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The map label for this gene is hemL [H]

Identifier: 66767224

GI number: 66767224

Start: 1075166

End: 1076455

Strand: Reverse

Name: hemL [H]

Synonym: XC_0892

Alternate gene names: 66767224

Gene position: 1076455-1075166 (Counterclockwise)

Preceding gene: 66767227

Following gene: 66767223

Centisome position: 20.91

GC content: 68.45

Gene sequence:

>1290_bases
ATGAACCACTCCCGCTCCCACGCCCTGTTTTCCCAGGCGCAGAACCTGATGCCCGGCGGCGTCAACTCGCCGGTGCGTGC
GTTCAAGTCGGTGGGCGGCGAGCCGTTCTTCGTCGCGCGTGCCGACGGCGCGTACCTGTTCGACGTGGATGGCAACCGCT
ATATCGACTACGTGGGTTCGTGGGGGCCGATGATTGCCGGCCACAACCACCCGGCCGTGCGCGAAGCGGTGGAACGCGCC
ATCCGCGATGGCCTGTCGTTCGGCGCGCCCTGCGAGGCCGAGGTGACCATGGCCGAAACCATCACCCGGCTGGTGCCATC
CTGCGAAATGGTGCGGATGGTCAATTCCGGCACCGAAGCCACGCTGTCGGCGATCCGCCTGGCACGCGGCGCCACCGGGC
GCAACCGCATCGTCAAGTTCGAGGGCTGCTACCACGGCCACGGCGACTCGTTCCTGGTCAAGGCCGGCAGCGGCATGCTG
ACCCTGGGTGTGCCCACCTCGCCAGGCGTGCCGGCAGGCCTGAGCGAGCTCACCGCCACGCTCAGCTTCAATGATTTCGA
AGGCGCCACCGCGTTGTTCGACGAGATCGGCGCCGAAGTGGCGGCGGTGATCATCGAGCCGGTGGTCGGCAATGCCAACT
GCATTCCGCCGCAGGCCGGCTATCTGCAGCACCTGCGCACGCTGTGCACCCGCCACGGCGCGCTGCTGATCTTCGACGAA
GTGATGACCGGCTTCCGCGTGGCGCTGGGCGGCGCGCAGGCGCACTACGGCGTGACCCCGGACCTGACCACCTTCGGCAA
GATCATCGGCGGCGGCATGCCGGTGGGCGCCTACGGCGGCGGCCGCGACCTGATGGAACAGATCTCCCCGGCCGGCCCGA
TCTACCAGGCCGGCACTTTGTCTGGCAACCCGGTGGCAATGGCCGCGGGCCTGGCGATGCTGCAACTGGTGCAGGAACCG
GGCTTCCACGCCCGCCTCAGCGAGACCACCAGCCTGCTGTGCGAAGGCCTGGAAGACGCCGCCCGCGCCGCCGGGGTAGC
GGTGACCACCAACCAGGTGGGCGGCATGTTCGGGCTGTTCTTCACCGACCAGATCGTGGAGAACTACGCCCAGGCCACCG
CCTGCGACGTAACCACCTTCAACCGCTTCTTCCACGCGATGCTGCAGCAGGGCGTGTACCTGGCGCCATCGGCCTACGAG
GCGGGCTTCGTATCCAGCGCCCACGACGAGGCCGTCATCGAGGCCACCCTGGCCGCCGCACGCGAAGCCTTTGCCGACGT
CATGCGCTGA

Upstream 100 bases:

>100_bases
GGTGGGTAGGCGGCGGGCGGCCGAAGCCTGACAATGGCCGGCGCGTCGCCCCGCTCCGGGTGCCGACGCCCCCACCGGCA
CCACTCAAGGATCTGCGCTC

Downstream 100 bases:

>100_bases
GCTCATGCGCGCGGCTGTGACCTGGTCCCACCTTTGCTTTTGTCTTGTGGCTGTCATACCTTGCTGACAGGTGCCAAGGG
ATGGGCATGGGTGGGGACCC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNHSRSHALFSQAQNLMPGGVNSPVRAFKSVGGEPFFVARADGAYLFDVDGNRYIDYVGSWGPMIAGHNHPAVREAVERA
IRDGLSFGAPCEAEVTMAETITRLVPSCEMVRMVNSGTEATLSAIRLARGATGRNRIVKFEGCYHGHGDSFLVKAGSGML
TLGVPTSPGVPAGLSELTATLSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGGRDLMEQISPAGPIYQAGTLSGNPVAMAAGLAMLQLVQEP
GFHARLSETTSLLCEGLEDAARAAGVAVTTNQVGGMFGLFFTDQIVENYAQATACDVTTFNRFFHAMLQQGVYLAPSAYE
AGFVSSAHDEAVIEATLAAAREAFADVMR

Sequences:

>Translated_429_residues
MNHSRSHALFSQAQNLMPGGVNSPVRAFKSVGGEPFFVARADGAYLFDVDGNRYIDYVGSWGPMIAGHNHPAVREAVERA
IRDGLSFGAPCEAEVTMAETITRLVPSCEMVRMVNSGTEATLSAIRLARGATGRNRIVKFEGCYHGHGDSFLVKAGSGML
TLGVPTSPGVPAGLSELTATLSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGGRDLMEQISPAGPIYQAGTLSGNPVAMAAGLAMLQLVQEP
GFHARLSETTSLLCEGLEDAARAAGVAVTTNQVGGMFGLFFTDQIVENYAQATACDVTTFNRFFHAMLQQGVYLAPSAYE
AGFVSSAHDEAVIEATLAAAREAFADVMR
>Mature_429_residues
MNHSRSHALFSQAQNLMPGGVNSPVRAFKSVGGEPFFVARADGAYLFDVDGNRYIDYVGSWGPMIAGHNHPAVREAVERA
IRDGLSFGAPCEAEVTMAETITRLVPSCEMVRMVNSGTEATLSAIRLARGATGRNRIVKFEGCYHGHGDSFLVKAGSGML
TLGVPTSPGVPAGLSELTATLSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGGRDLMEQISPAGPIYQAGTLSGNPVAMAAGLAMLQLVQEP
GFHARLSETTSLLCEGLEDAARAAGVAVTTNQVGGMFGLFFTDQIVENYAQATACDVTTFNRFFHAMLQQGVYLAPSAYE
AGFVSSAHDEAVIEATLAAAREAFADVMR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=30.3030303030303, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI37574042, Length=375, Percent_Identity=25.6, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI226442705, Length=367, Percent_Identity=25.0681198910082, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI284507298, Length=215, Percent_Identity=31.1627906976744, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI226442709, Length=342, Percent_Identity=25.4385964912281, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=63.0588235294118, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1789016, Length=323, Percent_Identity=31.8885448916409, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788044, Length=295, Percent_Identity=34.9152542372881, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1787560, Length=345, Percent_Identity=31.8840579710145, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI145693181, Length=340, Percent_Identity=30, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789759, Length=311, Percent_Identity=28.9389067524116, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786991, Length=383, Percent_Identity=30.0261096605744, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=336, Percent_Identity=27.9761904761905, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=348, Percent_Identity=27.2988505747126, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=344, Percent_Identity=23.8372093023256, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=341, Percent_Identity=28.4457478005865, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=363, Percent_Identity=24.7933884297521, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21357415, Length=293, Percent_Identity=27.6450511945392, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574759, Length=400, Percent_Identity=25.5, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161085790, Length=400, Percent_Identity=25.5, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45062; Mature: 45062

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSRSHALFSQAQNLMPGGVNSPVRAFKSVGGEPFFVARADGAYLFDVDGNRYIDYVGS
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEECCCCEEEEECCC
WGPMIAGHNHPAVREAVERAIRDGLSFGAPCEAEVTMAETITRLVPSCEMVRMVNSGTEA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCHHH
TLSAIRLARGATGRNRIVKFEGCYHGHGDSFLVKAGSGMLTLGVPTSPGVPAGLSELTAT
HHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCHHHHEEE
LSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHH
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGGRDLMEQISPAGPIYQAGTL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEECCCC
SGNPVAMAAGLAMLQLVQEPGFHARLSETTSLLCEGLEDAARAAGVAVTTNQVGGMFGLF
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
FTDQIVENYAQATACDVTTFNRFFHAMLQQGVYLAPSAYEAGFVSSAHDEAVIEATLAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
REAFADVMR
HHHHHHHHC
>Mature Secondary Structure
MNHSRSHALFSQAQNLMPGGVNSPVRAFKSVGGEPFFVARADGAYLFDVDGNRYIDYVGS
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEECCCCEEEEECCC
WGPMIAGHNHPAVREAVERAIRDGLSFGAPCEAEVTMAETITRLVPSCEMVRMVNSGTEA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCHHH
TLSAIRLARGATGRNRIVKFEGCYHGHGDSFLVKAGSGMLTLGVPTSPGVPAGLSELTAT
HHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCHHHHEEE
LSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHH
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGGRDLMEQISPAGPIYQAGTL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEECCCC
SGNPVAMAAGLAMLQLVQEPGFHARLSETTSLLCEGLEDAARAAGVAVTTNQVGGMFGLF
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
FTDQIVENYAQATACDVTTFNRFFHAMLQQGVYLAPSAYEAGFVSSAHDEAVIEATLAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
REAFADVMR
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]