Click here to switch to the map view.

The map label for this gene is trmH [C]

Identifier: 68535279

GI number: 68535279

Start: 266567

End: 267250

Strand: Direct

Name: trmH [C]

Synonym: jk0214

Alternate gene names: 68535279

Gene position: 266567-267250 (Clockwise)

Preceding gene: 68535278

Following gene: 68535280

Centisome position: 10.83

GC content: 63.6

Gene sequence:

>684_bases
ATGACGCCCACCTCCCCCGAATCACACGATAAACCCGGACCGACGGAGTGGCTGGAGCATCCCCGTGGGGTGCGTAGCTG
GGAAGAAGAACACCCCGACCAGCCCCGCCCGACGGATGCGCGCTTCGACCCCGAGCTGCTGGATGCCGGGGACACGCGCA
ACGTGGTGGATGCTTACCGGTACTGGAAGCAGTCCGCGATCATTGCGGATATTGATACCCGTCGGCATGCGCTGCACATT
GCCATTGAGAACTTCGAAAACGACTCGAACATTGGCACCGTCGTGCGCACTGCGAATGCCTTCGCGGTGGATACCGTGCA
CATTGTTGGGCGGCGGCGTTGGAACCGGCGCGGGGCGATGGTTACTGACCGCTACCAGCACTTACAGCACCATGCGACGG
TGGGAGAGGTGCTGGAATTCGCCGCCCGGGAAGGGCTAGTTGTGGTGGCGGTGGATAACACACCGGGGTGCGTGCCGCTG
GAAACCGCGGAACTGCCGGAGCGGTGCCTGCTGCTGTTTGGCCAGGAAGGGCCGGGTGTGACCCAGGAGGCGCAGGATGC
TGCGCGGATGACGGTCTCCATTGCTCAGTTCGGTTCGACGCGGTCTATTAATGCGGGTGTAGCAGCGGGGATTGCGATGC
ATGCGTGGATCCGCCAGCACGCGGACCTGTCCCAGGCCTGGTAG

Upstream 100 bases:

>100_bases
GGATCGCGCGACCGGCGCTAAGGATGTTATTGAGGCCGAGGGAGTCGAGTACCGCTCGCTGCTGGGCCTGTCCGACCTGG
GTCTTTCCTAGGTATTTTTG

Downstream 100 bases:

>100_bases
GCGGAGCTGACCTGGGGGTTGGTGGACGGGGCTTACCTGGGGCTGGTTGGCGAGGCTAGGCTTTGCTGCTCTCCGGGCTG
TGTTCTCCTGTAAATGTCGG

Product: putative rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: RNA Methyltransferase; rRNA Methylase; TRNA/RRNA MethyltransferaU; RNA Methyltransferase TrmH Family; 23S RRNA Methyltransferase; TrmH Family RNA Methyltransferase; tRNA Guanosine-2-O-Methyltransferase; RNA Methylase Family Protein; Spou RRNA Methylase Family Protein; Methyltransferase; SPOU RRNA Methylase Family Protein; TRNA/RRNA Methyltransferase Family Protein

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTPTSPESHDKPGPTEWLEHPRGVRSWEEEHPDQPRPTDARFDPELLDAGDTRNVVDAYRYWKQSAIIADIDTRRHALHI
AIENFENDSNIGTVVRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQHLQHHATVGEVLEFAAREGLVVVAVDNTPGCVPL
ETAELPERCLLLFGQEGPGVTQEAQDAARMTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLSQAW

Sequences:

>Translated_227_residues
MTPTSPESHDKPGPTEWLEHPRGVRSWEEEHPDQPRPTDARFDPELLDAGDTRNVVDAYRYWKQSAIIADIDTRRHALHI
AIENFENDSNIGTVVRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQHLQHHATVGEVLEFAAREGLVVVAVDNTPGCVPL
ETAELPERCLLLFGQEGPGVTQEAQDAARMTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLSQAW
>Mature_226_residues
TPTSPESHDKPGPTEWLEHPRGVRSWEEEHPDQPRPTDARFDPELLDAGDTRNVVDAYRYWKQSAIIADIDTRRHALHIA
IENFENDSNIGTVVRTANAFAVDTVHIVGRRRWNRRGAMVTDRYQHLQHHATVGEVLEFAAREGLVVVAVDNTPGCVPLE
TAELPERCLLLFGQEGPGVTQEAQDAARMTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLSQAW

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790083, Length=156, Percent_Identity=26.2820512820513, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 25281; Mature: 25150

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTSPESHDKPGPTEWLEHPRGVRSWEEEHPDQPRPTDARFDPELLDAGDTRNVVDAYR
CCCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHH
YWKQSAIIADIDTRRHALHIAIENFENDSNIGTVVRTANAFAVDTVHIVGRRRWNRRGAM
HHHHCCEEEECCCCCEEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCC
VTDRYQHLQHHATVGEVLEFAAREGLVVVAVDNTPGCVPLETAELPERCLLLFGQEGPGV
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEECCHHHCCHHHHEEECCCCCCC
TQEAQDAARMTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLSQAW
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPTSPESHDKPGPTEWLEHPRGVRSWEEEHPDQPRPTDARFDPELLDAGDTRNVVDAYR
CCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHH
YWKQSAIIADIDTRRHALHIAIENFENDSNIGTVVRTANAFAVDTVHIVGRRRWNRRGAM
HHHHCCEEEECCCCCEEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCC
VTDRYQHLQHHATVGEVLEFAAREGLVVVAVDNTPGCVPLETAELPERCLLLFGQEGPGV
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEECCHHHCCHHHHEEECCCCCCC
TQEAQDAARMTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLSQAW
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA