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The map label for this gene is prfC [H]

Identifier: 66767576

GI number: 66767576

Start: 1528289

End: 1529893

Strand: Reverse

Name: prfC [H]

Synonym: XC_1249

Alternate gene names: 66767576

Gene position: 1529893-1528289 (Counterclockwise)

Preceding gene: 77761142

Following gene: 66767575

Centisome position: 29.71

GC content: 63.99

Gene sequence:

>1605_bases
ATGTCCGAAGTCTCCAACGAAGCCGCGCGCCGCCGCACCTTCGCCATTATTTCCCATCCAGACGCCGGCAAGACCACGCT
GACCGAAAAGCTGCTGCTGTTCGGCGGCGCGATCCAGATGGCCGGCTCGGTGAAGGGACGCAAGGCTGCCCGCCACGCCA
CCTCGGACTGGATGGCGCTGGAAAAAGAGCGCGGCATCTCGGTGACCTCCTCGGTGATGCAGTTCCCGTACGAGGACAAG
ATCGTCAACCTGCTCGACACCCCCGGCCACGCCGACTTCGGCGAGGACACCTATCGCGTGCTGACCGCGGTGGACTCGGC
GCTGATGGTGATCGACGTGGCCAAGGGCGTGGAAGAACGCACCATCAAGTTGATGGAAGTCTGCCGCCTGCGCGACACCC
CCATCATGACCTTCATCAACAAGCTCGATCGCGAAGGCAAGAACCCGATCGACCTGCTGGACGAAGTGGAAACGGTGCTG
GGCATCCAGTGCGCCCCGGTCACCTGGCCGATCGGCATGGGCCAGCGCCTGAAGGGTGTGGTGCATCTGATCACCGGCGA
GGTGCACCTGTACGAGCAGGGCCGCAACTTCACCCGTCAGGACTCCACCATTTTTCCCTCGCTCGATGCACCGGGTCTTG
CCGAAAAGATTGGCACGCAGATGCTGGACGAACTGCGCGAGGAGCTGGAACTGGTGCAAGGCGCCAGCAATCCGTTCGAC
CTGGATGCCTACCGCGCCGGGCAGCAGACGCCGGTGTTCTTCGGCTCGGGCGTCAACAACTTCGGCGTGCAGCCGTTGCT
GGATTTCTTCGTCGAGCACGCACCGCCGCCGCAGGCACGCGATACCACCGGCCGCCGGGTCGAAGCGGTCGAGCCCAAGT
TGAGCGGCTTCGTGTTCAAGATCCAGGCCAACATGGACCCGCAGCACCGCGACCGCGTGGCGTTCATGCGCGTGTGCTCG
GGCAAGTTCACCGCCGGCATGAAGGCATTGCACGTGCGCAGCGGCAAGGACGTGAAGCTGGCCAACGCGCTGACCTTCAT
GGCCTCCGATCGCGAGATCGCCGCCGAAGCGTGGCCGGGCGATGTGATCGGCATCCACAACCACGGCACCATCTCCATCG
GCGATACCTTCACCGAAGGCGAATCGCTGTCGTTCACCGGCATTCCCAACTTCGCGCCGGAATTGTTCCGCCGCGCACGC
CTGCGCGACCCGCTCAAGCTCAAGCAGTTGCAGAAGGGCCTGGCACAATTGTCCGAAGAAGGCGCCACGCAGTTCTTCCG
CCCGCTGATGAGCAACGACTTGATCCTCGGCGCGGTCGGTGTGCTGCAGTTCGACGTGGTGGCCTACCGGCTCAAGGACG
AGTACGGCGTGGATGCGATCTTCGAACCGGTGAGCGTCACCACCGCACGCTGGGTGCACTGCGACAACCCCAAGAAGTTG
GAAGAATTCCGCGAGAAGAACGCCGGCAATCTGGGCATCGATGCGGCCGGGCAACTGGTGTATCTCGCACCCACCCGGGT
CAACCTGCAACTGGCCCAGGAACGCGCGCCGGACGTGCGCTTTTCCGCAACGCGCGAACACGCCTACGCCACGGCGGTCG
ATTGA

Upstream 100 bases:

>100_bases
CCAGCCAGGCGCCCTGGGCTACCGCTGGCCGGCGCACCGAAGCCGCTATAATCACCGGCTTCCTCAGTTTCCACAGGCGT
CCGACCGGGCGCGCCCCCTC

Downstream 100 bases:

>100_bases
CCGCCACTTGAATCGCCACAAGCCAGCGCCACCGCTGGCGATGCGATTTGCGCGCGGTTACGGTACCTTTTGGGCATGAA
TGCAGATGCCCCCCCCTCCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMALEKERGISVTSSVMQFPYEDK
IVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVL
GIQCAPVTWPIGMGQRLKGVVHLITGEVHLYEQGRNFTRQDSTIFPSLDAPGLAEKIGTQMLDELREELELVQGASNPFD
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQARDTTGRRVEAVEPKLSGFVFKIQANMDPQHRDRVAFMRVCS
GKFTAGMKALHVRSGKDVKLANALTFMASDREIAAEAWPGDVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRAR
LRDPLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
EEFREKNAGNLGIDAAGQLVYLAPTRVNLQLAQERAPDVRFSATREHAYATAVD

Sequences:

>Translated_534_residues
MSEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMALEKERGISVTSSVMQFPYEDK
IVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVL
GIQCAPVTWPIGMGQRLKGVVHLITGEVHLYEQGRNFTRQDSTIFPSLDAPGLAEKIGTQMLDELREELELVQGASNPFD
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQARDTTGRRVEAVEPKLSGFVFKIQANMDPQHRDRVAFMRVCS
GKFTAGMKALHVRSGKDVKLANALTFMASDREIAAEAWPGDVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRAR
LRDPLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
EEFREKNAGNLGIDAAGQLVYLAPTRVNLQLAQERAPDVRFSATREHAYATAVD
>Mature_533_residues
SEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMALEKERGISVTSSVMQFPYEDKI
VNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVLG
IQCAPVTWPIGMGQRLKGVVHLITGEVHLYEQGRNFTRQDSTIFPSLDAPGLAEKIGTQMLDELREELELVQGASNPFDL
DAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQARDTTGRRVEAVEPKLSGFVFKIQANMDPQHRDRVAFMRVCSG
KFTAGMKALHVRSGKDVKLANALTFMASDREIAAEAWPGDVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRARL
RDPLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKLE
EFREKNAGNLGIDAAGQLVYLAPTRVNLQLAQERAPDVRFSATREHAYATAVD

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=28.0412371134021, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI19923640, Length=511, Percent_Identity=25.6360078277886, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI25306283, Length=365, Percent_Identity=27.1232876712329, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=484, Percent_Identity=25.8264462809917, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=29.7297297297297, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=29.746835443038, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=64.326375711575, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=28.2828282828283, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=37.9310344827586, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=521, Percent_Identity=27.447216890595, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=164, Percent_Identity=35.9756097560976, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=149, Percent_Identity=31.5436241610738, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=481, Percent_Identity=30.3534303534304, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6322359, Length=424, Percent_Identity=26.8867924528302, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=28.2608695652174, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582462, Length=489, Percent_Identity=28.0163599182004, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI221458488, Length=514, Percent_Identity=26.4591439688716, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=32.484076433121, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=33.1168831168831, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=33.1168831168831, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=33.1168831168831, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58984; Mature: 58853

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMAL
CCCCHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHH
EKERGISVTSSVMQFPYEDKIVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEER
HHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
TIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVLGIQCAPVTWPIGMGQRLKGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
VHLITGEVHLYEQGRNFTRQDSTIFPSLDAPGLAEKIGTQMLDELREELELVQGASNPFD
EEEEECCEEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQARDTTGRRVEAVEPKLSGFVFK
CHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEE
IQANMDPQHRDRVAFMRVCSGKFTAGMKALHVRSGKDVKLANALTFMASDREIAAEAWPG
EECCCCHHHHHHHHHHHHHCCCHHCCCEEEEECCCCCEEHHHHEEEEECCCHHHHHCCCC
DVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRARLRDPLKLKQLQKGLAQLSEE
CEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHEECCCCCCCCHHCCCCEECEEEEEECCCHHHH
EEFREKNAGNLGIDAAGQLVYLAPTRVNLQLAQERAPDVRFSATREHAYATAVD
HHHHHCCCCCCCCCCCCCEEEEECCEEEEEEHHHCCCCCEEECCCCCCEEECCC
>Mature Secondary Structure 
SEVSNEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSVKGRKAARHATSDWMAL
CCCHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHH
EKERGISVTSSVMQFPYEDKIVNLLDTPGHADFGEDTYRVLTAVDSALMVIDVAKGVEER
HHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
TIKLMEVCRLRDTPIMTFINKLDREGKNPIDLLDEVETVLGIQCAPVTWPIGMGQRLKGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
VHLITGEVHLYEQGRNFTRQDSTIFPSLDAPGLAEKIGTQMLDELREELELVQGASNPFD
EEEEECCEEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDAYRAGQQTPVFFGSGVNNFGVQPLLDFFVEHAPPPQARDTTGRRVEAVEPKLSGFVFK
CHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEE
IQANMDPQHRDRVAFMRVCSGKFTAGMKALHVRSGKDVKLANALTFMASDREIAAEAWPG
EECCCCHHHHHHHHHHHHHCCCHHCCCEEEEECCCCCEEHHHHEEEEECCCHHHHHCCCC
DVIGIHNHGTISIGDTFTEGESLSFTGIPNFAPELFRRARLRDPLKLKQLQKGLAQLSEE
CEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GATQFFRPLMSNDLILGAVGVLQFDVVAYRLKDEYGVDAIFEPVSVTTARWVHCDNPKKL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHEECCCCCCCCHHCCCCEECEEEEEECCCHHHH
EEFREKNAGNLGIDAAGQLVYLAPTRVNLQLAQERAPDVRFSATREHAYATAVD
HHHHHCCCCCCCCCCCCCEEEEECCEEEEEEHHHCCCCCEEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA