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The map label for this gene is pbp4 [H]

Identifier: 68535340

GI number: 68535340

Start: 338222

End: 339556

Strand: Direct

Name: pbp4 [H]

Synonym: jk0275

Alternate gene names: 68535340

Gene position: 338222-339556 (Clockwise)

Preceding gene: 68535338

Following gene: 68535341

Centisome position: 13.73

GC content: 63.3

Gene sequence:

>1335_bases
GTGAGTGAGCAGAAGTCCCTGAAAGTATGGCTGTGGAGCATCCTCGCCTTCGTGGTCGTTGTTGCGGTCGTGGTGGGGGC
TGCTGTGATGTGGTCGCTAAACGGACGCTATGAGGTACAAGCCGCAGAGCCGTTGGCGGAACCCTCCCCGGTGATGCTTC
CGCCCGAAGGGGACGTGCCGGACGTGCAGGCCGCAGTTCAGCGCGCTGCAGCGGATCCGGCTCTGGGGGTGCTGTCCGCC
TCCGTCACCGATGTTGAGACCGGCGAGGTGCTGTGGGCCAGGGATGAGAACCGGAAGCTTACCCCGGCTTCTGCCACCAA
GCTGGCTACTGCTGCTGCCGCGCTGCTGGCGCTAGACGGTGCCGCTACGTGGAAGACCGAGGTCCTGCAGGATCCTTCCG
ACCCCGGCACGCTGTATCTGCGCAGCGATGGCGATGTCACTCTTTCCCGCGACGGCAATGGCTTTTTTAACGACGCTGCC
TCGATCGCCACGCTGGCCGAGGACGTAAAGAAGGAGTTGGGGGGTCAGCCCGTCAACAAGATTGTCGTGGACAACTCCAT
TCGTTCCGAAGACCTTTTCAACAAGTCGTGGGATCGCGCGGATATCGATGGCGGTAACGTCGCGAACCTCGATGCTGTGA
TGTTGGACTCCGGCCGGCTCAACCCTGGGGAGTCCTATTCTGCCCGCTCCCACACCCCTGGTGAGGATGTGGGGCGCGCC
CTAGCCGATGCGCTCGGCGCTAATGCGGAGGTCGGTGTTTCTTCGGAAATGACGCCAGATCTCAGCAACCAAAGCCCTAT
TGCACAGGTCGAGTCTGCACCGCTGAAGGTTCGCCTGCGCGACATGCTGGTGCACAGCGACAACATGCTGGCCGAGGCCG
TTGGCCGGGAGATTGCTAAGGCGAAAGGCGCACCACAGACTTTTGAGGGCGCCACGAAGGCGACTCTGGACGTGCTGAAG
GAACACGGCATCGATACCGAGGACGCGGTGCTTTTCGACAACTCGGGTATGAGCGACAAGAACCGCCTGACCGCCCACGA
ACTGGATGCCCTGCTGGCCAATGAGCAGCTGCGGCCTTTGCTGGACATGCTGCCGGTCTCCAGTGTGGAGGGCTCCCTGG
AGGCGCGCTATGGTTCCGGCTCCGGCTCGGAGGACTCCGCCGGCTGGGTGCGCGCCAAGACCGGCACCCTCTCCGGCGTG
AACGCCCTGGCCGGGACGGTTATGACGGATTCGGGGCGAGCGCTGACGTTTGCGTTCCTCTCCAATGAGTCCGATCCCAA
CTCCGCCCGCCCGGCGCTGGATCGGTTGGCGAATGCGGTGCGTTCAGCCGAGTAA

Upstream 100 bases:

>100_bases
CTTGTTGCGGGAGCCCTTCGGGATTTCAATGGTTACTTCGATTTTTTTGCTCATGGGCACCATTCTAACTGAGTGCCTTT
TTTCGGCTAGGGTGAAACGA

Downstream 100 bases:

>100_bases
GCCATGAGTGTTCAGTCGCAGATCCGGGCCTGGCTGCGAGAGCTGGAAACCGGGGAAGCCAGCGGCGGCGGAACCAGTGA
AACCAGCCCCGAGTCCGCAG

Product: putative penicillin-binding protein 4

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSEQKSLKVWLWSILAFVVVVAVVVGAAVMWSLNGRYEVQAAEPLAEPSPVMLPPEGDVPDVQAAVQRAAADPALGVLSA
SVTDVETGEVLWARDENRKLTPASATKLATAAAALLALDGAATWKTEVLQDPSDPGTLYLRSDGDVTLSRDGNGFFNDAA
SIATLAEDVKKELGGQPVNKIVVDNSIRSEDLFNKSWDRADIDGGNVANLDAVMLDSGRLNPGESYSARSHTPGEDVGRA
LADALGANAEVGVSSEMTPDLSNQSPIAQVESAPLKVRLRDMLVHSDNMLAEAVGREIAKAKGAPQTFEGATKATLDVLK
EHGIDTEDAVLFDNSGMSDKNRLTAHELDALLANEQLRPLLDMLPVSSVEGSLEARYGSGSGSEDSAGWVRAKTGTLSGV
NALAGTVMTDSGRALTFAFLSNESDPNSARPALDRLANAVRSAE

Sequences:

>Translated_444_residues
MSEQKSLKVWLWSILAFVVVVAVVVGAAVMWSLNGRYEVQAAEPLAEPSPVMLPPEGDVPDVQAAVQRAAADPALGVLSA
SVTDVETGEVLWARDENRKLTPASATKLATAAAALLALDGAATWKTEVLQDPSDPGTLYLRSDGDVTLSRDGNGFFNDAA
SIATLAEDVKKELGGQPVNKIVVDNSIRSEDLFNKSWDRADIDGGNVANLDAVMLDSGRLNPGESYSARSHTPGEDVGRA
LADALGANAEVGVSSEMTPDLSNQSPIAQVESAPLKVRLRDMLVHSDNMLAEAVGREIAKAKGAPQTFEGATKATLDVLK
EHGIDTEDAVLFDNSGMSDKNRLTAHELDALLANEQLRPLLDMLPVSSVEGSLEARYGSGSGSEDSAGWVRAKTGTLSGV
NALAGTVMTDSGRALTFAFLSNESDPNSARPALDRLANAVRSAE
>Mature_443_residues
SEQKSLKVWLWSILAFVVVVAVVVGAAVMWSLNGRYEVQAAEPLAEPSPVMLPPEGDVPDVQAAVQRAAADPALGVLSAS
VTDVETGEVLWARDENRKLTPASATKLATAAAALLALDGAATWKTEVLQDPSDPGTLYLRSDGDVTLSRDGNGFFNDAAS
IATLAEDVKKELGGQPVNKIVVDNSIRSEDLFNKSWDRADIDGGNVANLDAVMLDSGRLNPGESYSARSHTPGEDVGRAL
ADALGANAEVGVSSEMTPDLSNQSPIAQVESAPLKVRLRDMLVHSDNMLAEAVGREIAKAKGAPQTFEGATKATLDVLKE
HGIDTEDAVLFDNSGMSDKNRLTAHELDALLANEQLRPLLDMLPVSSVEGSLEARYGSGSGSEDSAGWVRAKTGTLSGVN
ALAGTVMTDSGRALTFAFLSNESDPNSARPALDRLANAVRSAE

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=157, Percent_Identity=34.3949044585987, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46629; Mature: 46498

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKSLKVWLWSILAFVVVVAVVVGAAVMWSLNGRYEVQAAEPLAEPSPVMLPPEGDVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCEEECCCCCCC
DVQAAVQRAAADPALGVLSASVTDVETGEVLWARDENRKLTPASATKLATAAAALLALDG
HHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
AATWKTEVLQDPSDPGTLYLRSDGDVTLSRDGNGFFNDAASIATLAEDVKKELGGQPVNK
CCHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IVVDNSIRSEDLFNKSWDRADIDGGNVANLDAVMLDSGRLNPGESYSARSHTPGEDVGRA
EEECCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
LADALGANAEVGVSSEMTPDLSNQSPIAQVESAPLKVRLRDMLVHSDNMLAEAVGREIAK
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHH
AKGAPQTFEGATKATLDVLKEHGIDTEDAVLFDNSGMSDKNRLTAHELDALLANEQLRPL
HCCCCCHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LDMLPVSSVEGSLEARYGSGSGSEDSAGWVRAKTGTLSGVNALAGTVMTDSGRALTFAFL
HHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHEEECCCCEEEEEEE
SNESDPNSARPALDRLANAVRSAE
CCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEQKSLKVWLWSILAFVVVVAVVVGAAVMWSLNGRYEVQAAEPLAEPSPVMLPPEGDVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCEEECCCCCCC
DVQAAVQRAAADPALGVLSASVTDVETGEVLWARDENRKLTPASATKLATAAAALLALDG
HHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
AATWKTEVLQDPSDPGTLYLRSDGDVTLSRDGNGFFNDAASIATLAEDVKKELGGQPVNK
CCHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IVVDNSIRSEDLFNKSWDRADIDGGNVANLDAVMLDSGRLNPGESYSARSHTPGEDVGRA
EEECCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
LADALGANAEVGVSSEMTPDLSNQSPIAQVESAPLKVRLRDMLVHSDNMLAEAVGREIAK
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHH
AKGAPQTFEGATKATLDVLKEHGIDTEDAVLFDNSGMSDKNRLTAHELDALLANEQLRPL
HCCCCCHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LDMLPVSSVEGSLEARYGSGSGSEDSAGWVRAKTGTLSGVNALAGTVMTDSGRALTFAFL
HHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHEEECCCCEEEEEEE
SNESDPNSARPALDRLANAVRSAE
CCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]