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The map label for this gene is gcvH

Identifier: 68535276

GI number: 68535276

Start: 263817

End: 264200

Strand: Direct

Name: gcvH

Synonym: jk0211

Alternate gene names: 68535276

Gene position: 263817-264200 (Clockwise)

Preceding gene: 68535275

Following gene: 68535277

Centisome position: 10.71

GC content: 60.68

Gene sequence:

>384_bases
ATGACTGCACTGCCAACTGACTTCCTGTACTCCGAAGAGCACGAGTGGGTTAACACCTCCGCTGTTGTTGAGGGCGAGAC
CGTGCGCGTGGGCATTACCCACATCGCCGCTGAGGCGCTGGGTGACATCGTGTTCGTCGAGCTGCCGGAGGTTGGCTCCG
AGGTTGAGGCCGGCGAGGCTTTCGGCGAGGTTGAGTCCACCAAGTCCGTTTCCGACATCTACGCACCGGTTTCTGGCGAG
GTTGTGGCTGTCAACGAGGCGCTGGAAGACAACGCTGGCCTGATCAACGAAGATCCATACGGTGAGGGCTGGCTGTACGA
GGTCAAGGTGACCGAGGCCGGCGAGCTGATGGAGGCTGAGGCTTACCAGGCGGCTAACGAGTAA

Upstream 100 bases:

>100_bases
GCAAGAAGTAACGAAAAGGGTTGTCTGCCCGCTGGTGTAAGGTTTGGGCAGACAACCACAGAAGTAACCACACAAGGTTT
CACAAGAGAGGCTTAAAACA

Downstream 100 bases:

>100_bases
AACAAGACCCTATGGTCGGTTCCATGTTCGCATTAATCCTCGGCTTGGCCCTGTTGGGCCTTGCCGTGTGGTTACTCCTG
CGCGCAAAAGATAGCACCGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MTALPTDFLYSEEHEWVNTSAVVEGETVRVGITHIAAEALGDIVFVELPEVGSEVEAGEAFGEVESTKSVSDIYAPVSGE
VVAVNEALEDNAGLINEDPYGEGWLYEVKVTEAGELMEAEAYQAANE

Sequences:

>Translated_127_residues
MTALPTDFLYSEEHEWVNTSAVVEGETVRVGITHIAAEALGDIVFVELPEVGSEVEAGEAFGEVESTKSVSDIYAPVSGE
VVAVNEALEDNAGLINEDPYGEGWLYEVKVTEAGELMEAEAYQAANE
>Mature_126_residues
TALPTDFLYSEEHEWVNTSAVVEGETVRVGITHIAAEALGDIVFVELPEVGSEVEAGEAFGEVESTKSVSDIYAPVSGEV
VAVNEALEDNAGLINEDPYGEGWLYEVKVTEAGELMEAEAYQAANE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=45.2991452991453, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=44.4444444444444, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=50.3937007874016, Blast_Score=121, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=108, Percent_Identity=52.7777777777778, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=48.7179487179487, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=109, Percent_Identity=47.7064220183486, Blast_Score=110, Evalue=8e-26,
Organism=Drosophila melanogaster, GI17865652, Length=124, Percent_Identity=41.1290322580645, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CORJK (Q4JXU4)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_249981.1
- HSSP:   P16048
- ProteinModelPortal:   Q4JXU4
- SMR:   Q4JXU4
- STRING:   Q4JXU4
- GeneID:   3433826
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0211
- NMPDR:   fig|306537.3.peg.520
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   CJEI306537:JK0211-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13597; Mature: 13466

Theoretical pI: Translated: 3.44; Mature: 3.44

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALPTDFLYSEEHEWVNTSAVVEGETVRVGITHIAAEALGDIVFVELPEVGSEVEAGEA
CCCCCCCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCHH
FGEVESTKSVSDIYAPVSGEVVAVNEALEDNAGLINEDPYGEGWLYEVKVTEAGELMEAE
HCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
AYQAANE
HHHCCCC
>Mature Secondary Structure 
TALPTDFLYSEEHEWVNTSAVVEGETVRVGITHIAAEALGDIVFVELPEVGSEVEAGEA
CCCCCCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCHH
FGEVESTKSVSDIYAPVSGEVVAVNEALEDNAGLINEDPYGEGWLYEVKVTEAGELMEAE
HCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
AYQAANE
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA