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The map label for this gene is tilS

Identifier: 66767844

GI number: 66767844

Start: 1826262

End: 1827569

Strand: Reverse

Name: tilS

Synonym: XC_1518

Alternate gene names: 66767844

Gene position: 1827569-1826262 (Counterclockwise)

Preceding gene: 66767845

Following gene: 66767843

Centisome position: 35.5

GC content: 71.02

Gene sequence:

>1308_bases
GTGGACGTGATGCAGGCAGCCGGGTTGATCCCCGCCACCCCACCGGCACCGGTGCTGGTCGCCTACAGCGGTGGCATGGA
TTCGGCCGTGTTGCTGCACGCCCTGGCCGCCACCGACGGCTACCGCGAGGCCGGCCTGCGCGCCGTGCATGTCCACCATG
GCCTGCATGCGGATGCTGACGCCTGGGCGCTGCACTGCCAACAGCAGTGCGCGGACCTGGGGATTGCCCTGCAGGTCGTG
CGCGTACAGGTGGCACGCGACAGCGGCAGCGGGCTGGAAGCGGCTGCGCGCGCTGCGCGGCATGCGGCCTTTGCAGCGAC
CCTGGCCGCGGGTGAGTGGCTGGCATTGGCGCATCACCGTGACGACCAGGCCGAAACCTTCCTGCTGCGCGCGCTGCGCG
CTTCCGGCCCTGACGGACTGGCGGCGATGCGTCCGCAGCGCCCGTTCGGCCCGGGCACGCTGTGGCGCCCGTTGCTCGGC
CACGCACGCGCCGACCTGGCGGCCTATGCGCAGGCGCATGCAGTGCGCTGGATCGACGACCCCAGCAATACCGATCCGCA
GCACGAGCGCAATTTCCTGCGCACGCAGGTTCTGCCGGCGCTGCAGCAGCGCTGGCCGCTGGCGGCCGATGCACTGGCCC
GCAGCGCGCAGCTGTGCGGCGATGCCAGCACCCTGCTGCACGACGACGACGTCAACCTGCTGCCCACCGTCTGCAACAGC
GCCGGCGCCCTGGAGCTGCAGCCATTGCGCAGCCATTCCCCCGCGCGCCGCGCACGCCTGTTGCGCGCCTGGGTGGCCGC
TGCGCACGCGCCGCCGCTGCCGGCGCAGGGCGTGCACGCACTCGAACGCGAGATCGCCACCACGCAGCCGGATCGGCAGA
CCTGCTTTGCCTGGCAACAGACAGAAATTCGCCGCTGGCGTCAGCAGCTGTACCTGCTCAGTGCGCGCGCGACGTGGCCA
GCGCAGTGGCAGGCCGAGTGGAACGGCGACAGCACACTGTTATTGCCCGACGGCGCGCAACTGCAACTGCGCGGCACGCC
GGGCTTGCGCTTCGAGCAGCCACTGCAAGTGCGCGCGCGCCAGGGGGGCGAGCGCATCGTGCTGCCGGGCCGGACCCATT
CGCATCAGCTCAAACATCTGCTGCAGCAATCAGACCTGCCGCCGTGGGAACGCACGCGCCTGCCCTTGTTGTGGGCCGGC
AAGACGTTGCTGGCCGCCGGTGATCAGATCGTGTCTGCCAAGCTGGACCAGTGGTTGCGTGCGCACGCCGCGCGCCTGCA
GTGGCGCAGCGCTGCACCCGCGAATTGA

Upstream 100 bases:

>100_bases
CTTCGAGCGCGCGACCCAGCGCTGATGGCCGAGCGCACCGCGCACGACCGGTGCGGTGCGCGGCTGGCAGCGGTCGGCGT
CATGACGGGCGTGGCCTGAC

Downstream 100 bases:

>100_bases
CCCGCCTGCGCGCGCGCCGCACACTTTGTCGATGGCCAAGAAGTCTTTGAACGAATCCTCCCCCGTCGCCCGCTTCGAAC
AGTCGCTGGAAGAACTCGAG

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MDVMQAAGLIPATPPAPVLVAYSGGMDSAVLLHALAATDGYREAGLRAVHVHHGLHADADAWALHCQQQCADLGIALQVV
RVQVARDSGSGLEAAARAARHAAFAATLAAGEWLALAHHRDDQAETFLLRALRASGPDGLAAMRPQRPFGPGTLWRPLLG
HARADLAAYAQAHAVRWIDDPSNTDPQHERNFLRTQVLPALQQRWPLAADALARSAQLCGDASTLLHDDDVNLLPTVCNS
AGALELQPLRSHSPARRARLLRAWVAAAHAPPLPAQGVHALEREIATTQPDRQTCFAWQQTEIRRWRQQLYLLSARATWP
AQWQAEWNGDSTLLLPDGAQLQLRGTPGLRFEQPLQVRARQGGERIVLPGRTHSHQLKHLLQQSDLPPWERTRLPLLWAG
KTLLAAGDQIVSAKLDQWLRAHAARLQWRSAAPAN

Sequences:

>Translated_435_residues
MDVMQAAGLIPATPPAPVLVAYSGGMDSAVLLHALAATDGYREAGLRAVHVHHGLHADADAWALHCQQQCADLGIALQVV
RVQVARDSGSGLEAAARAARHAAFAATLAAGEWLALAHHRDDQAETFLLRALRASGPDGLAAMRPQRPFGPGTLWRPLLG
HARADLAAYAQAHAVRWIDDPSNTDPQHERNFLRTQVLPALQQRWPLAADALARSAQLCGDASTLLHDDDVNLLPTVCNS
AGALELQPLRSHSPARRARLLRAWVAAAHAPPLPAQGVHALEREIATTQPDRQTCFAWQQTEIRRWRQQLYLLSARATWP
AQWQAEWNGDSTLLLPDGAQLQLRGTPGLRFEQPLQVRARQGGERIVLPGRTHSHQLKHLLQQSDLPPWERTRLPLLWAG
KTLLAAGDQIVSAKLDQWLRAHAARLQWRSAAPAN
>Mature_435_residues
MDVMQAAGLIPATPPAPVLVAYSGGMDSAVLLHALAATDGYREAGLRAVHVHHGLHADADAWALHCQQQCADLGIALQVV
RVQVARDSGSGLEAAARAARHAAFAATLAAGEWLALAHHRDDQAETFLLRALRASGPDGLAAMRPQRPFGPGTLWRPLLG
HARADLAAYAQAHAVRWIDDPSNTDPQHERNFLRTQVLPALQQRWPLAADALARSAQLCGDASTLLHDDDVNLLPTVCNS
AGALELQPLRSHSPARRARLLRAWVAAAHAPPLPAQGVHALEREIATTQPDRQTCFAWQQTEIRRWRQQLYLLSARATWP
AQWQAEWNGDSTLLLPDGAQLQLRGTPGLRFEQPLQVRARQGGERIVLPGRTHSHQLKHLLQQSDLPPWERTRLPLLWAG
KTLLAAGDQIVSAKLDQWLRAHAARLQWRSAAPAN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=401, Percent_Identity=40.6483790523691, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_XANC8 (Q4UWI7)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_242606.1
- HSSP:   P52097
- ProteinModelPortal:   Q4UWI7
- SMR:   Q4UWI7
- STRING:   Q4UWI7
- GeneID:   3381861
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_1518
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   KSAVGFF
- PhylomeDB:   Q4UWI7
- ProtClustDB:   CLSK446060
- BioCyc:   XCAM314565:XC_1518-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 47552; Mature: 47552

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVMQAAGLIPATPPAPVLVAYSGGMDSAVLLHALAATDGYREAGLRAVHVHHGLHADAD
CCCHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHCCEEEEEECCCCCCCH
AWALHCQQQCADLGIALQVVRVQVARDSGSGLEAAARAARHAAFAATLAAGEWLALAHHR
HHHHHHHHHHHHHHHHHEEHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCC
DDQAETFLLRALRASGPDGLAAMRPQRPFGPGTLWRPLLGHARADLAAYAQAHAVRWIDD
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECC
PSNTDPQHERNFLRTQVLPALQQRWPLAADALARSAQLCGDASTLLHDDDVNLLPTVCNS
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHEECCCCCHHHHHCCC
AGALELQPLRSHSPARRARLLRAWVAAAHAPPLPAQGVHALEREIATTQPDRQTCFAWQQ
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TEIRRWRQQLYLLSARATWPAQWQAEWNGDSTLLLPDGAQLQLRGTPGLRFEQPLQVRAR
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCCCHHHHHH
QGGERIVLPGRTHSHQLKHLLQQSDLPPWERTRLPLLWAGKTLLAAGDQIVSAKLDQWLR
CCCCEEEECCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCHHHHCCHHHHHHHHHHHHH
AHAARLQWRSAAPAN
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MDVMQAAGLIPATPPAPVLVAYSGGMDSAVLLHALAATDGYREAGLRAVHVHHGLHADAD
CCCHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHCCEEEEEECCCCCCCH
AWALHCQQQCADLGIALQVVRVQVARDSGSGLEAAARAARHAAFAATLAAGEWLALAHHR
HHHHHHHHHHHHHHHHHEEHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCC
DDQAETFLLRALRASGPDGLAAMRPQRPFGPGTLWRPLLGHARADLAAYAQAHAVRWIDD
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECC
PSNTDPQHERNFLRTQVLPALQQRWPLAADALARSAQLCGDASTLLHDDDVNLLPTVCNS
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHEECCCCCHHHHHCCC
AGALELQPLRSHSPARRARLLRAWVAAAHAPPLPAQGVHALEREIATTQPDRQTCFAWQQ
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TEIRRWRQQLYLLSARATWPAQWQAEWNGDSTLLLPDGAQLQLRGTPGLRFEQPLQVRAR
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCCCHHHHHH
QGGERIVLPGRTHSHQLKHLLQQSDLPPWERTRLPLLWAGKTLLAAGDQIVSAKLDQWLR
CCCCEEEECCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCHHHHCCHHHHHHHHHHHHH
AHAARLQWRSAAPAN
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA