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The map label for this gene is htrB [H]

Identifier: 66767159

GI number: 66767159

Start: 991951

End: 992871

Strand: Reverse

Name: htrB [H]

Synonym: XC_0826

Alternate gene names: 66767159

Gene position: 992871-991951 (Counterclockwise)

Preceding gene: 66767161

Following gene: 66767155

Centisome position: 19.28

GC content: 66.67

Gene sequence:

>921_bases
ATGTCCGAGTCCGCCAACGTCGCCGTCCGCCCTTCCCTGCGCAACCCCAAGCACTGGCCGATGTACCTGGGCCTGGCGGT
GATGGTGCTCGCCGGGCGCCTGCCGTGGCTGCTGCAACGCGCGCTTGGCCGCGGCGTTGGTTGGGTGGCGATGCGCCTGG
CGGGCACGCGCCGCCGCGCGGCCGAGGTCAACCTGAAACTGTGCTTCCCCGAGCAGGACGATGCGTGGCGGGCACGGCTG
TTACGCGACAGCTTCGACGCACTGGGCGTGGGCCTGTTCGAATTCGCACGCGCCTGGTGGGGCAGCATCGCCACCATCCG
CCCACGCATGCAGATCGAAGGCCTGGAACATCTGCAGCGGCTGCAGGAACAAAAGCGCGGCGTGCTGTTGGTGTCCGGAC
ACTTCATGACCCTGGAAATGTGCGGGCGCCTGTTGTGCGACCACGTGCCGCTGGCCGGCATGTACCGCAAACACCGCAAT
CCGGTGTTCGAGTGGGCGGTCAAGCGCGGCCGGCTGCGCTACGCCACGCACATGTTCGCCAACGAGGACCTGCGCGCCAC
CATCAAGCATCTCAAGCGCGGTGGTTTCCTGTGGTACGCGCCGGACCAGGACATGCGCGGCAAGGACACGGTCTTTGTGC
CGTTCTTCGGGCATCCGGCTTCCACCATTACCGCGACCCATCAGCTGGCGCGGCTCACCGGCTGCGCGGTGGTGCCGTAC
TTCCATCGTCGCGAAGGCGGCAGCTACGTGCTGAAGATCGCACCGCCGCTGGCGGACATCCCCTCCGACGATGTGATTGC
CGATACCACGCAGGTCAATGCGGCGATCGAGGACATGGTGCGCGAAGCGCCCGATCAATACCTGTGGATCCATCGCCGCT
TCAAGCGGCAGCCCGGTGGGCGGAGCGACTTCTATCGCTGA

Upstream 100 bases:

>100_bases
CCCTGCCAGTAGCGCTCACAACACGGTACGCGCGACACGTTGGCTGTACCGCCCACCGCCCACTCACCTCAAAGCCCGGA
GCACGCTCTAGAATTACGCC

Downstream 100 bases:

>100_bases
GGGCGCTGGCGCTGGCACTACGTGCTTGAGTGCTTGAGGTGCTTGAGTGCTTGAGTGCGGCGAGCGTCTAGCCAGGCGGG
CCGCTGACGTACGTAGTTGA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSESANVAVRPSLRNPKHWPMYLGLAVMVLAGRLPWLLQRALGRGVGWVAMRLAGTRRRAAEVNLKLCFPEQDDAWRARL
LRDSFDALGVGLFEFARAWWGSIATIRPRMQIEGLEHLQRLQEQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRKHRN
PVFEWAVKRGRLRYATHMFANEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
FHRREGGSYVLKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGGRSDFYR

Sequences:

>Translated_306_residues
MSESANVAVRPSLRNPKHWPMYLGLAVMVLAGRLPWLLQRALGRGVGWVAMRLAGTRRRAAEVNLKLCFPEQDDAWRARL
LRDSFDALGVGLFEFARAWWGSIATIRPRMQIEGLEHLQRLQEQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRKHRN
PVFEWAVKRGRLRYATHMFANEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
FHRREGGSYVLKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGGRSDFYR
>Mature_305_residues
SESANVAVRPSLRNPKHWPMYLGLAVMVLAGRLPWLLQRALGRGVGWVAMRLAGTRRRAAEVNLKLCFPEQDDAWRARLL
RDSFDALGVGLFEFARAWWGSIATIRPRMQIEGLEHLQRLQEQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRKHRNP
VFEWAVKRGRLRYATHMFANEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPYF
HRREGGSYVLKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGGRSDFYR

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=297, Percent_Identity=38.3838383838384, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082094, Length=294, Percent_Identity=37.0748299319728, Blast_Score=200, Evalue=8e-53,
Organism=Escherichia coli, GI1788161, Length=304, Percent_Identity=27.3026315789474, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35042; Mature: 34911

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESANVAVRPSLRNPKHWPMYLGLAVMVLAGRLPWLLQRALGRGVGWVAMRLAGTRRRA
CCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHH
AEVNLKLCFPEQDDAWRARLLRDSFDALGVGLFEFARAWWGSIATIRPRMQIEGLEHLQR
HEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHH
LQEQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRKHRNPVFEWAVKRGRLRYATHMFA
HHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHC
NEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
CCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEECCC
FHRREGGSYVLKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGG
HHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
RSDFYR
CCCCCC
>Mature Secondary Structure 
SESANVAVRPSLRNPKHWPMYLGLAVMVLAGRLPWLLQRALGRGVGWVAMRLAGTRRRA
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHH
AEVNLKLCFPEQDDAWRARLLRDSFDALGVGLFEFARAWWGSIATIRPRMQIEGLEHLQR
HEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHH
LQEQKRGVLLVSGHFMTLEMCGRLLCDHVPLAGMYRKHRNPVFEWAVKRGRLRYATHMFA
HHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHC
NEDLRATIKHLKRGGFLWYAPDQDMRGKDTVFVPFFGHPASTITATHQLARLTGCAVVPY
CCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEECCC
FHRREGGSYVLKIAPPLADIPSDDVIADTTQVNAAIEDMVREAPDQYLWIHRRFKRQPGG
HHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
RSDFYR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]