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The map label for this gene is mexA [H]

Identifier: 66767758

GI number: 66767758

Start: 1727200

End: 1728399

Strand: Direct

Name: mexA [H]

Synonym: XC_1432

Alternate gene names: 66767758

Gene position: 1727200-1728399 (Clockwise)

Preceding gene: 66767756

Following gene: 66767759

Centisome position: 33.55

GC content: 70.67

Gene sequence:

>1200_bases
GTGCGTCCTGTCCCCTCGCTTCGCCTGCCGCTCCTCGCCGTTGCCGCCTCCGTCCTGATCAGCGCCTGCGGTAGCCCGCC
GGGCGGGCCGCCCCCGCAGCAGGGCACGCCGGTGGTCGGCGTCATCACCGTCCGGCCCCAGCCGGTCACCCTGACCACCC
AGTTGCCCGGCCGCACCGTGCCGTACCTGATCGCCGAAGTACGGCCCCAGGTGGGCGGCATCGTGCAGACGCGGCAGTTC
ACCGAGGGCGGCGACGTGAAGGCAGGGCAGACCCTGTACCAGATCGACCCGGCCACCTACCGCGCCAGCTACGCCAGCGC
CCAGGCCACCCTGGCCAAGGCGCAGGCCAACCTGCGCACCGCACGGCTCAAGGCCGACCGCTACAAGGAACTGGTGCAGA
TCAAGGCGATCAGCCAGCAGGAGGGCGACGACACCGCCGCCACGCTCGGCCAGGCCGAAGCGGATGTGGCGGCCGGCAAG
GCCAGCGTGGAGACCGCGCGCATCAATCTGGCGTTCGCCCGCATGGATGCGCCGATCTCCGGCCGGATCGGCCGCTCCAG
CGTGACGCCCGGCGCGCTGGTCACCGCCAACCAGGCCACGGCATTGACCACCATCCAGCAACTGGATCCGATCTACGTGG
ACGTGACCCAGCCCAGCGCCGCCGTGCTGCGGCTGAAGAAGGCGATGGCCCGTGGCGATCTGGCGCGTGCCGGCGACGGT
GCCGCGCAGGTGAGCCTGGTGCTGGAAGACGGCAGCACCTATCCGCTGCAGGGCCGGCTGGCGTTCTCCGATGTGACCGT
GGACCAGAACACCGGCTCGATCAGCCTGCGTGCGGTGTTCCCCAACCCGGATGCCGATCTGCTGCCGGGCATGTACGTGC
GCGCGGTGCTGCAGGAAGGCGTCAAGGCGCAGGGTGTGCTGGTGCCGCAACAGGCGGTGACGCGCAATGGCGCCGGCAAG
CCGACCGCCTTCGTAGTCGGTGCCGACAATAAATTGCAGCTGCGCGTGCTGGAGACCGACCGTGCGGTGGGTGACCAGTG
GCTGGTGCGCAGTGGCCTGAAGACCGGTGACCAACTGGTGGTGGATGGCCTGTCGCGCGCCCGCGACGGTGTCCAGGTCA
AGACCGTGCCGTGGCAACCGACGTCGGCGTCCGTTGCCGGCAGCCCCTCCGCCCCAGCCGCACCGCGTGCGGCCAACTAA

Upstream 100 bases:

>100_bases
CAACTGGGCAGAAATGCTTGACGCCCACGGATGAGCTTATTAGGATACCGAACCGTACCGTTTAGATTTATCCATCGCGG
TTTTCCCGGAGACCTTCCCC

Downstream 100 bases:

>100_bases
GGCGGACTCCAGCTCATGGCACGCTTCTTCATCGACCGCCCGATCTTCGCCTGGGTGCTGGCCATCATCGTGATGCTGGC
CGGCATCCTGTCCATCGCCA

Product: multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRPVPSLRLPLLAVAASVLISACGSPPGGPPPQQGTPVVGVITVRPQPVTLTTQLPGRTVPYLIAEVRPQVGGIVQTRQF
TEGGDVKAGQTLYQIDPATYRASYASAQATLAKAQANLRTARLKADRYKELVQIKAISQQEGDDTAATLGQAEADVAAGK
ASVETARINLAFARMDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYVDVTQPSAAVLRLKKAMARGDLARAGDG
AAQVSLVLEDGSTYPLQGRLAFSDVTVDQNTGSISLRAVFPNPDADLLPGMYVRAVLQEGVKAQGVLVPQQAVTRNGAGK
PTAFVVGADNKLQLRVLETDRAVGDQWLVRSGLKTGDQLVVDGLSRARDGVQVKTVPWQPTSASVAGSPSAPAAPRAAN

Sequences:

>Translated_399_residues
MRPVPSLRLPLLAVAASVLISACGSPPGGPPPQQGTPVVGVITVRPQPVTLTTQLPGRTVPYLIAEVRPQVGGIVQTRQF
TEGGDVKAGQTLYQIDPATYRASYASAQATLAKAQANLRTARLKADRYKELVQIKAISQQEGDDTAATLGQAEADVAAGK
ASVETARINLAFARMDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYVDVTQPSAAVLRLKKAMARGDLARAGDG
AAQVSLVLEDGSTYPLQGRLAFSDVTVDQNTGSISLRAVFPNPDADLLPGMYVRAVLQEGVKAQGVLVPQQAVTRNGAGK
PTAFVVGADNKLQLRVLETDRAVGDQWLVRSGLKTGDQLVVDGLSRARDGVQVKTVPWQPTSASVAGSPSAPAAPRAAN
>Mature_399_residues
MRPVPSLRLPLLAVAASVLISACGSPPGGPPPQQGTPVVGVITVRPQPVTLTTQLPGRTVPYLIAEVRPQVGGIVQTRQF
TEGGDVKAGQTLYQIDPATYRASYASAQATLAKAQANLRTARLKADRYKELVQIKAISQQEGDDTAATLGQAEADVAAGK
ASVETARINLAFARMDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYVDVTQPSAAVLRLKKAMARGDLARAGDG
AAQVSLVLEDGSTYPLQGRLAFSDVTVDQNTGSISLRAVFPNPDADLLPGMYVRAVLQEGVKAQGVLVPQQAVTRNGAGK
PTAFVVGADNKLQLRVLETDRAVGDQWLVRSGLKTGDQLVVDGLSRARDGVQVKTVPWQPTSASVAGSPSAPAAPRAAN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=364, Percent_Identity=55.2197802197802, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789665, Length=366, Percent_Identity=53.2786885245902, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789929, Length=378, Percent_Identity=47.0899470899471, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI87082036, Length=332, Percent_Identity=29.8192771084337, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI87081796, Length=206, Percent_Identity=29.6116504854369, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41512; Mature: 41512

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVPSLRLPLLAVAASVLISACGSPPGGPPPQQGTPVVGVITVRPQPVTLTTQLPGRTV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCH
PYLIAEVRPQVGGIVQTRQFTEGGDVKAGQTLYQIDPATYRASYASAQATLAKAQANLRT
HHHHHHHCHHHCCEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ARLKADRYKELVQIKAISQQEGDDTAATLGQAEADVAAGKASVETARINLAFARMDAPIS
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHCCCCEEEEEEEEEEEEEECCCCC
GRIGRSSVTPGALVTANQATALTTIQQLDPIYVDVTQPSAAVLRLKKAMARGDLARAGDG
CCCCCCCCCCCEEEECCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCC
AAQVSLVLEDGSTYPLQGRLAFSDVTVDQNTGSISLRAVFPNPDADLLPGMYVRAVLQEG
CEEEEEEEECCCCCCEECEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
VKAQGVLVPQQAVTRNGAGKPTAFVVGADNKLQLRVLETDRAVGDQWLVRSGLKTGDQLV
CCCCCEEECHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VDGLSRARDGVQVKTVPWQPTSASVAGSPSAPAAPRAAN
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPVPSLRLPLLAVAASVLISACGSPPGGPPPQQGTPVVGVITVRPQPVTLTTQLPGRTV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCH
PYLIAEVRPQVGGIVQTRQFTEGGDVKAGQTLYQIDPATYRASYASAQATLAKAQANLRT
HHHHHHHCHHHCCEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ARLKADRYKELVQIKAISQQEGDDTAATLGQAEADVAAGKASVETARINLAFARMDAPIS
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHCCCCEEEEEEEEEEEEEECCCCC
GRIGRSSVTPGALVTANQATALTTIQQLDPIYVDVTQPSAAVLRLKKAMARGDLARAGDG
CCCCCCCCCCCEEEECCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCC
AAQVSLVLEDGSTYPLQGRLAFSDVTVDQNTGSISLRAVFPNPDADLLPGMYVRAVLQEG
CEEEEEEEECCCCCCEECEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
VKAQGVLVPQQAVTRNGAGKPTAFVVGADNKLQLRVLETDRAVGDQWLVRSGLKTGDQLV
CCCCCEEECHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VDGLSRARDGVQVKTVPWQPTSASVAGSPSAPAAPRAAN
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]