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The map label for this gene is cysI [H]

Identifier: 68535308

GI number: 68535308

Start: 300303

End: 301997

Strand: Direct

Name: cysI [H]

Synonym: jk0243

Alternate gene names: 68535308

Gene position: 300303-301997 (Clockwise)

Preceding gene: 68535305

Following gene: 68535309

Centisome position: 12.2

GC content: 62.89

Gene sequence:

>1695_bases
ATGAGCACCGGAACGAGCACCGAGGAAAAGCCAGCGCGCCCGCGCCGCAAGAAGCAGCGCAAGCCACAGGGACAATGGGC
TGTGGACGGCCGCGAGCCGCTCAACGACGACGAGCGGGTAAAGCAGGAAGATCCTGGAATTAGTGTGATGCAGCGCATCC
GCGACATCTATTCCAAGGAGGGCTTCGACTCGATCCCCGCTGAGGACCTCGCGCCGCGCTTCAAGTGGGTTGGCATGTAT
ACCCAGCGCAAGCAGGGGCTCGACGGTGAGAAGACGTCGACCCTGACCAACACGGAGCTGCAGGACAACTACTTTATGAT
GCGCATCCGCCTGGATGGCGGCGTGGTTAGCTCCGAGGGGCTACGCACCATCGGCGAGATCTCCGAAAAGTACGCGCGCA
ATACCGCCGACTTCACCGACCGCCAGAACGTGCAGCTGCACTGGATACGCATCGAAGATGTGCCGACTATCTGGGACGAG
CTGGCGAAGGTCGGCCTGGATTCCTTCTTTGGATGCGGCGACGTCCCGCGCGTCATTCTCGGCTCCCCGGTCGCGGGCAT
TGCCAAGGATGAAATCATCGACGGCCGCCCGGCCATCGAAAAGATCAAAAAGGAACTTCTGCCGGACCCTGAGTTCGGCA
ACCTGCCGCGTAAGTTCAAGTCCGCTATCTCTGGGCACGCCCGCCAGGACGTAACCCATGAGATCCAGGACCTCGCCTTC
ATTGGCTCCGAGCACCCGGAGCACGGCCCCGGCTTCGATGTCTGGGTCGGCGGTGGTCTGTCTACCAATCCGATGCTCGC
TCAGCGCCTGGGCGTGTGGGTTCCGATCGACGAGGTGCCCGAAGTCTGGGCTGGCGTGGTTCGTATCTTCCGCGACTATG
GCTACCGCCGCATGCGCAACCGTGCTCGCCTGAAGTTCCTGGTGGCCGACTGGGGCGTGGAGAAGTTCCGCGAGGTTCTG
GAAAACGAGTACCTCAAGCGCCCACTACTGGACGGTCCGGAGCCGGAAGAGTATCCGGGCTACCGCGACCACGTCGGCCT
GCACGAGCAAAAGGACGGCCGCTTCTACCTGGGTGTTAAGCCCACCGTCGGCCACACCGACGGCAAGCAGCTGCAGCGGC
TGGCCGACCTGGCGGAAAAGCATGGCACCTCCAATATCCGCACCACACCGGACAAGGAGCTTCTGTTCCTGGACCTGGAG
CGCGAGGCCGCGGAGGCTCTCGCTGCGGATCTGGAGGAGCTCGGCCTGTCCGCCAAACCCTCGGCCTTCCGTCGCGACAT
TATCTCCTGCACCGGTCTGGAGTTCTGCAAGCTCGCACACGTCGTCACCAAGCAGCGCGCCATCGCCCTGGTGGATGAGC
TGGAGGATCGCCTGGGTGACCTCGACGTGCCGCTGAAGATTAGCCTCAACGGCTGCCCGAATTCCTGTGCTCGCACTCAG
GTGGCGGATATCGGCCTGTCTGGCAAGATCCTCACTACCGACGAGGGCGAGCGCGTGGAGGGCTTCCAGGTGCACCTCGG
CGGAACCATTGGTCTGGAGCCGCACTTCGGCAAGAAGGTGCGCGGCAACCAGGTCTTCTCCAAGGATCTGGGCGACTATG
TGGTCCGTGTGGTGGAGAACTTCAAGTCTCACCGCACCGAGGGCGAGGAGTTCCGCGATTGGGTTGTCCGCGCCGACGAT
GAGCTGTTGGTGTAG

Upstream 100 bases:

>100_bases
TATAAAAATCAACATTTCGCTTGAATTTGGGCGAGAACCCATCTAGGCTGAACCGGGCAATGTAGACAGACAGATCAGTC
TAGACGGAATTTGAGGCACC

Downstream 100 bases:

>100_bases
TTGATGAGTGTTCCGGATAAAGACTCTCTGCCGAGCGTGAACGAGCAGGTCGGCGGCCGTGTGGGTCACCCGAATGCCCG
CCGCGCGACGGTGAACAACT

Product: sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MSTGTSTEEKPARPRRKKQRKPQGQWAVDGREPLNDDERVKQEDPGISVMQRIRDIYSKEGFDSIPAEDLAPRFKWVGMY
TQRKQGLDGEKTSTLTNTELQDNYFMMRIRLDGGVVSSEGLRTIGEISEKYARNTADFTDRQNVQLHWIRIEDVPTIWDE
LAKVGLDSFFGCGDVPRVILGSPVAGIAKDEIIDGRPAIEKIKKELLPDPEFGNLPRKFKSAISGHARQDVTHEIQDLAF
IGSEHPEHGPGFDVWVGGGLSTNPMLAQRLGVWVPIDEVPEVWAGVVRIFRDYGYRRMRNRARLKFLVADWGVEKFREVL
ENEYLKRPLLDGPEPEEYPGYRDHVGLHEQKDGRFYLGVKPTVGHTDGKQLQRLADLAEKHGTSNIRTTPDKELLFLDLE
REAAEALAADLEELGLSAKPSAFRRDIISCTGLEFCKLAHVVTKQRAIALVDELEDRLGDLDVPLKISLNGCPNSCARTQ
VADIGLSGKILTTDEGERVEGFQVHLGGTIGLEPHFGKKVRGNQVFSKDLGDYVVRVVENFKSHRTEGEEFRDWVVRADD
ELLV

Sequences:

>Translated_564_residues
MSTGTSTEEKPARPRRKKQRKPQGQWAVDGREPLNDDERVKQEDPGISVMQRIRDIYSKEGFDSIPAEDLAPRFKWVGMY
TQRKQGLDGEKTSTLTNTELQDNYFMMRIRLDGGVVSSEGLRTIGEISEKYARNTADFTDRQNVQLHWIRIEDVPTIWDE
LAKVGLDSFFGCGDVPRVILGSPVAGIAKDEIIDGRPAIEKIKKELLPDPEFGNLPRKFKSAISGHARQDVTHEIQDLAF
IGSEHPEHGPGFDVWVGGGLSTNPMLAQRLGVWVPIDEVPEVWAGVVRIFRDYGYRRMRNRARLKFLVADWGVEKFREVL
ENEYLKRPLLDGPEPEEYPGYRDHVGLHEQKDGRFYLGVKPTVGHTDGKQLQRLADLAEKHGTSNIRTTPDKELLFLDLE
REAAEALAADLEELGLSAKPSAFRRDIISCTGLEFCKLAHVVTKQRAIALVDELEDRLGDLDVPLKISLNGCPNSCARTQ
VADIGLSGKILTTDEGERVEGFQVHLGGTIGLEPHFGKKVRGNQVFSKDLGDYVVRVVENFKSHRTEGEEFRDWVVRADD
ELLV
>Mature_563_residues
STGTSTEEKPARPRRKKQRKPQGQWAVDGREPLNDDERVKQEDPGISVMQRIRDIYSKEGFDSIPAEDLAPRFKWVGMYT
QRKQGLDGEKTSTLTNTELQDNYFMMRIRLDGGVVSSEGLRTIGEISEKYARNTADFTDRQNVQLHWIRIEDVPTIWDEL
AKVGLDSFFGCGDVPRVILGSPVAGIAKDEIIDGRPAIEKIKKELLPDPEFGNLPRKFKSAISGHARQDVTHEIQDLAFI
GSEHPEHGPGFDVWVGGGLSTNPMLAQRLGVWVPIDEVPEVWAGVVRIFRDYGYRRMRNRARLKFLVADWGVEKFREVLE
NEYLKRPLLDGPEPEEYPGYRDHVGLHEQKDGRFYLGVKPTVGHTDGKQLQRLADLAEKHGTSNIRTTPDKELLFLDLER
EAAEALAADLEELGLSAKPSAFRRDIISCTGLEFCKLAHVVTKQRAIALVDELEDRLGDLDVPLKISLNGCPNSCARTQV
ADIGLSGKILTTDEGERVEGFQVHLGGTIGLEPHFGKKVRGNQVFSKDLGDYVVRVVENFKSHRTEGEEFRDWVVRADDE
LLV

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=587, Percent_Identity=24.190800681431, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322597, Length=555, Percent_Identity=24.3243243243243, Blast_Score=120, Evalue=6e-28,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 63544; Mature: 63412

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGTSTEEKPARPRRKKQRKPQGQWAVDGREPLNDDERVKQEDPGISVMQRIRDIYSKE
CCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCC
GFDSIPAEDLAPRFKWVGMYTQRKQGLDGEKTSTLTNTELQDNYFMMRIRLDGGVVSSEG
CCCCCCHHHHCCHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCEEEEEEEECCCEECCHH
LRTIGEISEKYARNTADFTDRQNVQLHWIRIEDVPTIWDELAKVGLDSFFGCGDVPRVIL
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHH
GSPVAGIAKDEIIDGRPAIEKIKKELLPDPEFGNLPRKFKSAISGHARQDVTHEIQDLAF
CCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
IGSEHPEHGPGFDVWVGGGLSTNPMLAQRLGVWVPIDEVPEVWAGVVRIFRDYGYRRMRN
HCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RARLKFLVADWGVEKFREVLENEYLKRPLLDGPEPEEYPGYRDHVGLHEQKDGRFYLGVK
HHHEEEEHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEC
PTVGHTDGKQLQRLADLAEKHGTSNIRTTPDKELLFLDLEREAAEALAADLEELGLSAKP
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCC
SAFRRDIISCTGLEFCKLAHVVTKQRAIALVDELEDRLGDLDVPLKISLNGCPNSCARTQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
VADIGLSGKILTTDEGERVEGFQVHLGGTIGLEPHFGKKVRGNQVFSKDLGDYVVRVVEN
HHHCCCCCEEEECCCCCCCCCEEEEECCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FKSHRTEGEEFRDWVVRADDELLV
HHHHCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STGTSTEEKPARPRRKKQRKPQGQWAVDGREPLNDDERVKQEDPGISVMQRIRDIYSKE
CCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCC
GFDSIPAEDLAPRFKWVGMYTQRKQGLDGEKTSTLTNTELQDNYFMMRIRLDGGVVSSEG
CCCCCCHHHHCCHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCEEEEEEEECCCEECCHH
LRTIGEISEKYARNTADFTDRQNVQLHWIRIEDVPTIWDELAKVGLDSFFGCGDVPRVIL
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHH
GSPVAGIAKDEIIDGRPAIEKIKKELLPDPEFGNLPRKFKSAISGHARQDVTHEIQDLAF
CCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
IGSEHPEHGPGFDVWVGGGLSTNPMLAQRLGVWVPIDEVPEVWAGVVRIFRDYGYRRMRN
HCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RARLKFLVADWGVEKFREVLENEYLKRPLLDGPEPEEYPGYRDHVGLHEQKDGRFYLGVK
HHHEEEEHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEC
PTVGHTDGKQLQRLADLAEKHGTSNIRTTPDKELLFLDLEREAAEALAADLEELGLSAKP
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCC
SAFRRDIISCTGLEFCKLAHVVTKQRAIALVDELEDRLGDLDVPLKISLNGCPNSCARTQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
VADIGLSGKILTTDEGERVEGFQVHLGGTIGLEPHFGKKVRGNQVFSKDLGDYVVRVVEN
HHHCCCCCEEEECCCCCCCCCEEEEECCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FKSHRTEGEEFRDWVVRADDELLV
HHHHCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA