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The map label for this gene is recF

Identifier: 68535065

GI number: 68535065

Start: 3596

End: 4873

Strand: Direct

Name: recF

Synonym: jk0003

Alternate gene names: 68535065

Gene position: 3596-4873 (Clockwise)

Preceding gene: 68535064

Following gene: 68535066

Centisome position: 0.15

GC content: 59.39

Gene sequence:

>1278_bases
GTGTATGTCTCCAATCTTCGTCTGAGTAATTACAGGTCGTGGGAGGAACTCGACCTGCAGCTCAGCCCTGGCATCACGAT
CTTTTCCGGCCCTAACGGCCACGGGAAAACCAATATCGTTGAGGCACTCGGTTACCTTGCCCACTTAAGCTCGCACCGTG
TTAACTCTGATGCCGCCCTGGTGCGACGTGGAGAAGAGATTGCCAACATCTCTGCGACCGCCGTGAACAATGGCCGCGAA
CTCACTGCTCATCTGGCTATCCGTGCTCGGGGCTCGAACCGCGCGCACATCAACCGGGCTGCTATGAATTCCCAACGGGA
TCTGCTCGGAGTCGTGCGAACGACGTTGTTTTCTCCCGAAGATTTGGCGCTGATCCGCGGCGAGCCTGAGCAGCGCCGTC
ATTTTCTAGATGCGATCATGGTCGCCCGATACCCACGCCTAGCTGCGGTAAAGGCGGATTACGACAAAGCCTTGCGCCAG
CGAAATGCGCTGCTACGGCAGTCGGCCTTTGCTTTGCGGCTCGTCGTCGGCGCTCCGAAGGGAGCAAGCCACAACCTTTC
GGAAGACATCAAGGCCGATGCTGAATCCGCCCTGGCTACTCTCGATGTGTGGGATAGTCAGCTGGCCGCGCTGGGCGCAC
AGATCATGTCGGCGCGCGTTCAAATCGTTCACGACCTCGCCCCGCATCTTCAGCAGACGTATCAATCTCTCGCTCCGCAA
TCGCGCCCCGCGCACATGTCCTACACGTCCACCATCGATGTAGAGCTTGCTGATCTGGGTATTCGCCTTGGTGTGAGCGA
GCCGAACCAGCCCACGGCTTTACTCAGCCCTGAGATCGCAGAGGCTACGTTGCTGCAGGCTTTCGCCAACAAGCGCCCCC
AGGAGGTCGAGCGCGGGACCACGCTGCTTGGCCCTCACCGCGATGATGTGAATTTGATCCTCGGCCACCAGCCCGCCAAG
GGCTACGCCTCGCACGGTGAATCCTGGTCCTTCGCTCTCTCGCTGCGGCTTGCTGCGTTTTTCATGCAGCGCGGGGACGG
GGTTGAGCCCGTCGTGATCCTCGACGATGTTTTTGCAGAGCTCGATTCTTCTCGACGCCAGCACCTCGTCGACCTCATCT
CCAGCGCCGAACAGGTCCTCATCACGGCCGCGGTGGATGAGGACATTCCAGAGGCTCTGCGGGATGTGGCGAAGATCTAC
ACTATTGATGAACTCAAGAATTCCGCGTTAAGCACGGCGGAACGCGCTGTAAAGGATGGCGAGGCTGATGGCGACTGA

Upstream 100 bases:

>100_bases
CGCAACTCCGGAAACGGACTTCACCTACCTGCTGATGCCCGTGCGCCTGCCGGGCTAAGCCAAGGTTGATTTGGTTTTCT
AGGTTGATTTCAAGCTAACC

Downstream 100 bases:

>100_bases
CCCAGTAGCCCAAGCCCTCGCTGCCATGAAGAAGGCCGGTGGTTCCCCGCAGGCTGAGTTGCCGCTGGTGGCGTCACCAA
AAAAGAACAAGAAGACAACG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MYVSNLRLSNYRSWEELDLQLSPGITIFSGPNGHGKTNIVEALGYLAHLSSHRVNSDAALVRRGEEIANISATAVNNGRE
LTAHLAIRARGSNRAHINRAAMNSQRDLLGVVRTTLFSPEDLALIRGEPEQRRHFLDAIMVARYPRLAAVKADYDKALRQ
RNALLRQSAFALRLVVGAPKGASHNLSEDIKADAESALATLDVWDSQLAALGAQIMSARVQIVHDLAPHLQQTYQSLAPQ
SRPAHMSYTSTIDVELADLGIRLGVSEPNQPTALLSPEIAEATLLQAFANKRPQEVERGTTLLGPHRDDVNLILGHQPAK
GYASHGESWSFALSLRLAAFFMQRGDGVEPVVILDDVFAELDSSRRQHLVDLISSAEQVLITAAVDEDIPEALRDVAKIY
TIDELKNSALSTAERAVKDGEADGD

Sequences:

>Translated_425_residues
MYVSNLRLSNYRSWEELDLQLSPGITIFSGPNGHGKTNIVEALGYLAHLSSHRVNSDAALVRRGEEIANISATAVNNGRE
LTAHLAIRARGSNRAHINRAAMNSQRDLLGVVRTTLFSPEDLALIRGEPEQRRHFLDAIMVARYPRLAAVKADYDKALRQ
RNALLRQSAFALRLVVGAPKGASHNLSEDIKADAESALATLDVWDSQLAALGAQIMSARVQIVHDLAPHLQQTYQSLAPQ
SRPAHMSYTSTIDVELADLGIRLGVSEPNQPTALLSPEIAEATLLQAFANKRPQEVERGTTLLGPHRDDVNLILGHQPAK
GYASHGESWSFALSLRLAAFFMQRGDGVEPVVILDDVFAELDSSRRQHLVDLISSAEQVLITAAVDEDIPEALRDVAKIY
TIDELKNSALSTAERAVKDGEADGD
>Mature_425_residues
MYVSNLRLSNYRSWEELDLQLSPGITIFSGPNGHGKTNIVEALGYLAHLSSHRVNSDAALVRRGEEIANISATAVNNGRE
LTAHLAIRARGSNRAHINRAAMNSQRDLLGVVRTTLFSPEDLALIRGEPEQRRHFLDAIMVARYPRLAAVKADYDKALRQ
RNALLRQSAFALRLVVGAPKGASHNLSEDIKADAESALATLDVWDSQLAALGAQIMSARVQIVHDLAPHLQQTYQSLAPQ
SRPAHMSYTSTIDVELADLGIRLGVSEPNQPTALLSPEIAEATLLQAFANKRPQEVERGTTLLGPHRDDVNLILGHQPAK
GYASHGESWSFALSLRLAAFFMQRGDGVEPVVILDDVFAELDSSRRQHLVDLISSAEQVLITAAVDEDIPEALRDVAKIY
TIDELKNSALSTAERAVKDGEADGD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=410, Percent_Identity=23.9024390243902, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CORJK (Q4JYF5)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_249770.1
- ProteinModelPortal:   Q4JYF5
- SMR:   Q4JYF5
- STRING:   Q4JYF5
- GeneID:   3431931
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0003
- NMPDR:   fig|306537.3.peg.1576
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   CJEI306537:JK0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 46382; Mature: 46382

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVSNLRLSNYRSWEELDLQLSPGITIFSGPNGHGKTNIVEALGYLAHLSSHRVNSDAAL
CCCCCCCCCCCCCHHHCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
VRRGEEIANISATAVNNGRELTAHLAIRARGSNRAHINRAAMNSQRDLLGVVRTTLFSPE
HHCCHHHHCCEEEEECCCCEEEEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHCCCC
DLALIRGEPEQRRHFLDAIMVARYPRLAAVKADYDKALRQRNALLRQSAFALRLVVGAPK
CEEEECCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
GASHNLSEDIKADAESALATLDVWDSQLAALGAQIMSARVQIVHDLAPHLQQTYQSLAPQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SRPAHMSYTSTIDVELADLGIRLGVSEPNQPTALLSPEIAEATLLQAFANKRPQEVERGT
CCCCCEEECCEEEEEEECCCEEECCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHHCCC
TLLGPHRDDVNLILGHQPAKGYASHGESWSFALSLRLAAFFMQRGDGVEPVVILDDVFAE
EECCCCCCCCEEEEECCCCCCHHCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
LDSSRRQHLVDLISSAEQVLITAAVDEDIPEALRDVAKIYTIDELKNSALSTAERAVKDG
HHHHHHHHHHHHHHCHHHEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EADGD
CCCCC
>Mature Secondary Structure
MYVSNLRLSNYRSWEELDLQLSPGITIFSGPNGHGKTNIVEALGYLAHLSSHRVNSDAAL
CCCCCCCCCCCCCHHHCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
VRRGEEIANISATAVNNGRELTAHLAIRARGSNRAHINRAAMNSQRDLLGVVRTTLFSPE
HHCCHHHHCCEEEEECCCCEEEEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHCCCC
DLALIRGEPEQRRHFLDAIMVARYPRLAAVKADYDKALRQRNALLRQSAFALRLVVGAPK
CEEEECCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
GASHNLSEDIKADAESALATLDVWDSQLAALGAQIMSARVQIVHDLAPHLQQTYQSLAPQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SRPAHMSYTSTIDVELADLGIRLGVSEPNQPTALLSPEIAEATLLQAFANKRPQEVERGT
CCCCCEEECCEEEEEEECCCEEECCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHHCCC
TLLGPHRDDVNLILGHQPAKGYASHGESWSFALSLRLAAFFMQRGDGVEPVVILDDVFAE
EECCCCCCCCEEEEECCCCCCHHCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
LDSSRRQHLVDLISSAEQVLITAAVDEDIPEALRDVAKIYTIDELKNSALSTAERAVKDG
HHHHHHHHHHHHHHCHHHEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EADGD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA