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The map label for this gene is proB [H]

Identifier: 68535635

GI number: 68535635

Start: 683185

End: 684528

Strand: Direct

Name: proB [H]

Synonym: jk0563

Alternate gene names: 68535635

Gene position: 683185-684528 (Clockwise)

Preceding gene: 68535634

Following gene: 68535637

Centisome position: 27.74

GC content: 64.43

Gene sequence:

>1344_bases
ATGTCATCCTCCGATTCCGACATTGCAGACGAAAACGAGTTCAGTTCAGCCCAGTCCGCGTTTAGCTACGCAGGACCGGG
GGAGATGACTCCAGAATTAGCCTCGCCCGTGCCTACCTACGGGCAAAGGCCCGTGCATGAGCCAGGGCCAGTAGTGCCTG
CGACTTCCATCGAGTTTCCGGAGCCGGAAGCAGACGAAGACGATCCAGCGATGGGGCATGATTCCGATGTTCGTGCTGAC
ATCGCCCACGCCCGCCGCCTGGTGGTGAAGATCGGCTCTTCCTCCCTGACCGACGACGATGGTCGTGTGGACCCGAACCA
GATTGACATGATCGCCGACGCGCTGGAAGCGCGCATGGCGCGCGACACCGACCTCATCGTCGTCTCCTCTGGTGCTGTGG
CCTGTGGCATGGGCCCACTGGAGCTCGCCCAGCGCCCCACCGACCTGGCTACCAAGCAGGCCGCCGCGTCTGTCGGCCAG
GTGCTGTTGGCCCAGGAGTGGGCGCGTAGCTTCGCCCGTTATGGTCGTACTATCGGCCAGGTTCTTCTTACTGCTTCGGA
CGCCGCAGAACGCGACCGCGCACGTAATGCCCAGCGCACCATTGACCGCTTGCGCCAGCTGAAGGCCGTGCCGATTGTCA
ACGAGAACGATACGGTCGCTACCTCTGAGATGCGCTTCGGCGATAACGACCGCCTGGCCGCTTTGGTGAGCCACCTGGCC
TTCGCGGATGCTTGCGTGCTGCTATCCGACGTGGACGGCCTGTACGACCGCAACCCGGCCGAGCCGGGCGCCCAGTTCAT
CGCCGAGGTGAAGAGTTCCAAGGACCTGAAGGGCGTGGCTGCCGGCGACGGCGGTCGCCTGGGCACCGGTGGTATGGCGG
CGAAGGTATCGGCTGCCCGCCTGGCCTCCCGCGCGGGTGTGCCGGTGCTACTGACTTCCACGGAGAACATCGGCGCGGCC
CTGGATACCGCGGAGGTCGGAACCTGCTTCTGGCCGGATCAGGACCGCCTGAGCGCTTGGAAGTTCTGGGTGCTCTACGC
TGCGGATTCGCATGGCCAGCTGCACCTGGATGCCGGCGCGGTGCACGCAGTGACGGAGAATCACAAGTCCCTGCTGTCGG
TGGGGATCACCAAGGTCGAGGGCGATTTCTCCCAGCGCGACGTGGTGGACCTGGTCGGGCCAGACGGCAACATCGTCGGC
CGTGGCGAGGTGGCATACGATTCCGCCATGCTGCACGACCTGATTGGCCAGTCGACTTCCGACCTGCCGGAGTTTGCCCG
CAGGCCGGTGGTGCACGCCGACTATATGTCCCATTACTCTAACCGGGCGCAGCTAAAGGGTTAG

Upstream 100 bases:

>100_bases
TTTCGGCGATGGCGAGGTCGCAGAGCGCGAACGTTGGCAGGGGTAGCCAGGTTGTACCAAACGGCACAAGGCCGGGGAGT
GGGCTGGCATACTGGAGAGT

Downstream 100 bases:

>100_bases
TAGGGGTGTGATCCCTTGGCCCGGAGGGGCTGAAGCCGCGTTAGCTGCGCGGCGGTGCCGTTGTGTGGCGAAGCAAGCCC
CTACATGGACTTCGCATTAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSSSDSDIADENEFSSAQSAFSYAGPGEMTPELASPVPTYGQRPVHEPGPVVPATSIEFPEPEADEDDPAMGHDSDVRAD
IAHARRLVVKIGSSSLTDDDGRVDPNQIDMIADALEARMARDTDLIVVSSGAVACGMGPLELAQRPTDLATKQAAASVGQ
VLLAQEWARSFARYGRTIGQVLLTASDAAERDRARNAQRTIDRLRQLKAVPIVNENDTVATSEMRFGDNDRLAALVSHLA
FADACVLLSDVDGLYDRNPAEPGAQFIAEVKSSKDLKGVAAGDGGRLGTGGMAAKVSAARLASRAGVPVLLTSTENIGAA
LDTAEVGTCFWPDQDRLSAWKFWVLYAADSHGQLHLDAGAVHAVTENHKSLLSVGITKVEGDFSQRDVVDLVGPDGNIVG
RGEVAYDSAMLHDLIGQSTSDLPEFARRPVVHADYMSHYSNRAQLKG

Sequences:

>Translated_447_residues
MSSSDSDIADENEFSSAQSAFSYAGPGEMTPELASPVPTYGQRPVHEPGPVVPATSIEFPEPEADEDDPAMGHDSDVRAD
IAHARRLVVKIGSSSLTDDDGRVDPNQIDMIADALEARMARDTDLIVVSSGAVACGMGPLELAQRPTDLATKQAAASVGQ
VLLAQEWARSFARYGRTIGQVLLTASDAAERDRARNAQRTIDRLRQLKAVPIVNENDTVATSEMRFGDNDRLAALVSHLA
FADACVLLSDVDGLYDRNPAEPGAQFIAEVKSSKDLKGVAAGDGGRLGTGGMAAKVSAARLASRAGVPVLLTSTENIGAA
LDTAEVGTCFWPDQDRLSAWKFWVLYAADSHGQLHLDAGAVHAVTENHKSLLSVGITKVEGDFSQRDVVDLVGPDGNIVG
RGEVAYDSAMLHDLIGQSTSDLPEFARRPVVHADYMSHYSNRAQLKG
>Mature_446_residues
SSSDSDIADENEFSSAQSAFSYAGPGEMTPELASPVPTYGQRPVHEPGPVVPATSIEFPEPEADEDDPAMGHDSDVRADI
AHARRLVVKIGSSSLTDDDGRVDPNQIDMIADALEARMARDTDLIVVSSGAVACGMGPLELAQRPTDLATKQAAASVGQV
LLAQEWARSFARYGRTIGQVLLTASDAAERDRARNAQRTIDRLRQLKAVPIVNENDTVATSEMRFGDNDRLAALVSHLAF
ADACVLLSDVDGLYDRNPAEPGAQFIAEVKSSKDLKGVAAGDGGRLGTGGMAAKVSAARLASRAGVPVLLTSTENIGAAL
DTAEVGTCFWPDQDRLSAWKFWVLYAADSHGQLHLDAGAVHAVTENHKSLLSVGITKVEGDFSQRDVVDLVGPDGNIVGR
GEVAYDSAMLHDLIGQSTSDLPEFARRPVVHADYMSHYSNRAQLKG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=291, Percent_Identity=28.5223367697594, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI62912457, Length=289, Percent_Identity=29.0657439446367, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1786437, Length=359, Percent_Identity=36.2116991643454, Blast_Score=195, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17569969, Length=275, Percent_Identity=33.0909090909091, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17569967, Length=275, Percent_Identity=33.0909090909091, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=424, Percent_Identity=32.5471698113208, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321822, Length=410, Percent_Identity=30.2439024390244, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI21357643, Length=329, Percent_Identity=32.8267477203647, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 47473; Mature: 47342

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSDSDIADENEFSSAQSAFSYAGPGEMTPELASPVPTYGQRPVHEPGPVVPATSIEFP
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EPEADEDDPAMGHDSDVRADIAHARRLVVKIGSSSLTDDDGRVDPNQIDMIADALEARMA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHC
RDTDLIVVSSGAVACGMGPLELAQRPTDLATKQAAASVGQVLLAQEWARSFARYGRTIGQ
CCCCEEEEECCCCEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLTASDAAERDRARNAQRTIDRLRQLKAVPIVNENDTVATSEMRFGDNDRLAALVSHLA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEHHCCCCCCHHHHHHHHHHH
FADACVLLSDVDGLYDRNPAEPGAQFIAEVKSSKDLKGVAAGDGGRLGTGGMAAKVSAAR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHH
LASRAGVPVLLTSTENIGAALDTAEVGTCFWPDQDRLSAWKFWVLYAADSHGQLHLDAGA
HHHHCCCCEEEECCCCCCCCCCHHCCCEEECCCCHHHCEEEEEEEEEECCCCEEEECCCC
VHAVTENHKSLLSVGITKVEGDFSQRDVVDLVGPDGNIVGRGEVAYDSAMLHDLIGQSTS
EEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHCCCCC
DLPEFARRPVVHADYMSHYSNRAQLKG
CCHHHHHCCCEEHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSSDSDIADENEFSSAQSAFSYAGPGEMTPELASPVPTYGQRPVHEPGPVVPATSIEFP
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EPEADEDDPAMGHDSDVRADIAHARRLVVKIGSSSLTDDDGRVDPNQIDMIADALEARMA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHC
RDTDLIVVSSGAVACGMGPLELAQRPTDLATKQAAASVGQVLLAQEWARSFARYGRTIGQ
CCCCEEEEECCCCEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLTASDAAERDRARNAQRTIDRLRQLKAVPIVNENDTVATSEMRFGDNDRLAALVSHLA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEHHCCCCCCHHHHHHHHHHH
FADACVLLSDVDGLYDRNPAEPGAQFIAEVKSSKDLKGVAAGDGGRLGTGGMAAKVSAAR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHH
LASRAGVPVLLTSTENIGAALDTAEVGTCFWPDQDRLSAWKFWVLYAADSHGQLHLDAGA
HHHHCCCCEEEECCCCCCCCCCHHCCCEEECCCCHHHCEEEEEEEEEECCCCEEEECCCC
VHAVTENHKSLLSVGITKVEGDFSQRDVVDLVGPDGNIVGRGEVAYDSAMLHDLIGQSTS
EEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHCCCCC
DLPEFARRPVVHADYMSHYSNRAQLKG
CCHHHHHCCCEEHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA