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The map label for this gene is gltD [H]

Identifier: 68535140

GI number: 68535140

Start: 88348

End: 89886

Strand: Direct

Name: gltD [H]

Synonym: jk0077

Alternate gene names: 68535140

Gene position: 88348-89886 (Clockwise)

Preceding gene: 68535139

Following gene: 68535141

Centisome position: 3.59

GC content: 66.21

Gene sequence:

>1539_bases
ATGGCTGATCCACGCGGATTTCTGAAGCACCGTCGCGAGTCGGCGGCACACCGACCCGTCCCGCTGCGCCTGCTGGATTG
GAACGAGGTCTACGAAGACTTCTCCGATGACAAGGTGCAGACCCAGGCCAGCCGTTGCATGGACTGCGGCATCCCGTTCT
GCCACGATGGCTGCCCGCTGGGCAACATCATCCCCGAGTGGAACGACCTGGTGCGTAAGGGCAACTGGCGCGAGGCCTAC
GACCGCCTGCACGCCACCAACAACTTCCCGGAGTTCACCGGCCGCCTGTGCCCCGCACCCTGCGAAGGCGCATGTGTGCT
GGGTATCGGCGATGACCCGGTGAACATCAAGACCGTTGAGTACACCATCGTTGAGCACGCTTGGAAGGAAGGTTGGATCA
AACCGATCAAGCCGAGCTTCAAGACGGGCCAGCGCGTGGCCGTCGTCGGTTCCGGTCCGGCCGGCCTGGCTGCTGCGCAG
CAGCTGACACGCGCGGGCCACGACGTCACCGTTTTCGAGCGCGCCGACCGCATCGGCGGATTGATGCGCTACGGCGTGCC
CGAGTACAAGATGGAAAAGAAGTGGATCGACCGCCGTCTGGAGCAGATGGAGGCCGAGGGCACGAAGTTCGTCACTAACA
CCTCGCCTACCGGTGAGCAGCTGAAGGAGTTCGACGGCGTGATCGTCGCTATTGGCTCCACCGTCGGCCGCGACCTGCCC
GTGGTGGGCCGCGACCTGAAGGGCGTTTACCAGGCGATGGAGTACCTGCCGGAGGCCAACAAGGTCTGTGAGGGCGACCG
CGAGGTATCCACCATCAACGCCAAGGGCAAGCGCGTGGTGATCATCGGCGGTGGCGATACCGGTACCGACTGCTTCGGCA
CCGCACTGCGCCAGGGCGCCATCTCGGTGTCTCAGTTCGACATCACCCCGAAGCCGCCGAAGACTCGAGGTGCCTCCACC
CCGTGGCCGACTTACCCGCGCATTTGGCGCGTGGCGTCCGCCCACGAGGAGGGCGAGTACCGCGTGACCGGCAACGAGTC
CGCCGACGAGATCGCCGCCCTGGGCCTGGCCGAGCGCCAGCCGGGCGACGAGCTGGGCATCCGCCGTTACTCCATCAACA
CGGTGGAGATCACCGGCGACGGCGAGAAGGTCACTGGCCTGAAGGGCGCCGAGTGCCGCTTCGGCGAGAACGGCCTGGAG
AACATCCCGGAGTCGGACTTCGAGATGGACGCCGACCTGGTGCTGCTGGCGATGGGCTTTGTCTCCGTGGAGGAGACTCC
GGTGGTGCGGGACTTGGGCCTGAAGATTGGCGAGCGCGGCCGCCTGGAGCGCGATGACACCTTCCGCGCCCAGCCCACCA
ACGCTGAGTTCGCGGACATTCCGGTGTTCGTCGCCGGCGACGCGGGCCGTGGCCAGTCCCTGATCGTGTGGGGCATCTCG
GAGGGCCGTTCTGCAGCAGCGGAGCTGGACCGCGAGCTGATGGGCGAGACCGCCCTGCCACGCCCGATCAATCCGACTGA
CGTGGCGATGCAGGCTTGA

Upstream 100 bases:

>100_bases
CCGCGACTACGCGCGCGTGCTCAAGACGATCGCACGAGCCGAAGCCGAAGGCTTGGACAACGACGGCATCGCCGCCGCAA
TCATGGAGGAAGTGAAGTAA

Downstream 100 bases:

>100_bases
GCCTAGCCGCTTACCTTGGGCTGCTCGGTGCCTGGGTGCTGGCCATTACGGTGCCAGGTCCAGACACCGTGCAGCTCATT
CGTTTGGGTGCCCGTTCGCG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MADPRGFLKHRRESAAHRPVPLRLLDWNEVYEDFSDDKVQTQASRCMDCGIPFCHDGCPLGNIIPEWNDLVRKGNWREAY
DRLHATNNFPEFTGRLCPAPCEGACVLGIGDDPVNIKTVEYTIVEHAWKEGWIKPIKPSFKTGQRVAVVGSGPAGLAAAQ
QLTRAGHDVTVFERADRIGGLMRYGVPEYKMEKKWIDRRLEQMEAEGTKFVTNTSPTGEQLKEFDGVIVAIGSTVGRDLP
VVGRDLKGVYQAMEYLPEANKVCEGDREVSTINAKGKRVVIIGGGDTGTDCFGTALRQGAISVSQFDITPKPPKTRGAST
PWPTYPRIWRVASAHEEGEYRVTGNESADEIAALGLAERQPGDELGIRRYSINTVEITGDGEKVTGLKGAECRFGENGLE
NIPESDFEMDADLVLLAMGFVSVEETPVVRDLGLKIGERGRLERDDTFRAQPTNAEFADIPVFVAGDAGRGQSLIVWGIS
EGRSAAAELDRELMGETALPRPINPTDVAMQA

Sequences:

>Translated_512_residues
MADPRGFLKHRRESAAHRPVPLRLLDWNEVYEDFSDDKVQTQASRCMDCGIPFCHDGCPLGNIIPEWNDLVRKGNWREAY
DRLHATNNFPEFTGRLCPAPCEGACVLGIGDDPVNIKTVEYTIVEHAWKEGWIKPIKPSFKTGQRVAVVGSGPAGLAAAQ
QLTRAGHDVTVFERADRIGGLMRYGVPEYKMEKKWIDRRLEQMEAEGTKFVTNTSPTGEQLKEFDGVIVAIGSTVGRDLP
VVGRDLKGVYQAMEYLPEANKVCEGDREVSTINAKGKRVVIIGGGDTGTDCFGTALRQGAISVSQFDITPKPPKTRGAST
PWPTYPRIWRVASAHEEGEYRVTGNESADEIAALGLAERQPGDELGIRRYSINTVEITGDGEKVTGLKGAECRFGENGLE
NIPESDFEMDADLVLLAMGFVSVEETPVVRDLGLKIGERGRLERDDTFRAQPTNAEFADIPVFVAGDAGRGQSLIVWGIS
EGRSAAAELDRELMGETALPRPINPTDVAMQA
>Mature_511_residues
ADPRGFLKHRRESAAHRPVPLRLLDWNEVYEDFSDDKVQTQASRCMDCGIPFCHDGCPLGNIIPEWNDLVRKGNWREAYD
RLHATNNFPEFTGRLCPAPCEGACVLGIGDDPVNIKTVEYTIVEHAWKEGWIKPIKPSFKTGQRVAVVGSGPAGLAAAQQ
LTRAGHDVTVFERADRIGGLMRYGVPEYKMEKKWIDRRLEQMEAEGTKFVTNTSPTGEQLKEFDGVIVAIGSTVGRDLPV
VGRDLKGVYQAMEYLPEANKVCEGDREVSTINAKGKRVVIIGGGDTGTDCFGTALRQGAISVSQFDITPKPPKTRGASTP
WPTYPRIWRVASAHEEGEYRVTGNESADEIAALGLAERQPGDELGIRRYSINTVEITGDGEKVTGLKGAECRFGENGLEN
IPESDFEMDADLVLLAMGFVSVEETPVVRDLGLKIGERGRLERDDTFRAQPTNAEFADIPVFVAGDAGRGQSLIVWGISE
GRSAAAELDRELMGETALPRPINPTDVAMQA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=291, Percent_Identity=25.7731958762887, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789606, Length=501, Percent_Identity=32.7345309381238, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1788811, Length=482, Percent_Identity=34.4398340248963, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082180, Length=487, Percent_Identity=29.3634496919918, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789243, Length=458, Percent_Identity=29.0393013100437, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788468, Length=483, Percent_Identity=23.8095238095238, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=520, Percent_Identity=45.3846153846154, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984108, Length=312, Percent_Identity=25.6410256410256, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=512, Percent_Identity=44.53125, Blast_Score=406, Evalue=1e-114,
Organism=Drosophila melanogaster, GI28574881, Length=515, Percent_Identity=45.4368932038835, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24665539, Length=515, Percent_Identity=45.4368932038835, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24665547, Length=515, Percent_Identity=45.6310679611651, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24665543, Length=515, Percent_Identity=45.6310679611651, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24640763, Length=321, Percent_Identity=27.1028037383178, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI18858217, Length=321, Percent_Identity=27.1028037383178, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 56118; Mature: 55987

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPRGFLKHRRESAAHRPVPLRLLDWNEVYEDFSDDKVQTQASRCMDCGIPFCHDGCPL
CCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GNIIPEWNDLVRKGNWREAYDRLHATNNFPEFTGRLCPAPCEGACVLGIGDDPVNIKTVE
CCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
YTIVEHAWKEGWIKPIKPSFKTGQRVAVVGSGPAGLAAAQQLTRAGHDVTVFERADRIGG
EHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHH
LMRYGVPEYKMEKKWIDRRLEQMEAEGTKFVTNTSPTGEQLKEFDGVIVAIGSTVGRDLP
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCEEEEECCCCCCCCC
VVGRDLKGVYQAMEYLPEANKVCEGDREVSTINAKGKRVVIIGGGDTGTDCFGTALRQGA
CCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCC
ISVSQFDITPKPPKTRGASTPWPTYPRIWRVASAHEEGEYRVTGNESADEIAALGLAERQ
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCC
PGDELGIRRYSINTVEITGDGEKVTGLKGAECRFGENGLENIPESDFEMDADLVLLAMGF
CCHHHCEEEEEEEEEEEECCCCEECCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHH
VSVEETPVVRDLGLKIGERGRLERDDTFRAQPTNAEFADIPVFVAGDAGRGQSLIVWGIS
HCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECC
EGRSAAAELDRELMGETALPRPINPTDVAMQA
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADPRGFLKHRRESAAHRPVPLRLLDWNEVYEDFSDDKVQTQASRCMDCGIPFCHDGCPL
CCCHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GNIIPEWNDLVRKGNWREAYDRLHATNNFPEFTGRLCPAPCEGACVLGIGDDPVNIKTVE
CCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
YTIVEHAWKEGWIKPIKPSFKTGQRVAVVGSGPAGLAAAQQLTRAGHDVTVFERADRIGG
EHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHH
LMRYGVPEYKMEKKWIDRRLEQMEAEGTKFVTNTSPTGEQLKEFDGVIVAIGSTVGRDLP
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCEEEEECCCCCCCCC
VVGRDLKGVYQAMEYLPEANKVCEGDREVSTINAKGKRVVIIGGGDTGTDCFGTALRQGA
CCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCC
ISVSQFDITPKPPKTRGASTPWPTYPRIWRVASAHEEGEYRVTGNESADEIAALGLAERQ
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCC
PGDELGIRRYSINTVEITGDGEKVTGLKGAECRFGENGLENIPESDFEMDADLVLLAMGF
CCHHHCEEEEEEEEEEEECCCCEECCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHH
VSVEETPVVRDLGLKIGERGRLERDDTFRAQPTNAEFADIPVFVAGDAGRGQSLIVWGIS
HCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECC
EGRSAAAELDRELMGETALPRPINPTDVAMQA
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]