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The map label for this gene is tig

Identifier: 68535615

GI number: 68535615

Start: 658708

End: 660288

Strand: Direct

Name: tig

Synonym: jk0543

Alternate gene names: 68535615

Gene position: 658708-660288 (Clockwise)

Preceding gene: 68535613

Following gene: 68535616

Centisome position: 26.75

GC content: 60.85

Gene sequence:

>1581_bases
GTGAAGAGCTCTGTAGAAAAGCTGAGCGCCACCCGCACCAAGCTGACGATTGAGGTTCCATTCGAGGAGCTTAAGCCAGA
GTTCGACAAGGCCTACGCAACCCTCGCGCAGCAGGTAAACATGCCGGGCTTCCGCAAGGGCAAGGTACCGGCGAAGATTC
TGGAGGCTCGCCTGGGCCGTGGCGTTGTCCTGGATCAGGTCATCAACGAGATGCTGCCTTCTCGCTACAGCCAGGCTGTC
GAGGAGAACGACGTGAAGGCCCTGGGCCAGCCGGAGATCGAAATCGCCGAGCTGGAGGACGGCAAGCACATCACCTTCAC
CGCCGAGGTCGACGTTCGCCCGGAGATCGAGGTTCCGGACTTCTCCGCCATCTCCGTTGAGGTTGCACCGCTGAAGGCCG
ACGACGAAGCCATCGACGCGGAGCTGAAGAACCTGCAGGCTCGCTTCGGCACCCTGAAGCCGGCTGACCGCGCAGTGAAG
AAGGGCGACTTCGTCTCCATCGACCTGTCCGCCACCATCGACGGCGAGACCGTTGACGAGGCCACCACCGAGGGCCTGTC
CCACGAGGTCGGCAACGATTCCCTGATCGAGGGCCTGGATGACGCGCTGGTCGGTATGAAGGAGGGCGAGGAGTCCACTT
TCACCTCCAAGCTGGTTGCCGGCGAGCACGCTGACGAAGAGGCTGAGGTTACCGTCAAGCTCGGTTCCGTCAAGGAGCGC
GAGCTGCCGGAGCTGGACGACGACTTCGCGCAGCTGGCCAGCGAGTTCGATACCCTGGACGAGCTGAAGGAGTCCCTGAA
GGGGCAGGTCGAGCAGCAGCTGAAGAACACCCAGGCTGGCGAGATCCGCGATAAGGTTCTGGCCGAGGCTCTGGAGCAGA
CCGAGTTCGAGCTGCCCGAGGGTGTTGTTAAGGAGCAGGTCGACGCGCAGATCCAGCAGATCATCCAGCAGTTCGGTGGC
GACGAGAAGGTCTTCGAGTCCATGCTGGCTGCCCAGGACATGACTCGCGAGAAGTTCGAAGAGGATGCACGCAGCTCCGC
TGAGGACTCCGTGCGTACCCAGCTGTTCCTGGATGCTGTTGCGGACAAGGAGCAGCCGGAGGTTTCCCAGGAAGAGCTGA
TGGATCACATCGCCTTCACTGCAAACCAGTACGGCATGGATCCGAACCAGTTCATCATGCAGCTGCAGCAGGCCGGCCAG
CTGGGCAGCCTGTTCGCTGACGTCCGCCGCGGCAAGGCACTGGCGCTGAACATCGCCCGTACCTCCGTGAAGGACACTGA
CGGTGCGGACGTTGATCCGAAGGAGTACTTCGGCGACGTTGAGGCTGAGGGTGACAAGGCAGACAAGGCCGAGACTGATA
AGGCCGAGGAGAAGCCGAAGAAGGCTCCTGCCAAGAAGTCCACCACGAAGAAGTCGACTGCTAAGAAGAGCACGGCCAAG
AAGTCGACCGCCAAGAAGTCCACTGCTAAGAAGAGCACGGCCAAGAAGTCGACCACCAAGAAGGCTACGAAGTCTACGGC
GAAGAAGTCCACTGCCAAGAAGACCACTGCAAAGAAGGCAGCGGAGAAGAAGGAAGATTAA

Upstream 100 bases:

>100_bases
CGATGGTTCTATAAAATTGAGCACCGACCGTAAAAGCGCTGCGAGATGAACGAGAGACCGCGGTGCCAAAGAAGCTATTA
ATACACAGGAGTGTGCATTC

Downstream 100 bases:

>100_bases
GAGTCGTCCTACACCGCCGGGACCTCCACGCGAGGTTCTGGCGGTTTTCTTTTTGTCCCGCCTTTCCCGTTACCCGCCCC
GGTGCGCAGCCATATCGTGC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKSSVEKLSATRTKLTIEVPFEELKPEFDKAYATLAQQVNMPGFRKGKVPAKILEARLGRGVVLDQVINEMLPSRYSQAV
EENDVKALGQPEIEIAELEDGKHITFTAEVDVRPEIEVPDFSAISVEVAPLKADDEAIDAELKNLQARFGTLKPADRAVK
KGDFVSIDLSATIDGETVDEATTEGLSHEVGNDSLIEGLDDALVGMKEGEESTFTSKLVAGEHADEEAEVTVKLGSVKER
ELPELDDDFAQLASEFDTLDELKESLKGQVEQQLKNTQAGEIRDKVLAEALEQTEFELPEGVVKEQVDAQIQQIIQQFGG
DEKVFESMLAAQDMTREKFEEDARSSAEDSVRTQLFLDAVADKEQPEVSQEELMDHIAFTANQYGMDPNQFIMQLQQAGQ
LGSLFADVRRGKALALNIARTSVKDTDGADVDPKEYFGDVEAEGDKADKAETDKAEEKPKKAPAKKSTTKKSTAKKSTAK
KSTAKKSTAKKSTAKKSTTKKATKSTAKKSTAKKTTAKKAAEKKED

Sequences:

>Translated_526_residues
MKSSVEKLSATRTKLTIEVPFEELKPEFDKAYATLAQQVNMPGFRKGKVPAKILEARLGRGVVLDQVINEMLPSRYSQAV
EENDVKALGQPEIEIAELEDGKHITFTAEVDVRPEIEVPDFSAISVEVAPLKADDEAIDAELKNLQARFGTLKPADRAVK
KGDFVSIDLSATIDGETVDEATTEGLSHEVGNDSLIEGLDDALVGMKEGEESTFTSKLVAGEHADEEAEVTVKLGSVKER
ELPELDDDFAQLASEFDTLDELKESLKGQVEQQLKNTQAGEIRDKVLAEALEQTEFELPEGVVKEQVDAQIQQIIQQFGG
DEKVFESMLAAQDMTREKFEEDARSSAEDSVRTQLFLDAVADKEQPEVSQEELMDHIAFTANQYGMDPNQFIMQLQQAGQ
LGSLFADVRRGKALALNIARTSVKDTDGADVDPKEYFGDVEAEGDKADKAETDKAEEKPKKAPAKKSTTKKSTAKKSTAK
KSTAKKSTAKKSTAKKSTTKKATKSTAKKSTAKKTTAKKAAEKKED
>Mature_526_residues
MKSSVEKLSATRTKLTIEVPFEELKPEFDKAYATLAQQVNMPGFRKGKVPAKILEARLGRGVVLDQVINEMLPSRYSQAV
EENDVKALGQPEIEIAELEDGKHITFTAEVDVRPEIEVPDFSAISVEVAPLKADDEAIDAELKNLQARFGTLKPADRAVK
KGDFVSIDLSATIDGETVDEATTEGLSHEVGNDSLIEGLDDALVGMKEGEESTFTSKLVAGEHADEEAEVTVKLGSVKER
ELPELDDDFAQLASEFDTLDELKESLKGQVEQQLKNTQAGEIRDKVLAEALEQTEFELPEGVVKEQVDAQIQQIIQQFGG
DEKVFESMLAAQDMTREKFEEDARSSAEDSVRTQLFLDAVADKEQPEVSQEELMDHIAFTANQYGMDPNQFIMQLQQAGQ
LGSLFADVRRGKALALNIARTSVKDTDGADVDPKEYFGDVEAEGDKADKAETDKAEEKPKKAPAKKSTTKKSTAKKSTAK
KSTAKKSTAKKSTAKKSTTKKATKSTAKKSTAKKTTAKKAAEKKED

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=400, Percent_Identity=28.25, Blast_Score=142, Evalue=7e-35,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CORJK (Q4JWV5)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250320.1
- HSSP:   P0A850
- ProteinModelPortal:   Q4JWV5
- STRING:   Q4JWV5
- GeneID:   3433266
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0543
- NMPDR:   fig|306537.3.peg.539
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   TERENIE
- ProtClustDB:   PRK01490
- BioCyc:   CJEI306537:JK0543-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 57569; Mature: 57569

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSVEKLSATRTKLTIEVPFEELKPEFDKAYATLAQQVNMPGFRKGKVPAKILEARLGR
CCCHHHHHHHHHEEEEEECCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
GVVLDQVINEMLPSRYSQAVEENDVKALGQPEIEIAELEDGKHITFTAEVDVRPEIEVPD
CHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCC
FSAISVEVAPLKADDEAIDAELKNLQARFGTLKPADRAVKKGDFVSIDLSATIDGETVDE
CCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEEECCCHHHH
ATTEGLSHEVGNDSLIEGLDDALVGMKEGEESTFTSKLVAGEHADEEAEVTVKLGSVKER
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
ELPELDDDFAQLASEFDTLDELKESLKGQVEQQLKNTQAGEIRDKVLAEALEQTEFELPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCH
GVVKEQVDAQIQQIIQQFGGDEKVFESMLAAQDMTREKFEEDARSSAEDSVRTQLFLDAV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ADKEQPEVSQEELMDHIAFTANQYGMDPNQFIMQLQQAGQLGSLFADVRRGKALALNIAR
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEHH
TSVKDTDGADVDPKEYFGDVEAEGDKADKAETDKAEEKPKKAPAKKSTTKKSTAKKSTAK
HCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
KSTAKKSTAKKSTAKKSTTKKATKSTAKKSTAKKTTAKKAAEKKED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSSVEKLSATRTKLTIEVPFEELKPEFDKAYATLAQQVNMPGFRKGKVPAKILEARLGR
CCCHHHHHHHHHEEEEEECCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
GVVLDQVINEMLPSRYSQAVEENDVKALGQPEIEIAELEDGKHITFTAEVDVRPEIEVPD
CHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCC
FSAISVEVAPLKADDEAIDAELKNLQARFGTLKPADRAVKKGDFVSIDLSATIDGETVDE
CCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEEECCCHHHH
ATTEGLSHEVGNDSLIEGLDDALVGMKEGEESTFTSKLVAGEHADEEAEVTVKLGSVKER
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
ELPELDDDFAQLASEFDTLDELKESLKGQVEQQLKNTQAGEIRDKVLAEALEQTEFELPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCH
GVVKEQVDAQIQQIIQQFGGDEKVFESMLAAQDMTREKFEEDARSSAEDSVRTQLFLDAV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ADKEQPEVSQEELMDHIAFTANQYGMDPNQFIMQLQQAGQLGSLFADVRRGKALALNIAR
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEHH
TSVKDTDGADVDPKEYFGDVEAEGDKADKAETDKAEEKPKKAPAKKSTTKKSTAKKSTAK
HCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
KSTAKKSTAKKSTAKKSTTKKATKSTAKKSTAKKTTAKKAAEKKED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA