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The map label for this gene is pbp1B [H]

Identifier: 68537072

GI number: 68537072

Start: 2302595

End: 2304850

Strand: Direct

Name: pbp1B [H]

Synonym: jk1977

Alternate gene names: 68537072

Gene position: 2302595-2304850 (Clockwise)

Preceding gene: 68537067

Following gene: 68537073

Centisome position: 93.51

GC content: 63.61

Gene sequence:

>2256_bases
ATGGTGATAGCCGTGGAAGCACCGAAGGCATTTCTCAAACTTTTCCTCGCCATCCTGGCCGGTGGTGTGCTGCTGGCCGC
CGCCATCCTGCCCTTTGCGGGCGCGGGCGGCTGGGTGGTCAACGCCAGCACGGACGCGATGAACTCCAATGTGCAGGACA
TCTCCGAGAACAACACGGTGCCACTGCGCTCGCGGATCACCGACCGGGATGGCAAGACGATCGCCTGGGTATACAACCAG
AATCGCACCGAAGTGCCCTCGGACAAGATCTCTCAGGCGATGAAGGACAGCATCGTTGCCATCGAGGATCGGCGCTTTTA
CCAGCACAACGGCGTGGACATCCGCGGCACCATGCGCGCGGCGGCGGCAAACGTCTCCAGCGGCGGGGTCTCGGAAGGCG
CATCCACGATCAACCAGCAGTACGTGAAGAACTACCTGTGGCTGATCGAGGCAGACAACGAGGACGAGGCCAGCCAGGCC
ATCGAAACCTCGCTTCCGCGTAAGCTGCGCGAGATGAAAATGGCCGGCGACCTGGACAAGAAGCTAACTAAGGACGAGAT
CCTGACGCGCTACCTAAACCTGGTCTCCTTCGGCAACAGCGCCTTTGGCGTGCAGACCGCGGCGCAGACCTACTTCGGCA
AGAACGCGGCGGACCTAAATGACAATCAGTCGGCCTTCCTGGCCGGAATCGTGCAGTCCACCAGCGCACTGAACCCGTAC
ACCAACCCGGAGGGCGCGAAGCAGCGGCGCGATGCCGTGCTGCAGGCGCGCGTGAACGCGGGCAGCCTGTCACAGGCCAA
AGCGGACGAGCTCGCGAGCAAGCCGCTGGGCGTGCTGAAGGAGCCGAAGGTTCCCGCCAACGGCTGTATCGGTGCCGGCG
GCTCCGGGTTCTTCTGCGATTACGTGCTGGAGTACCTGGACGAGAAGGGAATCTCCAAGGACGAGGTAGCCAAGGGCGGC
TACACGATCCGCACCACGCTGGATGCGCAGGCGCAGAAGCATGCGGAAACGGCTGCCCGCAGCAAGGTTTCGCCGGATCA
GGAGGGGGTATCCGCCGCGACGAACTACATCACCCCCAAGGATGGCAAGCACCAGGTGGTGGCCATGGCCACCTCGCGCA
AGTATGGCCTTAGCCAAGACGAGCGGGAATCCGTGCTGCCTCTGCCCCACTCGCTGCAGGGACACGGCGCCGGTTCCGTG
TTCAAGATCTTCGCAGCCGCTGCAGCGCTGGAAGACGGCATGGGCCTCAACACCAACCTCGCCGTGCCCACCCGCGCCGA
GGTTGAAGGCATGGGTGCAGGCGGCGCGCCCGGCTGCCCACCAAACAAGTACTGCGTGGAAAATGCAGGCTCCTACAAGC
CCAACATGACTTTGAAGGAAGCGCTGGCGACCAGTCCGAACACCCCCTTCATCGAGATGGCGGAGAAGCTAGGCATGGAG
CGCATTACGGACATTGCCATCAAGCTGGGTCTGCGCTCCTACAAGAACGAGGGCAGCTTCGACGGCAAGCACTCCGTAGA
GGAATACGTCAAGGACAACAACCTTGGCTCCTTCGTGCTGGGCCCCACGGCCGTCGACCCGCTGGAGCTTTCCAACGTGG
CCGCCAGCCTGGCCGACCACGGCCGCTGGTGCGAACCCACGCCCGTGCTGTCCGTAAAGGACCGCAATGGCAAGAAGGTA
AAAATGGAGAAGGCGGACTGCGAGCAGGCCATCGACAAAAAGATCGCCGATGCGCTGGCCAACGGTCTGGGCAGCGACGT
GCAGCAGGGCACTGCCTCTGCTTCCGCCCGCGCGACCGGCTGGAGCGGACCGCTGTCCGCCAAGACGGGTACGACGGAAA
CCAGCTTTTCCGCGGCGTTCCTGGGCTTCACCTCCGGTTGGGCTGGATCCTCCTACATCTTTAACGACGGAGGCACGGCG
AAGAACCTGTGCACCGCGCCTGTGCGCCAATGCGGCAACGGCAACCTCTACGGCGGTAACGAGCCCGCACAGATTTTCTT
CGAGGCCTCGAAGCCGATGATCAAGAAGTACGGTGGCGGCAAGCTGCCCTCCTACGACAAGAAGTACGACGCAGGCACAA
ACCCCGGCAAGTGGGGAGCGCCCGCATCCACCGGCGAAACGGGTGGTGGCGCCTCGAGTGGCGGCAACTCCGCACCCGCC
CCGCAGGCACCACAGGGCCAAGGGGAGCTGGACCTCCCGCCGGAGATCCGCCGCGGCCTCGATGACCTCGATGACTTTCT
GAACCAGCTCCGCTAG

Upstream 100 bases:

>100_bases
GTGCACAAAACTGCAAAGGGGGTGATCACCCCCGGTGGCTGACGTGAGGGCGTCACAAAGAAAGGGCAAAAAGGCTAAAG
GGTTGTCCGGGGCGTGGGTT

Downstream 100 bases:

>100_bases
CGCATTAGCTCCGCCGCGCGTGACGCACGGCGGGTATGTCCCGCCCACGGGCGAAACGATACCCTGGGGAACGTGAAGAA
CAATCGTCGGAACTCCTCAG

Product: putative penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MVIAVEAPKAFLKLFLAILAGGVLLAAAILPFAGAGGWVVNASTDAMNSNVQDISENNTVPLRSRITDRDGKTIAWVYNQ
NRTEVPSDKISQAMKDSIVAIEDRRFYQHNGVDIRGTMRAAAANVSSGGVSEGASTINQQYVKNYLWLIEADNEDEASQA
IETSLPRKLREMKMAGDLDKKLTKDEILTRYLNLVSFGNSAFGVQTAAQTYFGKNAADLNDNQSAFLAGIVQSTSALNPY
TNPEGAKQRRDAVLQARVNAGSLSQAKADELASKPLGVLKEPKVPANGCIGAGGSGFFCDYVLEYLDEKGISKDEVAKGG
YTIRTTLDAQAQKHAETAARSKVSPDQEGVSAATNYITPKDGKHQVVAMATSRKYGLSQDERESVLPLPHSLQGHGAGSV
FKIFAAAAALEDGMGLNTNLAVPTRAEVEGMGAGGAPGCPPNKYCVENAGSYKPNMTLKEALATSPNTPFIEMAEKLGME
RITDIAIKLGLRSYKNEGSFDGKHSVEEYVKDNNLGSFVLGPTAVDPLELSNVAASLADHGRWCEPTPVLSVKDRNGKKV
KMEKADCEQAIDKKIADALANGLGSDVQQGTASASARATGWSGPLSAKTGTTETSFSAAFLGFTSGWAGSSYIFNDGGTA
KNLCTAPVRQCGNGNLYGGNEPAQIFFEASKPMIKKYGGGKLPSYDKKYDAGTNPGKWGAPASTGETGGGASSGGNSAPA
PQAPQGQGELDLPPEIRRGLDDLDDFLNQLR

Sequences:

>Translated_751_residues
MVIAVEAPKAFLKLFLAILAGGVLLAAAILPFAGAGGWVVNASTDAMNSNVQDISENNTVPLRSRITDRDGKTIAWVYNQ
NRTEVPSDKISQAMKDSIVAIEDRRFYQHNGVDIRGTMRAAAANVSSGGVSEGASTINQQYVKNYLWLIEADNEDEASQA
IETSLPRKLREMKMAGDLDKKLTKDEILTRYLNLVSFGNSAFGVQTAAQTYFGKNAADLNDNQSAFLAGIVQSTSALNPY
TNPEGAKQRRDAVLQARVNAGSLSQAKADELASKPLGVLKEPKVPANGCIGAGGSGFFCDYVLEYLDEKGISKDEVAKGG
YTIRTTLDAQAQKHAETAARSKVSPDQEGVSAATNYITPKDGKHQVVAMATSRKYGLSQDERESVLPLPHSLQGHGAGSV
FKIFAAAAALEDGMGLNTNLAVPTRAEVEGMGAGGAPGCPPNKYCVENAGSYKPNMTLKEALATSPNTPFIEMAEKLGME
RITDIAIKLGLRSYKNEGSFDGKHSVEEYVKDNNLGSFVLGPTAVDPLELSNVAASLADHGRWCEPTPVLSVKDRNGKKV
KMEKADCEQAIDKKIADALANGLGSDVQQGTASASARATGWSGPLSAKTGTTETSFSAAFLGFTSGWAGSSYIFNDGGTA
KNLCTAPVRQCGNGNLYGGNEPAQIFFEASKPMIKKYGGGKLPSYDKKYDAGTNPGKWGAPASTGETGGGASSGGNSAPA
PQAPQGQGELDLPPEIRRGLDDLDDFLNQLR
>Mature_751_residues
MVIAVEAPKAFLKLFLAILAGGVLLAAAILPFAGAGGWVVNASTDAMNSNVQDISENNTVPLRSRITDRDGKTIAWVYNQ
NRTEVPSDKISQAMKDSIVAIEDRRFYQHNGVDIRGTMRAAAANVSSGGVSEGASTINQQYVKNYLWLIEADNEDEASQA
IETSLPRKLREMKMAGDLDKKLTKDEILTRYLNLVSFGNSAFGVQTAAQTYFGKNAADLNDNQSAFLAGIVQSTSALNPY
TNPEGAKQRRDAVLQARVNAGSLSQAKADELASKPLGVLKEPKVPANGCIGAGGSGFFCDYVLEYLDEKGISKDEVAKGG
YTIRTTLDAQAQKHAETAARSKVSPDQEGVSAATNYITPKDGKHQVVAMATSRKYGLSQDERESVLPLPHSLQGHGAGSV
FKIFAAAAALEDGMGLNTNLAVPTRAEVEGMGAGGAPGCPPNKYCVENAGSYKPNMTLKEALATSPNTPFIEMAEKLGME
RITDIAIKLGLRSYKNEGSFDGKHSVEEYVKDNNLGSFVLGPTAVDPLELSNVAASLADHGRWCEPTPVLSVKDRNGKKV
KMEKADCEQAIDKKIADALANGLGSDVQQGTASASARATGWSGPLSAKTGTTETSFSAAFLGFTSGWAGSSYIFNDGGTA
KNLCTAPVRQCGNGNLYGGNEPAQIFFEASKPMIKKYGGGKLPSYDKKYDAGTNPGKWGAPASTGETGGGASSGGNSAPA
PQAPQGQGELDLPPEIRRGLDDLDDFLNQLR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=337, Percent_Identity=31.1572700296736, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786343, Length=571, Percent_Identity=28.7215411558669, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788867, Length=453, Percent_Identity=25.60706401766, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1789601, Length=199, Percent_Identity=31.1557788944724, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 79308; Mature: 79308

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIAVEAPKAFLKLFLAILAGGVLLAAAILPFAGAGGWVVNASTDAMNSNVQDISENNTV
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCHHHHCCCCCC
PLRSRITDRDGKTIAWVYNQNRTEVPSDKISQAMKDSIVAIEDRRFYQHNGVDIRGTMRA
CHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCHHHHCCCEEEEHHHHH
AAANVSSGGVSEGASTINQQYVKNYLWLIEADNEDEASQAIETSLPRKLREMKMAGDLDK
HHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
KLTKDEILTRYLNLVSFGNSAFGVQTAAQTYFGKNAADLNDNQSAFLAGIVQSTSALNPY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
TNPEGAKQRRDAVLQARVNAGSLSQAKADELASKPLGVLKEPKVPANGCIGAGGSGFFCD
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
YVLEYLDEKGISKDEVAKGGYTIRTTLDAQAQKHAETAARSKVSPDQEGVSAATNYITPK
HHHHHHHHCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
DGKHQVVAMATSRKYGLSQDERESVLPLPHSLQGHGAGSVFKIFAAAAALEDGMGLNTNL
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
AVPTRAEVEGMGAGGAPGCPPNKYCVENAGSYKPNMTLKEALATSPNTPFIEMAEKLGME
CCCCCHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCHH
RITDIAIKLGLRSYKNEGSFDGKHSVEEYVKDNNLGSFVLGPTAVDPLELSNVAASLADH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHC
GRWCEPTPVLSVKDRNGKKVKMEKADCEQAIDKKIADALANGLGSDVQQGTASASARATG
CCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
WSGPLSAKTGTTETSFSAAFLGFTSGWAGSSYIFNDGGTAKNLCTAPVRQCGNGNLYGGN
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCC
EPAQIFFEASKPMIKKYGGGKLPSYDKKYDAGTNPGKWGAPASTGETGGGASSGGNSAPA
CCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQAPQGQGELDLPPEIRRGLDDLDDFLNQLR
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MVIAVEAPKAFLKLFLAILAGGVLLAAAILPFAGAGGWVVNASTDAMNSNVQDISENNTV
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCHHHHCCCCCC
PLRSRITDRDGKTIAWVYNQNRTEVPSDKISQAMKDSIVAIEDRRFYQHNGVDIRGTMRA
CHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCHHHHCCCEEEEHHHHH
AAANVSSGGVSEGASTINQQYVKNYLWLIEADNEDEASQAIETSLPRKLREMKMAGDLDK
HHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
KLTKDEILTRYLNLVSFGNSAFGVQTAAQTYFGKNAADLNDNQSAFLAGIVQSTSALNPY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
TNPEGAKQRRDAVLQARVNAGSLSQAKADELASKPLGVLKEPKVPANGCIGAGGSGFFCD
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
YVLEYLDEKGISKDEVAKGGYTIRTTLDAQAQKHAETAARSKVSPDQEGVSAATNYITPK
HHHHHHHHCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
DGKHQVVAMATSRKYGLSQDERESVLPLPHSLQGHGAGSVFKIFAAAAALEDGMGLNTNL
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
AVPTRAEVEGMGAGGAPGCPPNKYCVENAGSYKPNMTLKEALATSPNTPFIEMAEKLGME
CCCCCHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCHH
RITDIAIKLGLRSYKNEGSFDGKHSVEEYVKDNNLGSFVLGPTAVDPLELSNVAASLADH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHC
GRWCEPTPVLSVKDRNGKKVKMEKADCEQAIDKKIADALANGLGSDVQQGTASASARATG
CCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
WSGPLSAKTGTTETSFSAAFLGFTSGWAGSSYIFNDGGTAKNLCTAPVRQCGNGNLYGGN
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCC
EPAQIFFEASKPMIKKYGGGKLPSYDKKYDAGTNPGKWGAPASTGETGGGASSGGNSAPA
CCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQAPQGQGELDLPPEIRRGLDDLDDFLNQLR
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]