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The map label for this gene is fadB2 [H]

Identifier: 68535660

GI number: 68535660

Start: 710590

End: 711444

Strand: Direct

Name: fadB2 [H]

Synonym: jk0588

Alternate gene names: 68535660

Gene position: 710590-711444 (Clockwise)

Preceding gene: 68535659

Following gene: 68535661

Centisome position: 28.86

GC content: 61.52

Gene sequence:

>855_bases
ATGGTTCAGCGTGTAGGTGTTATCGGTGCAGGTCTGATGGGATCCGGCATTGCAGAGGTCGCAGCCAAGGCAGGCAGCGA
CGTACTGGTCTGGGAGGCCAAGCAGGAATTCGCCGATGCAGGTAAGGCCCGCATCGAGAAGTCCCTGGAGAAGGCCGTCA
GCCGCGGCAAACTGTCCGAGGAGGACCGCGACGCGGCACTCGGCCGCCTGACCTTCACCACCAAGCTCGAGGACTTCGCT
GACCGCGAGCTGGTCATGGAGGCCATCATTGAGAACGAGGACATCAAGAAGGATGTCTTCGCCAAGCTGGACGAGATCGT
CGAGGACAAGAAGGCTCCACTGTGCTCCAACACGTCCTCCCTGCCGATCCAGACCATCGCCAGCGCAACTAAGAACCCGG
GCCGCGTGCTGGGCCTGCACTTCTTCAACCCAGTTCCGGTCCTGCCGCTGGTGGAGGTCATCCCGGCGCTGACCACCGAC
GAGGACGTTGTTGAGGTTGCCCAGACCTACGCCACCGAGAAGCTGGGTAAGCAGGCCATCCGCGCGAAGGACCGCTCCGG
CTTCATCGTGAACTTCCTGCTGGTGCCGTACATGCTTTCCGCTGTTCGCATGGTGGAAAACGGCGTGGCAACTCCGGAGG
ACATCGATACCGGCATGAAGCTGGGGGCCAACCACCCGATGGGTCCGCTGACCCTAGCAGACATGGTTGGTCTGGATACC
TGCGCCTTTATCGCAGACGTCATGTACAAGGAGTTCGGTGACCCGAGCTACGCCTGCCCGCCGCTGCTGCGCCGCATGGT
CACCGCAGGTCACACCGGGCGCAAGTCCGGCAAGGGATTTTACGAGTACAATTAA

Upstream 100 bases:

>100_bases
AGTTAGTTAATGCAGTCCAGAAAAAACATTGGCCACAATCGCACCTACAACATCGGTAGGCTGGATCTAGTACGAATAAA
CCTATAAAAAAGGAGTGACC

Downstream 100 bases:

>100_bases
CCCCACAACGCCCGCTGGGTAATTCGAAGGCCGTGACCGCGGCTCGGCCCGGTGGGCGGACCCAACGAAAGGAAAGGTCA
CTACATGTCCATTGACGATC

Product: 3-hydroxybutyryl-CoA dehydrogenase

Products: NA

Alternate protein names: Beta-hydroxybutyryl-CoA dehydrogenase; BHBD [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MVQRVGVIGAGLMGSGIAEVAAKAGSDVLVWEAKQEFADAGKARIEKSLEKAVSRGKLSEEDRDAALGRLTFTTKLEDFA
DRELVMEAIIENEDIKKDVFAKLDEIVEDKKAPLCSNTSSLPIQTIASATKNPGRVLGLHFFNPVPVLPLVEVIPALTTD
EDVVEVAQTYATEKLGKQAIRAKDRSGFIVNFLLVPYMLSAVRMVENGVATPEDIDTGMKLGANHPMGPLTLADMVGLDT
CAFIADVMYKEFGDPSYACPPLLRRMVTAGHTGRKSGKGFYEYN

Sequences:

>Translated_284_residues
MVQRVGVIGAGLMGSGIAEVAAKAGSDVLVWEAKQEFADAGKARIEKSLEKAVSRGKLSEEDRDAALGRLTFTTKLEDFA
DRELVMEAIIENEDIKKDVFAKLDEIVEDKKAPLCSNTSSLPIQTIASATKNPGRVLGLHFFNPVPVLPLVEVIPALTTD
EDVVEVAQTYATEKLGKQAIRAKDRSGFIVNFLLVPYMLSAVRMVENGVATPEDIDTGMKLGANHPMGPLTLADMVGLDT
CAFIADVMYKEFGDPSYACPPLLRRMVTAGHTGRKSGKGFYEYN
>Mature_284_residues
MVQRVGVIGAGLMGSGIAEVAAKAGSDVLVWEAKQEFADAGKARIEKSLEKAVSRGKLSEEDRDAALGRLTFTTKLEDFA
DRELVMEAIIENEDIKKDVFAKLDEIVEDKKAPLCSNTSSLPIQTIASATKNPGRVLGLHFFNPVPVLPLVEVIPALTTD
EDVVEVAQTYATEKLGKQAIRAKDRSGFIVNFLLVPYMLSAVRMVENGVATPEDIDTGMKLGANHPMGPLTLADMVGLDT
CAFIADVMYKEFGDPSYACPPLLRRMVTAGHTGRKSGKGFYEYN

Specific function: Catalyzes the NAD-dependent dehydration of beta- hydroxybutyryl-CoA to acetoacetyl-CoA in vitro at pH 10. Also catalyzes the reverse reaction albeit in a lower pH range of 5.5- 6.5. The reverse reaction is able to use NADPH as well as NADH [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI296179429, Length=291, Percent_Identity=42.2680412371134, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI296179427, Length=308, Percent_Identity=39.9350649350649, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI20127408, Length=284, Percent_Identity=34.5070422535211, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI68989263, Length=296, Percent_Identity=31.7567567567568, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI261878539, Length=296, Percent_Identity=31.7567567567568, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI115430219, Length=236, Percent_Identity=29.2372881355932, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787661, Length=284, Percent_Identity=41.5492957746479, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI1788682, Length=269, Percent_Identity=36.4312267657993, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790281, Length=251, Percent_Identity=31.8725099601594, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17549919, Length=289, Percent_Identity=41.8685121107266, Blast_Score=213, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17553560, Length=288, Percent_Identity=37.1527777777778, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17563036, Length=289, Percent_Identity=37.3702422145329, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI25144276, Length=284, Percent_Identity=33.4507042253521, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17508951, Length=284, Percent_Identity=33.4507042253521, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17508953, Length=284, Percent_Identity=33.4507042253521, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17558304, Length=283, Percent_Identity=32.1554770318021, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71985923, Length=288, Percent_Identity=30.2083333333333, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71985930, Length=286, Percent_Identity=29.020979020979, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI193203623, Length=196, Percent_Identity=31.6326530612245, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533614, Length=196, Percent_Identity=31.6326530612245, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24583077, Length=285, Percent_Identity=35.7894736842105, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24583079, Length=285, Percent_Identity=35.7894736842105, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19921000, Length=285, Percent_Identity=35.7894736842105, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45549573, Length=241, Percent_Identity=28.2157676348548, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129993, Length=257, Percent_Identity=24.9027237354086, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022694
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N [H]

EC number: =1.1.1.157 [H]

Molecular weight: Translated: 30739; Mature: 30739

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQRVGVIGAGLMGSGIAEVAAKAGSDVLVWEAKQEFADAGKARIEKSLEKAVSRGKLSE
CCCCCCHHCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EDRDAALGRLTFTTKLEDFADRELVMEAIIENEDIKKDVFAKLDEIVEDKKAPLCSNTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
LPIQTIASATKNPGRVLGLHFFNPVPVLPLVEVIPALTTDEDVVEVAQTYATEKLGKQAI
CCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RAKDRSGFIVNFLLVPYMLSAVRMVENGVATPEDIDTGMKLGANHPMGPLTLADMVGLDT
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
CAFIADVMYKEFGDPSYACPPLLRRMVTAGHTGRKSGKGFYEYN
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVQRVGVIGAGLMGSGIAEVAAKAGSDVLVWEAKQEFADAGKARIEKSLEKAVSRGKLSE
CCCCCCHHCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EDRDAALGRLTFTTKLEDFADRELVMEAIIENEDIKKDVFAKLDEIVEDKKAPLCSNTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
LPIQTIASATKNPGRVLGLHFFNPVPVLPLVEVIPALTTDEDVVEVAQTYATEKLGKQAI
CCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RAKDRSGFIVNFLLVPYMLSAVRMVENGVATPEDIDTGMKLGANHPMGPLTLADMVGLDT
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
CAFIADVMYKEFGDPSYACPPLLRRMVTAGHTGRKSGKGFYEYN
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]