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The map label for this gene is hsdM [H]

Identifier: 66767534

GI number: 66767534

Start: 1466423

End: 1467931

Strand: Reverse

Name: hsdM [H]

Synonym: XC_1207

Alternate gene names: 66767534

Gene position: 1467931-1466423 (Counterclockwise)

Preceding gene: 66767535

Following gene: 66767533

Centisome position: 28.51

GC content: 61.76

Gene sequence:

>1509_bases
ATGAACAAGACAGATATCGCCCAGGACACCATCAACGCCGCCGTCTGGACTGCCTGCGACACCTTCCGCGGCACCGTGGA
CCCCAGCGTCTACAAGGACTACGTGCTGACCATGCTGTTCCTGAAATACGTCTCCGACGTCTGGCAGGACCATTACGACG
ACTACAAAACCAAGCACGGCAACAAGCCCGGCCTGATCGAAGAGCTGCTCAAGAATGAGCGTTTCGTACTGCCGCATCGC
GCCAACTTCTACACCCTGTACGACCAGCGCCACCGCCCCGGCAACGGCGAGCGCATCGACACCGCGCTGCACGCCATCGA
AGACGCCAACCTGGGCAAGCTACGCGACGTGTTTCAGGACATCAGCTTCAACGCCAACAAACTCGGCGAGGAGCAGCAGA
AGAACGACCTGCTGCGGCACCTGCTGGAAGACTTCGCCAAGCCCGCGCTCAACCTGCGCCCCTCACGCATTGGGCAGCTC
GACATCATCGGCAATGCCTACGAGTACCTGATCAAGAATTTTGCCTCCAGCAGCGGCAAGAAGGCCGGCGAGTTCTATAC
CCCGCCGGAAGTCTCCGCACTGATGGCGCGTCTGATGGATCCGCAGCAAGGCGACGAGATCTGCGACCCCACCTGCGGCT
CCGGCTCGCTGTTGCTCAAGTGCGGCCGGCTGATCCGCGAGCGCACCGGCAGCGGCAAGTACGCGCTCTACGGCCAGGAG
GCGATCGGCAGCACCTGGGCGCTGGCCAAGATGAACATGTTCCTGCATGGCGAAGACAACCACCGCATCGAATGGGGCGA
CACCATTCGCAATCCCAAGCTGCTGGCCGGCAACCATCTCAAGCACTTCGACATCGTGGTGGCCAACCCACCGTTCTCGC
TGGAAAAGTGGGGCCACGACAGCGCCGATACCGACCCGCACGACCGCTTCCGTCGCGGGCTGCCGCCGCGCACCAAGGGC
GACTACGCCTTCATCCTGCACATGATCGCCACCATGAAGCCGCGCACCGGCCGCATGGCCGTGGTGGTGCCGCACGGTGT
GTTGTTCCGTGGCGCCGCCGAAGGCCGCATCCGCCAGAAATTGATCGAAGAGAACCTGCTGGACGTGGTGATCGGCCTGC
CGGAGAAGCTGTTTTACGGCACCGGCATCCCGGCAGCAGTGTTGGTGTTCCGCACCAAGAAGAAAGACAAAAAAGTGCTG
TTCATCGACGCCAGCCGCCAGTACCAGGACGGCAAGAATCAGAACCTGCTGCGCGAGAGCGACCTGCAACGCATCCTGGA
CACGGTGCAGGCGCGCCAGAATGTAGACAAGTACGCCTACCTGGCCAGCCCGGACGAGATCGCCGGCCACGACTACAACC
TCAACATCCCGCGGTATGTGGATACCTTCGAGGAAGAAGCAGAGATCGACCTGATGGCAGTGCGCCGCGAGCGCGAGCAG
TTGAAGGGCGAGCTGGCAACACTCGAAATGCAGATGGCCGCCTATCTCAAGGAGCTTGGCTATGAGTGA

Upstream 100 bases:

>100_bases
AGGCAGAGCGCGCCGCCCTGCACGCACTGATCAACAACCGCACCGCCGAGCTGGCGATCCTCGCCGAGCGCCTTCTCGCC
TAAGACGTTGGAAACCCCCC

Downstream 100 bases:

>100_bases
CGAAAAAAAAGATATCGCCACACATGCTGACGAGGGCTTCCCGGCACACGACAGTGCCGCAGTCCGGCGCACATGGCACC
AAGGCCAATGGTGGTTTGCG

Product: type I restriction enzyme M protein

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNKTDIAQDTINAAVWTACDTFRGTVDPSVYKDYVLTMLFLKYVSDVWQDHYDDYKTKHGNKPGLIEELLKNERFVLPHR
ANFYTLYDQRHRPGNGERIDTALHAIEDANLGKLRDVFQDISFNANKLGEEQQKNDLLRHLLEDFAKPALNLRPSRIGQL
DIIGNAYEYLIKNFASSSGKKAGEFYTPPEVSALMARLMDPQQGDEICDPTCGSGSLLLKCGRLIRERTGSGKYALYGQE
AIGSTWALAKMNMFLHGEDNHRIEWGDTIRNPKLLAGNHLKHFDIVVANPPFSLEKWGHDSADTDPHDRFRRGLPPRTKG
DYAFILHMIATMKPRTGRMAVVVPHGVLFRGAAEGRIRQKLIEENLLDVVIGLPEKLFYGTGIPAAVLVFRTKKKDKKVL
FIDASRQYQDGKNQNLLRESDLQRILDTVQARQNVDKYAYLASPDEIAGHDYNLNIPRYVDTFEEEAEIDLMAVRREREQ
LKGELATLEMQMAAYLKELGYE

Sequences:

>Translated_502_residues
MNKTDIAQDTINAAVWTACDTFRGTVDPSVYKDYVLTMLFLKYVSDVWQDHYDDYKTKHGNKPGLIEELLKNERFVLPHR
ANFYTLYDQRHRPGNGERIDTALHAIEDANLGKLRDVFQDISFNANKLGEEQQKNDLLRHLLEDFAKPALNLRPSRIGQL
DIIGNAYEYLIKNFASSSGKKAGEFYTPPEVSALMARLMDPQQGDEICDPTCGSGSLLLKCGRLIRERTGSGKYALYGQE
AIGSTWALAKMNMFLHGEDNHRIEWGDTIRNPKLLAGNHLKHFDIVVANPPFSLEKWGHDSADTDPHDRFRRGLPPRTKG
DYAFILHMIATMKPRTGRMAVVVPHGVLFRGAAEGRIRQKLIEENLLDVVIGLPEKLFYGTGIPAAVLVFRTKKKDKKVL
FIDASRQYQDGKNQNLLRESDLQRILDTVQARQNVDKYAYLASPDEIAGHDYNLNIPRYVDTFEEEAEIDLMAVRREREQ
LKGELATLEMQMAAYLKELGYE
>Mature_502_residues
MNKTDIAQDTINAAVWTACDTFRGTVDPSVYKDYVLTMLFLKYVSDVWQDHYDDYKTKHGNKPGLIEELLKNERFVLPHR
ANFYTLYDQRHRPGNGERIDTALHAIEDANLGKLRDVFQDISFNANKLGEEQQKNDLLRHLLEDFAKPALNLRPSRIGQL
DIIGNAYEYLIKNFASSSGKKAGEFYTPPEVSALMARLMDPQQGDEICDPTCGSGSLLLKCGRLIRERTGSGKYALYGQE
AIGSTWALAKMNMFLHGEDNHRIEWGDTIRNPKLLAGNHLKHFDIVVANPPFSLEKWGHDSADTDPHDRFRRGLPPRTKG
DYAFILHMIATMKPRTGRMAVVVPHGVLFRGAAEGRIRQKLIEENLLDVVIGLPEKLFYGTGIPAAVLVFRTKKKDKKVL
FIDASRQYQDGKNQNLLRESDLQRILDTVQARQNVDKYAYLASPDEIAGHDYNLNIPRYVDTFEEEAEIDLMAVRREREQ
LKGELATLEMQMAAYLKELGYE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=414, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 57177; Mature: 57177

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTDIAQDTINAAVWTACDTFRGTVDPSVYKDYVLTMLFLKYVSDVWQDHYDDYKTKHG
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NKPGLIEELLKNERFVLPHRANFYTLYDQRHRPGNGERIDTALHAIEDANLGKLRDVFQD
CCCCHHHHHHCCCCEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
ISFNANKLGEEQQKNDLLRHLLEDFAKPALNLRPSRIGQLDIIGNAYEYLIKNFASSSGK
HCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCC
KAGEFYTPPEVSALMARLMDPQQGDEICDPTCGSGSLLLKCGRLIRERTGSGKYALYGQE
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHH
AIGSTWALAKMNMFLHGEDNHRIEWGDTIRNPKLLAGNHLKHFDIVVANPPFSLEKWGHD
HCCCHHHHHHEEEEEECCCCCEEECCCCCCCCCEEECCCCEEEEEEEECCCCCHHHCCCC
SADTDPHDRFRRGLPPRTKGDYAFILHMIATMKPRTGRMAVVVPHGVLFRGAAEGRIRQK
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCHHHHHHH
LIEENLLDVVIGLPEKLFYGTGIPAAVLVFRTKKKDKKVLFIDASRQYQDGKNQNLLRES
HHHHHHHHHHHCCCHHHHHCCCCHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCHHHHH
DLQRILDTVQARQNVDKYAYLASPDEIAGHDYNLNIPRYVDTFEEEAEIDLMAVRREREQ
HHHHHHHHHHHHHCHHHEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKGELATLEMQMAAYLKELGYE
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKTDIAQDTINAAVWTACDTFRGTVDPSVYKDYVLTMLFLKYVSDVWQDHYDDYKTKHG
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NKPGLIEELLKNERFVLPHRANFYTLYDQRHRPGNGERIDTALHAIEDANLGKLRDVFQD
CCCCHHHHHHCCCCEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
ISFNANKLGEEQQKNDLLRHLLEDFAKPALNLRPSRIGQLDIIGNAYEYLIKNFASSSGK
HCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCC
KAGEFYTPPEVSALMARLMDPQQGDEICDPTCGSGSLLLKCGRLIRERTGSGKYALYGQE
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHH
AIGSTWALAKMNMFLHGEDNHRIEWGDTIRNPKLLAGNHLKHFDIVVANPPFSLEKWGHD
HCCCHHHHHHEEEEEECCCCCEEECCCCCCCCCEEECCCCEEEEEEEECCCCCHHHCCCC
SADTDPHDRFRRGLPPRTKGDYAFILHMIATMKPRTGRMAVVVPHGVLFRGAAEGRIRQK
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCHHHHHHH
LIEENLLDVVIGLPEKLFYGTGIPAAVLVFRTKKKDKKVLFIDASRQYQDGKNQNLLRES
HHHHHHHHHHHCCCHHHHHCCCCHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCHHHHH
DLQRILDTVQARQNVDKYAYLASPDEIAGHDYNLNIPRYVDTFEEEAEIDLMAVRREREQ
HHHHHHHHHHHHHCHHHEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKGELATLEMQMAAYLKELGYE
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]