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The map label for this gene is pme [H]

Identifier: 66768171

GI number: 66768171

Start: 2245568

End: 2246773

Strand: Reverse

Name: pme [H]

Synonym: XC_1850

Alternate gene names: 66768171

Gene position: 2246773-2245568 (Counterclockwise)

Preceding gene: 66768175

Following gene: 77761171

Centisome position: 43.64

GC content: 65.09

Gene sequence:

>1206_bases
ATGTCTGCATCGCATATCCGCACCACCTGCCTCGCCACCGCGCTGCTGAGCCTGATCAGCCTTGATGCTGCTGCCCTTAC
CGGCACCGCCAGCCGCCCGCAGCTCACCAGCAGCGAAGCAGGCACGTATACGATCAGCAAGGCGCTCGCCAAGGCCGGCC
CGATCACAGCGTTGGTGACCGACAACTGGAACCCCACCGCCGGCGTGGCGCTGCTGACCGCCGACTTTGCAGTTGCCGCC
GACGGCTCCACCCGTTATCGCACGGTGCAATCGGCAATCGACGCGGCGGTGGCCGCCGGTGGCAGCACGCGCCGCTACAT
CAGCGTCAAGGCCGGCACCTACACCGAGCTGGTGTGCGTGCCGAGCGGTGCACCGCCACTGACCCTGTTCGGGCTTGGCA
GCGCCACGACCGACACCGTCATCCGCTACAACAATGCCAACCCCACGCCCAAGCCAACCGGCACCGCAAGTCACCCCTGC
GCCAGCAATGCGTCAGCCGCCACAGTGGGCACTTCCAATAGCGCGACGGTTACCGTACGTGCCAGCGGCTTCCAGGCGCG
GCATCTGCGCTTCGACAATAACTATGTCGAAGGCACTTACGCCGACAACAATCAATCGGCAGTGGCACTGGCCGTGCGCG
GCGACCAGGCCAGCTTCGAGGACGTGCTGGTCACCGGCAACCAGGACACCTTGCTGATCAGCGCGACCAACGCCGCCAAC
GTGATCCGCGCGTATTTCAAGAGCAGCACGATCGAAGGCGATGTCGATTTCATCTTCGGGTCCGGCATCGGCGTGTTCGA
CAACGCAATCATTCGCTCGGCCGGCGCGCGCCTGGGCAGCAGCCGTGGTGGCTACATCTTCGCGCCAAGCACGCGCCCGG
GCAGCGGCTACGGCTTTCTGGCGATCAACAGCCGCTTCGTAGCGGGCTCCGGTTCGCCGGACAATCAAACCTACCTCGGG
CGCGCATGGGACGAAGGCGTTAGTGGCCTGTCCGCGTACGTCAACGGCACCTCGCCCAATGGCCAGGTGACCATCCGCGA
TTCCACGCTGGGCAGCCACATCCGCAAGACCGCGCCATGGAATGCCTCCACCGCCAGCCGCCCGTTCTGCAGCAGCAACT
GCACCAACTCGGCCAACCGCTTTTTTTCGAATACGGCAACAGCGGTGCTGGCAGTGCCGACTGATCCAGGCCGCAGCGTT
CGATAG

Upstream 100 bases:

>100_bases
GGTGTGCCTCGCCTGCGTCTGCGTTGCCAGCACGCGGCGCACCGCTGGTGTTTGGCGTTTGCCATCCGGCAAGCGCATCC
TCTCTGGAGAGCGTCATCCC

Downstream 100 bases:

>100_bases
CAGCGTCGGCCGCCGGTACGCGTGTTTGCGCGTGCCGGCGGCGGCGCCAGCGCAGTGGATCTGCTTCGCTAACAGGTGAT
CTGCCTCGCTGTGATTACGC

Product: putative pectinesterase

Products: NA

Alternate protein names: PE; Pectin methylesterase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSASHIRTTCLATALLSLISLDAAALTGTASRPQLTSSEAGTYTISKALAKAGPITALVTDNWNPTAGVALLTADFAVAA
DGSTRYRTVQSAIDAAVAAGGSTRRYISVKAGTYTELVCVPSGAPPLTLFGLGSATTDTVIRYNNANPTPKPTGTASHPC
ASNASAATVGTSNSATVTVRASGFQARHLRFDNNYVEGTYADNNQSAVALAVRGDQASFEDVLVTGNQDTLLISATNAAN
VIRAYFKSSTIEGDVDFIFGSGIGVFDNAIIRSAGARLGSSRGGYIFAPSTRPGSGYGFLAINSRFVAGSGSPDNQTYLG
RAWDEGVSGLSAYVNGTSPNGQVTIRDSTLGSHIRKTAPWNASTASRPFCSSNCTNSANRFFSNTATAVLAVPTDPGRSV
R

Sequences:

>Translated_401_residues
MSASHIRTTCLATALLSLISLDAAALTGTASRPQLTSSEAGTYTISKALAKAGPITALVTDNWNPTAGVALLTADFAVAA
DGSTRYRTVQSAIDAAVAAGGSTRRYISVKAGTYTELVCVPSGAPPLTLFGLGSATTDTVIRYNNANPTPKPTGTASHPC
ASNASAATVGTSNSATVTVRASGFQARHLRFDNNYVEGTYADNNQSAVALAVRGDQASFEDVLVTGNQDTLLISATNAAN
VIRAYFKSSTIEGDVDFIFGSGIGVFDNAIIRSAGARLGSSRGGYIFAPSTRPGSGYGFLAINSRFVAGSGSPDNQTYLG
RAWDEGVSGLSAYVNGTSPNGQVTIRDSTLGSHIRKTAPWNASTASRPFCSSNCTNSANRFFSNTATAVLAVPTDPGRSV
R
>Mature_400_residues
SASHIRTTCLATALLSLISLDAAALTGTASRPQLTSSEAGTYTISKALAKAGPITALVTDNWNPTAGVALLTADFAVAAD
GSTRYRTVQSAIDAAVAAGGSTRRYISVKAGTYTELVCVPSGAPPLTLFGLGSATTDTVIRYNNANPTPKPTGTASHPCA
SNASAATVGTSNSATVTVRASGFQARHLRFDNNYVEGTYADNNQSAVALAVRGDQASFEDVLVTGNQDTLLISATNAANV
IRAYFKSSTIEGDVDFIFGSGIGVFDNAIIRSAGARLGSSRGGYIFAPSTRPGSGYGFLAINSRFVAGSGSPDNQTYLGR
AWDEGVSGLSAYVNGTSPNGQVTIRDSTLGSHIRKTAPWNASTASRPFCSSNCTNSANRFFSNTATAVLAVPTDPGRSVR

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

Organism=Escherichia coli, GI1786989, Length=368, Percent_Identity=32.3369565217391, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 41228; Mature: 41097

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00503 PECTINESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASHIRTTCLATALLSLISLDAAALTGTASRPQLTSSEAGTYTISKALAKAGPITALVT
CCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
DNWNPTAGVALLTADFAVAADGSTRYRTVQSAIDAAVAAGGSTRRYISVKAGTYTELVCV
CCCCCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEE
PSGAPPLTLFGLGSATTDTVIRYNNANPTPKPTGTASHPCASNASAATVGTSNSATVTVR
CCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEE
ASGFQARHLRFDNNYVEGTYADNNQSAVALAVRGDQASFEDVLVTGNQDTLLISATNAAN
ECCCEEEEEEECCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCHHH
VIRAYFKSSTIEGDVDFIFGSGIGVFDNAIIRSAGARLGSSRGGYIFAPSTRPGSGYGFL
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEE
AINSRFVAGSGSPDNQTYLGRAWDEGVSGLSAYVNGTSPNGQVTIRDSTLGSHIRKTAPW
EEEEEEEECCCCCCCCEEECCCHHHHCCCEEEEEECCCCCCEEEEEECCCCHHHHHCCCC
NASTASRPFCSSNCTNSANRFFSNTATAVLAVPTDPGRSVR
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
SASHIRTTCLATALLSLISLDAAALTGTASRPQLTSSEAGTYTISKALAKAGPITALVT
CCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
DNWNPTAGVALLTADFAVAADGSTRYRTVQSAIDAAVAAGGSTRRYISVKAGTYTELVCV
CCCCCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEE
PSGAPPLTLFGLGSATTDTVIRYNNANPTPKPTGTASHPCASNASAATVGTSNSATVTVR
CCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEE
ASGFQARHLRFDNNYVEGTYADNNQSAVALAVRGDQASFEDVLVTGNQDTLLISATNAAN
ECCCEEEEEEECCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCHHH
VIRAYFKSSTIEGDVDFIFGSGIGVFDNAIIRSAGARLGSSRGGYIFAPSTRPGSGYGFL
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEE
AINSRFVAGSGSPDNQTYLGRAWDEGVSGLSAYVNGTSPNGQVTIRDSTLGSHIRKTAPW
EEEEEEEECCCCCCCCEEECCCHHHHCCCEEEEEECCCCCCEEEEEECCCCHHHHHCCCC
NASTASRPFCSSNCTNSANRFFSNTATAVLAVPTDPGRSVR
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]