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The map label for this gene is puuA [H]

Identifier: 66768089

GI number: 66768089

Start: 2149559

End: 2150938

Strand: Reverse

Name: puuA [H]

Synonym: XC_1768

Alternate gene names: 66768089

Gene position: 2150938-2149559 (Counterclockwise)

Preceding gene: 66768100

Following gene: 66768088

Centisome position: 41.78

GC content: 68.26

Gene sequence:

>1380_bases
ATGGCAACCGAAACCCGTGTCGCGTCCCTGCCCAGCATCGATGCCGCCGCCCTGGCCGCGATCGAGGGCTGTGCGCTGGT
GGACCTGCTGCTGCCCGATACCAACGGCGTGTTGCGCGGCAAGCGTGTCACCCGAGAGGCGCTCGACAAGGTCTACCGCG
ACGGCGTGTGCCTGCCGATGTCGCTGATCGCCACCGACATCACCGGCAATACGGTGGAAGAAACCGGCCTGGGCTACGCC
ATTGGCGATGCCGATCGCATCTGCCGCCCGATCGCCGGCTCGCTCCGCCCGGTGCCATGGGCGCCGCAACCGATGGCGCA
GCTGTTGCTGGCAATGCACACCCCCAACGGTGCCTTGTTCGAGGTCGCGCCGCGCGCGGTGCTGCAGCGCGTGGTGCAAG
GATTCGCCGCGCTGGGCCTGACGCCGGTGATCGCCGTGGAGCTGGAGTTCTACCTGTTCGACGCCATGGCCGATGCCCAG
GGCCGTCCGCAGACGCCGCGCGATGCACACACCGGCGAGCGCAACACCAGCACCCAGGTGTATGCATTGCAGGATCTGGA
CGACCACCGCAGCTTCACCGATGCCGTCACTGCGGCGTGCCGGCAGCAAGGCATTCCGGCCGATACCGCGGTGGCCGAAT
ACGCGCCCGGGCAGTTCGAGATCAACCTGCAGCACCGCGCCGATGCGGTGGCCGCATGCGACGAGGCGCTGCTGCTCAAG
CGCACCATCAAGGCGATCGCGCAGCAGCAACACCTGTTGGCCAGTTTCATGGCCAAGCCATTCGCCGGCCAGGCCGGCAG
CGGGCTGCACGTGCACGTCAGCCTGCTCGATGCCGCCGGCCACAACGTGCTGCACGGCACAGCAGATGCGCCGGCCGCCG
CCCTGCGCCATGCCATTGCCGGGTTGCAACGGCATGCGGACGCATCGCTGCTGCTGTTCGCGCCGCATGCCAACAGTTAC
CGCCGTTTCGTGCCGAATGCGTTCGTGCCGCTGGACGCCAGCTGGGGCTTCAACAACCGCACCGTGGCCTTGCGCATCCC
GCACAGCGATGCGCACAACACCCGTATCGAACACCGCATCGCCGGCGCCGATGCCAACCCCTACCTGGTGGCGGCGGCCG
TGTTGGCCGCGATGCTGGACGGCTTGCAGCACCCCGGCGACCCCACCGCCCCGGTGCAGGGCAACGCCTACGCGCAGGCG
ACCTTCACCCCGCGCACCTGGCAGGGTGCGGTGGATGCGTTCCTGCGCAGCGACTTCATCGCCACCCAATTGGGCACGCA
GTTCCAGCATGTGTTCGGCCAGCAGAAGCAGCTCGAGCTGCTCGACTATCAGGCGCTGGTGCCGGACCTGGACTACGCCC
GGTATCTGCGGACGGTCTGA

Upstream 100 bases:

>100_bases
TTAGCGCGCCGCCCGGTGCATTGCCACCGCGCGCACGCAACTCCTGCGCCTGCTCACGCCTGCTTTGCTACGCTGGCCCG
CGATTCCACAGGTGTGGCCC

Downstream 100 bases:

>100_bases
TGGCCACCGCCTTGCCCGCCGGCTCGACCGACGCACCGGGCAACGGGTATCCCGACAGCTGGTATGCGCGCAGCACGCCG
CCCTTGCCTGCGCAGGCGCC

Product: glutamine synthase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MATETRVASLPSIDAAALAAIEGCALVDLLLPDTNGVLRGKRVTREALDKVYRDGVCLPMSLIATDITGNTVEETGLGYA
IGDADRICRPIAGSLRPVPWAPQPMAQLLLAMHTPNGALFEVAPRAVLQRVVQGFAALGLTPVIAVELEFYLFDAMADAQ
GRPQTPRDAHTGERNTSTQVYALQDLDDHRSFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEALLLK
RTIKAIAQQQHLLASFMAKPFAGQAGSGLHVHVSLLDAAGHNVLHGTADAPAAALRHAIAGLQRHADASLLLFAPHANSY
RRFVPNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRIAGADANPYLVAAAVLAAMLDGLQHPGDPTAPVQGNAYAQA
TFTPRTWQGAVDAFLRSDFIATQLGTQFQHVFGQQKQLELLDYQALVPDLDYARYLRTV

Sequences:

>Translated_459_residues
MATETRVASLPSIDAAALAAIEGCALVDLLLPDTNGVLRGKRVTREALDKVYRDGVCLPMSLIATDITGNTVEETGLGYA
IGDADRICRPIAGSLRPVPWAPQPMAQLLLAMHTPNGALFEVAPRAVLQRVVQGFAALGLTPVIAVELEFYLFDAMADAQ
GRPQTPRDAHTGERNTSTQVYALQDLDDHRSFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEALLLK
RTIKAIAQQQHLLASFMAKPFAGQAGSGLHVHVSLLDAAGHNVLHGTADAPAAALRHAIAGLQRHADASLLLFAPHANSY
RRFVPNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRIAGADANPYLVAAAVLAAMLDGLQHPGDPTAPVQGNAYAQA
TFTPRTWQGAVDAFLRSDFIATQLGTQFQHVFGQQKQLELLDYQALVPDLDYARYLRTV
>Mature_458_residues
ATETRVASLPSIDAAALAAIEGCALVDLLLPDTNGVLRGKRVTREALDKVYRDGVCLPMSLIATDITGNTVEETGLGYAI
GDADRICRPIAGSLRPVPWAPQPMAQLLLAMHTPNGALFEVAPRAVLQRVVQGFAALGLTPVIAVELEFYLFDAMADAQG
RPQTPRDAHTGERNTSTQVYALQDLDDHRSFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEALLLKR
TIKAIAQQQHLLASFMAKPFAGQAGSGLHVHVSLLDAAGHNVLHGTADAPAAALRHAIAGLQRHADASLLLFAPHANSYR
RFVPNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRIAGADANPYLVAAAVLAAMLDGLQHPGDPTAPVQGNAYAQAT
FTPRTWQGAVDAFLRSDFIATQLGTQFQHVFGQQKQLELLDYQALVPDLDYARYLRTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=421, Percent_Identity=24.9406175771971, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081870, Length=433, Percent_Identity=44.3418013856813, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1790301, Length=300, Percent_Identity=28.3333333333333, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49264; Mature: 49133

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATETRVASLPSIDAAALAAIEGCALVDLLLPDTNGVLRGKRVTREALDKVYRDGVCLPM
CCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCH
SLIATDITGNTVEETGLGYAIGDADRICRPIAGSLRPVPWAPQPMAQLLLAMHTPNGALF
HHHHCCCCCCCHHHCCCCEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEE
EVAPRAVLQRVVQGFAALGLTPVIAVELEFYLFDAMADAQGRPQTPRDAHTGERNTSTQV
HHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
YALQDLDDHRSFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEALLLK
EEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHH
RTIKAIAQQQHLLASFMAKPFAGQAGSGLHVHVSLLDAAGHNVLHGTADAPAAALRHAIA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHH
GLQRHADASLLLFAPHANSYRRFVPNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRI
HHHHCCCCEEEEECCCCCCHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCHHHEE
AGADANPYLVAAAVLAAMLDGLQHPGDPTAPVQGNAYAQATFTPRTWQGAVDAFLRSDFI
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
ATQLGTQFQHVFGQQKQLELLDYQALVPDLDYARYLRTV
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
ATETRVASLPSIDAAALAAIEGCALVDLLLPDTNGVLRGKRVTREALDKVYRDGVCLPM
CCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCH
SLIATDITGNTVEETGLGYAIGDADRICRPIAGSLRPVPWAPQPMAQLLLAMHTPNGALF
HHHHCCCCCCCHHHCCCCEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEE
EVAPRAVLQRVVQGFAALGLTPVIAVELEFYLFDAMADAQGRPQTPRDAHTGERNTSTQV
HHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
YALQDLDDHRSFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEALLLK
EEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHH
RTIKAIAQQQHLLASFMAKPFAGQAGSGLHVHVSLLDAAGHNVLHGTADAPAAALRHAIA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHH
GLQRHADASLLLFAPHANSYRRFVPNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRI
HHHHCCCCEEEEECCCCCCHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCHHHEE
AGADANPYLVAAAVLAAMLDGLQHPGDPTAPVQGNAYAQATFTPRTWQGAVDAFLRSDFI
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
ATQLGTQFQHVFGQQKQLELLDYQALVPDLDYARYLRTV
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]