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The map label for this gene is cysI [H]

Identifier: 66767323

GI number: 66767323

Start: 1188882

End: 1190588

Strand: Reverse

Name: cysI [H]

Synonym: XC_0991

Alternate gene names: 66767323

Gene position: 1190588-1188882 (Counterclockwise)

Preceding gene: 66767324

Following gene: 66767322

Centisome position: 23.12

GC content: 66.26

Gene sequence:

>1707_bases
ATGACCCACTCCGTCGAAGACATCAAATCCACCAGCCGCCGCTTGCGCGGGTCGCTGGAGCAGAGCCTGGCCGATCCGGT
CACCGGGGCGTTGCGCGAGGACGATCAGACGCTGATCAAATACCACGGCAGCTACCAGCAGGACGACCGCGACATCCGCG
ATGAGCGGCGGCGGCAAAAGCTCGAGCCGGCATATCAGTTCATGATCCGCACGCGCACGCCAGGTGGAGTGATTTCGCCC
AGCCAGTGGCTGGCACTGGATGGCATCGCCACGCGCTATGCCAACCATTCGCTGCGCATCACCACGCGCCAGGCGTTCCA
GTTCCATGGTGTGATCAAGCGCGAGCTCAAGGCCACGATGCAGGCGATCAACGCCACCTTGATCGACACGCTGGCCGCCT
GCGGCGATGTGAATCGCAATGTGCAGGTGGCGGCCAACCCGCTGCTGTCGCAGGCGCATGCCACGCTGTATGCCGATGCC
GCGCGCGTGTCCGAGCATCTGCTGCCCAACACGCGTGCCTATTACGAGATCTGGCTGGATGAAGAGCGCGTGTCCGGTTC
GGGCGCCGAGGACGAACCGATCTATGGCGACCGCTACCTGCCGCGCAAATTCAAGATCGGCTTTGCCGCGCCACCCCATA
ACGATGTGGACGTGTTCGCCAACGACCTGGGCTTCATCGCCATCCTGCGCGAGGGCCAGTTGCTCGGCTACAACGTCAGC
ATCGGCGGCGGCATGGGCGCCAGCCACGGCGATGCGGAGACCTGGCCGCGCGTGGCCAACGTGATCGGCTTCGTCACCGG
CGATCAGTTGCTGGATATCGCCACGGCCGTGGTCACCACCCAGCGCGACCTCGGCAATCGCGCCGTGCGTAAGCGCGCGC
GCTTCAAGTACACCATCGACGACCACGGCCTGGACACCATCGTGGCGGAGATCGAACGCCGCGCGGGATTCAGCTTGCAA
CCTGCGCAGCCGTTTGCATTCGCGCATAACGGCGACCGCTACGGCTGGGTGGAGGGCGAACAGGGCGATTGGCATCTGAC
GCTGTCGCTGCCGGCCGGGCGCATTGCCGACACCGACACCGCCGCGCACCTGAGCGGGCTGCGCGCCATCGCGCAGTTGC
AGATGGGCGAGTTCCGCATGACGCCCAATCAGAACCTGGTGATTGCCGGCGTCCCGGCCGAACAGCGCGCGCAGATCGAC
CAACTGGTGGCGCAGTACGGCCTGGATGCCGGCAACCTGGCGCCCACCGCGTTGGCGCGCGGCGCGATGGCCTGTGTGGC
CTTGCCAACCTGCGGGCTGGCCATGGCCGAGGCCGAGCGCTATCTGCCGGATTTCAGTGCAGCGCTGCAGCCGCTGCTGG
AGCGTCACGGGCTGGCGCAGACGCCGATCGTGCTGCGCCTGTCCGGTTGCCCGAACGGCTGTTCGCGGCCGTATCTGGCT
GAAATCGCATTGGTCGGCAAGGCACCGGGCCGCTACAACCTCATGCTGGGTGGCGACCGCCGCGGCCAACGCCTGAACAC
GCTGTACCGCGAAAACATCAACGAGGCCGACATCCTCGCCGCGCTCGAGCCACTGCTGGCCCGTTACGCAGCAGAGCGCG
ACCGACACACCGATGAAGGCTTCGGCGACTTCCTGCATCGCAGCGGCCTGATCGCCCTGCCGCCCTACCCCACCCACCGT
CATCTCGACCTGGAATTGCTCGCATGA

Upstream 100 bases:

>100_bases
GTGGGGAATCGGGAATGGGTACGGCCTGCACCCGAATTGTCGCGAGCGACCACGCCGCTTTTCACGATTCCCCTTTCCCG
ATTCCCGATTCCCGATTCCC

Downstream 100 bases:

>100_bases
CCGCGCTGCCTGCCACATCGATCGCCGCTCCTTCGCTGGACGACCTGGACGCGCTCAACGCGCACCTGGAGACGCTGCGC
GCCGACGAACGCGTTGCCTG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MTHSVEDIKSTSRRLRGSLEQSLADPVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQKLEPAYQFMIRTRTPGGVISP
SQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATMQAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADA
ARVSEHLLPNTRAYYEIWLDEERVSGSGAEDEPIYGDRYLPRKFKIGFAAPPHNDVDVFANDLGFIAILREGQLLGYNVS
IGGGMGASHGDAETWPRVANVIGFVTGDQLLDIATAVVTTQRDLGNRAVRKRARFKYTIDDHGLDTIVAEIERRAGFSLQ
PAQPFAFAHNGDRYGWVEGEQGDWHLTLSLPAGRIADTDTAAHLSGLRAIAQLQMGEFRMTPNQNLVIAGVPAEQRAQID
QLVAQYGLDAGNLAPTALARGAMACVALPTCGLAMAEAERYLPDFSAALQPLLERHGLAQTPIVLRLSGCPNGCSRPYLA
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENINEADILAALEPLLARYAAERDRHTDEGFGDFLHRSGLIALPPYPTHR
HLDLELLA

Sequences:

>Translated_568_residues
MTHSVEDIKSTSRRLRGSLEQSLADPVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQKLEPAYQFMIRTRTPGGVISP
SQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATMQAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADA
ARVSEHLLPNTRAYYEIWLDEERVSGSGAEDEPIYGDRYLPRKFKIGFAAPPHNDVDVFANDLGFIAILREGQLLGYNVS
IGGGMGASHGDAETWPRVANVIGFVTGDQLLDIATAVVTTQRDLGNRAVRKRARFKYTIDDHGLDTIVAEIERRAGFSLQ
PAQPFAFAHNGDRYGWVEGEQGDWHLTLSLPAGRIADTDTAAHLSGLRAIAQLQMGEFRMTPNQNLVIAGVPAEQRAQID
QLVAQYGLDAGNLAPTALARGAMACVALPTCGLAMAEAERYLPDFSAALQPLLERHGLAQTPIVLRLSGCPNGCSRPYLA
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENINEADILAALEPLLARYAAERDRHTDEGFGDFLHRSGLIALPPYPTHR
HLDLELLA
>Mature_567_residues
THSVEDIKSTSRRLRGSLEQSLADPVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQKLEPAYQFMIRTRTPGGVISPS
QWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATMQAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADAA
RVSEHLLPNTRAYYEIWLDEERVSGSGAEDEPIYGDRYLPRKFKIGFAAPPHNDVDVFANDLGFIAILREGQLLGYNVSI
GGGMGASHGDAETWPRVANVIGFVTGDQLLDIATAVVTTQRDLGNRAVRKRARFKYTIDDHGLDTIVAEIERRAGFSLQP
AQPFAFAHNGDRYGWVEGEQGDWHLTLSLPAGRIADTDTAAHLSGLRAIAQLQMGEFRMTPNQNLVIAGVPAEQRAQIDQ
LVAQYGLDAGNLAPTALARGAMACVALPTCGLAMAEAERYLPDFSAALQPLLERHGLAQTPIVLRLSGCPNGCSRPYLAE
IALVGKAPGRYNLMLGGDRRGQRLNTLYRENINEADILAALEPLLARYAAERDRHTDEGFGDFLHRSGLIALPPYPTHRH
LDLELLA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=547, Percent_Identity=48.9945155393053, Blast_Score=552, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6322597, Length=578, Percent_Identity=44.8096885813149, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 62522; Mature: 62391

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSVEDIKSTSRRLRGSLEQSLADPVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
LEPAYQFMIRTRTPGGVISPSQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATM
CCHHHHEEEEECCCCCCCCCCHHEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
QAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADAARVSEHLLPNTRAYYEIWLD
HHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
EERVSGSGAEDEPIYGDRYLPRKFKIGFAAPPHNDVDVFANDLGFIAILREGQLLGYNVS
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEECCEEEEEEEE
IGGGMGASHGDAETWPRVANVIGFVTGDQLLDIATAVVTTQRDLGNRAVRKRARFKYTID
ECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DHGLDTIVAEIERRAGFSLQPAQPFAFAHNGDRYGWVEGEQGDWHLTLSLPAGRIADTDT
CCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCCCCCCCCHH
AAHLSGLRAIAQLQMGEFRMTPNQNLVIAGVPAEQRAQIDQLVAQYGLDAGNLAPTALAR
HHHHHHHHHHHHHHHCCEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
GAMACVALPTCGLAMAEAERYLPDFSAALQPLLERHGLAQTPIVLRLSGCPNGCSRPYLA
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEE
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENINEADILAALEPLLARYAAERDRHTDEG
EEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
FGDFLHRSGLIALPPYPTHRHLDLELLA
HHHHHHHCCEEEECCCCCCCCCEEEEEC
>Mature Secondary Structure 
THSVEDIKSTSRRLRGSLEQSLADPVTGALREDDQTLIKYHGSYQQDDRDIRDERRRQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
LEPAYQFMIRTRTPGGVISPSQWLALDGIATRYANHSLRITTRQAFQFHGVIKRELKATM
CCHHHHEEEEECCCCCCCCCCHHEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
QAINATLIDTLAACGDVNRNVQVAANPLLSQAHATLYADAARVSEHLLPNTRAYYEIWLD
HHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
EERVSGSGAEDEPIYGDRYLPRKFKIGFAAPPHNDVDVFANDLGFIAILREGQLLGYNVS
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEECCEEEEEEEE
IGGGMGASHGDAETWPRVANVIGFVTGDQLLDIATAVVTTQRDLGNRAVRKRARFKYTID
ECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DHGLDTIVAEIERRAGFSLQPAQPFAFAHNGDRYGWVEGEQGDWHLTLSLPAGRIADTDT
CCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCCCCCCCCHH
AAHLSGLRAIAQLQMGEFRMTPNQNLVIAGVPAEQRAQIDQLVAQYGLDAGNLAPTALAR
HHHHHHHHHHHHHHHCCEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
GAMACVALPTCGLAMAEAERYLPDFSAALQPLLERHGLAQTPIVLRLSGCPNGCSRPYLA
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEE
EIALVGKAPGRYNLMLGGDRRGQRLNTLYRENINEADILAALEPLLARYAAERDRHTDEG
EEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
FGDFLHRSGLIALPPYPTHRHLDLELLA
HHHHHHHCCEEEECCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]