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The map label for this gene is puuB [H]

Identifier: 66768088

GI number: 66768088

Start: 2148246

End: 2149559

Strand: Reverse

Name: puuB [H]

Synonym: XC_1767

Alternate gene names: 66768088

Gene position: 2149559-2148246 (Counterclockwise)

Preceding gene: 66768089

Following gene: 66768087

Centisome position: 41.75

GC content: 72.15

Gene sequence:

>1314_bases
ATGGCCACCGCCTTGCCCGCCGGCTCGACCGACGCACCGGGCAACGGGTATCCCGACAGCTGGTATGCGCGCAGCACGCC
GCCCTTGCCTGCGCAGGCGCCGCTGCACGGTGCGCAGCGCAGCGATGTCTGCGTGCTCGGCGCCGGCTACACCGGGCTCA
CCGCGGCGCTGGCGCTGGCCGAGGCCGGCTACCAGGTCACCGTGCTGGAGGCCCGGCGGATTGGCTGGGGCGCCTCCGGG
CGCAACGGCGGCCAGGCCATCGTGGGCTATGGCTGCGAACAGGACACGCTGGAAGCGCTGGTCGGCGACCGCGATGCACG
CCTGCTGTTCGACTATTCGCGCGATGGCATGCGGCTGCTGCGCGAACGCATCGCGCGCCACGGCATTGCCTGCGATTGGC
GCGACGGCCACGCGCACGTGCCGTTGAAGCCACGCCAGGTGCAGGCCCTGCAGCGCGGCATCGTGCGCATGGCCGAACGC
TATGACTATCCACTGCAGTGGTGGGACCGCAGCCAACTGCGTGCGGAGCTGGACAGCCCGCTCTATCTCGGCGCCATGTT
CGACCCGGCCAGCGGGCACCTGCAGCCGCTGGCCTATGCGCTGGGGCTGGCCCGCGCAGCGCTGGCCGCCGGTGTGCGCA
TCTACGAGCACAGCGCGGTGACGCGGGTGCAACAGGGCGAGCGAGTCGTGCTGCACACCGACCAGGGCAGCGTCTCGGCG
GAGTTCGGTGTGCTTGCCGGCAACGCGCTGCTGCGCGGCGTCGCCGCACCGCTGGAGCGCCGCATCATGCCGGTGGGCAC
CTACGTCGGTGCCACCCCGCCGCTGGGCGCGGCGCGGGCCCGCCGCCTGATCCGCAACGACATGGCGGTGGCGGATACCA
ACTGGGCCCTGGACTACTACCGGCTCAGTGCCGACCACCGGCTGCTGTTCGGCGGGCGGGCCAGCTATTCGTCGCTGCCC
CCGCCGGGGCTGGCGCGGCTGATGACCCGCCGCATGCACACCGTATTCCCGCAACTGCGCGACGTGCCGCTGGAAACAGT
CTGGGGCGGCTACGTGGACATCTCGGCCAACCGGGCGCCGCACTGGGGCCGGCTGGCCCCGAACCTGTATTTCGCACAGG
GCTTTTCCGGCCACGGGGTAGCTGCCACCGGGCTGGCCGGCCAGGTCATCGCCGAGGCCATCACCGGCCAGAGCCAGCGC
CTGGACGTGTTCGCCCGCATTCCGCACGCGCCCTTTCCCGGCGGCCGGCTGCTGCGTACCCCGCTGCTGATGGCGGCGAT
GAGCTGGTACCGGCTGCGCGATGCGCTGTGGTGA

Upstream 100 bases:

>100_bases
AGTTCCAGCATGTGTTCGGCCAGCAGAAGCAGCTCGAGCTGCTCGACTATCAGGCGCTGGTGCCGGACCTGGACTACGCC
CGGTATCTGCGGACGGTCTG

Downstream 100 bases:

>100_bases
GTGCGACGCAGTGCTCAAGTTCTGCCGAACCGAGCCGTTAGCTGACCTGACGGTTCGATCCAGACGGCTGACCGATGTCC
CCAGGCACCCTACATCGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MATALPAGSTDAPGNGYPDSWYARSTPPLPAQAPLHGAQRSDVCVLGAGYTGLTAALALAEAGYQVTVLEARRIGWGASG
RNGGQAIVGYGCEQDTLEALVGDRDARLLFDYSRDGMRLLRERIARHGIACDWRDGHAHVPLKPRQVQALQRGIVRMAER
YDYPLQWWDRSQLRAELDSPLYLGAMFDPASGHLQPLAYALGLARAALAAGVRIYEHSAVTRVQQGERVVLHTDQGSVSA
EFGVLAGNALLRGVAAPLERRIMPVGTYVGATPPLGAARARRLIRNDMAVADTNWALDYYRLSADHRLLFGGRASYSSLP
PPGLARLMTRRMHTVFPQLRDVPLETVWGGYVDISANRAPHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAITGQSQR
LDVFARIPHAPFPGGRLLRTPLLMAAMSWYRLRDALW

Sequences:

>Translated_437_residues
MATALPAGSTDAPGNGYPDSWYARSTPPLPAQAPLHGAQRSDVCVLGAGYTGLTAALALAEAGYQVTVLEARRIGWGASG
RNGGQAIVGYGCEQDTLEALVGDRDARLLFDYSRDGMRLLRERIARHGIACDWRDGHAHVPLKPRQVQALQRGIVRMAER
YDYPLQWWDRSQLRAELDSPLYLGAMFDPASGHLQPLAYALGLARAALAAGVRIYEHSAVTRVQQGERVVLHTDQGSVSA
EFGVLAGNALLRGVAAPLERRIMPVGTYVGATPPLGAARARRLIRNDMAVADTNWALDYYRLSADHRLLFGGRASYSSLP
PPGLARLMTRRMHTVFPQLRDVPLETVWGGYVDISANRAPHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAITGQSQR
LDVFARIPHAPFPGGRLLRTPLLMAAMSWYRLRDALW
>Mature_436_residues
ATALPAGSTDAPGNGYPDSWYARSTPPLPAQAPLHGAQRSDVCVLGAGYTGLTAALALAEAGYQVTVLEARRIGWGASGR
NGGQAIVGYGCEQDTLEALVGDRDARLLFDYSRDGMRLLRERIARHGIACDWRDGHAHVPLKPRQVQALQRGIVRMAERY
DYPLQWWDRSQLRAELDSPLYLGAMFDPASGHLQPLAYALGLARAALAAGVRIYEHSAVTRVQQGERVVLHTDQGSVSAE
FGVLAGNALLRGVAAPLERRIMPVGTYVGATPPLGAARARRLIRNDMAVADTNWALDYYRLSADHRLLFGGRASYSSLPP
PGLARLMTRRMHTVFPQLRDVPLETVWGGYVDISANRAPHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAITGQSQRL
DVFARIPHAPFPGGRLLRTPLLMAAMSWYRLRDALW

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=422, Percent_Identity=43.1279620853081, Blast_Score=332, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47498; Mature: 47367

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATALPAGSTDAPGNGYPDSWYARSTPPLPAQAPLHGAQRSDVCVLGAGYTGLTAALALA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
EAGYQVTVLEARRIGWGASGRNGGQAIVGYGCEQDTLEALVGDRDARLLFDYSRDGMRLL
HCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEECCCCHHHHH
RERIARHGIACDWRDGHAHVPLKPRQVQALQRGIVRMAERYDYPLQWWDRSQLRAELDSP
HHHHHHCCCEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCC
LYLGAMFDPASGHLQPLAYALGLARAALAAGVRIYEHSAVTRVQQGERVVLHTDQGSVSA
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCCCCC
EFGVLAGNALLRGVAAPLERRIMPVGTYVGATPPLGAARARRLIRNDMAVADTNWALDYY
CHHEEEHHHHHHHHHHHHHHCEEEECHHCCCCCCCCHHHHHHHHHHCCEEECCCCEEEEE
RLSADHRLLFGGRASYSSLPPPGLARLMTRRMHTVFPQLRDVPLETVWGGYVDISANRAP
EECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCC
HWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAITGQSQRLDVFARIPHAPFPGGRLLRT
CCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCHHEEHEEECCCCCCCCCHHHHH
PLLMAAMSWYRLRDALW
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATALPAGSTDAPGNGYPDSWYARSTPPLPAQAPLHGAQRSDVCVLGAGYTGLTAALALA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
EAGYQVTVLEARRIGWGASGRNGGQAIVGYGCEQDTLEALVGDRDARLLFDYSRDGMRLL
HCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEECCCCHHHHH
RERIARHGIACDWRDGHAHVPLKPRQVQALQRGIVRMAERYDYPLQWWDRSQLRAELDSP
HHHHHHCCCEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCC
LYLGAMFDPASGHLQPLAYALGLARAALAAGVRIYEHSAVTRVQQGERVVLHTDQGSVSA
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCCCCC
EFGVLAGNALLRGVAAPLERRIMPVGTYVGATPPLGAARARRLIRNDMAVADTNWALDYY
CHHEEEHHHHHHHHHHHHHHCEEEECHHCCCCCCCCHHHHHHHHHHCCEEECCCCEEEEE
RLSADHRLLFGGRASYSSLPPPGLARLMTRRMHTVFPQLRDVPLETVWGGYVDISANRAP
EECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCC
HWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAITGQSQRLDVFARIPHAPFPGGRLLRT
CCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCHHEEHEEECCCCCCCCCHHHHH
PLLMAAMSWYRLRDALW
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]