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The map label for this gene is greB [H]

Identifier: 66767879

GI number: 66767879

Start: 1871600

End: 1872106

Strand: Reverse

Name: greB [H]

Synonym: XC_1555

Alternate gene names: 66767879

Gene position: 1872106-1871600 (Counterclockwise)

Preceding gene: 66767881

Following gene: 66767877

Centisome position: 36.36

GC content: 66.07

Gene sequence:

>507_bases
ATGAGCCGCTGGCGTCCCCCTGCCGAAAAAAGCACTGCGTTGATCACGCCGGAAGGCCATGCGCGGCTCAAGGCCGAACT
GGACGAGCTGTGGCGCCTGCGTCGGCCCGAGGTGGTGCGCGCGCTCGCGGCGGCCGCAGCCGAGGGCGACCGCTCGGAAA
ACGCCGAGTACACCTATCGCAAGAAACAGCTGGGCGAGATCGATCGCCGCGTGCGTTACCTCAGCAAGCGGCTGGAAGCG
CTGCGCGTAGTGGATACCGCGCCGACGGATCCGCAGGCGGTGTTCTTTGGTGCGCAGGTTGAGCTGGAGGATGCCGACAG
CGGCGAGCTGCTGCGTTACCGCATCGTGGGGCCGGATGAGACCGACGCCGGGCGCGGCTGGATCAGCATCGATTCGCCAT
TGGCGCGCGCACTGCTGAAAAAGCGCGTGGACGATGAGTTCGAGGCACAACTCCCGTCGGGCAAGCACACCTTTGTGGTG
GTGGTGGTGGAGTACGCGGCAACTTAG

Upstream 100 bases:

>100_bases
GTCCGTGTCCCAAGGCGCAAAGCTTCGCCGGGCGGATGCGGATGCGCAAGCGCGCCATGCGCGCTAGCATCGCGCCCCCA
TCCTGCCCTGTTGGTCGCCC

Downstream 100 bases:

>100_bases
GTGGTGTGGGCGTGCGTGAGGCAATCCGGGTGGTTTGGATCCTTGCCTGCTGGTGCCGATGCGCGCGGTTGGAGGCGTGT
GGTTCTGGGGTAACGCCTCA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSRWRPPAEKSTALITPEGHARLKAELDELWRLRRPEVVRALAAAAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRLEA
LRVVDTAPTDPQAVFFGAQVELEDADSGELLRYRIVGPDETDAGRGWISIDSPLARALLKKRVDDEFEAQLPSGKHTFVV
VVVEYAAT

Sequences:

>Translated_168_residues
MSRWRPPAEKSTALITPEGHARLKAELDELWRLRRPEVVRALAAAAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRLEA
LRVVDTAPTDPQAVFFGAQVELEDADSGELLRYRIVGPDETDAGRGWISIDSPLARALLKKRVDDEFEAQLPSGKHTFVV
VVVEYAAT
>Mature_167_residues
SRWRPPAEKSTALITPEGHARLKAELDELWRLRRPEVVRALAAAAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRLEAL
RVVDTAPTDPQAVFFGAQVELEDADSGELLRYRIVGPDETDAGRGWISIDSPLARALLKKRVDDEFEAQLPSGKHTFVVV
VVEYAAT

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=53.5483870967742, Blast_Score=156, Evalue=8e-40,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=40.1315789473684, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18917; Mature: 18785

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRWRPPAEKSTALITPEGHARLKAELDELWRLRRPEVVRALAAAAAEGDRSENAEYTYR
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
KKQLGEIDRRVRYLSKRLEALRVVDTAPTDPQAVFFGAQVELEDADSGELLRYRIVGPDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEECCCCCCCEEEEEEECCCC
TDAGRGWISIDSPLARALLKKRVDDEFEAQLPSGKHTFVVVVVEYAAT
CCCCCCEEEECCHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
SRWRPPAEKSTALITPEGHARLKAELDELWRLRRPEVVRALAAAAAEGDRSENAEYTYR
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
KKQLGEIDRRVRYLSKRLEALRVVDTAPTDPQAVFFGAQVELEDADSGELLRYRIVGPDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEECCCCCCCEEEEEEECCCC
TDAGRGWISIDSPLARALLKKRVDDEFEAQLPSGKHTFVVVVVEYAAT
CCCCCCEEEECCHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]