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The map label for this gene is thiE

Identifier: 66767227

GI number: 66767227

Start: 1080713

End: 1081336

Strand: Reverse

Name: thiE

Synonym: XC_0895

Alternate gene names: 66767227

Gene position: 1081336-1080713 (Counterclockwise)

Preceding gene: 66767229

Following gene: 66767224

Centisome position: 21.0

GC content: 68.59

Gene sequence:

>624_bases
ATGCCGACCCTCCAAAACGCCCGCGGCGTCTACCTGATTACCCCCGACACCCGCGACACCGCGCAGTTGCTTGCGTGCAC
GTTGCCGCTGCTGCCGCACATCACCTGGTTGCAATACCGCAACAAGCAGGCCGATGCAGCACTGCGCCTGGCCCAGGCCA
CCGCACTGCGCGCGGCATGCACCGCGCATGGCGTGCCATTGATCATCAACGACGACGCGGCGCTTGCGCAGCAGGTGGGC
GCGGACGGTGTGCACCTGGGTGAAGACGATGGCGAGGTGGCGGCAGCGCGCGCGTTGCTGGGTGCCTCGGCCATCATTGG
GGTGTCGTGCTACGACGAGATCGAACGCGCGCGCGCAGCGGCGGCGGCCGGTGCGAACTATGTGGCCTTTGGGGCGTTCT
TCCCCACCGCCACCAAGGTGACCACGCGCCGTGCCACGCCTGCATTGCTGCACGAGGCCGCTGCGTTGGGACTGCCACGT
GTGGCGATCGGCGGCATCACGCCTTCGCAGGTGCCCGAGCTGGTGACCGCGGGCGCCGACTTGATTGCGGTGGTCAGCGG
CGTGTATGCGGCGGCGGACCCGGTCGCCGCAGTGCAGGCGTATCGCGCAGGATTTACGCAGTAG

Upstream 100 bases:

>100_bases
CGGTAGGTGGTCGTGGATGTCTCGCTCATCGGATAATCGCGGTCACGCAACGGTGGGGCTGCATTGTCCCATCCCACCCA
CCGCTTTCGGAAGCGCTGCC

Downstream 100 bases:

>100_bases
CGCAACGAGTGTGAAGCGCTGAGATTTTTATCTCAGCGGTGTCATCTGTTTCAAGACAGCAAGATCTCTTTCGCCGCAGT
GCTTGGCTTGAAACGCCAGA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPTLQNARGVYLITPDTRDTAQLLACTLPLLPHITWLQYRNKQADAALRLAQATALRAACTAHGVPLIINDDAALAQQVG
ADGVHLGEDDGEVAAARALLGASAIIGVSCYDEIERARAAAAAGANYVAFGAFFPTATKVTTRRATPALLHEAAALGLPR
VAIGGITPSQVPELVTAGADLIAVVSGVYAAADPVAAVQAYRAGFTQ

Sequences:

>Translated_207_residues
MPTLQNARGVYLITPDTRDTAQLLACTLPLLPHITWLQYRNKQADAALRLAQATALRAACTAHGVPLIINDDAALAQQVG
ADGVHLGEDDGEVAAARALLGASAIIGVSCYDEIERARAAAAAGANYVAFGAFFPTATKVTTRRATPALLHEAAALGLPR
VAIGGITPSQVPELVTAGADLIAVVSGVYAAADPVAAVQAYRAGFTQ
>Mature_206_residues
PTLQNARGVYLITPDTRDTAQLLACTLPLLPHITWLQYRNKQADAALRLAQATALRAACTAHGVPLIINDDAALAQQVGA
DGVHLGEDDGEVAAARALLGASAIIGVSCYDEIERARAAAAAGANYVAFGAFFPTATKVTTRRATPALLHEAAALGLPRV
AIGGITPSQVPELVTAGADLIAVVSGVYAAADPVAAVQAYRAGFTQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=132, Percent_Identity=38.6363636363636, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=189, Percent_Identity=31.2169312169312, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_XANC8 (Q4UYA4)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_241989.1
- HSSP:   P39594
- ProteinModelPortal:   Q4UYA4
- SMR:   Q4UYA4
- STRING:   Q4UYA4
- GeneID:   3382580
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_0895
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   PRK00043
- BioCyc:   XCAM314565:XC_0895-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21236; Mature: 21105

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLQNARGVYLITPDTRDTAQLLACTLPLLPHITWLQYRNKQADAALRLAQATALRAAC
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TAHGVPLIINDDAALAQQVGADGVHLGEDDGEVAAARALLGASAIIGVSCYDEIERARAA
HHCCCCEEECCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAGANYVAFGAFFPTATKVTTRRATPALLHEAAALGLPRVAIGGITPSQVPELVTAGAD
HHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCHH
LIAVVSGVYAAADPVAAVQAYRAGFTQ
HHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PTLQNARGVYLITPDTRDTAQLLACTLPLLPHITWLQYRNKQADAALRLAQATALRAAC
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TAHGVPLIINDDAALAQQVGADGVHLGEDDGEVAAARALLGASAIIGVSCYDEIERARAA
HHCCCCEEECCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAGANYVAFGAFFPTATKVTTRRATPALLHEAAALGLPRVAIGGITPSQVPELVTAGAD
HHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCHH
LIAVVSGVYAAADPVAAVQAYRAGFTQ
HHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA