Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 66048277

GI number: 66048277

Start: 5995267

End: 5996271

Strand: Direct

Name: ansA [H]

Synonym: Psyr_5053

Alternate gene names: 66048277

Gene position: 5995267-5996271 (Clockwise)

Preceding gene: 66048276

Following gene: 66048281

Centisome position: 98.38

GC content: 64.18

Gene sequence:

>1005_bases
ATGCCTGATGACGCACAACAACCCGCCCAACGCGTGATGGTTCTTTACACCGGCGGCACCATCGGCATGCAGGCCAGCGC
CAACGGCCTGGCACCGGCCTCAGGTTTCGAAGCGCGAATGGCCGGGCAACTGGCCGAGCACCCGGAACTGGTGGTGCCGC
AGTGGCGCTTTCGCGAGATGTCGCCGCTGATCGACAGCGCCAACATGACGCCTTCCTACTGGCTGCGCCTGCGCGCGGCG
GTAATCGAGGCGGTTGAGGTCGACGGCTGCGATGCGGTGCTGGTCCTGCATGGCACCGACACCCTGGCGTACAGCGCAGC
GGCGATGTGCTTCCAGTTGTTGGGGTTCAGCGCGCCGGTGCTGTTCACCGGTTCGATGCTGCCTGCCGGCGTGCAGGACA
GCGATGCCTGGGAAAACGTCAACGGCGCTCTGCTGGCGCTGGGTTCGGGTCTGGTGTCAGGCGTTCAGCTGTATTTCCAC
GGGCAACTGATGACACCCGTACGCTGCGCGAAAATTCGCAGCTTCGGTCGTCAACCCTTCGCACCGCTGCAACGCTCGCG
GGGTGGCCATGCGGTGATTTCGCTCCCTGACGCACTGAACTATCGCCAGCCCAAGACACTTACAGAGGTGGCGGTATTGC
CGCTGTTTCCCGGTGTCGGTGCTGCCCAGCTGGACGGTCTGATCAACAGTGGTATCCGCGGTCTGGTGCTCGAATGCTTC
GGCAGCGGCACCGGCCCCAGTGACGATCCGCAATTTCTGGCCAGTCTGGAGAACGCACGAGCGCTGGGTATCTGTGTGGT
AGCGATTACCCAGTGCCATGAGGGCGGTGTCGAACTTGATGTCTACGAAGCGGGCAGCCGTCTGCGCAGTGCAGGCGTGC
TCTCCGGTGGTGGCATGACCCGTGAAGCAGCGCTCGGCAAACTGCATGCGCTGCTGGGCGCCGGCCTGACGACCGAAGAG
GTCCGCCGCCTGGTCGAACTGGACCTGTGCGGCGAGCTGCGTTAA

Upstream 100 bases:

>100_bases
CAGCCAATCCGACCCGTCAGTCGACGCAGGATGCCGAAGCGGCAGCCAAGCCTGCGCCCGAAGCACGATAACCACCGCGA
CACTACGCCGGAGAAGAGCC

Downstream 100 bases:

>100_bases
GCGGCTGCCTCTGCTTTACCCGAGGCGTCACGCACTCGCGCGACGCCTCACCCCTTCATGAAGCCTTGCATTCTTCCTGC
GCGCCCCTGAGCATCGAATC

Product: asparaginase

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MPDDAQQPAQRVMVLYTGGTIGMQASANGLAPASGFEARMAGQLAEHPELVVPQWRFREMSPLIDSANMTPSYWLRLRAA
VIEAVEVDGCDAVLVLHGTDTLAYSAAAMCFQLLGFSAPVLFTGSMLPAGVQDSDAWENVNGALLALGSGLVSGVQLYFH
GQLMTPVRCAKIRSFGRQPFAPLQRSRGGHAVISLPDALNYRQPKTLTEVAVLPLFPGVGAAQLDGLINSGIRGLVLECF
GSGTGPSDDPQFLASLENARALGICVVAITQCHEGGVELDVYEAGSRLRSAGVLSGGGMTREAALGKLHALLGAGLTTEE
VRRLVELDLCGELR

Sequences:

>Translated_334_residues
MPDDAQQPAQRVMVLYTGGTIGMQASANGLAPASGFEARMAGQLAEHPELVVPQWRFREMSPLIDSANMTPSYWLRLRAA
VIEAVEVDGCDAVLVLHGTDTLAYSAAAMCFQLLGFSAPVLFTGSMLPAGVQDSDAWENVNGALLALGSGLVSGVQLYFH
GQLMTPVRCAKIRSFGRQPFAPLQRSRGGHAVISLPDALNYRQPKTLTEVAVLPLFPGVGAAQLDGLINSGIRGLVLECF
GSGTGPSDDPQFLASLENARALGICVVAITQCHEGGVELDVYEAGSRLRSAGVLSGGGMTREAALGKLHALLGAGLTTEE
VRRLVELDLCGELR
>Mature_333_residues
PDDAQQPAQRVMVLYTGGTIGMQASANGLAPASGFEARMAGQLAEHPELVVPQWRFREMSPLIDSANMTPSYWLRLRAAV
IEAVEVDGCDAVLVLHGTDTLAYSAAAMCFQLLGFSAPVLFTGSMLPAGVQDSDAWENVNGALLALGSGLVSGVQLYFHG
QLMTPVRCAKIRSFGRQPFAPLQRSRGGHAVISLPDALNYRQPKTLTEVAVLPLFPGVGAAQLDGLINSGIRGLVLECFG
SGTGPSDDPQFLASLENARALGICVVAITQCHEGGVELDVYEAGSRLRSAGVLSGGGMTREAALGKLHALLGAGLTTEEV
RRLVELDLCGELR

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=365, Percent_Identity=32.6027397260274, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI1788065, Length=334, Percent_Identity=35.0299401197605, Blast_Score=184, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI193207374, Length=364, Percent_Identity=29.6703296703297, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI193207372, Length=364, Percent_Identity=29.6703296703297, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17558278, Length=364, Percent_Identity=29.6703296703297, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17558280, Length=364, Percent_Identity=29.6703296703297, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639609, Length=368, Percent_Identity=30.9782608695652, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645530, Length=377, Percent_Identity=30.7692307692308, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35172; Mature: 35041

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDDAQQPAQRVMVLYTGGTIGMQASANGLAPASGFEARMAGQLAEHPELVVPQWRFREM
CCCCHHCHHHEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCEECCCHHHHHH
SPLIDSANMTPSYWLRLRAAVIEAVEVDGCDAVLVLHGTDTLAYSAAAMCFQLLGFSAPV
HHHHCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCC
LFTGSMLPAGVQDSDAWENVNGALLALGSGLVSGVQLYFHGQLMTPVRCAKIRSFGRQPF
EEECCCCCCCCCCCCHHHCCCCEEEEHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCC
APLQRSRGGHAVISLPDALNYRQPKTLTEVAVLPLFPGVGAAQLDGLINSGIRGLVLECF
CHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHH
GSGTGPSDDPQFLASLENARALGICVVAITQCHEGGVELDVYEAGSRLRSAGVLSGGGMT
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEHHCHHHHHCCEECCCCCC
REAALGKLHALLGAGLTTEEVRRLVELDLCGELR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDDAQQPAQRVMVLYTGGTIGMQASANGLAPASGFEARMAGQLAEHPELVVPQWRFREM
CCCHHCHHHEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCEECCCHHHHHH
SPLIDSANMTPSYWLRLRAAVIEAVEVDGCDAVLVLHGTDTLAYSAAAMCFQLLGFSAPV
HHHHCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCC
LFTGSMLPAGVQDSDAWENVNGALLALGSGLVSGVQLYFHGQLMTPVRCAKIRSFGRQPF
EEECCCCCCCCCCCCHHHCCCCEEEEHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCC
APLQRSRGGHAVISLPDALNYRQPKTLTEVAVLPLFPGVGAAQLDGLINSGIRGLVLECF
CHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHH
GSGTGPSDDPQFLASLENARALGICVVAITQCHEGGVELDVYEAGSRLRSAGVLSGGGMT
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEHHCHHHHHCCEECCCCCC
REAALGKLHALLGAGLTTEEVRRLVELDLCGELR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]