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The map label for this gene is mesJ [H]

Identifier: 68535341

GI number: 68535341

Start: 339560

End: 340612

Strand: Direct

Name: mesJ [H]

Synonym: jk0276

Alternate gene names: 68535341

Gene position: 339560-340612 (Clockwise)

Preceding gene: 68535340

Following gene: 68535342

Centisome position: 13.79

GC content: 69.52

Gene sequence:

>1053_bases
ATGAGTGTTCAGTCGCAGATCCGGGCCTGGCTGCGAGAGCTGGAAACCGGGGAAGCCAGCGGCGGCGGAACCAGTGAAAC
CAGCCCCGAGTCCGCAGCGCGCGCGGCACAGGTTCGCGCCGGGGTGGCAGCGGGACTTTCCGGCGGGGCGGACTCCCTGG
CACTGGTGCACGGCCTGCTGCGCTGCGGGGTGAAGGTACACGCGGTAGTGGTGGACCATCAGCTGCAGGAAGGCTCCGCG
CAGGTGGCGCGGCAGGCCGCGGATACCGCACTGTCCCTAGGCGCGACGGCGGAGATCCGCACCGTCGACGTGGAAGGCCC
AGGGGAGGGCCCGGCGCGCCAGGCGCGCTATGAAGCCCTGGGCGCGGCGGCCGCCGGGCGGCCGCTCCTGGTAGCTCACA
CGGCGGATGACGACGCAGAAGGGATGCTCATCGGTCTGGTCCGCGGCTCCGGGGCGGAAGCTATCGCCGGGATGCGATCT
GTGCACACCGACCACGCGGCCGTGGCCGCGGGGGCCGCGTGGCTCGGCCGTCCGCTGCTGAACTGCACCCGCGCGGAGAC
AGAGGCCGAATGCCGCGCCGCCGGTCTTAACTGGTGGGAGGATCCGCACAACTCCAGCGATGCCTACCTGCGCAGCCGCA
TCCGCACCCACGTGCTGCCGTACCTGGAAGATGTGCTCGGGGAGCACATCGGTGCGAACATGGCCCGCACCGCGCGGATG
CTTCGCGAAGACGTCGAGCTGCTCGACGAGCTCGCTGGGCGGGAGCTTCAGGCGGCGGAGGAGGGGGCGTCATTAAACTG
CACCGAACTACAACAGCAACCAGCGGCACTGCGGCGCAGGGTGCTCAAACAATGGCTGGCAGATAAGGCCGGCCCCCTCA
CGAGCGCGCACCTCAACGCGATAGACGCGCTGGTCATCGCATGGAAGGGGCAAGGAGGGGCGGCCGTGCCGTGGCCGCAG
AGCCCACCTGCATTGAACGAGCAACGGCGCACCCACCGACTCGTCGTGCAACGCCGCGATGGTTACCTACAGTTAGCGAC
TGTCCCCAGGTAG

Upstream 100 bases:

>100_bases
AGCGCTGACGTTTGCGTTCCTCTCCAATGAGTCCGATCCCAACTCCGCCCGCCCGGCGCTGGATCGGTTGGCGAATGCGG
TGCGTTCAGCCGAGTAAGCC

Downstream 100 bases:

>100_bases
CAGCTGCGCGCTAGAATGCCAGGCAGGCACAAAACGGCGAAGCCAATGCAGCGGAGCCAAGACAGCGAAGCCACACTGCG
AAAGCACTGCAAAACAAACG

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSVQSQIRAWLRELETGEASGGGTSETSPESAARAAQVRAGVAAGLSGGADSLALVHGLLRCGVKVHAVVVDHQLQEGSA
QVARQAADTALSLGATAEIRTVDVEGPGEGPARQARYEALGAAAAGRPLLVAHTADDDAEGMLIGLVRGSGAEAIAGMRS
VHTDHAAVAAGAAWLGRPLLNCTRAETEAECRAAGLNWWEDPHNSSDAYLRSRIRTHVLPYLEDVLGEHIGANMARTARM
LREDVELLDELAGRELQAAEEGASLNCTELQQQPAALRRRVLKQWLADKAGPLTSAHLNAIDALVIAWKGQGGAAVPWPQ
SPPALNEQRRTHRLVVQRRDGYLQLATVPR

Sequences:

>Translated_350_residues
MSVQSQIRAWLRELETGEASGGGTSETSPESAARAAQVRAGVAAGLSGGADSLALVHGLLRCGVKVHAVVVDHQLQEGSA
QVARQAADTALSLGATAEIRTVDVEGPGEGPARQARYEALGAAAAGRPLLVAHTADDDAEGMLIGLVRGSGAEAIAGMRS
VHTDHAAVAAGAAWLGRPLLNCTRAETEAECRAAGLNWWEDPHNSSDAYLRSRIRTHVLPYLEDVLGEHIGANMARTARM
LREDVELLDELAGRELQAAEEGASLNCTELQQQPAALRRRVLKQWLADKAGPLTSAHLNAIDALVIAWKGQGGAAVPWPQ
SPPALNEQRRTHRLVVQRRDGYLQLATVPR
>Mature_349_residues
SVQSQIRAWLRELETGEASGGGTSETSPESAARAAQVRAGVAAGLSGGADSLALVHGLLRCGVKVHAVVVDHQLQEGSAQ
VARQAADTALSLGATAEIRTVDVEGPGEGPARQARYEALGAAAAGRPLLVAHTADDDAEGMLIGLVRGSGAEAIAGMRSV
HTDHAAVAAGAAWLGRPLLNCTRAETEAECRAAGLNWWEDPHNSSDAYLRSRIRTHVLPYLEDVLGEHIGANMARTARML
REDVELLDELAGRELQAAEEGASLNCTELQQQPAALRRRVLKQWLADKAGPLTSAHLNAIDALVIAWKGQGGAAVPWPQS
PPALNEQRRTHRLVVQRRDGYLQLATVPR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=278, Percent_Identity=29.8561151079137, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946 [H]

EC number: NA

Molecular weight: Translated: 37011; Mature: 36880

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQSQIRAWLRELETGEASGGGTSETSPESAARAAQVRAGVAAGLSGGADSLALVHGLL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RCGVKVHAVVVDHQLQEGSAQVARQAADTALSLGATAEIRTVDVEGPGEGPARQARYEAL
HCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHH
GAAAAGRPLLVAHTADDDAEGMLIGLVRGSGAEAIAGMRSVHTDHAAVAAGAAWLGRPLL
HHHHCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NCTRAETEAECRAAGLNWWEDPHNSSDAYLRSRIRTHVLPYLEDVLGEHIGANMARTARM
HHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LREDVELLDELAGRELQAAEEGASLNCTELQQQPAALRRRVLKQWLADKAGPLTSAHLNA
HHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IDALVIAWKGQGGAAVPWPQSPPALNEQRRTHRLVVQRRDGYLQLATVPR
HEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCEEEEEECCC
>Mature Secondary Structure 
SVQSQIRAWLRELETGEASGGGTSETSPESAARAAQVRAGVAAGLSGGADSLALVHGLL
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RCGVKVHAVVVDHQLQEGSAQVARQAADTALSLGATAEIRTVDVEGPGEGPARQARYEAL
HCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHH
GAAAAGRPLLVAHTADDDAEGMLIGLVRGSGAEAIAGMRSVHTDHAAVAAGAAWLGRPLL
HHHHCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NCTRAETEAECRAAGLNWWEDPHNSSDAYLRSRIRTHVLPYLEDVLGEHIGANMARTARM
HHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LREDVELLDELAGRELQAAEEGASLNCTELQQQPAALRRRVLKQWLADKAGPLTSAHLNA
HHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IDALVIAWKGQGGAAVPWPQSPPALNEQRRTHRLVVQRRDGYLQLATVPR
HEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA