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The map label for this gene is tgt [H]

Identifier: 68537115

GI number: 68537115

Start: 2358685

End: 2360133

Strand: Direct

Name: tgt [H]

Synonym: jk2020

Alternate gene names: 68537115

Gene position: 2358685-2360133 (Clockwise)

Preceding gene: 68537114

Following gene: 68537120

Centisome position: 95.78

GC content: 65.77

Gene sequence:

>1449_bases
ATGGCCGACAATCAGGAAAACACCAGCGCGGACGCGGGCGCGGGCGCCGCAGACCTGTCCTTCGAGGTGCGCACCCGGCT
GCGCGATAGCGAAAGTGCAAGCGCGGGAGCAGGCGCGGAAGGAAGCGCAGGCGCAGCTGCAAGCGCGGGAGCAGGCGCGG
AAGGAAGCGCAGGCGGAAGCGCGGGAGCAGGCGCAGGCGCAGGCGGAAGCGCGCGCCTTAACGGAAGCGCGGGCGCGCTG
GGGCGTACGGGCGTCATAAAGACTCCGCATGGGAACATCCACACCCCAGCTTTCATCCCGGTGGCGACGAAAGCAACGGT
CAAGACCCTCACGCCCGAGCAGGTGCGGTCGACGGGCGCGCAGGCGATGCTTTCCAACGCGTACCACCTGTACCTTCAGC
CGGGCCCGGACATTGTGGACGAGGGCGGCGGGCTCGCACAGTTCGAGAACTGGCACGGGCCGACGTACACGGACTCCGGC
GGCTTCCAAGTCATGAGCCTCGGCGTGGGCTACAAGAAAGTCCTGGCCATGGACACGAAAGGCCGCAGCGAGGCGGACAT
CATCCAGCAGCAGAAAAAGCGCATGGCCGTAGTGGACGAGGACGGCGTGGATTTCCGCAGCGTCATCGACGGCAGCAAGC
ACCGCTTCACGCCGGAGGTGTCGATGCAGATCCAGCACCAGCTGGGCGCGGACATTATGTTCGCGTTCGACGAGCTCACC
ACGCTGGTGAACACCCGCTCCTACCAGGAGCAATCCGTCGCACGTACGCACCGCTGGGCTCGCCGTTGCCTGGCGGAGCA
CCAGCGGCTGACGGACTTGCGCACACACCGCCCGCTGCAGTCTCTGTGGGGCGTGGTGCAGGGCGCGCAGTACGAGGATC
TGCGCAGGCAGGCGGCCCGTGGGCTGGTGCAGCTGTCCGAGGAGGCGGAGGCGCAGGGCAACCGTGGTTTCGGCGGCTTC
GGCATCGGCGGCGCGCTGGAGAAGGAGAACCTCGGCACGATCGTCCGCTGGGTTGCCGAGGAGTTGCCGGAGGATAAGCC
CCGCCACCTGCTCGGCATTTCGGAGCCGGATGACCTGTTCACGGCGATTGAGGCCGGCGCGGACACGTTCGATTGCGTCG
CGCCGACTCGCCTGGGCCGCCGCGGTGGCGTGTACACGCTGGACGGTCGGCTGAATCTTATGGGTGCGCGGTTTAAGAGG
GATTTTTCTCCTATTGACGCCGCAGTAGGCGGTTATGTCTCGGAGAATTATTCGCGCGCTTATATTCACCACTTGTTGAA
GGCGAAGGAGTACCTGGCCGGGACGTTGTGCACCCTGCATAATCTGCATTTCATGGTGGGGTTGGTGGACCGGATTCGCG
AGTCCATGGAGCAGGGGCGCTTCTATGAGTTCCGGGAGGAGTTCATGGGGCGCTACTACGGCCCGACGTGGCGGGAGCGG
CTGGGCTAG

Upstream 100 bases:

>100_bases
ACGGCGCGGCAGCCGGGAGTGCGGGAATCGGCGGTGTGGGATGGGGAATCGGCGGCGCGGAAAGGGGCGCCGGCGGAAGA
GGAAGATAAAATAGGGCGCC

Downstream 100 bases:

>100_bases
CGTTTGCCGGGGTTCCGTGGGCTGGCGGGGCGGCGAGTGCGCGGCATGGCCGCGCAGGGCGTGGCGGGTGCCTATGGCTC
GGCAAGCCGAGCCACCCCAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MADNQENTSADAGAGAADLSFEVRTRLRDSESASAGAGAEGSAGAAASAGAGAEGSAGGSAGAGAGAGGSARLNGSAGAL
GRTGVIKTPHGNIHTPAFIPVATKATVKTLTPEQVRSTGAQAMLSNAYHLYLQPGPDIVDEGGGLAQFENWHGPTYTDSG
GFQVMSLGVGYKKVLAMDTKGRSEADIIQQQKKRMAVVDEDGVDFRSVIDGSKHRFTPEVSMQIQHQLGADIMFAFDELT
TLVNTRSYQEQSVARTHRWARRCLAEHQRLTDLRTHRPLQSLWGVVQGAQYEDLRRQAARGLVQLSEEAEAQGNRGFGGF
GIGGALEKENLGTIVRWVAEELPEDKPRHLLGISEPDDLFTAIEAGADTFDCVAPTRLGRRGGVYTLDGRLNLMGARFKR
DFSPIDAAVGGYVSENYSRAYIHHLLKAKEYLAGTLCTLHNLHFMVGLVDRIRESMEQGRFYEFREEFMGRYYGPTWRER
LG

Sequences:

>Translated_482_residues
MADNQENTSADAGAGAADLSFEVRTRLRDSESASAGAGAEGSAGAAASAGAGAEGSAGGSAGAGAGAGGSARLNGSAGAL
GRTGVIKTPHGNIHTPAFIPVATKATVKTLTPEQVRSTGAQAMLSNAYHLYLQPGPDIVDEGGGLAQFENWHGPTYTDSG
GFQVMSLGVGYKKVLAMDTKGRSEADIIQQQKKRMAVVDEDGVDFRSVIDGSKHRFTPEVSMQIQHQLGADIMFAFDELT
TLVNTRSYQEQSVARTHRWARRCLAEHQRLTDLRTHRPLQSLWGVVQGAQYEDLRRQAARGLVQLSEEAEAQGNRGFGGF
GIGGALEKENLGTIVRWVAEELPEDKPRHLLGISEPDDLFTAIEAGADTFDCVAPTRLGRRGGVYTLDGRLNLMGARFKR
DFSPIDAAVGGYVSENYSRAYIHHLLKAKEYLAGTLCTLHNLHFMVGLVDRIRESMEQGRFYEFREEFMGRYYGPTWRER
LG
>Mature_481_residues
ADNQENTSADAGAGAADLSFEVRTRLRDSESASAGAGAEGSAGAAASAGAGAEGSAGGSAGAGAGAGGSARLNGSAGALG
RTGVIKTPHGNIHTPAFIPVATKATVKTLTPEQVRSTGAQAMLSNAYHLYLQPGPDIVDEGGGLAQFENWHGPTYTDSGG
FQVMSLGVGYKKVLAMDTKGRSEADIIQQQKKRMAVVDEDGVDFRSVIDGSKHRFTPEVSMQIQHQLGADIMFAFDELTT
LVNTRSYQEQSVARTHRWARRCLAEHQRLTDLRTHRPLQSLWGVVQGAQYEDLRRQAARGLVQLSEEAEAQGNRGFGGFG
IGGALEKENLGTIVRWVAEELPEDKPRHLLGISEPDDLFTAIEAGADTFDCVAPTRLGRRGGVYTLDGRLNLMGARFKRD
FSPIDAAVGGYVSENYSRAYIHHLLKAKEYLAGTLCTLHNLHFMVGLVDRIRESMEQGRFYEFREEFMGRYYGPTWRERL
G

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=402, Percent_Identity=35.0746268656716, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1786607, Length=393, Percent_Identity=37.6590330788804, Blast_Score=269, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17544680, Length=401, Percent_Identity=32.1695760598504, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI19920510, Length=407, Percent_Identity=33.4152334152334, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 51998; Mature: 51866

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNQENTSADAGAGAADLSFEVRTRLRDSESASAGAGAEGSAGAAASAGAGAEGSAGGS
CCCCCCCCCCCCCCCCHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGAGAGAGGSARLNGSAGALGRTGVIKTPHGNIHTPAFIPVATKATVKTLTPEQVRSTGA
CCCCCCCCCCEEECCCCCCCCCCCEEECCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHH
QAMLSNAYHLYLQPGPDIVDEGGGLAQFENWHGPTYTDSGGFQVMSLGVGYKKVLAMDTK
HHHHCCCEEEEECCCCCHHCCCCCEECCCCCCCCCCCCCCCCEEEEECCCHHHHEEECCC
GRSEADIIQQQKKRMAVVDEDGVDFRSVIDGSKHRFTPEVSMQIQHQLGADIMFAFDELT
CCCHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
TLVNTRSYQEQSVARTHRWARRCLAEHQRLTDLRTHRPLQSLWGVVQGAQYEDLRRQAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
GLVQLSEEAEAQGNRGFGGFGIGGALEKENLGTIVRWVAEELPEDKPRHLLGISEPDDLF
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHH
TAIEAGADTFDCVAPTRLGRRGGVYTLDGRLNLMGARFKRDFSPIDAAVGGYVSENYSRA
HHHHCCCCCCHHCCCHHCCCCCCEEEECCCEEEHHHHHHCCCCHHHHHHCCHHHCCCHHH
YIHHLLKAKEYLAGTLCTLHNLHFMVGLVDRIRESMEQGRFYEFREEFMGRYYGPTWRER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHH
LG
CC
>Mature Secondary Structure 
ADNQENTSADAGAGAADLSFEVRTRLRDSESASAGAGAEGSAGAAASAGAGAEGSAGGS
CCCCCCCCCCCCCCCHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGAGAGAGGSARLNGSAGALGRTGVIKTPHGNIHTPAFIPVATKATVKTLTPEQVRSTGA
CCCCCCCCCCEEECCCCCCCCCCCEEECCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHH
QAMLSNAYHLYLQPGPDIVDEGGGLAQFENWHGPTYTDSGGFQVMSLGVGYKKVLAMDTK
HHHHCCCEEEEECCCCCHHCCCCCEECCCCCCCCCCCCCCCCEEEEECCCHHHHEEECCC
GRSEADIIQQQKKRMAVVDEDGVDFRSVIDGSKHRFTPEVSMQIQHQLGADIMFAFDELT
CCCHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
TLVNTRSYQEQSVARTHRWARRCLAEHQRLTDLRTHRPLQSLWGVVQGAQYEDLRRQAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
GLVQLSEEAEAQGNRGFGGFGIGGALEKENLGTIVRWVAEELPEDKPRHLLGISEPDDLF
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHH
TAIEAGADTFDCVAPTRLGRRGGVYTLDGRLNLMGARFKRDFSPIDAAVGGYVSENYSRA
HHHHCCCCCCHHCCCHHCCCCCCEEEECCCEEEHHHHHHCCCCHHHHHHCCHHHCCCHHH
YIHHLLKAKEYLAGTLCTLHNLHFMVGLVDRIRESMEQGRFYEFREEFMGRYYGPTWRER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHH
LG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA