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The map label for this gene is phoA [H]

Identifier: 66767845

GI number: 66767845

Start: 1827645

End: 1829351

Strand: Reverse

Name: phoA [H]

Synonym: XC_1519

Alternate gene names: 66767845

Gene position: 1829351-1827645 (Counterclockwise)

Preceding gene: 66767848

Following gene: 66767844

Centisome position: 35.53

GC content: 69.65

Gene sequence:

>1707_bases
ATGCCGATGCGTTACCGCCTGCCTGCCCTCGCCGCCCTGACCACGCTATGCGTGGCCGCCTGCGCTTCCACTGCCGGATC
CACCACCGCGGCGCCCGCCTCGGTCAAGGTGGACGTGCCACCGGTAACGCATCCGGCCGGCGAAACTCCGCAATGGTGGT
ACCGCTCCGGCGCCGCACGTGCGGCCGGCAACGGCGCGATGGCCGGCAAGGCACGCAACGTGGTCCTGTTCCTGGGCGAT
GGCATGAGCCTGACCACGGTGGCCGCCGCACGCATCCTGGACGGTCAGCGCAAGGGCGGCCCGGGCGAAGAGAATTTGCT
GTCGTGGGAGCAATTCCCCGCCACCGCCTTCAGCAAGACCTACAACACCGATTCGCAGACCCCGGATTCGGCCGGCACCA
TGACCGCGATCACCACCGGGGTGAAGTCGCACATGGGCGCGATCGGGGTCAGCAGCGGCAACCGCAGCGACTGTGCCGAC
AGCCTGGGCAAGGGCCTGCTGAGCTGGCTGCAACTGGCCGACAGCGCCGGCATGGCCACCGGTGTGGTCACCACCACCCG
TCTGACCCACGCCACGCCGGCGGCGACCTACGCGCATTCGCCGGACCGCAACTGGGAGAACGACACCGACCTGCCCGAGG
CCGCCCGCATCGCCGGCTGCCAGGACATCGCCCAGCAATTGCTCTCCACCGCACGCTTCGGGCGTGGCCCGCAGGTGGTG
CTCGGCGGCGGCCGCAGCCAGTTCCAGACCGTGCAGGAGCGCGACCCCGAGTACGACGACAAGGTCGGCCTGCGCCTGGA
CGGGCGCGACCTGGTGGCCGAGTGGAAGCAGTCCCACCCGCAAGGCGCCTACGTGTGGAACGAACAGCAATTGCAGGCCG
CCGCCGGCGCACCCGCGTTGCTGGGCCTGTTCGAGCCCGATCACATGCAGTTCGACCATGACCGCAACCGCACGCCGCAA
GGCGAGCCCAGCCTGACCGAGATGACCCGCACCGCGATCCAGTCGCTGTCGCGCGACCCGAACGGCTTCGTGCTGATGGT
CGAAGGCGGCCGTATCGATCACGCCAACCACGCGGGCAATGCCTACCGCGCGCTGGACGAGACCGTCTCGCTGTCGGATG
CAGTGCGCACCGCCGTGCAGACAGCGCCGCCGGACACGCTGATCATCGTCACCGCCGACCATTCGCACACGCTCAACTTT
GTCGGCTACCCGGTGCGTGGCAACCCGATCCTGGGCAAGGTGCGCGGCACCGGCGGCGAAGAAGGCGACCGCACCCAGCT
GGCCACCGATCTGGCCGGCCAGCCCTACACCACCCTCAGCTACGCCAATGGCCCCGGCCATACCGGCGCGACCAACGCAC
AGCCGGCCGGGCCGAAAAAGTATCCGCACGAGCCCAGCAGCAGCGAGCCGGCAAGCGGCCGCCCCGACCTGACCCACGTG
GATACCGAGGCGCCCAGCTACATGCAGGAATCGCTGGTACCGACCAAGTCTGAAAGCCACGGCGGCGAAGACGTCGGCAT
CTGGGCCACCGGCCCGGGCAGCGCGGCGTTCCGCGGCACGCTGGAAGAGAACGTGATCTACCACGTCATCGTCCAGGCCA
CCCCGCGTCTACGTGCGCGCCTGTGCAAGGCCGGCACCTGCGACAGCGCGGGTGTGCCGGTGAACCTGCCCGCACCCACC
ACCTTCGAGCGCGCGACCCAGCGCTGA

Upstream 100 bases:

>100_bases
AGGGCCGCCCTGCCCGCGAGGCCACGACTGGAACAGCGGCGTGTCATCAGTGCGACGGATGCATCCCGGCATAATGGGCA
TTGATTCAGTCACGAGTTCG

Downstream 100 bases:

>100_bases
TGGCCGAGCGCACCGCGCACGACCGGTGCGGTGCGCGGCTGGCAGCGGTCGGCGTCATGACGGGCGTGGCCTGACGTGGA
CGTGATGCAGGCAGCCGGGT

Product: alkaline phosphatase

Products: NA

Alternate protein names: High molecular weight phosphatase; H-AP [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MPMRYRLPALAALTTLCVAACASTAGSTTAAPASVKVDVPPVTHPAGETPQWWYRSGAARAAGNGAMAGKARNVVLFLGD
GMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKTYNTDSQTPDSAGTMTAITTGVKSHMGAIGVSSGNRSDCAD
SLGKGLLSWLQLADSAGMATGVVTTTRLTHATPAATYAHSPDRNWENDTDLPEAARIAGCQDIAQQLLSTARFGRGPQVV
LGGGRSQFQTVQERDPEYDDKVGLRLDGRDLVAEWKQSHPQGAYVWNEQQLQAAAGAPALLGLFEPDHMQFDHDRNRTPQ
GEPSLTEMTRTAIQSLSRDPNGFVLMVEGGRIDHANHAGNAYRALDETVSLSDAVRTAVQTAPPDTLIIVTADHSHTLNF
VGYPVRGNPILGKVRGTGGEEGDRTQLATDLAGQPYTTLSYANGPGHTGATNAQPAGPKKYPHEPSSSEPASGRPDLTHV
DTEAPSYMQESLVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRARLCKAGTCDSAGVPVNLPAPT
TFERATQR

Sequences:

>Translated_568_residues
MPMRYRLPALAALTTLCVAACASTAGSTTAAPASVKVDVPPVTHPAGETPQWWYRSGAARAAGNGAMAGKARNVVLFLGD
GMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKTYNTDSQTPDSAGTMTAITTGVKSHMGAIGVSSGNRSDCAD
SLGKGLLSWLQLADSAGMATGVVTTTRLTHATPAATYAHSPDRNWENDTDLPEAARIAGCQDIAQQLLSTARFGRGPQVV
LGGGRSQFQTVQERDPEYDDKVGLRLDGRDLVAEWKQSHPQGAYVWNEQQLQAAAGAPALLGLFEPDHMQFDHDRNRTPQ
GEPSLTEMTRTAIQSLSRDPNGFVLMVEGGRIDHANHAGNAYRALDETVSLSDAVRTAVQTAPPDTLIIVTADHSHTLNF
VGYPVRGNPILGKVRGTGGEEGDRTQLATDLAGQPYTTLSYANGPGHTGATNAQPAGPKKYPHEPSSSEPASGRPDLTHV
DTEAPSYMQESLVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRARLCKAGTCDSAGVPVNLPAPT
TFERATQR
>Mature_567_residues
PMRYRLPALAALTTLCVAACASTAGSTTAAPASVKVDVPPVTHPAGETPQWWYRSGAARAAGNGAMAGKARNVVLFLGDG
MSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKTYNTDSQTPDSAGTMTAITTGVKSHMGAIGVSSGNRSDCADS
LGKGLLSWLQLADSAGMATGVVTTTRLTHATPAATYAHSPDRNWENDTDLPEAARIAGCQDIAQQLLSTARFGRGPQVVL
GGGRSQFQTVQERDPEYDDKVGLRLDGRDLVAEWKQSHPQGAYVWNEQQLQAAAGAPALLGLFEPDHMQFDHDRNRTPQG
EPSLTEMTRTAIQSLSRDPNGFVLMVEGGRIDHANHAGNAYRALDETVSLSDAVRTAVQTAPPDTLIIVTADHSHTLNFV
GYPVRGNPILGKVRGTGGEEGDRTQLATDLAGQPYTTLSYANGPGHTGATNAQPAGPKKYPHEPSSSEPASGRPDLTHVD
TEAPSYMQESLVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRARLCKAGTCDSAGVPVNLPAPTT
FERATQR

Specific function: Has only a phosphomonoesterase activity [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted. Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=520, Percent_Identity=42.1153846153846, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI157266296, Length=520, Percent_Identity=41.9230769230769, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI157266292, Length=497, Percent_Identity=41.2474849094567, Blast_Score=358, Evalue=9e-99,
Organism=Homo sapiens, GI116734717, Length=473, Percent_Identity=42.0718816067653, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI294660770, Length=468, Percent_Identity=42.0940170940171, Blast_Score=342, Evalue=6e-94,
Organism=Homo sapiens, GI294660772, Length=436, Percent_Identity=39.4495412844037, Blast_Score=296, Evalue=5e-80,
Organism=Escherichia coli, GI48994877, Length=369, Percent_Identity=31.1653116531165, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320689, Length=354, Percent_Identity=31.9209039548023, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24651554, Length=529, Percent_Identity=39.6975425330813, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI21355149, Length=490, Percent_Identity=39.7959183673469, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI21358067, Length=469, Percent_Identity=41.5778251599147, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI21355151, Length=473, Percent_Identity=41.4376321353066, Blast_Score=301, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24657835, Length=514, Percent_Identity=38.1322957198444, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI85725200, Length=493, Percent_Identity=39.9594320486815, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI85815833, Length=493, Percent_Identity=39.9594320486815, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI19921912, Length=539, Percent_Identity=37.4768089053803, Blast_Score=289, Evalue=5e-78,
Organism=Drosophila melanogaster, GI18859923, Length=487, Percent_Identity=39.6303901437372, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24585213, Length=551, Percent_Identity=37.0235934664247, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24651556, Length=476, Percent_Identity=41.3865546218487, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI21355981, Length=472, Percent_Identity=38.5593220338983, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24657842, Length=512, Percent_Identity=37.6953125, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24657827, Length=498, Percent_Identity=39.1566265060241, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24645593, Length=363, Percent_Identity=25.6198347107438, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 59947; Mature: 59816

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMRYRLPALAALTTLCVAACASTAGSTTAAPASVKVDVPPVTHPAGETPQWWYRSGAAR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC
AAGNGAMAGKARNVVLFLGDGMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKT
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHCCCHHHHHC
YNTDSQTPDSAGTMTAITTGVKSHMGAIGVSSGNRSDCADSLGKGLLSWLQLADSAGMAT
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GVVTTTRLTHATPAATYAHSPDRNWENDTDLPEAARIAGCQDIAQQLLSTARFGRGPQVV
CEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHCCCCCEEE
LGGGRSQFQTVQERDPEYDDKVGLRLDGRDLVAEWKQSHPQGAYVWNEQQLQAAAGAPAL
ECCCHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEECHHHHHHHCCCCCE
LGLFEPDHMQFDHDRNRTPQGEPSLTEMTRTAIQSLSRDPNGFVLMVEGGRIDHANHAGN
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
AYRALDETVSLSDAVRTAVQTAPPDTLIIVTADHSHTLNFVGYPVRGNPILGKVRGTGGE
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCC
EGDRTQLATDLAGQPYTTLSYANGPGHTGATNAQPAGPKKYPHEPSSSEPASGRPDLTHV
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
DTEAPSYMQESLVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRAR
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHCCEEEEEEEECCHHHHHH
LCKAGTCDSAGVPVNLPAPTTFERATQR
HHCCCCCCCCCCEEECCCCCHHHHHCCC
>Mature Secondary Structure 
PMRYRLPALAALTTLCVAACASTAGSTTAAPASVKVDVPPVTHPAGETPQWWYRSGAAR
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC
AAGNGAMAGKARNVVLFLGDGMSLTTVAAARILDGQRKGGPGEENLLSWEQFPATAFSKT
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHCCCHHHHHC
YNTDSQTPDSAGTMTAITTGVKSHMGAIGVSSGNRSDCADSLGKGLLSWLQLADSAGMAT
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GVVTTTRLTHATPAATYAHSPDRNWENDTDLPEAARIAGCQDIAQQLLSTARFGRGPQVV
CEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHCCCCCEEE
LGGGRSQFQTVQERDPEYDDKVGLRLDGRDLVAEWKQSHPQGAYVWNEQQLQAAAGAPAL
ECCCHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCEEECHHHHHHHCCCCCE
LGLFEPDHMQFDHDRNRTPQGEPSLTEMTRTAIQSLSRDPNGFVLMVEGGRIDHANHAGN
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
AYRALDETVSLSDAVRTAVQTAPPDTLIIVTADHSHTLNFVGYPVRGNPILGKVRGTGGE
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCC
EGDRTQLATDLAGQPYTTLSYANGPGHTGATNAQPAGPKKYPHEPSSSEPASGRPDLTHV
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
DTEAPSYMQESLVPTKSESHGGEDVGIWATGPGSAAFRGTLEENVIYHVIVQATPRLRAR
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHCCEEEEEEEECCHHHHHH
LCKAGTCDSAGVPVNLPAPTTFERATQR
HHCCCCCCCCCCEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 8454193 [H]