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The map label for this gene is tolC [H]

Identifier: 66767161

GI number: 66767161

Start: 995033

End: 996406

Strand: Reverse

Name: tolC [H]

Synonym: XC_0828

Alternate gene names: 66767161

Gene position: 996406-995033 (Counterclockwise)

Preceding gene: 66767162

Following gene: 66767159

Centisome position: 19.35

GC content: 59.39

Gene sequence:

>1374_bases
ATGATCCGCCGATCCCTCGTCCTGGCGCTGGCCGCCGCCCTGTTTCCGATGGCCGCGCACGCCACCGACCTGCTGCAGGT
CTATGAAATGGCCCGCAACGGCGATCCGCAGCTGGCTGTTGCCGAGTCCACGCGGTTGGTGAATCGGGAAGGACAAGTGC
AGGCGCGTGCGGCGTTGTTGCCACAGCTCAACGGCGCTTTCGATTACAGCAAAGCTCATCGCGAGATTGAGGGTCAGGAT
GGGCGCATTACAACTTCGTCACGCTCCAGCCAGATCCAGGGCTCACAAACCATCTTTAACTGGTCGCAATTTTCCACACT
ACGTGCACAGCGTGAAGTCGCCAAGGCAGCAGACTTCACGCTCGAGTCTGCCAACAACGACCTGATCACCCGTACCTCGG
CAGCATATTTTCAGGTATTGGTCGGCATCGAGTCACTGGCAGCGGCCGAGACCAACGAGGCAGCGGCCAAGAAGCAATTC
GACTACGCCGACAAGCGTTTAGAAGTGGGTCTGGCGCCGATCACCGACGTGCACGAAGCCCGTGCCCAGTACGACCAGGC
GCGCGCCGATACCATCAATGCACGCAACACCTTGAAGGATTACTACCAGGCCTTGACCGAACTCACCGGTCAGCCGGTGG
TGGGCCTGCGCGCGTTGCCAGAAAACTTTCGACCGGAAGTTCCTGCCGCTTACAGCAATGTCGACCAATTGGTGGCCTCG
GCTATCGCCGACAATCCGGCGCTGAAGGCCCAACAATTGCAGGTCAGCGCAGCTGAAGCGAGTATCAATGCGGCAAGGGC
AGGTCACCTTCCCACCGTCAATTTGACTGGCAGTGTGGGCCGCAGCAATAGCTGGGGTACCGGAGCCGCAGAAAGCGGTG
TCTTCACTACTCAGGGACGTGACATCGACACCGACTCGATCGGCATCAGTGTGTCGATTCCAATTTTTGCTGGTGGCGCG
ACGCAGTCGGCAGTACGTCAGGCAATTTCGCAGCGTGATATTCAGCAGGACACCTACGAACAGCAGAAACGCGCGCTGGA
TCGCAATACGCGCAATGCCTATCAGACCGTAGTAGCCGGTATCAGCGAGGTGGAAGCACGTCGCCTGTCGGTGGTGTCCG
CTCAAGCGGCTTATGACGCGTCTCAGGTCGGCCTGGAAGTGGGTACGCGCACCGTGCTGGACGTGGTGCAGAACCAGCGC
ACGTTGTTCCAGGCACAGCAGTTATATGCACAGGCGCGTTACACCTTCCTGCAGAACCGCCTGCTCCTGGCTCAGGCCAT
CGGCAAGATCGATATCGCCGAACTGCAGGACGTGAATCGCCTGCTGTCAGTAGAGGCAGAAGCCAAGCTGCAGGGCAGCG
GTTCGTTGCAGTAA

Upstream 100 bases:

>100_bases
CGCCGCGCATCGAATCGTTGTTCGAAACCGACCTCGCCTATCTCCAGGGCGCCGCACCGACGCCCCGATTCCAATTCTGA
TTCCCAAGGAAGCTTCCCCG

Downstream 100 bases:

>100_bases
GCACGTGCGCAGTGAATGAAGAGGCGGGCATGGCCCGCCTTTTCCATGTCTGCACTGTGAAAACCGGTGTTGTCATGTTT
CGGTCTCGAATCACGGCAAC

Product: TolC protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MIRRSLVLALAAALFPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALLPQLNGAFDYSKAHREIEGQD
GRITTSSRSSQIQGSQTIFNWSQFSTLRAQREVAKAADFTLESANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQF
DYADKRLEVGLAPITDVHEARAQYDQARADTINARNTLKDYYQALTELTGQPVVGLRALPENFRPEVPAAYSNVDQLVAS
AIADNPALKAQQLQVSAAEASINAARAGHLPTVNLTGSVGRSNSWGTGAAESGVFTTQGRDIDTDSIGISVSIPIFAGGA
TQSAVRQAISQRDIQQDTYEQQKRALDRNTRNAYQTVVAGISEVEARRLSVVSAQAAYDASQVGLEVGTRTVLDVVQNQR
TLFQAQQLYAQARYTFLQNRLLLAQAIGKIDIAELQDVNRLLSVEAEAKLQGSGSLQ

Sequences:

>Translated_457_residues
MIRRSLVLALAAALFPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALLPQLNGAFDYSKAHREIEGQD
GRITTSSRSSQIQGSQTIFNWSQFSTLRAQREVAKAADFTLESANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQF
DYADKRLEVGLAPITDVHEARAQYDQARADTINARNTLKDYYQALTELTGQPVVGLRALPENFRPEVPAAYSNVDQLVAS
AIADNPALKAQQLQVSAAEASINAARAGHLPTVNLTGSVGRSNSWGTGAAESGVFTTQGRDIDTDSIGISVSIPIFAGGA
TQSAVRQAISQRDIQQDTYEQQKRALDRNTRNAYQTVVAGISEVEARRLSVVSAQAAYDASQVGLEVGTRTVLDVVQNQR
TLFQAQQLYAQARYTFLQNRLLLAQAIGKIDIAELQDVNRLLSVEAEAKLQGSGSLQ
>Mature_457_residues
MIRRSLVLALAAALFPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALLPQLNGAFDYSKAHREIEGQD
GRITTSSRSSQIQGSQTIFNWSQFSTLRAQREVAKAADFTLESANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQF
DYADKRLEVGLAPITDVHEARAQYDQARADTINARNTLKDYYQALTELTGQPVVGLRALPENFRPEVPAAYSNVDQLVAS
AIADNPALKAQQLQVSAAEASINAARAGHLPTVNLTGSVGRSNSWGTGAAESGVFTTQGRDIDTDSIGISVSIPIFAGGA
TQSAVRQAISQRDIQQDTYEQQKRALDRNTRNAYQTVVAGISEVEARRLSVVSAQAAYDASQVGLEVGTRTVLDVVQNQR
TLFQAQQLYAQARYTFLQNRLLLAQAIGKIDIAELQDVNRLLSVEAEAKLQGSGSLQ

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=429, Percent_Identity=35.4312354312354, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49411; Mature: 49411

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRSLVLALAAALFPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCHHHHHHHH
PQLNGAFDYSKAHREIEGQDGRITTSSRSSQIQGSQTIFNWSQFSTLRAQREVAKAADFT
HCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHEECHHHHHHHHHHHHHHHHHHHH
LESANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQFDYADKRLEVGLAPITDVHEA
HCCCCCCEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RAQYDQARADTINARNTLKDYYQALTELTGQPVVGLRALPENFRPEVPAAYSNVDQLVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
AIADNPALKAQQLQVSAAEASINAARAGHLPTVNLTGSVGRSNSWGTGAAESGVFTTQGR
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCC
DIDTDSIGISVSIPIFAGGATQSAVRQAISQRDIQQDTYEQQKRALDRNTRNAYQTVVAG
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ISEVEARRLSVVSAQAAYDASQVGLEVGTRTVLDVVQNQRTLFQAQQLYAQARYTFLQNR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLAQAIGKIDIAELQDVNRLLSVEAEAKLQGSGSLQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHHEECCCCCCC
>Mature Secondary Structure
MIRRSLVLALAAALFPMAAHATDLLQVYEMARNGDPQLAVAESTRLVNREGQVQARAALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCHHHHHHHH
PQLNGAFDYSKAHREIEGQDGRITTSSRSSQIQGSQTIFNWSQFSTLRAQREVAKAADFT
HCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHEECHHHHHHHHHHHHHHHHHHHH
LESANNDLITRTSAAYFQVLVGIESLAAAETNEAAAKKQFDYADKRLEVGLAPITDVHEA
HCCCCCCEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RAQYDQARADTINARNTLKDYYQALTELTGQPVVGLRALPENFRPEVPAAYSNVDQLVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
AIADNPALKAQQLQVSAAEASINAARAGHLPTVNLTGSVGRSNSWGTGAAESGVFTTQGR
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCC
DIDTDSIGISVSIPIFAGGATQSAVRQAISQRDIQQDTYEQQKRALDRNTRNAYQTVVAG
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ISEVEARRLSVVSAQAAYDASQVGLEVGTRTVLDVVQNQRTLFQAQQLYAQARYTFLQNR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLAQAIGKIDIAELQDVNRLLSVEAEAKLQGSGSLQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]