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The map label for this gene is potF [H]

Identifier: 66768093

GI number: 66768093

Start: 2155381

End: 2156496

Strand: Direct

Name: potF [H]

Synonym: XC_1772

Alternate gene names: 66768093

Gene position: 2155381-2156496 (Clockwise)

Preceding gene: 66768092

Following gene: 66768094

Centisome position: 41.86

GC content: 62.46

Gene sequence:

>1116_bases
ATGACGCTGCGACTGCTCGCCCTGACCCTGTCCATCACCCTGTTGTCCGGCTGTGGCGGCGGTGGCGCCCCGGGCAAGGC
CGATGCACAGGCCAAGGTGCTCAACGTCTACAACTACTCCGACTACATCGCCGAAGACACCATCCCCGCGTTCGAAAAGA
GCACCGGCATCAAGGTCACCTACGACGTGTTCGACAGCGACGAAATGGTGGAAACCAAACTGCTCGCCGGTGGCAGCGAC
TACGACGTGGTGGTGCCCACGCTCAACTTCTTTGGCCGCCAGATCCAGGCCGGCGTGTTCCTGCCGCTGGATAAAAGCAA
GATTCCGAACCTGGCCAATCTCGACCCGGACATCATGCGCCGCATCGCCGCGCAGGACCCCGGCAACACCTATGGCGTAC
CGTACATGATCGGTACCACCGGCATCGGCTACAACGTCGACAAGCTCAAGGCGGCGTTTGGCAACACCGAGATCGCCAAC
AGCTGGGACCTGATCTTCAAGCCGGAGAACCTGTCCAAGCTCAAGGACTGCGGCGTCACCATTCTCGATACGCCCTCGGA
CATGATTCCGATCGCGCTGAACTACCTGGGCCTGGACCCGCACAGCACCGCGCCGGCCGAGATCGAAAAAGCCGCCGCGC
TGATCAAGACCATCCGCCCCTACGTGCAGAACTTCCACTCCAGCCAGTACGTGACTTCGCTGGCCAATGGCAACACCTGT
CTGGCGGTGGGCTGGTCGGGCGACATCATCCAGGCCCGCGACCGCGCGGCCGAAGCCGGCAACACCGTCAAGGTGGCGTA
TTCCATCCCGAAGGAAGGCGCGCCGCAGTGGTTCGACATGCTGGCGATCCCCAAGGATGCCAAACACCCGGAAAACGCTT
ACGCCTTCATCAATTATTTGCTGACGCCCAAGGTGGCGGCCGCCAACAGCAATTTCATCCACTACGCCAATCCGGTCAGC
GCCGCCACGCCGCTGGTGGATGCGGCCATCCGCAACGACCCCACCATCTACCCGCCGCCGGAGGTGACCGCCCGGATGTT
CACTTACGCGATCAATCCACCCGAGGTGGACCGCCTGTACACGCGGCTGTGGACCGAGATCAAGACGGGTCGCTGA

Upstream 100 bases:

>100_bases
CCAGAGGCCGCACGCTGTGCAAACGCGGATGCGGTGGGTAGGCTGGGTGCAGACCGGCCGCGATGCCGGGCCGCCCCACC
GCGCTGCCCTGGAGACCCAC

Downstream 100 bases:

>100_bases
CGCGGGCTCTCTCACATGCCCTGTGCGGCCCGTGCGAGGGCAGGACTAATAAAGTGCCAATGAGTCACAGCGGGGGCTGA
CCGGGTCATCACGCAATCTG

Product: periplasmic putrescine-binding protein; permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTLRLLALTLSITLLSGCGGGGAPGKADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVTYDVFDSDEMVETKLLAGGSD
YDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLANLDPDIMRRIAAQDPGNTYGVPYMIGTTGIGYNVDKLKAAFGNTEIAN
SWDLIFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKTIRPYVQNFHSSQYVTSLANGNTC
LAVGWSGDIIQARDRAAEAGNTVKVAYSIPKEGAPQWFDMLAIPKDAKHPENAYAFINYLLTPKVAAANSNFIHYANPVS
AATPLVDAAIRNDPTIYPPPEVTARMFTYAINPPEVDRLYTRLWTEIKTGR

Sequences:

>Translated_371_residues
MTLRLLALTLSITLLSGCGGGGAPGKADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVTYDVFDSDEMVETKLLAGGSD
YDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLANLDPDIMRRIAAQDPGNTYGVPYMIGTTGIGYNVDKLKAAFGNTEIAN
SWDLIFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKTIRPYVQNFHSSQYVTSLANGNTC
LAVGWSGDIIQARDRAAEAGNTVKVAYSIPKEGAPQWFDMLAIPKDAKHPENAYAFINYLLTPKVAAANSNFIHYANPVS
AATPLVDAAIRNDPTIYPPPEVTARMFTYAINPPEVDRLYTRLWTEIKTGR
>Mature_370_residues
TLRLLALTLSITLLSGCGGGGAPGKADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVTYDVFDSDEMVETKLLAGGSDY
DVVVPTLNFFGRQIQAGVFLPLDKSKIPNLANLDPDIMRRIAAQDPGNTYGVPYMIGTTGIGYNVDKLKAAFGNTEIANS
WDLIFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKTIRPYVQNFHSSQYVTSLANGNTCL
AVGWSGDIIQARDRAAEAGNTVKVAYSIPKEGAPQWFDMLAIPKDAKHPENAYAFINYLLTPKVAAANSNFIHYANPVSA
ATPLVDAAIRNDPTIYPPPEVTARMFTYAINPPEVDRLYTRLWTEIKTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=367, Percent_Identity=52.0435967302452, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787367, Length=340, Percent_Identity=33.2352941176471, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787711, Length=315, Percent_Identity=27.6190476190476, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40273; Mature: 40142

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLLALTLSITLLSGCGGGGAPGKADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVT
CEEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHCCCCCEEE
YDVFDSDEMVETKLLAGGSDYDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLANLDPDIMR
EEECCCCHHHHHHHCCCCCCCEEEECCHHHCCHHHCCCEEEECCHHHCCCCCCCCHHHHH
RIAAQDPGNTYGVPYMIGTTGIGYNVDKLKAAFGNTEIANSWDLIFKPENLSKLKDCGVT
HHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEE
ILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKTIRPYVQNFHSSQYVTSLANGNTC
EECCCCCCEEHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEE
LAVGWSGDIIQARDRAAEAGNTVKVAYSIPKEGAPQWFDMLAIPKDAKHPENAYAFINYL
EEECCCCCEEHHHHHHHHCCCEEEEEEECCCCCCCHHHHHEECCCCCCCCCHHHHHHHHH
LTPKVAAANSNFIHYANPVSAATPLVDAAIRNDPTIYPPPEVTARMFTYAINPPEVDRLY
HCCCEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHCEEEEEEECCCCHHHHHH
TRLWTEIKTGR
HHHHHHHCCCC
>Mature Secondary Structure 
TLRLLALTLSITLLSGCGGGGAPGKADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVT
EEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHCCCCCEEE
YDVFDSDEMVETKLLAGGSDYDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLANLDPDIMR
EEECCCCHHHHHHHCCCCCCCEEEECCHHHCCHHHCCCEEEECCHHHCCCCCCCCHHHHH
RIAAQDPGNTYGVPYMIGTTGIGYNVDKLKAAFGNTEIANSWDLIFKPENLSKLKDCGVT
HHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEE
ILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKTIRPYVQNFHSSQYVTSLANGNTC
EECCCCCCEEHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEE
LAVGWSGDIIQARDRAAEAGNTVKVAYSIPKEGAPQWFDMLAIPKDAKHPENAYAFINYL
EEECCCCCEEHHHHHHHHCCCEEEEEEECCCCCCCHHHHHEECCCCCCCCCHHHHHHHHH
LTPKVAAANSNFIHYANPVSAATPLVDAAIRNDPTIYPPPEVTARMFTYAINPPEVDRLY
HCCCEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHCEEEEEEECCCCHHHHHH
TRLWTEIKTGR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]