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The map label for this gene is fruB [H]

Identifier: 66768067

GI number: 66768067

Start: 2110108

End: 2112624

Strand: Reverse

Name: fruB [H]

Synonym: XC_1744

Alternate gene names: 66768067

Gene position: 2112624-2110108 (Counterclockwise)

Preceding gene: 66768069

Following gene: 66768066

Centisome position: 41.03

GC content: 71.71

Gene sequence:

>2517_bases
TTGTCCTCTCCGTCGATTGCCCCCGTCACCCCCGACCTGGTGCGCCTGCGCGCCACTGCCCGCGACAAGGACGACGCCAT
TGCCCAGGCCGCCCAGCTGCTGGTGGCAGCCGGATGCGTGGCGCCGGGCTACGACGCCAGCATGCGGCGGCGCGAAGGCC
TGGCGAATACTTTCCTGGGCCACGGCCTGGCGATTCCGCACGGCGTGGGCGAAGACCGGCACCTGGTGCGCCGCGACGGC
ATCGCGGTGCTGCAGCTGCCCGAGGGCGTGGAATGGAACCCCGGCCAGACCACCCGCCTGGTGGTGGGCATCGCCGCCCA
GTCCGACACCCACATCACCCTGCTGCGCCGGCTGACCCGCCTGATCCAGGATCCGGCCCAGCTGGAAGCGCTGTTCACCA
CCGACGACCCGGCCGTGATCGTGGCCGCGCTGACCGGCGACCGCGCACCCGACACCAGCGCCGCCCCGGCCACCGACCTG
GCCGAACGGTTCGAATGGACCATCGCCTACCCCAGCGGCCTGCACGCGCGCCCGGCCACTCGCTGGGCGGAGACCGCGCG
CGGCTTTTCGGCCCGCGCCCAGGTCCGCGCCGGTGACCAGGCCGCCGATGCCAAGAGCCTGGTCGGCCTGCTGCAACTGG
GCCTGCGCGCCGGCGACAGCATTACGGTCTCAGCGAAAGGCAGCGATGCCCCCGCCCTGCTCAAGCGCCTGCGCGCGGTG
ATGGACAGCCTGACCGCGCAGGAAAAGGCCGATGCCGAACGTGCCGCGCAACGCCGCGCCGCACCGGTCATCGGCTGGAC
CCCGCCGCAGGCGCAGCCGGCCATCGTCGGCATCGGCGCCAGCCCGGGCGTGGCAATCGGCATCGTGCACCGCCTGCGTG
CCGCGCAGACCGAGGTGGCCGACCAGCCGATCGGCCTGGGCGACGGCGGCGTGCTGCTGCATGACGCCCTCACCCGCACC
CGCCAGCAGCTGGCCGCCATCCAGGACGACACCCAGCGCCGCCTGGGCGCCTCGGACGCGGCGATCTTCAAGGCCCAGGC
CGAGCTGCTCAACGACACCGACCTGATCACCCGCACCTGCCAGCTGATGGTCGAAGGCCACGGTGTGGCGTGGTCGTGGC
ACCAGGCGGTGGAACAGATCGCCTCCGGCCTCGCGGCGCTGGGCAACCCGGTGCTGGCCGGCCGCGCCGCTGACCTGCGC
GACGTGGGCCGCCGTGTGCTGGCCCAACTCGACCCGGCAGCGGCCGGCGCCGGCCTCACCGACCTGCCCGAGCAGCCCTG
CATCCTGCTCGCCGGCGACCTGTCGCCGTCGGACACCGCCAACCTCGACACCGATTGCGTGCTTGGCCTGGCCACCGCCC
AGGGTGGGCCGACCTCGCACACCGCGATCCTGTCGCGCACGCTCGGCCTGCCGGCGCTGGTCGCGGCTGGCGGCCAGTTG
CTGGATATCGAAGACGGCGTCACCGCCATCATCGACGGCAGCAGCGGGCGGCTCTACATCAACCCGTCAGAACAGGATCT
GGACGCCGCCCGCACGCATATCGCCGAGCAGCAGGCCATCCGCGAGCGCGAAGCCGCCCAGCGCGCCCTGCCCGCCGAAA
CCACCGACGGCCACCACATCGACATCGGCGCCAACGTCAACCTGCCTGAGCAGGTCGCCATGGCCTTGACCCAGGGCGCC
GAAGGCGTGGGCCTGATGCGTACCGAATTCCTGTTCCTTGAGCGCGGCAGCACGCCCACCGAGGACGAGCAGTACCAGAC
ATACCTGGCCATGGCACGGGCCCTGGACGGCCGCCCGCTGATCGTGCGTGCGCTGGACATCGGCGGCGACAAGCAGGTGG
CGCACCTGGAGCTGCCGCACGAAGAAAACCCGTTCCTGGGCGTGCGTGGCGCGCGCCTGCTGCTGCGCCGCCCCGACCTG
CTCGAGCCACAGCTGCGGGCGCTGTATCGCGCGGCCAAGGACGGCGCACGGCTGTCGATCATGTTCCCGATGATCACCTC
GGTGCCGGAGCTGATCAGCCTGCGCGAGATCTGCGCACGCATCCGCGCCGAACTCGACGCACCGGAGCTGCCGATCGGCA
TCATGATCGAAGTGCCCGCCGCCGCCGCCCAGGCCGATGTGCTGGCGCGCCATGCGGATTTCTTCTCGATCGGCACCAAC
GACCTCACCCAATACGTGCTGGCAATCGACCGCCAGAACCCGGAACTGGCCGCCGAAGCCGACAGCCTGCATCCGGCGGT
GCTGCGCATGATCCGCAGCACGATTGACGGCGCGCGCAAGCACGACCGCTGGGTCGGGGTGTGCGGCGGTCTGGCCGGCG
ACCCGTTCGGCGCCAGCCTGCTGGCCGGGCTGGGCGTGCAGGAATTGTCGATGACGCCCAACGACATTCCGGCGGTCAAG
GCGCGGCTGCGTGGCAGGGCGCTGAGCGCCTTGCAGCAGCTGGCCGAGCAGGCGCTGCAGTGCGAAACCGCAGAGCAGGT
GCGTGCGCTTGAAGCACAGCGCGAGGGCCAGGCATGA

Upstream 100 bases:

>100_bases
CCCGAAAGCACTACAGGGTTTTTGTGCACCGCACATTGACCTGGACCGTGGGAACGTTCCCACTATACTTCCCAGCCGTT
CCCCACCCCCGGAGCCCGCC

Downstream 100 bases:

>100_bases
GCCTGCAGGCCATCACCGTCACGCTGAATCCGGCGATCGACCAGACCATCCAGCTCGATCGCCTGCAACCTGGCGCCGTG
CACCGCGCCAGCAGCGTGCG

Product: multiphosphoryl transfer protein

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 838; Mature: 837

Protein sequence:

>838_residues
MSSPSIAPVTPDLVRLRATARDKDDAIAQAAQLLVAAGCVAPGYDASMRRREGLANTFLGHGLAIPHGVGEDRHLVRRDG
IAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFTTDDPAVIVAALTGDRAPDTSAAPATDL
AERFEWTIAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAKGSDAPALLKRLRAV
MDSLTAQEKADAERAAQRRAAPVIGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPIGLGDGGVLLHDALTRT
RQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLR
DVGRRVLAQLDPAAAGAGLTDLPEQPCILLAGDLSPSDTANLDTDCVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
LDIEDGVTAIIDGSSGRLYINPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPEQVAMALTQGA
EGVGLMRTEFLFLERGSTPTEDEQYQTYLAMARALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDL
LEPQLRALYRAAKDGARLSIMFPMITSVPELISLREICARIRAELDAPELPIGIMIEVPAAAAQADVLARHADFFSIGTN
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIDGARKHDRWVGVCGGLAGDPFGASLLAGLGVQELSMTPNDIPAVK
ARLRGRALSALQQLAEQALQCETAEQVRALEAQREGQA

Sequences:

>Translated_838_residues
MSSPSIAPVTPDLVRLRATARDKDDAIAQAAQLLVAAGCVAPGYDASMRRREGLANTFLGHGLAIPHGVGEDRHLVRRDG
IAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFTTDDPAVIVAALTGDRAPDTSAAPATDL
AERFEWTIAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAKGSDAPALLKRLRAV
MDSLTAQEKADAERAAQRRAAPVIGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPIGLGDGGVLLHDALTRT
RQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLR
DVGRRVLAQLDPAAAGAGLTDLPEQPCILLAGDLSPSDTANLDTDCVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
LDIEDGVTAIIDGSSGRLYINPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPEQVAMALTQGA
EGVGLMRTEFLFLERGSTPTEDEQYQTYLAMARALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDL
LEPQLRALYRAAKDGARLSIMFPMITSVPELISLREICARIRAELDAPELPIGIMIEVPAAAAQADVLARHADFFSIGTN
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIDGARKHDRWVGVCGGLAGDPFGASLLAGLGVQELSMTPNDIPAVK
ARLRGRALSALQQLAEQALQCETAEQVRALEAQREGQA
>Mature_837_residues
SSPSIAPVTPDLVRLRATARDKDDAIAQAAQLLVAAGCVAPGYDASMRRREGLANTFLGHGLAIPHGVGEDRHLVRRDGI
AVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFTTDDPAVIVAALTGDRAPDTSAAPATDLA
ERFEWTIAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAKGSDAPALLKRLRAVM
DSLTAQEKADAERAAQRRAAPVIGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPIGLGDGGVLLHDALTRTR
QQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRD
VGRRVLAQLDPAAAGAGLTDLPEQPCILLAGDLSPSDTANLDTDCVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQLL
DIEDGVTAIIDGSSGRLYINPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPEQVAMALTQGAE
GVGLMRTEFLFLERGSTPTEDEQYQTYLAMARALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLL
EPQLRALYRAAKDGARLSIMFPMITSVPELISLREICARIRAELDAPELPIGIMIEVPAAAAQADVLARHADFFSIGTND
LTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIDGARKHDRWVGVCGGLAGDPFGASLLAGLGVQELSMTPNDIPAVKA
RLRGRALSALQQLAEQALQCETAEQVRALEAQREGQA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=569, Percent_Identity=39.1915641476274, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788726, Length=682, Percent_Identity=32.8445747800587, Blast_Score=298, Evalue=1e-81,
Organism=Escherichia coli, GI48994992, Length=704, Percent_Identity=32.5284090909091, Blast_Score=296, Evalue=5e-81,
Organism=Escherichia coli, GI1789193, Length=499, Percent_Identity=35.4709418837675, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI1788494, Length=129, Percent_Identity=44.9612403100775, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1787994, Length=415, Percent_Identity=26.0240963855422, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI226510935, Length=358, Percent_Identity=26.2569832402235, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1790027, Length=149, Percent_Identity=33.5570469798658, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88763; Mature: 88632

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSIAPVTPDLVRLRATARDKDDAIAQAAQLLVAAGCVAPGYDASMRRREGLANTFLG
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HGLAIPHGVGEDRHLVRRDGIAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTR
CCCCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
LIQDPAQLEALFTTDDPAVIVAALTGDRAPDTSAAPATDLAERFEWTIAYPSGLHARPAT
HHCCHHHEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCHH
RWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAKGSDAPALLKRLRAV
HHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
MDSLTAQEKADAERAAQRRAAPVIGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVA
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
DQPIGLGDGGVLLHDALTRTRQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
QLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRDVGRRVLAQLDPAAAGAGLT
HHHEECCCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCHHCCCCCC
DLPEQPCILLAGDLSPSDTANLDTDCVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
CCCCCCEEEEECCCCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCE
LDIEDGVTAIIDGSSGRLYINPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHI
EEECCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DIGANVNLPEQVAMALTQGAEGVGLMRTEFLFLERGSTPTEDEQYQTYLAMARALDGRPL
EECCCCCCHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCE
IVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLLEPQLRALYRAAKDGARLSI
EEEEEECCCCCEEEEEECCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
MFPMITSVPELISLREICARIRAELDAPELPIGIMIEVPAAAAQADVLARHADFFSIGTN
EHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCH
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIDGARKHDRWVGVCGGLAGDPFGASL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHH
LAGLGVQELSMTPNDIPAVKARLRGRALSALQQLAEQALQCETAEQVRALEAQREGQA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSPSIAPVTPDLVRLRATARDKDDAIAQAAQLLVAAGCVAPGYDASMRRREGLANTFLG
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HGLAIPHGVGEDRHLVRRDGIAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTR
CCCCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
LIQDPAQLEALFTTDDPAVIVAALTGDRAPDTSAAPATDLAERFEWTIAYPSGLHARPAT
HHCCHHHEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCHH
RWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAKGSDAPALLKRLRAV
HHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
MDSLTAQEKADAERAAQRRAAPVIGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVA
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
DQPIGLGDGGVLLHDALTRTRQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
QLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRDVGRRVLAQLDPAAAGAGLT
HHHEECCCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCHHCCCCCC
DLPEQPCILLAGDLSPSDTANLDTDCVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
CCCCCCEEEEECCCCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCE
LDIEDGVTAIIDGSSGRLYINPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHI
EEECCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DIGANVNLPEQVAMALTQGAEGVGLMRTEFLFLERGSTPTEDEQYQTYLAMARALDGRPL
EECCCCCCHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCE
IVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLLEPQLRALYRAAKDGARLSI
EEEEEECCCCCEEEEEECCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
MFPMITSVPELISLREICARIRAELDAPELPIGIMIEVPAAAAQADVLARHADFFSIGTN
EHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCH
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIDGARKHDRWVGVCGGLAGDPFGASL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHH
LAGLGVQELSMTPNDIPAVKARLRGRALSALQQLAEQALQCETAEQVRALEAQREGQA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496537; 12024217; 1655739 [H]