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The map label for this gene is ogt [H]

Identifier: 68535253

GI number: 68535253

Start: 236928

End: 237461

Strand: Direct

Name: ogt [H]

Synonym: jk0188

Alternate gene names: 68535253

Gene position: 236928-237461 (Clockwise)

Preceding gene: 68535252

Following gene: 68535256

Centisome position: 9.62

GC content: 67.6

Gene sequence:

>534_bases
GTGGCAGATCTGACAGGCCAGGAACAGACCGGGGTAGGCGGGGCAGTGCTGAAGTTTGATGCCCTGAAGTCTGAGGTCTT
CCCCAGAAGTGAGCTGTTGGCGCTGGCCACGGAGCGAGGCTTGGTGGCCATAGAGTTTGCACACGTGTGGCAGGGGCGAG
AACTGCCACCTGCCGAGGGCGCGGCCGCCCAGCATTTGGAGGAGGCTGCGAGGCAACTGGGGGAGTACCTCGCGGGGGAG
CGCCGAGAGTTCACCGTGCCCGTGGACTTAAGTGGCGTGACCACTCCCTTCCGGCGCCGGGCGACACAGGCCCTGGCGCA
GATCCCGTATGGGGAGACCATTACGTATGCCCAGCTGGCGGCATTGGCGGGCAATCCGCGCGCCGTGCGCGCTGCGGCGT
CGGCGTGCGCGCGCAACCCACTGCCCATCCTGTACCCCTGCCACCGGGTGATCCGCAGCGACGGGAGGCTCGGTGAGTAC
CGGGGTGGCGCGGAGACTAAGAGGGCGCTGCTGGAGATGGAGCGCGCGCTCTAA

Upstream 100 bases:

>100_bases
AGTACCGGCAGCGTGGGAAGACGGTGGAGTTTGTGGGGATGAACGAGTTCTCCTCGGCCTTCCATACGCGCCTGACGGGC
GAGCTGGGTGGCGAAGGCTA

Downstream 100 bases:

>100_bases
TTTTGCACATTCACCTCCGGGGACCACATGTAGGAACTGTGGGCGTCAGTCTCCCCGCGGCTCATGAGGTAGAGGTCCAA
GATTCGGCTCCAGCCGTAGC

Product: 6-O-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MADLTGQEQTGVGGAVLKFDALKSEVFPRSELLALATERGLVAIEFAHVWQGRELPPAEGAAAQHLEEAARQLGEYLAGE
RREFTVPVDLSGVTTPFRRRATQALAQIPYGETITYAQLAALAGNPRAVRAAASACARNPLPILYPCHRVIRSDGRLGEY
RGGAETKRALLEMERAL

Sequences:

>Translated_177_residues
MADLTGQEQTGVGGAVLKFDALKSEVFPRSELLALATERGLVAIEFAHVWQGRELPPAEGAAAQHLEEAARQLGEYLAGE
RREFTVPVDLSGVTTPFRRRATQALAQIPYGETITYAQLAALAGNPRAVRAAASACARNPLPILYPCHRVIRSDGRLGEY
RGGAETKRALLEMERAL
>Mature_176_residues
ADLTGQEQTGVGGAVLKFDALKSEVFPRSELLALATERGLVAIEFAHVWQGRELPPAEGAAAQHLEEAARQLGEYLAGER
REFTVPVDLSGVTTPFRRRATQALAQIPYGETITYAQLAALAGNPRAVRAAASACARNPLPILYPCHRVIRSDGRLGEYR
GGAETKRALLEMERAL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=77, Percent_Identity=50.6493506493506, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788542, Length=153, Percent_Identity=39.8692810457516, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787596, Length=107, Percent_Identity=41.1214953271028, Blast_Score=74, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=52.4390243902439, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=52.4390243902439, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=34.5679012345679, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19137; Mature: 19006

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLTGQEQTGVGGAVLKFDALKSEVFPRSELLALATERGLVAIEFAHVWQGRELPPAEG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCC
AAAQHLEEAARQLGEYLAGERREFTVPVDLSGVTTPFRRRATQALAQIPYGETITYAQLA
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
ALAGNPRAVRAAASACARNPLPILYPCHRVIRSDGRLGEYRGGAETKRALLEMERAL
HHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADLTGQEQTGVGGAVLKFDALKSEVFPRSELLALATERGLVAIEFAHVWQGRELPPAEG
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCC
AAAQHLEEAARQLGEYLAGERREFTVPVDLSGVTTPFRRRATQALAQIPYGETITYAQLA
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
ALAGNPRAVRAAASACARNPLPILYPCHRVIRSDGRLGEYRGGAETKRALLEMERAL
HHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]