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The map label for this gene is dnaN [H]

Identifier: 68535064

GI number: 68535064

Start: 2369

End: 3553

Strand: Direct

Name: dnaN [H]

Synonym: jk0002

Alternate gene names: 68535064

Gene position: 2369-3553 (Clockwise)

Preceding gene: 68535063

Following gene: 68535065

Centisome position: 0.1

GC content: 60.59

Gene sequence:

>1185_bases
ATGGATAGCCAAGCAGTGAGTTTCATTGTTCCCAAGGATGACTTCGCATCCGCCCTCGGTTGGGTAGCTCGCTCCCTGCC
TAGCAAGCCGACCCAACCCATTTTGCGCGGTGTGATGATTGTCGCCGATGACGAAGGGTTGGAACTTTCAGGATTTGACC
GCGAAGTCTCCACTCGCGTGCGCATCAATGCCGAGGTTAACGAGCCCGGCAAGATTCTCGTAGCTGGCAAGCTAGCTTCC
GACATCGTCGGCTCCTTGCCGAATAAACCGATCAACATCGAGTTCGACGGCACCACTGTGATTCTCAAGACTGGTAAGTC
CAAGTTCGAGCTACCGGCAATGACCATCGAGGACTACCCGGTTCTGCCGGAACTGCCGGAAGTCACCGGCACTATTGACC
CGAACCTCTTCGCGGAATCCATTTCGCAGGTCGCCGTAGCGGCTGGACGCGACGACACCCTACCGATGCTCACTGGCGTG
CACGTTGAAATCGATGGCGAGAATGTCATCATGGTTGCCACCGACCGCTTCCGTCTGGCCGTCCGCAGTTTCCAGTGGAA
CCCGGCACAGGCCGATGCGCAGGCGAAGCTGCTGATTCCAGCCCGCACCCTGGCGGATACCGCGCGCTCCATGGATCCGA
ATAACACCGATCCCATCGAAATCGCAGTGGGTAGCGGCGAGGACATCGGCGCCGATGGCCTGCTGGGTATTTCCACCGAG
TCGCGCCACACGACCACGCGCTTGCTCGACGCGGAATTCCCAAAGTTCCGCCCGCTTCTGCCGAAGACGCACACCGCCCT
AGCCAGCGTGGAGATTGCTCCGCTGCAGGAGGCAATCAAGCGCGTCTCTATCGTCGCCGAGCGGAACTCCCAGATCCGGA
TGGACTTCAAGGACAACACCGTCACCCTTTCCGCGGGTGGCAGCGACGTCGGATCCGCTACGGAAACTCTGGACTGCGCT
TTCGCCGGCGAGCCGCTGGTCATCGCGTTTAACCCGAGCTACCTAAAGGAAGGCCTGGCGGCCATCCACACGCCGCGTGT
TGTTTTCGGCTTCACCATGGCCTCCCGTCCCGCTATCTTGCTGCCGGATCCGGGCGAACTGCCGGAGGCCGATGCAGAGG
GCAACTTCGCAACTCCGGAAACGGACTTCACCTACCTGCTGATGCCCGTGCGCCTGCCGGGCTAA

Upstream 100 bases:

>100_bases
TTAACCTTGATCAGACGAACCTCGAGGACCGCTAGACCTCGAGAACCCCGAAAACCTCAAAAACTACGAGAGCGACGAGA
GCACGGAAGTTAGGTTGAGC

Downstream 100 bases:

>100_bases
GCCAAGGTTGATTTGGTTTTCTAGGTTGATTTCAAGCTAACCGTGTATGTCTCCAATCTTCGTCTGAGTAATTACAGGTC
GTGGGAGGAACTCGACCTGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MDSQAVSFIVPKDDFASALGWVARSLPSKPTQPILRGVMIVADDEGLELSGFDREVSTRVRINAEVNEPGKILVAGKLAS
DIVGSLPNKPINIEFDGTTVILKTGKSKFELPAMTIEDYPVLPELPEVTGTIDPNLFAESISQVAVAAGRDDTLPMLTGV
HVEIDGENVIMVATDRFRLAVRSFQWNPAQADAQAKLLIPARTLADTARSMDPNNTDPIEIAVGSGEDIGADGLLGISTE
SRHTTTRLLDAEFPKFRPLLPKTHTALASVEIAPLQEAIKRVSIVAERNSQIRMDFKDNTVTLSAGGSDVGSATETLDCA
FAGEPLVIAFNPSYLKEGLAAIHTPRVVFGFTMASRPAILLPDPGELPEADAEGNFATPETDFTYLLMPVRLPG

Sequences:

>Translated_394_residues
MDSQAVSFIVPKDDFASALGWVARSLPSKPTQPILRGVMIVADDEGLELSGFDREVSTRVRINAEVNEPGKILVAGKLAS
DIVGSLPNKPINIEFDGTTVILKTGKSKFELPAMTIEDYPVLPELPEVTGTIDPNLFAESISQVAVAAGRDDTLPMLTGV
HVEIDGENVIMVATDRFRLAVRSFQWNPAQADAQAKLLIPARTLADTARSMDPNNTDPIEIAVGSGEDIGADGLLGISTE
SRHTTTRLLDAEFPKFRPLLPKTHTALASVEIAPLQEAIKRVSIVAERNSQIRMDFKDNTVTLSAGGSDVGSATETLDCA
FAGEPLVIAFNPSYLKEGLAAIHTPRVVFGFTMASRPAILLPDPGELPEADAEGNFATPETDFTYLLMPVRLPG
>Mature_394_residues
MDSQAVSFIVPKDDFASALGWVARSLPSKPTQPILRGVMIVADDEGLELSGFDREVSTRVRINAEVNEPGKILVAGKLAS
DIVGSLPNKPINIEFDGTTVILKTGKSKFELPAMTIEDYPVLPELPEVTGTIDPNLFAESISQVAVAAGRDDTLPMLTGV
HVEIDGENVIMVATDRFRLAVRSFQWNPAQADAQAKLLIPARTLADTARSMDPNNTDPIEIAVGSGEDIGADGLLGISTE
SRHTTTRLLDAEFPKFRPLLPKTHTALASVEIAPLQEAIKRVSIVAERNSQIRMDFKDNTVTLSAGGSDVGSATETLDCA
FAGEPLVIAFNPSYLKEGLAAIHTPRVVFGFTMASRPAILLPDPGELPEADAEGNFATPETDFTYLLMPVRLPG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=351, Percent_Identity=26.2108262108262, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42211; Mature: 42211

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSQAVSFIVPKDDFASALGWVARSLPSKPTQPILRGVMIVADDEGLELSGFDREVSTRV
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCEECCCCCCCEEEE
RINAEVNEPGKILVAGKLASDIVGSLPNKPINIEFDGTTVILKTGKSKFELPAMTIEDYP
EEEEEECCCCEEEEECCHHHHHHHHCCCCCEEEEECCEEEEEECCCCCEECCEEEECCCC
VLPELPEVTGTIDPNLFAESISQVAVAAGRDDTLPMLTGVHVEIDGENVIMVATDRFRLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECCCEEEE
VRSFQWNPAQADAQAKLLIPARTLADTARSMDPNNTDPIEIAVGSGEDIGADGLLGISTE
EECCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECCC
SRHTTTRLLDAEFPKFRPLLPKTHTALASVEIAPLQEAIKRVSIVAERNSQIRMDFKDNT
CCCCCEEEECCCCCCCCCCCCCCHHHHEEEEEHHHHHHHHHHEEEEECCCEEEEEECCCE
VTLSAGGSDVGSATETLDCAFAGEPLVIAFNPSYLKEGLAAIHTPRVVFGFTMASRPAIL
EEEECCCCCCCCCHHEEEEEECCCCEEEEECHHHHHHHHHHHCCCEEEEEEEECCCCEEE
LPDPGELPEADAEGNFATPETDFTYLLMPVRLPG
ECCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MDSQAVSFIVPKDDFASALGWVARSLPSKPTQPILRGVMIVADDEGLELSGFDREVSTRV
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCEECCCCCCCEEEE
RINAEVNEPGKILVAGKLASDIVGSLPNKPINIEFDGTTVILKTGKSKFELPAMTIEDYP
EEEEEECCCCEEEEECCHHHHHHHHCCCCCEEEEECCEEEEEECCCCCEECCEEEECCCC
VLPELPEVTGTIDPNLFAESISQVAVAAGRDDTLPMLTGVHVEIDGENVIMVATDRFRLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECCCEEEE
VRSFQWNPAQADAQAKLLIPARTLADTARSMDPNNTDPIEIAVGSGEDIGADGLLGISTE
EECCCCCCCCCCCEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECCC
SRHTTTRLLDAEFPKFRPLLPKTHTALASVEIAPLQEAIKRVSIVAERNSQIRMDFKDNT
CCCCCEEEECCCCCCCCCCCCCCHHHHEEEEEHHHHHHHHHHEEEEECCCEEEEEECCCE
VTLSAGGSDVGSATETLDCAFAGEPLVIAFNPSYLKEGLAAIHTPRVVFGFTMASRPAIL
EEEECCCCCCCCCHHEEEEEECCCCEEEEECHHHHHHHHHHHCCCEEEEEEEECCCCEEE
LPDPGELPEADAEGNFATPETDFTYLLMPVRLPG
ECCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8733228 [H]