Click here to switch to the map view.

The map label for this gene is gcvT

Identifier: 68535275

GI number: 68535275

Start: 262546

End: 263715

Strand: Direct

Name: gcvT

Synonym: jk0210

Alternate gene names: 68535275

Gene position: 262546-263715 (Clockwise)

Preceding gene: 68535274

Following gene: 68535276

Centisome position: 10.66

GC content: 64.1

Gene sequence:

>1170_bases
ATGACCGAACTTAAGAAGACCGCGCTGCACCTGGTGCACGAGAAGTTGGGCGCGCGATTTACCGACTTCGGCGGCTGGGA
CATGCCTCTGAAGTACAGCAGTGAGCTGGACGAGCACCACGCTGTACGCAATGCCGTGGGCGTATTCGACCTCTCCCACA
TGGGTGAGGTTCGCGTGACCGGCCCGCAGGCAGCGGAGTTCCTGGACCACGCGCTGATTTCGAAGCTGTCGGCAGTGAAG
GTCGGCAAGGCGAAGTACTCGATGATCTGCACCGAATCCGGTGGCATCATCGACGACCTGATCACCTACCGCCTGGGCGA
CAACGAGTTCCTGATCGTGCCGAACGCGGGCAACGTGGACAACGTGGTCTCCGCACTGCAGGGCCGCACCGAGGGCTTTG
ACGTGGAGGTTAACAACGAGTCCGATGCGACCTCCATGATCGCCGTACAGGGGCCCAAGGCCGCGCAGGCGATGCTGGAG
ATCGTGGAGAACGTCGTGGATGCACCCGAGGCATCCGGCGCGGGCGAGACCGTTGCCGAGGCTATCGAGGGGCTGGGTTA
CTACGCGGCATTCAGCGGTGTTGCCGCAGGTCAGCCCGTGCTGGTGGCCCGCACAGGCTATACCGGCGAGGACGGTTTCG
AGCTGATCGTGGCTAACGATGGTGCGGAGACCGTGTGGACCAAGGCTATGGACCAGGCTGCGCAGCTGGGTGGCCTGCCG
TGTGGCCTGGCCTGCCGCGACACCCTGCGCCTGGAGGCTGGCATGCCGCTGTACGGCAACGAGCTATCGCTGAAGCTCAC
CCCGGTCGATGCTGGGCTGGGCATTCTTGCGGCGACGAAGTCTAAGGACTCTTTCGTTGGTCGTGACGCCATCGTTTCCG
CCAAGGAAAAGGGTACCCAGCAGGTACTTATCGGGCTGGCGGGCGAGGGTCGCCGCGCTGCCCGTGGGGGATACGAGGTG
TTTGCCGGTGACGGCGAGAAGGCCATCGGTGCCGTGACCTCCGGTGCACTGTCGCCGACGCTGGGCCACCCGGTGGCATT
GGCATACGTCGCGAAGTCCGCAGTGTCCTCCGGCGCGGCCGCTGAGGGTGCGACCGTGGAGGTAGACATCCGCGGCAAGC
GCTTTGAATACAAGGTTGTGGCGCTGCCGTTCTACTCCCGCGAGAAGTAA

Upstream 100 bases:

>100_bases
TCTCGTGTGCACCTGCCCGCCCCTGGAAGACTTCGCAATTAACGAGGACTAGGGTCCGCTGGAGCTAAGGGCGTCACAAA
GAGAAGAAGGAGAACAAGAC

Downstream 100 bases:

>100_bases
CGCAAGAAGTAACGAAAAGGGTTGTCTGCCCGCTGGTGTAAGGTTTGGGCAGACAACCACAGAAGTAACCACACAAGGTT
TCACAAGAGAGGCTTAAAAC

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTELKKTALHLVHEKLGARFTDFGGWDMPLKYSSELDEHHAVRNAVGVFDLSHMGEVRVTGPQAAEFLDHALISKLSAVK
VGKAKYSMICTESGGIIDDLITYRLGDNEFLIVPNAGNVDNVVSALQGRTEGFDVEVNNESDATSMIAVQGPKAAQAMLE
IVENVVDAPEASGAGETVAEAIEGLGYYAAFSGVAAGQPVLVARTGYTGEDGFELIVANDGAETVWTKAMDQAAQLGGLP
CGLACRDTLRLEAGMPLYGNELSLKLTPVDAGLGILAATKSKDSFVGRDAIVSAKEKGTQQVLIGLAGEGRRAARGGYEV
FAGDGEKAIGAVTSGALSPTLGHPVALAYVAKSAVSSGAAAEGATVEVDIRGKRFEYKVVALPFYSREK

Sequences:

>Translated_389_residues
MTELKKTALHLVHEKLGARFTDFGGWDMPLKYSSELDEHHAVRNAVGVFDLSHMGEVRVTGPQAAEFLDHALISKLSAVK
VGKAKYSMICTESGGIIDDLITYRLGDNEFLIVPNAGNVDNVVSALQGRTEGFDVEVNNESDATSMIAVQGPKAAQAMLE
IVENVVDAPEASGAGETVAEAIEGLGYYAAFSGVAAGQPVLVARTGYTGEDGFELIVANDGAETVWTKAMDQAAQLGGLP
CGLACRDTLRLEAGMPLYGNELSLKLTPVDAGLGILAATKSKDSFVGRDAIVSAKEKGTQQVLIGLAGEGRRAARGGYEV
FAGDGEKAIGAVTSGALSPTLGHPVALAYVAKSAVSSGAAAEGATVEVDIRGKRFEYKVVALPFYSREK
>Mature_388_residues
TELKKTALHLVHEKLGARFTDFGGWDMPLKYSSELDEHHAVRNAVGVFDLSHMGEVRVTGPQAAEFLDHALISKLSAVKV
GKAKYSMICTESGGIIDDLITYRLGDNEFLIVPNAGNVDNVVSALQGRTEGFDVEVNNESDATSMIAVQGPKAAQAMLEI
VENVVDAPEASGAGETVAEAIEGLGYYAAFSGVAAGQPVLVARTGYTGEDGFELIVANDGAETVWTKAMDQAAQLGGLPC
GLACRDTLRLEAGMPLYGNELSLKLTPVDAGLGILAATKSKDSFVGRDAIVSAKEKGTQQVLIGLAGEGRRAARGGYEVF
AGDGEKAIGAVTSGALSPTLGHPVALAYVAKSAVSSGAAAEGATVEVDIRGKRFEYKVVALPFYSREK

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=391, Percent_Identity=29.923273657289, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI257796258, Length=357, Percent_Identity=30.812324929972, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI257796254, Length=386, Percent_Identity=27.979274611399, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI257796256, Length=338, Percent_Identity=29.2899408284024, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI24797151, Length=349, Percent_Identity=24.6418338108883, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI197927446, Length=309, Percent_Identity=25.2427184466019, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI21361378, Length=309, Percent_Identity=25.2427184466019, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789272, Length=387, Percent_Identity=38.7596899224806, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17560118, Length=391, Percent_Identity=31.7135549872123, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71994045, Length=384, Percent_Identity=25.2604166666667, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71994052, Length=384, Percent_Identity=25.2604166666667, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI32563613, Length=367, Percent_Identity=25.6130790190736, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320222, Length=408, Percent_Identity=33.8235294117647, Blast_Score=192, Evalue=8e-50,
Organism=Drosophila melanogaster, GI20129441, Length=405, Percent_Identity=31.358024691358, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI20130091, Length=369, Percent_Identity=24.9322493224932, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_CORJK (Q4JXU5)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_249980.1
- HSSP:   P27248
- ProteinModelPortal:   Q4JXU5
- SMR:   Q4JXU5
- STRING:   Q4JXU5
- GeneID:   3433828
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0210
- NMPDR:   fig|306537.3.peg.519
- eggNOG:   COG0404
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- BioCyc:   CJEI306537:JK0210-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40653; Mature: 40522

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELKKTALHLVHEKLGARFTDFGGWDMPLKYSSELDEHHAVRNAVGVFDLSHMGEVRVT
CCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCEEEC
GPQAAEFLDHALISKLSAVKVGKAKYSMICTESGGIIDDLITYRLGDNEFLIVPNAGNVD
CCHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHEEECCCCEEEEECCCCHH
NVVSALQGRTEGFDVEVNNESDATSMIAVQGPKAAQAMLEIVENVVDAPEASGAGETVAE
HHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AIEGLGYYAAFSGVAAGQPVLVARTGYTGEDGFELIVANDGAETVWTKAMDQAAQLGGLP
HHHCCCHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
CGLACRDTLRLEAGMPLYGNELSLKLTPVDAGLGILAATKSKDSFVGRDAIVSAKEKGTQ
CCHHHCCCEEEECCCCEECCEEEEEEEEECCCCEEEEECCCCCCCCCCHHHEECHHCCCE
QVLIGLAGEGRRAARGGYEVFAGDGEKAIGAVTSGALSPTLGHPVALAYVAKSAVSSGAA
EEEEEECCCCCCHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
AEGATVEVDIRGKRFEYKVVALPFYSREK
CCCCEEEEEECCCEEEEEEEEECCCCCCC
>Mature Secondary Structure 
TELKKTALHLVHEKLGARFTDFGGWDMPLKYSSELDEHHAVRNAVGVFDLSHMGEVRVT
CHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCEEEC
GPQAAEFLDHALISKLSAVKVGKAKYSMICTESGGIIDDLITYRLGDNEFLIVPNAGNVD
CCHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHEEECCCCEEEEECCCCHH
NVVSALQGRTEGFDVEVNNESDATSMIAVQGPKAAQAMLEIVENVVDAPEASGAGETVAE
HHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AIEGLGYYAAFSGVAAGQPVLVARTGYTGEDGFELIVANDGAETVWTKAMDQAAQLGGLP
HHHCCCHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
CGLACRDTLRLEAGMPLYGNELSLKLTPVDAGLGILAATKSKDSFVGRDAIVSAKEKGTQ
CCHHHCCCEEEECCCCEECCEEEEEEEEECCCCEEEEECCCCCCCCCCHHHEECHHCCCE
QVLIGLAGEGRRAARGGYEVFAGDGEKAIGAVTSGALSPTLGHPVALAYVAKSAVSSGAA
EEEEEECCCCCCHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
AEGATVEVDIRGKRFEYKVVALPFYSREK
CCCCEEEEEECCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA