEITMDLLLPNQSSIIPRKGVIEILRLLNVKEKLVNIQTNSNNLRMETENYTFTSKLIDAV
FPNCHDVFIKRSKNVLEVECGILKQALKRAAILANEKLRIVRFTLITNQLKITAHNFEHEASEEILDVLYSGKSIKISFN
VDYLIDVLQVMDTQFLRFYLTDEISSVQIESTEKCHGATYIVMPIRI

Sequences:

>Translated_367_residues
MNFVIDRKLLYQSLQKVVGILSGRPRMPILTHVLLEVNDNCLCITATDLEIEITVRVILQDKYPIGSVIVPGRKFFEICR
SLSESSKISIVSKDKKMIICSGCSKFYLSTFSSLDFPKLEKWGNNLKLVISQAILKKMIELTQFSMGHQDVRYCLNGILF
ETKEHILRIVATDGHRLASCEITMDLLLPNQSSIIPRKGVIEILRLLNVKEKLVNIQTNSNNLRMETENYTFTSKLIDAV
FPNCHDVFIKRSKNVLEVECGILKQALKRAAILANEKLRIVRFTLITNQLKITAHNFEHEASEEILDVLYSGKSIKISFN
VDYLIDVLQVMDTQFLRFYLTDEISSVQIESTEKCHGATYIVMPIRI
>Mature_367_residues
MNFVIDRKLLYQSLQKVVGILSGRPRMPILTHVLLEVNDNCLCITATDLEIEITVRVILQDKYPIGSVIVPGRKFFEICR
SLSESSKISIVSKDKKMIICSGCSKFYLSTFSSLDFPKLEKWGNNLKLVISQAILKKMIELTQFSMGHQDVRYCLNGILF
ETKEHILRIVATDGHRLASCEITMDLLLPNQSSIIPRKGVIEILRLLNVKEKLVNIQTNSNNLRMETENYTFTSKLIDAV
FPNCHDVFIKRSKNVLEVECGILKQALKRAAILANEKLRIVRFTLITNQLKITAHNFEHEASEEILDVLYSGKSIKISFN
VDYLIDVLQVMDTQFLRFYLTDEISSVQIESTEKCHGATYIVMPIRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=48.7738419618529, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41936; Mature: 41936

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVIDRKLLYQSLQKVVGILSGRPRMPILTHVLLEVNDNCLCITATDLEIEITVRVILQ
CCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCEEEEEECCEEEEEEEEEEEE
DKYPIGSVIVPGRKFFEICRSLSESSKISIVSKDKKMIICSGCSKFYLSTFSSLDFPKLE
CCCCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCCHHH
KWGNNLKLVISQAILKKMIELTQFSMGHQDVRYCLNGILFETKEHILRIVATDGHRLASC
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEE
EITMDLLLPNQSSIIPRKGVIEILRLLNVKEKLVNIQTNSNNLRMETENYTFTSKLIDAV
EEEEEEECCCCCCCCCCHHHHHHHHHHCHHHHHEEEEECCCCEEEEECCCCHHHHHHHHH
FPNCHDVFIKRSKNVLEVECGILKQALKRAAILANEKLRIVRFTLITNQLKITAHNFEHE
CCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEEEEEECEEEEEEECCCCH
ASEEILDVLYSGKSIKISFNVDYLIDVLQVMDTQFLRFYLTDEISSVQIESTEKCHGATY
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEE
IVMPIRI
EEEEEEC
>Mature Secondary Structure
MNFVIDRKLLYQSLQKVVGILSGRPRMPILTHVLLEVNDNCLCITATDLEIEITVRVILQ
CCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCEEEEEECCEEEEEEEEEEEE
DKYPIGSVIVPGRKFFEICRSLSESSKISIVSKDKKMIICSGCSKFYLSTFSSLDFPKLE
CCCCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCCHHH
KWGNNLKLVISQAILKKMIELTQFSMGHQDVRYCLNGILFETKEHILRIVATDGHRLASC
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEE
EITMDLLLPNQSSIIPRKGVIEILRLLNVKEKLVNIQTNSNNLRMETENYTFTSKLIDAV
EEEEEEECCCCCCCCCCHHHHHHHHHHCHHHHHEEEEECCCCEEEEECCCCHHHHHHHHH
FPNCHDVFIKRSKNVLEVECGILKQALKRAAILANEKLRIVRFTLITNQLKITAHNFEHE
CCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEEEEEECEEEEEEECCCCH
ASEEILDVLYSGKSIKISFNVDYLIDVLQVMDTQFLRFYLTDEISSVQIESTEKCHGATY
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEE
IVMPIRI
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]